HOME  »  Members  »  Collaborators  »  PhD Students/Fellows
title The contribution of Escherichia coli from human and animal sources to the integron gene pool in coastal waters
authors Moura, A; Araujo, S; Alves, MS; Henriques, I; Pereira, A; Correia, ACM
author full name Moura, Alexandra; Araujo, Susana; Alves, Marta S.; Henriques, Isabel; Pereira, Anabela; Correia, Antonio C. M.
title The contribution of Escherichia coli from human and animal sources to the integron gene pool in coastal waters
nationality internacional
language English
document type Article
author keywords environmental reservoirs; microbial risk assessment; multi-resistance; integron diversity; replicon typing; Enterobacteriaceae
abstract To understand the contribution of animal- and human-derived fecal pollution sources in shaping integron prevalence and diversity in beach waters, 414 Escherichia coli strains were collected from beach waters (BW, n = 166), seagull feces (SF n = 179), and wastewaters (WW, n = 69), on the World Biosphere Reserve of the Berlenga Island, Portugal. Statistical differences were found between the prevalence of integrons in BW (21%) and WW (10%), but not between BW and SF (19%). The majority of integrase-positive (int/(+))-strains affiliated to commensal phylogroups B1 (37%), A0 (24%), and A1 (20%). Eighteen different gene cassette arrays were detected, most of them coding for resistances to aminoglycosides, trimethoprim, chloramphenicol, and quaternary ammonia compounds. Common arrays were found among strains from different sources. Multi-resistance to three or more different classes of antibiotics was observed in 89, 82, and 57% of int/(+)-strains from BW, SF and WW, respectively. Plasmids were detected in 79% of strains (60/76) revealing a high diversity of replicons in all sources, mostly belonging to IncF (Frep, FIA, and FIB subgroups), Incl1, IncN, IncY, and IncK incompatibility groups. In 20% (15/76) of strains, integrons were successfully mobilized through conjugation to E. coli CV601. Results obtained support the existence of a diverse integron pool in the E. coli strains from this coastal environment, associated with different resistance traits and plasmid incompatibility groups, mainly shaped by animal fecal pollution inputs. These findings underscore the role of wild life in dissemination of integrons and antibiotic resistance traits in natural environments.
author address [Moura, Alexandra] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Moura, Alexandra] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
reprint address Moura, A (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address amoura@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through FCT-Fundacao para a Ciencia e a Tecnologia [PEst-C/MAR/LA0017/2013]; SEAGULL [FCOMP-01-0124-FEDER-008640), PTDC/AAC-AMB/109155/2008]; FCT [SFRH/BPD/72256/2010, SFRH/BPD/26685/2006]; project Sustainable Use of Marine Resources MARES [CENTRO-07-ST24-FEDER-002033]; QREN (Mais Centro-Programa Operacional Regional do Centro); FEDER
funding text The authors wish to thank Alessandra Carattoli (Department of Infectious, Parasitic and Immune-Mediated Diseases, Istituto Superiore di Sanita, Italy) for providing replicon typing control strains. This work was supported by European Funds through COMPETE and by National Funds through FCT-Fundacao para a Ciencia e a Tecnologia within the projects PEst-C/MAR/LA0017/2013 and SEAGULL (FCOMP-01-0124-FEDER-008640, PTDC/AAC-AMB/109155/2008). FCT also financed the grants SFRH/BPD/72256/2010 (Alexandra Moura) and SFRH/BPD/26685/2006 (Anabela Pereira). Isabel Henriques received an individual Research Assistant contract within the project Sustainable Use of Marine Resources MARES (CENTRO-07-ST24-FEDER-002033), co-financed by QREN (Mais Centro-Programa Operacional Regional do Centro) and FEDER.
