HOME  »  Members  »  Integrated  »  Faculty
 
title Novel Linear Polymers Able to Inhibit Bacterial Quorum Sensing
authors Cavaleiro, E; Duarte, AS; Esteves, AC; Correia, A; Whitcombe, MJ; Piletska, EV; Piletsky, SA; Chianella, I
author full name Cavaleiro, Eliana; Duarte, Ana Sofia; Esteves, Ana Cristina; Correia, Antonio; Whitcombe, Michael J.; Piletska, Elena V.; Piletsky, Sergey A.; Chianella, Iva
title Novel Linear Polymers Able to Inhibit Bacterial Quorum Sensing
nationality internacional
source MACROMOLECULAR BIOSCIENCE
language English
document type Article
author keywords Aeromonas hydrophila; copolymers; quorum sensing; Vibrio fischeri
keywords plus N-ACYLHOMOSERINE LACTONES; GRAM-NEGATIVE BACTERIA; TO-CELL COMMUNICATION; GENE-EXPRESSION; BIOFILM FORMATION; VIBRIO-FISCHERI; VIRULENCE; ASSAY; PREVENTION; RESISTANCE
abstract Bacterial phenotypes, such as biofilm formation, antibiotic resistance and virulence expression, are associated with quorum sensing. Quorum sensing is a density-dependent regulatory system of gene expression controlled by specific signal molecules, such as N-acyl homoserine lactones (AHLs), produced and released by bacteria. This study reports the development of linear polymers capable to attenuate quorum sensing by adsorption of AHLs. Linear polymers were synthesized using MMA as backbone monomer and methacrylic acid and itaconic acid as functional monomers. Two different quorum sensing-controlled phenotypes, Vibrio fischeri bioluminescence and Aeromonas hydrophila biofilm formation, were evaluated to test the polymers' efficiency. Results showed that both phenotypes were significantly affected by the polymers, with the itaconic acid-containing material being more effective than the methacrylic acid one. The polymer inhibitory effects were reverted by the addition of lactones, confirming attenuation of quorum sensing through sequestration of signal molecules. The polymers also showed no cytotoxicity when tested using a mammalian cell line.
author address [Cavaleiro, Eliana; Duarte, Ana Sofia; Esteves, Ana Cristina; Correia, Antonio] Univ Aveiro, Dept Biol, P-3800 Aveiro, Portugal; [Cavaleiro, Eliana; Duarte, Ana Sofia; Esteves, Ana Cristina; Correia, Antonio] Univ Aveiro, CESAM, P-3800 Aveiro, Portugal; [Cavaleiro, Eliana; Chianella, Iva] Cranfield Univ, Ctr Biomed Engn, SATM, Cranfield MK43 0AL, Beds, England; [Whitcombe, Michael J.; Piletska, Elena V.; Piletsky, Sergey A.] Univ Leicester, Dept Chem, Leicester LE1 7RH, Leics, England
reprint address Duarte, AS (reprint author), Univ Aveiro, Dept Biol, P-3800 Aveiro, Portugal.
e-mail address asduarte@ua.pt
funding agency and grant number FEDER funding through COMPETE program; national funds through FCT within the projects PROMETHEUS [PTDC/AGR-CFL/113831/2009, FCOMP-01-0124-FEDER-014096]; PEst [PEst-C/MAR/LA0017/2013]; FCT [BD/47502/2008, BPD/38008/2007, BPD/46290/2008]
funding text This work was partially supported by FEDER funding through COMPETE program and by national funds through FCT within the projects PROMETHEUS (PTDC/AGR-CFL/113831/2009 and FCOMP-01-0124-FEDER-014096) and PEst (PEst-C/MAR/LA0017/2013). FCT also financed Ph.D. and post-doctoral fellowships of Eliana Cavaleiro (BD/47502/2008), Ana Cristina Esteves (BPD/38008/2007) and Ana Sofia Duarte (BPD/46290/2008). The authors wish to thank Professor Paula Gonc, alves (Department of Biology, University of Aveiro) for the use of cell culture facilities.
