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title Development of an embryotoxicity test for Enchytraeus crypticus - The effect of Cd
authors Goncalves, MFM; Bicho, RC; Rema, A; Soares, AMVM; Faustino, AMR; Amorim, MJB
author full name Goncalves, Micael F. M.; Bicho, Rita C.; Rema, Alexandra; Soares, Amadeu M. V. M.; Faustino, Augusto M. R.; Amorim, Monica J. B.
title Development of an embryotoxicity test for Enchytraeus crypticus - The effect of Cd
nationality internacional
source CHEMOSPHERE
language English
document type Article
author keywords Embryonic development; Cadmium; Embryotoxicity; Histology; Immunohistochemistry
keywords plus CALCIUM-TRANSPORT PROTEINS; SLUG DEROCERAS-RETICULATUM; CADMIUM UPTAKE; EXTRAEMBRYONIC MEMBRANES; EMBRYONIC-DEVELOPMENT; LYMNAEA-STAGNALIS; EGGS; EMBRYOGENESIS; EXPRESSION; PESTICIDES
abstract The existing standard enchytraeid reproduction test (ERT) concerns the assessment of effects on survival and reproduction. In the present study we optimized and propose an embryotoxicity test using Enchytraeus crypticus. Cadmium (Cd) was used as a test substance. Endpoints evaluated were embryo development, number of embryonic structures, Calcium (Ca) channels quantification and hatching success with macroscopic monitoring, histological and immunohistochemistry analysis. Results showed that Cd is embryotoxic for this species, causing a decrease in the hatching success (EC50 = 3.1 mg/kg), a delay or disruption in formation of embryonic structures depending on concentrations (<5 mg Cd/kg or >= 16 mg Cd/kg). Results from immunohistochemistry suggest a competitive binding between Cd and Ca for Ca channels, resulting in changes in Ca homeostasis. The use of the E. crypticus embryotoxicity test with the combination of histological and immunohistological tools provided a good option towards mechanistic information enhancing the importance of these tests to evaluate the hazard of chemicals and possible use in risk assessment. (C) 2015 Elsevier Ltd. All rights reserved.
author address [Goncalves, Micael F. M.; Bicho, Rita C.; Soares, Amadeu M. V. M.; Amorim, Monica J. B.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Goncalves, Micael F. M.; Bicho, Rita C.; Soares, Amadeu M. V. M.; Amorim, Monica J. B.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Rema, Alexandra; Faustino, Augusto M. R.] Univ Porto, Biomed Sci Inst Abel Salazar, Dept Pathol & Mol Immunol, P-4050313 Oporto, Portugal
reprint address Bicho, RC (reprint author), Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal.
e-mail address ritabicho@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Science Foundation (FCT) [PEst-C/MAR/LA0017/2013, FUBIA FCOMP-01-0124-FEDER-008651 (PTDC/AAC-CLI/103719/2008), BI/UI88/4494/2012, SFRH/BD/102702/2014]
funding text This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013 and FUBIA FCOMP-01-0124-FEDER-008651 (Ref. PTDC/AAC-CLI/103719/2008) a research grant (Ref. BI/UI88/4494/2012) and a PhD grant to Rita Bicho (SFRH/BD/102702/2014).
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cited reference count 47
publisher PERGAMON-ELSEVIER SCIENCE LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
issn 0045-6535
29-character source abbreviation CHEMOSPHERE
iso source abbreviation Chemosphere
publication date NOV
year published 2015
volume 139
beginning page 386
ending page 392
digital object identifier (doi) 10.1016/j.chemosphere.2015.07.021
page count 7
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number CS1ZQ
unique article identifier WOS:000361868000051
link http://dx.doi.org/10.1016/j.chemosphere.2015.07.021