cited references Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Alves MS, 2014, FRONT MICROBIOL, V5, DOI 10.3389/fmicb.2014.00426; Andersson DI, 2011, FEMS MICROBIOL REV, V35, P901, DOI 10.1111/j.1574-6976.2011.00289.x; Antunes P, 2007, ANTIMICROB AGENTS CH, V51, P1545, DOI 10.1128/AAC.01275-06; Araujo S, 2014, SCI TOTAL ENVIRON, V470, P84, DOI 10.1016/j.scitotenv.2013.09.075; Arduino SM, 2003, ANTIMICROB AGENTS CH, V47, P3945, DOI 10.1128/AAC.47.12.3945-3949.2003; Baharoglu Z, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1001165; Carattoli A, 2005, J MICROBIOL METH, V63, P219, DOI 10.1016/j.mimet.2005.03.018; Carattoli A, 2013, INT J MED MICROBIOL, V303, P298, DOI 10.1016/j.ijmm.2013.02.001; Clermont O, 2000, APPL ENVIRON MICROB, V66, P4555, DOI 10.1128/AEM.66.10.4555-4558.2000; Clinical and Laboratory Standards Institutes (CLSI), 2012, M100S22 CLSI; Curiao T, 2011, ANTIMICROB AGENTS CH, V55, P2451, DOI 10.1128/AAC.01448-10; Davies J, 2010, MICROBIOL MOL BIOL R, V74, P417, DOI 10.1128/MMBR.00016-10; Dolejska M, 2007, J APPL MICROBIOL, V103, P11, DOI 10.1111/j.1365-2672.2006.03241.x; Dolejska M, 2009, J APPL MICROBIOL, V106, P1941, DOI 10.1111/j.1365-2672.2009.04155.x; Figueira V, 2011, SCI TOTAL ENVIRON, V409, P1017, DOI 10.1016/j.scitotenv.2010.12.011; Goldstein C, 2001, ANTIMICROB AGENTS CH, V45, P723, DOI 10.1128/AAC.45.3.723-726.2001; Guentzel M. N, 1996, MED MICROBIOLOGY; Hernandez J, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0076150; Heuer H, 2007, ENVIRON MICROBIOL, V9, P657, DOI 10.1111/j.1462-2920.2006.01185.x; Jove T, 2010, PLOS GENET, V6, DOI 10.1371/journal.pgen.1000793; Kmet V, 2013, ANN AGR ENV MED, V20, P273; KRAFT CA, 1986, J MED MICROBIOL, V22, P125; LEVESQUE C, 1995, ANTIMICROB AGENTS CH, V39, P185; Moura A, 2012, FEMS MICROBIOL LETT, V330, P157, DOI 10.1111/j.1574-6968.2012.02544.x; Moura A, 2009, BIOINFORMATICS, V25, P1096, DOI 10.1093/bioinformatics/btp105; Moura A, 2007, J ANTIMICROB CHEMOTH, V60, P1243, DOI 10.1093/jac/dkm340; Moura A, 2012, RES MICROBIOL, V163, P92, DOI 10.1016/j.resmic.2011.10.010; Moura A, 2012, APPL ENVIRON MICROB, V78, P5413, DOI 10.1128/AEM.00042-12; Perez-Moreno MO, 2013, INT J MED MICROBIOL, V303, P25, DOI 10.1016/j.ijmm.2012.11.003; Perry JA, 2013, FRONT MICROBIOL, V4, DOI 10.3389/fmicb.2013.00138; Poeta P, 2008, APPL ENVIRON MICROB, V74, P7439, DOI 10.1128/AEM.00949-08; Poirel L, 2012, ANTIMICROB AGENTS CH, V56, P2756, DOI 10.1128/AAC.05982-11; Post V, 2007, ANTIMICROB AGENTS CH, V51, P3467, DOI 10.1128/AAC.00220-07; Radhouani H, 2009, VET REC, V165, P138; Randall LP, 2004, J ANTIMICROB CHEMOTH, V53, P208, DOI 10.1093/jac/dkh070; Saenz Y, 2010, VET MICROBIOL, V144, P493, DOI 10.1016/j.vetmic.2010.01.026; Sandvang D, 1997, FEMS MICROBIOL LETT, V157, P177, DOI 10.1016/S0378-1097(97)00473-4; Santos T, 2013, ANAEROBE, V24, P25, DOI 10.1016/j.anaerobe.2013.09.004; Siguier P, 2006, NUCLEIC ACIDS RES, V34, pD32, DOI 10.1093/nar/gkj014; Smalla K, 2006, APPL ENVIRON MICROB, V72, P7253, DOI 10.1128/AEM.00922-06; Soufi L, 2009, FOODBORNE PATHOG DIS, V6, P1067, DOI 10.1089/fpd.2009.0284; STOKES HW, 1989, MOL MICROBIOL, V3, P1669, DOI 10.1111/j.1365-2958.1989.tb00153.x; Tacao M, 2014, WATER RES, V48, P100, DOI 10.1016/j.watres.2013.09.021; Tacao M, 2013, ANTIMICROB AGENTS CH, V57, P6399, DOI 10.1128/AAC.00771-13; Tacao M, 2012, APPL ENVIRON MICROB, V78, P4134, DOI 10.1128/AEM.00359-12; Tenaillon O, 2010, NAT REV MICROBIOL, V8, P207, DOI 10.1038/nrmicro2298; Touchon M, 2009, PLOS GENET, V5, DOI 10.1371/journal.pgen.1000344; van Elsas JD, 2011, ISME J, V5, P173, DOI 10.1038/ismej.2010.80; Veldman K, 2013, APPL ENVIRON MICROB, V79, P7556, DOI 10.1128/AEM.01880-13; VERSALOVIC J, 1991, NUCLEIC ACIDS RES, V19, P6823, DOI 10.1093/nar/19.24.6823; White PA, 2001, ANTIMICROB AGENTS CH, V45, P2658, DOI 10.1128/AAC.45.9.2658-2661.2001
cited reference count 52
publisher city LAUSANNE
publisher address PO BOX 110, LAUSANNE, 1015, SWITZERLAND
issn 1664-302X
29-character source abbreviation FRONT MICROBIOL
iso source abbreviation Front. Microbiol.
publication date AUG 12
year published 2014
volume 5
article number 419
digital object identifier (doi) 10.3389/fmicb.2014.00419
page count 15
web of science category Microbiology
subject category Microbiology
document delivery number AO7GK
unique article identifier WOS:000341520300001
link http://dx.doi.org/10.3389/fmicb.2014.00419