cited references Ammerman N. C., 2008, CURR PROTOC MICROBIO, V2008, DOI [10.1002/9780471729259.mca04es11, DOI 10.1002/9780471729259.MCA04ES11]; Antunes LCM, 2010, MICROBIOL-SGM, V156, P2271, DOI 10.1099/mic.0.038794-0; Antunes LCM, 2009, CRIT REV MICROBIOL, V35, P69, DOI 10.1080/10408410902733946; Bassler BL, 1999, CURR OPIN MICROBIOL, V2, P582, DOI 10.1016/S1369-5274(99)00025-9; Bassler BL, 2006, CELL, V125, P237, DOI 10.1016/j.cell.2006.04.001; Boyen F, 2009, VET MICROBIOL, V135, P187, DOI 10.1016/j.vetmic.2008.12.025; Carvalho MJ, 2012, INT J FOOD MICROBIOL, V159, P230, DOI 10.1016/j.ijfoodmicro.2012.09.008; Chianella I, 2013, ANAL CHEM, V85, P8462, DOI 10.1021/ac402102j; Cruz A, 2013, ANTON LEEUW INT J G, V104, P385, DOI 10.1007/s10482-013-9961-x; Decho AW, 2010, TRENDS MICROBIOL, V18, P73, DOI 10.1016/j.tim.2009.12.008; Defoirdt T, 2008, J APPL MICROBIOL, V104, P1480, DOI 10.1111/j.1365-2672.2007.03672.x; Defoirdt T., 2010, PLOS PATHOG, V6, P7; Dickschat JS, 2010, NAT PROD REP, V27, P343, DOI 10.1039/b804469b; Djordjevic D, 2002, APPL ENVIRON MICROB, V68, P2950, DOI 10.1128/AEM.68.6.2950-2958.2002; Duarte A., LETT APPL M IN PRESS; Duarte A. S., CRIT REV MI IN PRESS; ENGEBRECHT J, 1983, CELL, V32, P773, DOI 10.1016/0092-8674(83)90063-6; Fuqua C, 2001, ANNU REV GENET, V35, P439, DOI 10.1146/annurev.genet.35.102401.090913; FUQUA WC, 1994, J BACTERIOL, V176, P269; Girod M, 2013, INT J MASS SPECTROM, V333, P27, DOI 10.1016/j.ijms.2012.08.018; Gottenbos B, 2001, J ANTIMICROB CHEMOTH, V48, P7, DOI 10.1093/jac/48.1.7; Hong Kunlun, 2002, Chem Commun (Camb), P1368, DOI 10.1039/b204319j; Kievit T. R. D. E., 2001, APPL ENVIRON MICROB, V67, P1865; Kollef MH, 2011, CLIN INFECT DIS, V53, pS33, DOI 10.1093/cid/cir475; Landini P, 2010, APPL MICROBIOL BIOT, V86, P813, DOI 10.1007/s00253-010-2468-8; Lynch MJ, 2002, ENVIRON MICROBIOL, V4, P18, DOI 10.1046/j.1462-2920.2002.00264.x; Mariscal A, 2009, APPL MICROBIOL BIOT, V82, P773, DOI 10.1007/s00253-009-1879-x; McClean KH, 1997, MICROBIOL-UK, V143, P3703; Mccoy CP, 2012, BIOMATERIALS, V33, P7952, DOI 10.1016/j.biomaterials.2012.07.052; Merritt J. H., 2011, CURR PROTOC MICROBIO; Miller M. B., 2001, ANNU REV MICROBIOL, V55, P169; Morin D, 2003, J CHROMATOGR A, V1002, P79, DOI 10.1016/S0021-9673(03)00730-1; Muniesa M, 2012, APPL ENVIRON MICROB, V78, P4065, DOI 10.1128/AEM.00217-12; Nadell C. D., 2008, PLOS BIOL, V6, P1; Nealson K. H., 1978, METHOD ENZYMOL, V57C, P153; Pettit RK, 2005, ANTIMICROB AGENTS CH, V49, P2612, DOI 10.1128/AAC.49.7.2612-2617.2005; Piletska EV, 2010, BIOMACROMOLECULES, V11, P975, DOI 10.1021/bm901451j; Piletska EV, 2011, BIOMACROMOLECULES, V12, P1067, DOI 10.1021/bm101410q; Pina S, 2010, J BIOMED MATER RES B, V94B, P414, DOI 10.1002/jbm.b.31669; Rutherford S. T., 2012, COLD SPRING HARB PER, V2, P11; Sayem S M Abu, 2011, Microb Cell Fact, V10, P74, DOI 10.1186/1475-2859-10-74; Waters CM, 2005, ANNU REV CELL DEV BI, V21, P319, DOI 10.1146/annurev.cellbio.21.012704.131001; Whitehead NA, 2001, FEMS MICROBIOL REV, V25, P365, DOI 10.1111/j.1574-6976.2001.tb00583.x; Williams P, 2007, MICROBIOL-SGM, V153, P3923, DOI 10.1099/mic.0.2007/012856-0; Winzer K, 2001, INT J MED MICROBIOL, V291, P131, DOI 10.1078/1438-4221-00110; ZHANG LH, 1993, NATURE, V362, P446, DOI 10.1038/362446a0
cited reference count 46
publisher WILEY-V C H VERLAG GMBH
publisher city WEINHEIM
publisher address BOSCHSTRASSE 12, D-69469 WEINHEIM, GERMANY
issn 1616-5187
29-character source abbreviation MACROMOL BIOSCI
iso source abbreviation Macromol. Biosci.
publication date MAY
year published 2015
volume 15
issue 5
beginning page 647
ending page 656
digital object identifier (doi) 10.1002/mabi.201400447
page count 10
web of science category Biochemistry & Molecular Biology; Materials Science, Biomaterials; Polymer Science
subject category Biochemistry & Molecular Biology; Materials Science; Polymer Science
document delivery number CI0AB
unique article identifier WOS:000354396800006
link http://dx.doi.org/10.1002/mabi.201400447