Currently in testing mode

  • Authors Bastos, V; Duarte, IF; Santos, C; Oliveira, H
  • Author Full Name Bastos, V.; Duarte, I. F.; Santos, C.; Oliveira, H.
  • Title A study of the effects of citrate-coated silver nanoparticles on RAW 264.7 cells using a toolbox of cytotoxic endpoints
  • Nationality internacional
  • Source JOURNAL OF NANOPARTICLE RESEARCH
  • Language English
  • Document Type Article
  • Author Keywords Cell cycle; Citrate-coated silver nanoparticles; Nanotoxicity; Raw 264.7. ROS and Viability; Health effects
  • Keywords Plus IN-VITRO; PARTICLE DISSOLUTION; CELLULAR-RESPONSES; SCAVENGER RECEPTOR; TOXICITY; NANOTOXICITY; GENOTOXICITY; MACROPHAGES; APOPTOSIS; SILICA
  • Abstract Citrate-coated silver nanoparticles (citrate-AgNPs) are among the most commonly used nanomaterials, widely present in industrial and biomedical products. In this study, the cytotoxicity of 30-nm citrate-AgNPs on the macrophage cell line RAW 264.7 was evaluated, using a battery of cytotoxicity endpoints (viability, oxidative stress, and cytostaticity/clastogenicity), at 24 and 48 h of exposure. Citrate-AgNPs decreased cell proliferation and viability only at 75 mu g/mL, suggesting a low sensitivity of RAW cells to lower doses of these AgNPs. After 24 h of exposure, ROS content decreased in cells exposed to 60 mu g/mL AgNPs (IC20 value), corroborating the high tolerance of these cells to citrateAgNPs. However, these cells suffered an impairment of the cell cycle, shown by an increase at the sub-G1 phase. This increase of the sub-G1 population was correlated with an increase of DNA fragmentation, suggesting an increase of apoptosis. Thus, our data are important to understand the effects of low concentrations (IC20) of citrate-AgNPs on in vitro vital macrophage functions.
  • Author Address [Bastos, V.; Santos, C.; Oliveira, H.] Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro, Portugal; [Duarte, I. F.] Univ Aveiro, CICECO Aveiro Inst Mat, Dept Chem, P-3810193 Aveiro, Portugal; [Santos, C.] Univ Porto, Dept Biol, Rua Campo Alegre, P-469007 Oporto, Portugal
  • Reprint Address Santos, C; Oliveira, H (reprint author), Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro, Portugal.
  • E-mail Address csantos@fc.up.pt; holiveira@ua.pt
  • ResearcherID Number Oliveira, Helena/M-5068-2013
  • ORCID Number Oliveira, Helena/0000-0002-4673-0696; Santos, Conceicao/0000-0003-4129-6381; Bastos, Veronica/0000-0002-9882-4766; Duarte, Iola/0000-0003-4289-9256
  • Funding Agency and Grant Number European Regional Development Fund (FEDER) through the Competitive Factors Thematic Operational Programme (COMPETE); National Funds through the Foundation for Science and Technology (FCT) [CICECO-FCOMP-01-0124FEDER-037271, FCOMP-01-0124-FEDER-021456, FCT PEst-C/CTM/LA0011/2013, FCT PTDC/SAUTOX/120953/2010]; FCT [SFRH/BD/81792/2011]; H.O. [SFRH/BPD/111736/2015]
  • Funding Text This work has been funded by the European Regional Development Fund (FEDER) through the Competitive Factors Thematic Operational Programme (COMPETE) and by National Funds through the Foundation for Science and Technology (FCT), under the projects CICECO-FCOMP-01-0124FEDER-037271 (Refa. FCT PEst-C/CTM/LA0011/2013) and FCOMP-01-0124-FEDER-021456 (Refa. FCT PTDC/SAUTOX/120953/2010). The grants awarded by FCT to V.B. (SFRH/BD/81792/2011) and H.O. (SFRH/BPD/111736/2015) are also acknowledged. I.F.D acknowledges FCT/MCTES for a research contract under the Program
