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  Cláudia Oliveira  
  Phone: +351 234 370774  
  e-mail: csoliveira@ua.pt  
  Category: Invited Assistant Professor  
  Department: Biology Department  
  Research group: Stress Biology
 
ISI Web of KnowledgeSM search factor: RID=A-7859-2012  
  Direct URL for this page: http://www.cesam.ua.pt/csoliveira  
     
Research interests

Experience in


Protein purification; Protein stability; Molecular microbiology


Research interests


Plasmid mediated gene transfer in environmental samples; Plasmid roles in microbial communities adaptation; Variability of plasmid associated genetic features.


Other interests


Antimicrobial activity determinations of natural and synthetic composites.

Academic degrees

BSc in Biology, University of Aveiro, 1997 MSc in Molecular Microbiology, University of Aveiro, 2001 PhD in Biology, University of Aveiro, 2007 Post Doc in Molecular Microbiology, University of Aveiro (SFRH/BPD/65820/2009) - March 2010-February 2013 Molecular Microbiology Lab http://biomicrolab.web.ua.pt/

Research Projects

2010-2013: "SEAGULL - Identification of nonpoint sources of faecal pollution in a natural environment: contributing data for risk assessment" (PTDC/AAC-AMB/109155/2008). Principal Investigator: Anabela Pereira, University of Aveiro.


2005-2008: "Assessing conformational stability of aspartic proteinases with biotechnological and medical relevance - Unfolding/refolding of dimeric and monomeric proteins" (POCI/QUI/60791/2004). Principal Investigator: Marlene Barros, Portuguese Catholic University.


1999-2001: "How do Organic Solvents Affect Cardosin A and Cardosin B Activity and Structure. The use of Cardosins in New Strategies and New Tactics for Routine Peptide Synthesis" (Praxis/P/Qui/10131/98). Principal Investigator: Marlene Barros, University of Aveiro.

Scientific Supervision
Post-doc (Main Supervisor): Sónia Cristina Santos Martins
(not a CESAM member)

Master (Main Supervisor): Beatriz Lázaro Pinto
(not a CESAM member)
Master (Co-supervisor): Maria Teresa de Sousa Coelho Cortez
(not a CESAM member)



Projects
GEMOVE: Genes in motion: Mobile genes in microbial communities, how to catch them? [see details]

PhytoMarsh - The phytosphere of salt-marsh plants: an underexplored microbial hot-spot [see details]

SEAGULL- Identification of nonpoint sources of faecal pollution in a natural environment: contributing data for risk assessment [see details]

Assessing conformational stability of aspartic proteinases with biotechnological and medical relevance – Unfolding/refolding of dimeric and monomeric proteins [see details]

Publications
  1. M.N. Capela, D.M. Tobaldi, C. Oliveira, A. Pereira, A.S. Duarte, M.P. Seabra, M.H.V. Fernandes (2017) Bioactivity and antibacterial activity against E. coli of calcium-phosphatebased glasses: Effect of silver content and crystallinity. Ceramics International. 43, 16, 13800-13809. (see details) ISI paper
  2. Henriques I., Tacao M., Leite L., Fidalgo C., Araujo S., Oliveira C., Alves A. (2016) Co-selection of antibiotic and metal(loid) resistance in gram-negative epiphytic bacteria from contaminated salt marshes. Marine Pollution Bulletin. 109, 1, 427-434. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.marpolbul.2016.05.031
  3. Seabra M.P., Grave L., Oliveira C., Alves A., Correia A., Labrincha J.A. (2014) Porcelain stoneware tiles with antimicrobial action. Ceramics International. 40, 4, 6063-6070. 1873-3956. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.ceramint.2013.11.056
  4. Azevedo J.S.N., Araújo S., Oliveira C.S., Correia A., Henriques I. (2013) Analysis of antibiotic resistance in bacteria isolated from the surface microlayer and underlying water of an estuarine environment. Microbial Drug Resistance. 19, 64-71. (see details) ISI paper
  5. Oliveira C.S, Moura A., Henriques I., Brown C.J., Rogers L.M., Top E.M., Correia A. (2013) Comparative genomics of IncP-1 epsilon plasmids from water environments reveals diverse and unique accessory genetic elements. Plasmid. 70, 3, 412–419 (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.plasmid.2013.06.002
  6. Oliveira C.S., Lázaro B., Azevedo J.N., Henriques I., Almeida A., Correia A. (2012) New Molecular Variants of Epsilon and Beta IncP-1 Plasmids are Present in Estuarine Waters. Plasmid. 67, 252-258. (see details) ISI paper
  7. Azevedo J.S.N., Ramos I., Araújo S., Oliveira C.S., Correia A., Henriques I. (2012) Spatial and temporal analysis of estuarine bacterioneuston and bacterioplankton using culture-dependent and culture-independent methodologies. Antonie Van Leeuwenhoek. 101, 819-835. (see details) ISI paper
  8. Moura A., Oliveira C., Henriques I., Smalla K., Correia A. (2012) Broad diversity of conjugative plasmids in integron-carrying bacteria from wastewater environments. Fems Microbiology Letters. 330, 2, 157-164. (see details) ISI paper
    Link: http://dx.doi.org/10.1111/j.1574-6968.2012.02544.x
  9. Sarmento A.C., Oliveira C.S., Pereira A.O., Esteves V.I., Moir A.J.G., Saraiva J., Pires E., Barros M. (2009) Unfolding of cardosin A in organic solvents and detection of intermediaries. Journal Of Molecular Catalysis B: Enzymatic. 57, 115-122. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.molcatb.2008.08.004
  10. Cardoso T., Oliveira C., Sarmento A.C., Pereira A., Nutley M.A., Jess T., Kelly S.M., Cooper A., Price N.C., Pires E., Barros M. (2009) Acetonitrile-induced unfolding of porcine pepsin A. A proposal for a critical role of hydration structures in conformational stability. International Journal Of Biological Macromolecules. 45, 3, 213-220. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.ijbiomac.2009.05.006
  11. Sarmento A.C., Lopes H., Oliveira C.S., Vitorino R., Samyn B., Sergeant K., Debyser G., van Beeumen J., Domingues P., Amado F., Pires E., Domingues M.R.M., Barros M.T. (2009) Multiplicity of aspartic proteinases from Cynara cardunculus L. Planta. 230, 429-439. (see details) ISI paper
    Link: http://www.springerlink.com/content/b08262j277872663/fulltext.pdf
  12. Oliveira C.S., Sarmento A.C., Pereira A., Correia I., Pessoa J.C., Esteves V.I., Fonseca H., Pires E., Barros M. (2009) Non-native states of cardosin A induced by acetonitrile: activity modulation via polypeptide chains rearrangements. Journal Of Molecular Catalysis B: Enzymatic. 61, 274-278. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.molcatb.2009.08.003
  13. Sarmento AC, Oliveira CS, Duarte AS, Pires E, Barros MT (2006) Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments - complex substrates hydrolysis. Enzyme And Microbial Technology. 38, 3/4, 415-421. (see details) ISI paper
