Currently in testing mode

  • Authors Maria, VL; Ribeiro, MJ; Guilherme, S; Soares, AMVM; Scott-Fordsmand, JJ; Amorim, MJB
  • Author Full Name Maria, Vera L.; Ribeiro, Maria Joao; Guilherme, Sofia; Soares, Amadeu M. V. M.; Scott-Fordsmand, Janeck J.; Amorim, Onica J. B.
  • Title Silver (nano)materials Cause Genotoxicity In Enchytraeus crypticus, As Determined By the Comet Assay
  • Nationality internacional
  • Source ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
  • Language English
  • Document Type Article
  • Author Keywords Single-cell gel electrophoresis (SCGE); Genetic damage indicator (GDI); Metallic nanoparticle; Soil invertebrate
  • Keywords Plus ANGUILLA-ANGUILLA L.; DNA-DAMAGE; CAENORHABDITIS-ELEGANS; HEDISTE DIVERSICOLOR; OXIDATIVE STRESS; BLOOD-CELLS; NANOPARTICLES; MECHANISMS; TOXICITY; AG
  • Abstract Enchytraeids have been used in standard ecotoxicity testing for approximately 20 yr. Since adopting the standard test for survival and reproduction, a number of additional tools have been developed, including transcriptomics and enzymatic biomarkers. So far, a genotoxicity tool and endpoint have not been used; hence, the goals of the present study included optimization of the in vivo alkaline comet assay in Enchytraeus crypticus. Further, the effect of silver nanomaterial (Ag NM300K, dispersed, 15 nm) was tested and compared with silver nitrate. Hydrogen peroxide was used as a positive control. The various steps were optimized. The fully detailed standard operating procedure is presented. Silver materials caused genotoxicity, this being differentiated for the nano and non-nano forms. Silver nitrate caused genotoxicity after 3 d of exposure in a dose-related manner, although after 7 d the effects were either reduced or repaired. Ag NM300K caused higher genotoxicity after 7 d for the lowest concentration, highlighting a potential nonmonotonic dose-response effect. Overall, the comet assay showed the power to discriminate effects between materials and also toxicity at low relevant doses. (C) 2017 SETAC
  • Author Address [Maria, Vera L.; Ribeiro, Maria Joao; Guilherme, Sofia; Soares, Amadeu M. V. M.; Amorim, Onica J. B.] Univ Aveiro, Dept Biol, Aveiro, Portugal; [Maria, Vera L.; Ribeiro, Maria Joao; Guilherme, Sofia; Soares, Amadeu M. V. M.; Amorim, Onica J. B.] Univ Aveiro, CESAM, Aveiro, Portugal; [Ribeiro, Maria Joao; Scott-Fordsmand, Janeck J.] Aarhus Univ, Dept Biosci, Silkeborg, Denmark
  • Reprint Address Maria, VL (reprint author), Univ Aveiro, Dept Biol, Aveiro, Portugal.; Maria, VL (reprint author), Univ Aveiro, CESAM, Aveiro, Portugal.
  • E-mail Address vmaria@ua.pt
  • ResearcherID Number Maria, Vera Lucia/I-5142-2013; Soares, Amadeu/A-8304-2008
  • ORCID Number Maria, Vera Lucia/0000-0002-0331-3489; Soares, Amadeu/0000-0003-0879-9470; Guilherme, Sofia/0000-0002-3238-4462
  • Funding Agency and Grant Number European Commission FP7 MARINA (G.A.) [263215]; FEDER through COMPETE Programa Operacional Factores de Competitividade; FCT-Fundacao para a Ciencia e Tecnologia; FEDER within the PT Partnership Agreement; Compete within the research project NM_OREO [POCI-01-0145-FEDER-016771, PTDC/AAG-MAA/4084/2014, UID/AMB/50017]; FCT [SFRH/BPD/95093/2013, SFRH/BD/95027/2013, SFRH/BPD/88947/2012]
  • Funding Text The present study was funded by the European Commission FP7 MARINA (G.A. no. 263215), by FEDER through COMPETE Programa Operacional Factores de Competitividade, and by national funding through FCT-Fundacao para a Ciencia e Tecnologia, with cofunding by FEDER within the PT2020 Partnership Agreement and Compete 2020 within the research project NM_OREO (POCI-01-0145-FEDER-016771, PTDC/AAG-MAA/4084/2014) and to CESAM (UID/AMB/50017). The FCT grants were to V.L. Maria (SFRH/BPD/95093/2013), M.J. Ribeiro (SFRH/BD/95027/2013), and S. Guilherme (SFRH/BPD/88947/2012).
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  • Cited Reference Count 49
  • Publisher WILEY
  • Publisher City HOBOKEN
  • Publisher Address 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
  • ISSN 0730-7268
  • Character Source Abbreviation ENVIRON TOXICOL CHEM
  • ISO Source Abbreviation Environ. Toxicol. Chem.
  • Publication Date JAN
  • Year Published 2018
  • Volume 37
  • Issue 1
  • Beginning Page 184
  • Ending Page 191
  • Digital Object Identifier (DOI) 10.1002/etc.3944
  • Subject Category 8
  • Document Delivery Number Environmental Sciences; Toxicology
  • Unique Article Identifier Environmental Sciences & Ecology; Toxicology
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