Zebrafish Facilities
Published in 17/6/2022
Zebrafish lab is certified by DGAV (Direção Geral de Alimentação e Veterinária) as an authorized lab for the utilization of zebrafish (Danio rerio) in scientific research and breeding (for internal use). Zebrafish facilities include two racks of a ZebTEC (Tecniplast) recirculating system with capacity for 2400 adult fish and a WTU (Water Treatment Unit). Culture water is obtained through reverse osmosis and activated carbon filtration of tap water, complemented with 0.34 mg/L salt (Instant Ocean Synthetic Sea Salt), and automatically adjusted for temperature, pH and conductivity. A 12:12-h light: dark photoperiod is maintained. Recirculating water is treated with mechanical and biological filters as well as with UV radiation. Two Stereomicroscopes (Nikon) are available to select zebrafish eggs for testing and to track embryo development during the assays (embryo development delays and anomalies). Locomotor behavior of zebrafish larvae and juveniles is studied by automated video-tracking using the equipment Zebrabox (ViewPoint) allowing the study of behavioral components such as activity (hyper or hypo activity), thigmotaxis and erratic swimming. In compliance with the 3 R’s for ethics in animal experimentation, zebrafish embryos are preferred for testing (using procedures based in the OECD guideline 236- Fish Embryo Toxicity assay). Zebrafish facilities are mainly used by ecotoxicologists that use the zebrafish as a model to assess effects and modes of action of several types of chemical compounds. Research topics include: -Effects and modes of action of pesticides, pharmaceuticals, nanoplastics, nanomaterials, etc. -Effects of environmental stressors (temperature, salinity, pH, UV radiation) and their combined effects with chemical contaminants. - Effects of emergent contaminants in environmental realistic scenarios. -Toxicity assessment of environmental samples (e.g. effluents, ashes, mining drainage, agricultural drainage). Images
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