  14. Shnyrova A.V., Oliveira C.S., Sarmento A.C., Barros M.T., Zhadan G.G., Roig M.G., Shnyrov V.L. (2006) Effect of acetonitrile on Cynara cardunculus L. cardosin A stability. Int J Biol Macromol. 39, 4-5, 273-9. (see details) ISI paper
  15. Sarmento A.C., Oliveira C.S., Pires E.M., Halling P.J., Barros M.T. (2004) Evaluation of cardosin A as a proteolytic probe in the presence of organic solvents. Journal Of Molecular Catalysis B: Enzymatic. . 31, 4-6, 137-141. (see details) ISI paper
  16. Sarmento A.C., Oliveira C.S., Pires E.M., Amado F, Barros M.T. (2004) Reverse hydrolysis by cardosin A. Specificity considerations. Journal Of Molecular Catalysis B: Enzymatic.. 28, 1, 33-37. (see details) ISI paper
  17. Sarmento A.C., Oliveira C.S., Pires E.M., Halling P.J., Barros M.T. (2003) Cardosin A as a model aspartic proteinase for the study of organic solvent effects. I. An overview on catalytic and structural aspects. Journal Of Molecular Catalysis. B: Enzymatic. 21, 1-2, 19-23. (see details) ISI paper
  18. Pina D.G., Oliveira C.S., Sarmento A.C., Barros M.T., Pires E.M., Zhadan G.G., Villar E., Gavilanes F., Shnyrov V.L (2003) Thermostability of cardosin A from Cynara cardunculus L. Thermochimica Acta. 71218, 1-12. (see details) ISI paper
  1. Lara Torres Conde (2017) Diversidade de Plasmídeos no ambiente e desenvolvimento de ferramentas moleculares para a sua caracterização. MSc Thesis. (see details)
  2. Lara Torres Conde (2017) Diversidade de Plasmídeos no ambiente e desenvolvimento de ferramentas moleculares para a sua caracterização. MSc Thesis. (see details)
  3. Pinto B.L. (2010) Plasmídeos com Gama Alargada de Hospedeiros em Ambientes Estuarinos. MSc Thesis. (see details)
    Link: http://hdl.handle.net/10773/3515
  4. Cortez M.T.S.C. (2009) Abordagem molecular na resolução de problemas com biocorrosão. MSc Thesis. (see details)
    Link: http://ria.ua.pt/handle/10773/845
  5. Oliveira C.S. (2007) Estados conformacionais da cardosina A. PhD Thesis. (see details)
    Link: http://ria.ua.pt/handle/10773/927
  6. Oliveira C.S. (2001) Efeito dos solventes orgânicos na estrutura secundária da cardosina A. MSc Thesis. (see details)
  1. Martins S., Moura A., Oliveira C., Correia A. (2013) The gene pool of sequenced plasmids – what can we learn?. (see details)
  2. Oliveira C.S, Moura A., Henriques I., Rogers L., Brown C, Top E., Correia A (2012) Comparative genomics and resistance phenotypes of IncP-1epsilon plasmids from estuarine waters. (see details)
  3. Oliveira C.S, Lázaro B., Henriques, I., Almeida A., Correia, A. (2011) Epsilon and beta incP-1 plasmids are present in estuarine aquatic environments, a new source of BHR plasmids. (see details)
  4. Oliveira C.S, Almeida A., Correia A. (2010) Does the surface microlayer environment select for specific microbial communities? Investigating the role of horizontal gene transfer. (see details)
  5. Azevedo J., Ramos I., Oliveira C., Henriques I., Correia A. (2009) Unraveling differences between bacterioplankton and bacterioneuston: universal or specific primers?. (see details)
  6. Oliveira C.S, Bresciani V., Almeida A., Correia A. (2009) Conjugative gene transfer and BHR plasmids incidence from the SML and subsurface waters: an estuary from Portugal as a case study. (see details)
  7. Oliveira C., Nina J., Moura A., Henriques I., Correia A. (2008) Bacterioneuston versus bacterioplankton: culture independent approach to screen for broad host range plasmids, antibiotic resistance and integron gene sequences. (see details)
  8. Sarmento A.C., Oliveira C.S., Pereira A., Pires E.M., Barros M.T. (2007) A case study: cardosin A as a model for aspartic proteinases conformational stability. (see details)
  9. Sarmento A.C., Oliveira Cláudia S., Pereira A. O., Esteves Valdemar, Saraiva Jorge, Correia Isabel, Pessoa João Costa, Pires Euclides, Barros Marlene (2006) Using Cardosina A as a Model protein. (see details)
  10. Silva P., Sarmento A.C., Oliveira C.S., Pires E., Barros M. (2006) Conformational effect of acetonitrile on an aspartic proteinase, Cardosin A. (see details)
