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title Shewanella species as the origin of bla(OXA-48) genes: insights into gene diversity, associated phenotypes and possible transfer mechanisms
authors Tacao, M; Araujo, S; Vendas, M; Alves, A; Henriques, I
author full name Tacao, Marta; Araujo, Susana; Vendas, Maria; Alves, Artur; Henriques, Isabel
title Shewanella species as the origin of bla(OXA-48) genes: insights into gene diversity, associated phenotypes and possible transfer mechanisms
nationality internacional
source INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
language English
document type Article
author keywords Class D carbapenemases; OXA-48-like; Origin; Shewanella
keywords plus BETA-LACTAMASE; PSEUDOMONAS-AERUGINOSA; KLEBSIELLA-PNEUMONIAE; ESCHERICHIA-COLI; SOFT-TISSUE; CLASS-A; RESISTANCE; XIAMENENSIS; EMERGENCE; CARBAPENEMASES
abstract Chromosome-encoded beta-lactamases of Shewanella spp. have been indicated as probable progenitors of bla(OXA-48)-like genes. However, these have been detected in few Shewanella spp. and dissemination mechanisms are unclear. Thus, our main objective was to confirm the role of Shewanella species as progenitors of bla(OXA-48)-like genes. In silico analysis of Shewanella genomes was performed to detect bla(OXA-48)-like genes and context, and 43 environmental Shewanella spp. were characterised. Clonal relatedness was determined by BOX-PCR. Phylogenetic affiliation was assessed by 16S rDNA and gyrB sequencing. Antibiotic susceptibility phenotypes were determined. The bla(OXA-48)-like genes and genetic context were inspected by PCR, hybridisation and sequence analysis. Gene variants were cloned in Escherichia coli and MICs were determined. Shewanella isolates were screened for integrons, plasmids and insertion sequences. Analysis of Shewanella spp. genomes showed that putative bla(OXA-48)-like is present in the majority and in an identical context. Isolates presenting unique BOX profiles affiliated with 11 Shewanella spp. bla(OXA-48)-like genes were detected in 22 isolates from 6 species. Genes encoded enzymes identical to (OXA-48), OXA-204, OXA-181, and 7 new variants differing from (OXA-48) from 2 to 82 amino acids. IS1999 was detected in 24 isolates, although not in the vicinity of bla(OXA-48) genes. Recombinant E. coli strains presented altered MICs. The presence/absence of bla(OXA-48)-like genes was species-related. Gene variants encoded enzymes with hydrolytic spectra similar to (OXA-48)-like from non-shewanellae. From the mobile elements previously described in association with bla(OXA-48)-like genes, only the IS1999 was found in Shewanella, which indicates its relevance in bla(OXA-48)-like genes transfer to other hosts. (C) 2017 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.
author address [Tacao, Marta] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; Univ Aveiro, CESAM, Aveiro, Portugal
reprint address Tacao, M (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address martat@ua.pt
researcherid number Tacao, Marta/C-9785-2009; Henriques, Isabel/B-1667-2008; Alves, Artur/C-3645-2008
orcid number Tacao, Marta/0000-0001-7965-4928; Henriques, Isabel/0000-0001-7717-4939; Alves, Artur/0000-0003-0117-2958
funding agency and grant number Fundacao para a Ciencia e Tecnologia [WaterJPI/0002/2013, IF/00492/2013, IF/00835/2013, SFRH/BPD/114855/2016, SFRH/BD/52573/2014]; national funds (PIDDAC); FEDER; [UID/AMB/50017-POCI-01-0145-FEDER-007638]
funding text This work was supported by Fundacao para a Ciencia e Tecnologia through project StARE (WaterJPI/0002/2013) and grants IF/00492/2013 (I. Henriques), IF/00835/2013 (A. Alves), SFRH/BPD/114855/2016 (M. Tacao) and SFRH/BD/52573/2014 (S. Araujo). Thanks are due also for the financial support to CESAM (UID/AMB/50017-POCI-01-0145-FEDER-007638), to FCT/MCTES through national funds (PIDDAC), and the co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020.
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cited reference count 41
publisher ELSEVIER SCIENCE BV
publisher city AMSTERDAM
publisher address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
issn 0924-8579
29-character source abbreviation INT J ANTIMICROB AG
iso source abbreviation Int. J. Antimicrob. Agents
publication date MAR
year published 2018
volume 51
issue 3
beginning page 340
ending page 348
digital object identifier (doi) 10.1016/j.ijantimicag.2017.05.014
subject category 9
document delivery number Infectious Diseases; Microbiology; Pharmacology & Pharmacy
unique article identifier Infectious Diseases; Microbiology; Pharmacology & Pharmacy