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title A study of the effects of citrate-coated silver nanoparticles on RAW 264.7 cells using a toolbox of cytotoxic endpoints
authors Bastos, V; Duarte, IF; Santos, C; Oliveira, H
author full name Bastos, V.; Duarte, I. F.; Santos, C.; Oliveira, H.
title A study of the effects of citrate-coated silver nanoparticles on RAW 264.7 cells using a toolbox of cytotoxic endpoints
nationality internacional
language English
document type Article
author keywords Cell cycle; Citrate-coated silver nanoparticles; Nanotoxicity; Raw 264.7. ROS and Viability; Health effects
abstract Citrate-coated silver nanoparticles (citrate-AgNPs) are among the most commonly used nanomaterials, widely present in industrial and biomedical products. In this study, the cytotoxicity of 30-nm citrate-AgNPs on the macrophage cell line RAW 264.7 was evaluated, using a battery of cytotoxicity endpoints (viability, oxidative stress, and cytostaticity/clastogenicity), at 24 and 48 h of exposure. Citrate-AgNPs decreased cell proliferation and viability only at 75 mu g/mL, suggesting a low sensitivity of RAW cells to lower doses of these AgNPs. After 24 h of exposure, ROS content decreased in cells exposed to 60 mu g/mL AgNPs (IC20 value), corroborating the high tolerance of these cells to citrateAgNPs. However, these cells suffered an impairment of the cell cycle, shown by an increase at the sub-G1 phase. This increase of the sub-G1 population was correlated with an increase of DNA fragmentation, suggesting an increase of apoptosis. Thus, our data are important to understand the effects of low concentrations (IC20) of citrate-AgNPs on in vitro vital macrophage functions.
author address [Bastos, V.; Santos, C.; Oliveira, H.] Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro, Portugal; [Duarte, I. F.] Univ Aveiro, CICECO Aveiro Inst Mat, Dept Chem, P-3810193 Aveiro, Portugal; [Santos, C.] Univ Porto, Dept Biol, Rua Campo Alegre, P-469007 Oporto, Portugal
reprint address Santos, C; Oliveira, H (reprint author), Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro, Portugal.
e-mail address csantos@fc.up.pt; holiveira@ua.pt
researcherid number Oliveira, Helena/M-5068-2013
orcid number Oliveira, Helena/0000-0002-4673-0696; Santos, Conceicao/0000-0003-4129-6381; Bastos, Veronica/0000-0002-9882-4766; Duarte, Iola/0000-0003-4289-9256
funding agency and grant number European Regional Development Fund (FEDER) through the Competitive Factors Thematic Operational Programme (COMPETE); National Funds through the Foundation for Science and Technology (FCT) [CICECO-FCOMP-01-0124FEDER-037271, FCOMP-01-0124-FEDER-021456, FCT PEst-C/CTM/LA0011/2013, FCT PTDC/SAUTOX/120953/2010]; FCT [SFRH/BD/81792/2011]; H.O. [SFRH/BPD/111736/2015]
funding text This work has been funded by the European Regional Development Fund (FEDER) through the Competitive Factors Thematic Operational Programme (COMPETE) and by National Funds through the Foundation for Science and Technology (FCT), under the projects CICECO-FCOMP-01-0124FEDER-037271 (Refa. FCT PEst-C/CTM/LA0011/2013) and FCOMP-01-0124-FEDER-021456 (Refa. FCT PTDC/SAUTOX/120953/2010). The grants awarded by FCT to V.B. (SFRH/BD/81792/2011) and H.O. (SFRH/BPD/111736/2015) are also acknowledged. I.F.D acknowledges FCT/MCTES for a research contract under the Program
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cited reference count 46
times cited 1
total times cited count (wos, bci, and cscd) 1
publisher SPRINGER
publisher city DORDRECHT
issn 1388-0764
29-character source abbreviation J NANOPART RES
iso source abbreviation J. Nanopart. Res.
publication date APR 28
year published 2017
volume 19
issue 5
article number 163
digital object identifier (doi) 10.1007/s11051-017-3855-1
subject category 9
document delivery number Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
unique article identifier Chemistry; Science & Technology - Other Topics; Materials Science