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title Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata
authors Escandon, M; Canal, MJ; Pascual, J; Pinto, G; Correia, B; Amaral, J; Meijon, M
author full name Escandon, Monica; Jesus Canal, Maria; Pascual, Jesus; Pinto, Gloria; Correia, Barbara; Amaral, Joana; Meijon, Monica
title Integrated physiological and hormonal profile of heat-induced thermotolerance in Pinus radiata
nationality internacional
source TREE PHYSIOLOGY
language English
document type Article
author keywords acclimation; conifers; ecophysiological parameters; heat tolerance; phytohormone crosstalk; UPLC/MS
keywords plus TANDEM MASS-SPECTROMETRY; SALICYLIC-ACID; ABSCISIC-ACID; WATER-STRESS; ACQUIRED THERMOTOLERANCE; PROLINE ACCUMULATION; OXIDATIVE DAMAGE; TREE MORTALITY; SHOCK PROTEINS; JASMONIC ACID
abstract Despite great interest, not only from the economic point of view but also in terms of basic science, research on heat stress tolerance in conifers remains scarce. To fill this gap, a time-course experiment using expected temperature increase was performed aiming to identify physiological and biochemical traits that allow the characterization of heat-induced thermotolerance and recovery in Pinus radiata D. Don plants. Several physiological parameters were assessed during heat exposure and after recovery, and multiple phytohormones-abscisic acid (ABA), indole-3-acetic acid (IAA), cytokinins (CKs), gibberellins, jasmonic acid, salicylic acid (SA) and brassinosteroids-were quantified by ultra-performance liquid chromatography-mass spectrometry from unique sample. Furthermore, tissue specific stress-signaling was monitored by IAA and ABA immunolocalization. Multivariate statistical analysis of the data enabled clustering of the shorter- and longer-term effects of heat stress exposure. Two sequential physiological responses were identified: an immediate and a delayed response, essentially determined by specific phytohormones, proline, malondialdehyde and total soluble sugar patterns. Results showed that ABA and SA play a crucial role in the first stage of response to heat stress, probably due to the plant's urgent need to regulate stomatal closure and counteract the increase in oxidative membrane damage demonstrated in shorter-term exposures. However, in longer exposures and recovery, proline, total sugars, IAA and CKs seem to be more relevant. This integrated approach pinpointed some basic mechanisms of P. radiata physiological responses underlying thermotolerance processes and after recovery.
author address [Escandon, Monica; Jesus Canal, Maria; Pascual, Jesus; Meijon, Monica] Univ Oviedo, Fac Biol, Dept BOS, Plant Physiol, E-33006 Oviedo, Asturias, Spain; [Pinto, Gloria; Correia, Barbara; Amaral, Joana] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Pinto, Gloria; Correia, Barbara; Amaral, Joana] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Meijon, Monica] Reg Inst Res & Agrofood Dev Asturias SERIDA, Expt Stn La Mata, Grado 33820, Asturias, Spain
reprint address Meijon, M (reprint author), Univ Oviedo, Fac Biol, Dept BOS, Plant Physiol, E-33006 Oviedo, Asturias, Spain.; Meijon, M (reprint author), Reg Inst Res & Agrofood Dev Asturias SERIDA, Expt Stn La Mata, Grado 33820, Asturias, Spain.
e-mail address mjcanal@uniovi.es
funding agency and grant number Spanish Ministry of Science and Innovation [AGL2011-27904]; FEDER through COMPETE [UID/AMB/50017/2013]; Severo Ochoa Programme (Government of Principado de Asturias, Spain) [BP11117]; Juan de la Cierva Programme (Spanish Ministry of Economy and Competitiviness) [JCI-2011-08958]; FPU (Ministry of Education, Spain) [AP2010-5857]; FCT (Fundacao para a Ciencia e a Tecnologia, Portugal) [SFRH/BD/86448/2012, SFRH/BPD/101669/2014]; [PTDC/AGR-FOR/2768/2014]
funding text This publication is an output of the Project financed by the Spanish Ministry of Science and Innovation (AGL2011-27904) and FEDER funding through COMPETE (project: UID/AMB/50017/2013). M.E. was supported by a fellowship from the Severo Ochoa Programme (BP11117) (Government of Principado de Asturias, Spain). M.M. is funded by the Juan de la Cierva Programme (JCI-2011-08958) (Spanish Ministry of Economy and Competitiviness). J.P. was supported by a fellowship from the FPU (AP2010-5857) (Ministry of Education, Spain). FCT (Fundacao para a Ciencia e a Tecnologia, Portugal) supported fellowships of B.C. (SFRH/BD/86448/2012) and G.P. (SFRH/BPD/101669/2014) and the project (PTDC/AGR-FOR/2768/2014).
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cited reference count 65
publisher OXFORD UNIV PRESS
publisher city OXFORD
publisher address GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
issn 0829-318X
29-character source abbreviation TREE PHYSIOL
iso source abbreviation Tree Physiol.
publication date JAN
year published 2016
volume 36
issue 1
beginning page 63
ending page 77
digital object identifier (doi) 10.1093/treephys/tpv127
page count 15
web of science category Forestry
subject category Forestry
document delivery number DC6LU
unique article identifier WOS:000369332300006