  • Cited References Abdelhalim MAK, 2011, LIPIDS HLTH DIS, V10, P1; Aldossari AA, 2015, J NANOPART RES, V17, DOI 10.1007/s11051-015-3116-0; Arora S, 2008, TOXICOL LETT, V179, P93, DOI 10.1016/j.toxlet.2008.04.009; AshaRani PV, 2009, ACS NANO, V3, P279, DOI 10.1021/nn800596w; Avalos A, 2014, J APPL TOXICOL, V34, P413, DOI 10.1002/jat.2957; Bancos S, 2015, INT J NANOMED, V10, P183, DOI 10.2147/IJN.S72580; Bastos V, 2016, TOXICOL LETT, V249, P29, DOI 10.1016/j.toxlet.2016.03.005; Bastos V, 2016, J ENVIRON SCI, V51, P191; Behra R, 2013, J R SOC INTERFACE, V10, DOI 10.1098/rsif.2013.0396; Benn TM, 2008, ENVIRON SCI TECHNOL, V42, P4133, DOI 10.1021/es7032718; Browning LM, 2013, CHEM RES TOXICOL, V26, P1503, DOI 10.1021/tx400228p; Diaz-Diestra D, 2015, J NANOPART RES, V17, DOI 10.1007/s11051-015-3269-x; Eckhardt S, 2013, CHEM REV, V113, P4708, DOI 10.1021/cr300288v; de Oliveira JMPF, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0092980; Gliga AR, 2014, PART FIBRE TOXICOL, V11, DOI 10.1186/1743-8977-11-11; Grosse S, 2013, TOXICOL IN VITRO, V27, P305, DOI 10.1016/j.tiv.2012.08.024; Haase A, 2011, J PHYS C SER, V304, P12030, DOI DOI 10.1088/1742-6596/304/1/012030; Hamilton RF, 2014, INT J MOL SCI, V15, P6815, DOI 10.3390/ijms15046815; Hussain SM, 2005, TOXICOL IN VITRO, V19, P975, DOI 10.1016/j.tiv.2005.06.034; Kajstura M, 2007, CYTOM PART A, V71A, P125, DOI 10.1002/cyto.a.20357; Kaur J, 2013, FOOD CHEM TOXICOL, V51, P1, DOI 10.1016/j.fct.2012.08.044; Li Y, 2014, INT J MOL SCI, V15, P3671, DOI 10.3390/ijms15033671; Liu WT, 2006, J BIOSCI BIOENG, V102, P1, DOI 10.1263/jbb.102.1; Misra RK, 1999, CYTOMETRY, V36, P112, DOI 10.1002/(SICI)1097-0320(19990601)36:2<112::AID-CYTO4>3.0.CO;2-7; Mukherjee D, 2014, J TOXICOL, V2014; Munusamy P, 2015, BIOINTERPHASES, V10, DOI 10.1116/1.4926547; Nowack B, 2007, ENVIRON POLLUT, V150, P5, DOI 10.1016/j.envpol.2007.06.006; Nowack B, 2011, ENVIRON SCI TECHNOL, V45, P1177, DOI 10.1021/es103316q; Oberdorster Gunter, 2005, Part Fibre Toxicol, V2, P8, DOI 10.1186/1743-8977-2-8; Oliveira H, 2014, MUTAT RES-GEN TOX EN, V775, P38, DOI 10.1016/j.mrgentox.2014.10.002; Orlowski P, 2013, TOXICOL IN VITRO, V27, P1798, DOI 10.1016/j.tiv.2013.05.010; Orr GA, 2011, NANOTOXICOLOGY, V5, P296, DOI 10.3109/17435390.2010.513836; Park EJ, 2010, TOXICOL IN VITRO, V24, P872, DOI 10.1016/j.tiv.2009.12.001; Park J, 2011, CHEM COMMUN, V47, P4382, DOI 10.1039/c1cc10357a; Pratsinis A, 2013, SMALL, V9, P2576, DOI 10.1002/smll.201202120; Salata O.V., 2004, J NANOBIOTECHNOL, V2, P3, DOI DOI 10.1186/1477-3155-2-3; Sasidharan A, 2011, NANOSCALE, V3, P3657, DOI 10.1039/c1nr10272a; Shannahan JH, 2015, J NANOMATER, V501; Sharma VK, 2009, ADV COLLOID INTERFAC, V145, P83, DOI 10.1016/j.cis.2008.09.002; Smita S, 2012, ENVIRON HEALTH-GLOB, V11, DOI 10.1186/1476-069X-11-S1-S13; TWENTYMAN PR, 1987, BRIT J CANCER, V56, P279, DOI 10.1038/bjc.1987.190; Varner K, 2010, STATE SCI LIT REV EV; Wang HY, 2012, ACS NANO, V6, P7122, DOI 10.1021/nn302186n; Wang X, 2014, SMALL, V10, P385, DOI 10.1002/smll.201301597; Zhang HY, 2015, SMALL, V11, P3797, DOI 10.1002/smll.201500251; Zhang TL, 2014, YONSEI MED J, V55, P283, DOI 10.3349/ymj.2014.55.2.283
  • Cited Reference Count 46
  • Times Cited 1
  • Total Times Cited Count (WoS, BCI, and CSCD) 1
  • Publisher SPRINGER
  • Publisher City DORDRECHT
  • Publisher Address VAN GODEWIJCKSTRAAT 30, 3311 GZ DORDRECHT, NETHERLANDS
  • ISSN 1388-0764
  • Character Source Abbreviation J NANOPART RES
  • ISO Source Abbreviation J. Nanopart. Res.
  • Publication Date APR 28
  • Year Published 2017
  • Volume 19
  • Issue 5
  • Article Number 163
  • Digital Object Identifier (DOI) 10.1007/s11051-017-3855-1
  • Subject Category 9
  • Document Delivery Number Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
  • Unique Article Identifier Chemistry; Science & Technology - Other Topics; Materials Science
CESAM - Centro de Estudos do Ambiente e do Mar - Universidade de Aveiro

CESAM Funding: