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title The contribution of Escherichia coli from human and animal sources to the integron gene pool in coastal waters
authors Moura, A; Araujo, S; Alves, MS; Henriques, I; Pereira, A; Correia, ACM
author full name Moura, Alexandra; Araujo, Susana; Alves, Marta S.; Henriques, Isabel; Pereira, Anabela; Correia, Antonio C. M.
title The contribution of Escherichia coli from human and animal sources to the integron gene pool in coastal waters
nationality internacional
source FRONTIERS IN MICROBIOLOGY
language English
document type Article
author keywords environmental reservoirs; microbial risk assessment; multi-resistance; integron diversity; replicon typing; Enterobacteriaceae
keywords plus ANTIBIOTIC-RESISTANCE GENES; SPECTRUM BETA-LACTAMASES; BLACK-HEADED GULLS; ANTIMICROBIAL RESISTANCE; CLASS-1 INTEGRONS; AQUATIC SYSTEMS; WILD BIRDS; SEA-GULLS; SALMONELLA; CASSETTES
abstract To understand the contribution of animal- and human-derived fecal pollution sources in shaping integron prevalence and diversity in beach waters, 414 Escherichia coli strains were collected from beach waters (BW, n = 166), seagull feces (SF n = 179), and wastewaters (WW, n = 69), on the World Biosphere Reserve of the Berlenga Island, Portugal. Statistical differences were found between the prevalence of integrons in BW (21%) and WW (10%), but not between BW and SF (19%). The majority of integrase-positive (int/(+))-strains affiliated to commensal phylogroups B1 (37%), A0 (24%), and A1 (20%). Eighteen different gene cassette arrays were detected, most of them coding for resistances to aminoglycosides, trimethoprim, chloramphenicol, and quaternary ammonia compounds. Common arrays were found among strains from different sources. Multi-resistance to three or more different classes of antibiotics was observed in 89, 82, and 57% of int/(+)-strains from BW, SF and WW, respectively. Plasmids were detected in 79% of strains (60/76) revealing a high diversity of replicons in all sources, mostly belonging to IncF (Frep, FIA, and FIB subgroups), Incl1, IncN, IncY, and IncK incompatibility groups. In 20% (15/76) of strains, integrons were successfully mobilized through conjugation to E. coli CV601. Results obtained support the existence of a diverse integron pool in the E. coli strains from this coastal environment, associated with different resistance traits and plasmid incompatibility groups, mainly shaped by animal fecal pollution inputs. These findings underscore the role of wild life in dissemination of integrons and antibiotic resistance traits in natural environments.
author address [Moura, Alexandra] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Moura, Alexandra] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
reprint address Moura, A (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address amoura@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through FCT-Fundacao para a Ciencia e a Tecnologia [PEst-C/MAR/LA0017/2013]; SEAGULL [FCOMP-01-0124-FEDER-008640), PTDC/AAC-AMB/109155/2008]; FCT [SFRH/BPD/72256/2010, SFRH/BPD/26685/2006]; project Sustainable Use of Marine Resources MARES [CENTRO-07-ST24-FEDER-002033]; QREN (Mais Centro-Programa Operacional Regional do Centro); FEDER
funding text The authors wish to thank Alessandra Carattoli (Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita, Italy) for providing replicon typing control strains. This work was supported by European Funds through COMPETE and by National Funds through FCT-Fundacao para a Ciencia e a Tecnologia within the projects PEst-C/MAR/LA0017/2013 and SEAGULL (FCOMP-01-0124-FEDER-008640, PTDC/AAC-AMB/109155/2008). FCT also financed the grants SFRH/BPD/72256/2010 (Alexandra Moura) and SFRH/BPD/26685/2006 (Anabela Pereira). Isabel Henriques received an individual Research Assistant contract within the project Sustainable Use of Marine Resources MARES (CENTRO-07-ST24-FEDER-002033), co-financed by QREN (Mais Centro-Programa Operacional Regional do Centro) and FEDER.
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cited reference count 52
publisher FRONTIERS RESEARCH FOUNDATION
publisher city LAUSANNE
publisher address PO BOX 110, LAUSANNE, 1015, SWITZERLAND
issn 1664-302X
29-character source abbreviation FRONT MICROBIOL
iso source abbreviation Front. Microbiol.
publication date AUG 12
year published 2014
volume 5
article number 419
digital object identifier (doi) 10.3389/fmicb.2014.00419
page count 15
web of science category Microbiology
subject category Microbiology
document delivery number AO7GK
unique article identifier WOS:000341520300001
link http://dx.doi.org/10.3389/fmicb.2014.00419