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title Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit
authors Jesus, C; Meijon, M; Monteiro, P; Correia, B; Amaral, J; Escandon, M; Canal, MJ; Pinto, G
title Salicylic acid application modulates physiological and hormonal changes in Eucalyptus globulus under water deficit
nationality internacional
source ENVIRONMENTAL AND EXPERIMENTAL BOTANY
language English
document type Article
author keywords Water deficit; Plant performance; Phytohormones crosstalk; Priming; Forest trees
keywords plus TANDEM MASS-SPECTROMETRY; LIPID-PEROXIDATION; DROUGHT TOLERANCE; EXOGENOUS APPLICATION; WHEAT SEEDLINGS; STRESS; GROWTH; RESPONSES; PLANTS; RECOVERY
abstract Eucalyptus genus is the most widely planted hardwood tree, which productivity and development are limited by low water availability. Plant drought tolerance can be managed by adopting strategies, such as the exogenous application of salicylic acid. The main objective of the present study was to assess whether the exogenous foliar salicylic acid application would ameliorate the damages of water deficit on Eucalyptus globulus plants. Plants were watered at 70% (well water) or 15% (water deficit) of field capacity and four concentrations of salicylic acid (0, 0.75, 2.5 and 5.0 mM) were applied. Water potential, total chlorophylls and carotenoids contents, chlorophyll fluorescence parameters, leaf gas exchange, malondialdehyde, total soluble sugars, starch and total phenols contents were measured. The global hormonal content was quantified by ultra-performance liquid chromatography-mass spectrometry and specific local dynamics of indolacetic acid and abcisic acid were detected by immunolocalization in leaves. A multivariate statistical approach was used to get an overview of the plant physiological status. E. globulus water deficit response included growth rate decline associated with reduced in both water potential and leaf gas exchange parameters. Plant water deficit defence strategies led to an increase in total chlorophylls and carotenoids contents, lipid peroxidation, phenols and total soluble sugars. Six from the 18 hormones detected increased in water deficit plants. Exogenous salicylic acid application improved water deficit tolerance of E. globulus by improving water potential with a positive impact in primary metabolism (photosynthetic rate, soluble sugars) but also in secondary metabolism and defence mechanisms (higher total phenols and less lipid peroxidation) in the highest salicylic acid concentrations. Also, changes in endogenous levels of abscisic and salicylic acids, gibberellins 4 and 7, and specific cytokinins were found in water deficit plants with salicylic acid application. Our results indicated that salicylic acid application could be a potential chemical priming strategy to ameliorate water deficit effects on E. globulus plants. (C) 2015 Elsevier B.V. All rights reserved.
author address [Jesus, Claudia; Monteiro, Pedro; Correia, Barbara; Amaral, Joana; Pinto, Gloria] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Jesus, Claudia; Monteiro, Pedro; Correia, Barbara; Amaral, Joana; Pinto, Gloria] Univ Aveiro, CESAM Ctr Environm & Marine Studies, P-3810193 Aveiro, Portugal; [Meijon, Monica] Reg Inst Res & Agrofood Dev Asturias SERIDA, La Mata Expt Stn, Grado 33820, Asturias, Spain; [Escandon, Monica; Canal, Maria Jesus] Univ Oviedo, Dept BOS, Plant Physiol, E-33071 Oviedo, Asturias, Spain
reprint address Pinto, G (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address mmeijon@serida.org; gpinto@ua.pt
researcherid number Pinto, GP/B-1271-2011; Correia, Barbara/I-2146-2014; CESAM, UA/M-3762-2015; Canal, Maria Jesus/L-1005-2014; Meijon, Monica/G-7366-2016; Meijon, Monica/N-6285-2014;
orcid number Pinto, GP/0000-0001-7735-5131; Correia, Barbara/0000-0002-8277-7061; Canal, Maria Jesus/0000-0002-1639-9672; Meijon, Monica/0000-0003-1563-5554; Meijon, Monica/0000-0003-1563-5554; Jesus, Claudia/0000-0002-4422-7616
funding agency and grant number FEDER through COMPETE (Programa Operacional Fatores de Competitividade); FCT [PTDC/AGR-CFL/112996/2009, SFRH/BD/86448/2012, SFRH/BPD/101669/2014]; Spanish Ministry of Economy and Competitiviness [JCI-2011-08958]; Gobierno del Principado de Asturias, Spain [BP11117]; [UID/AMB/50017/2013]
funding text The authors extend thanks to Armando Costa (University of Aveiro, Portugal) for help with experimental set up, Luis Valledor (University of Oviedo) for statistical advance, Carolina de la Torre and Isabel Feito (SERIDA, Spain) for their assistant support in phytohormone extraction. This research was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade), by the FCT project PTDC/AGR-CFL/112996/2009 and by UID/AMB/50017/2013. FCT supported the fellowship of B Correia (SFRH/BD/86448/2012) and G Pinto (SFRH/BPD/101669/2014). M Meijon is funded by Juan de la Cierva Programme (JCI-2011-08958) (Spanish Ministry of Economy and Competitiviness) and M. Escandon fellowship was supported by Severo Ochoa Programme (BP11117) (Gobierno del Principado de Asturias, Spain).
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cited reference count 68
times cited 2
total times cited count (wos, bci, and cscd) 2
publisher PERGAMON-ELSEVIER SCIENCE LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
issn 0098-8472
29-character source abbreviation ENVIRON EXP BOT
iso source abbreviation Environ. Exp. Bot.
publication date OCT
year published 2015
volume 118
beginning page 56
ending page 66
digital object identifier (doi) 10.1016/j.envexpbot.2015.06.004
page count 11
web of science category Plant Sciences; Environmental Sciences
subject category Plant Sciences; Environmental Sciences & Ecology
document delivery number CO3AL
unique article identifier WOS:000359028500007
CESAM authors