  11. Sarmento A.C., Oliveira C.S., Pereira A. O., Pires E., Barros M. (2006) Using organic solvents to characterise conformational intermediaries of protein unfolding. (see details)
  12. Sarmento A.C., Oliveira C.S., Pereira A. O., Cardoso T., Barros M. (2005) Old techniques with nowadays applications and promising perspectives. (see details)
  13. Sarmento A.C., Lopes H., Oliveira C.S., Vitorino R., Pires E., Domingues P., Domingues M.R., Barros M. (2005) Purification and characterisation of aspartic proteinases from C. cardunculus L. (see details)
  14. Sarmento A.C., Pereira A.O., Oliveira C.S., Pina D., Shnyrova A., Shnyrov V., Pires E., Barros M.T. (2004) A conformational and functional approach: effects of acetonitrile and chemical denaturants on an aspartic proteinase, cardosin A. (see details)
  15. Cardoso T., Sarmento A.C., Oliveira C.S., Pires E., Barros M. (2004) Understanding the effects of acetonitrile on pepsin stability. (see details)
  16. CS Oliveira, , AC Sarmento, DG Pina, H Fonseca, E Pires, V Shnyrov, M Barros (2003) Stability of cardosin A from Cynara cardunculus L: a dimeric aspartic proteinase. (see details)
  17. Sarmento A.C., Oliveira C.S., Pires E., Barros M. (2003) The potential of cardosin A as a proteolytic probe. (see details)
  18. Sarmento A.C., Oliveira C.S., Cardoso T., Fonseca H., Pires E., Barros M. (2002) How proteins can be characterised using organic solvents. (see details)
  19. Sarmento A.C., Oliveira C.S., Halling P., Pires E., Barros M. (2002) Studies of enzyme behaviour in non-conventional environments. (see details)
  20. Oliveira C.S., Sarmento A.C., Pina D., Shnyrov V., Fonseca H., Pires E., Barros M. (2002) Limited proteolysis in mixed organic-aqueous solvent systems. A new tool for protein structure assessment. (see details)
  21. Pina D., Oliveira C.S., Sarmento A.C., Barros M., Villar E., Shnyrov V.L. (2002) Desnaturalización térmica y química de las cardosinas A, B y A0. (see details)
  22. Oliveira C. (2001) Conformational Study of cardosine A in organic solvents by limited proteolysis and FTIR spectroscopy. (see details)
  23. Sarmento A.C., Oliveira C.S., Pina D., Shnyrov V., Halling P.J., Pires E., Villar E., Barros M.T. (2001) Cardosin A as a model aspartic proteinase for studies of enzyme behaviour in the presence of organic solvents. (see details)
  24. Oliveira C.S., Sarmento A.C., Pina D., Shnyrov V., Pires E., Barros M. (2001) An overview of structural stability studies of proteins: cardosin A a plant aspartic proteinase with antifibrotic potential. (see details)
  25. Pina D., Oliveira C.S., Sarmento A.C., Barros M., Villar E., Shnyrov V. (2001) Structural stability of cardosin A an aspartic proteinase from Cynara cardunculus L. (see details)
  26. Oliveira C.S., Sarmento A.C., Esteves V., Duarte A., Pina D., Shnyrov V., Pires E., Barros M. (2001) Conformational study of cardosin A in organic solvents by limited proteolysis and FTIR spectroscopy. (see details)
  27. Oliveira C.S., Sarmento A.C., Pires E., Barros M. (2000) Towards an Understanding of Cardosin A Behaviour in Biphasic Systems. (see details)
  28. Sarmento A.C., Barros M., Pires E. (1998) The Application of two Aspartic Proteinases, Cardosins A and B on Peptide Synthesis. (see details)
  29. Araújo S., Tacão M., Leite L., Oliveira C., Alves A., Henriques I. Effect of metal contamination on antibiotic resistance traits in epiphytic bacteria. . (see details)
  30. Araújo S., Tacão M., Leite L., Oliveira C., Alves A., Henriques I. Effect of metal contamination on antibiotic resistance traits in epiphytic bacteria. (see details)
  1. Sarmento A.C., Lopes H., Oliveira C.S., Vitorino R., Pires E.M., Domingues P., Domingues M.R., Barros M.T. (2005) Purification and characterisation of aspartic proteinases from C. cardunculus L.. FEBS J. 272 Suppl 1. (see details)
  2. Oliveira C.S., Sarmento A.C., Pina D., Shnyrov V., Pires E., Barros M. (2001) Structural stability characterisation of cardosin A from Cynara cardunculus L.: enzymic, spectroscopic and calorimetric studies. Arquivos de Patologia, vol. XXXIII supp. (see details)