HOME  »  Members  »  Collaborators  »  PhD Students/Fellows
 
title Seawater is a reservoir of mult-resistant Escherichia coli, including strains hosting plasmid-mediated quinolones resistance and extended-spectrum beta-lactarnases genes
authors Alves, MS; Pereira, A; Araujo, SM; Castro, BB; Correia, ACM; Henriques, I
author full name Alves, Marta S.; Pereira, Anabela; Araujo, Susana M.; Castro, Bruno B.; Correia, Antonio C. M.; Henriques, Isabel
title Seawater is a reservoir of mult-resistant Escherichia coli, including strains hosting plasmid-mediated quinolones resistance and extended-spectrum beta-lactarnases genes
nationality internacional
source FRONTIERS IN MICROBIOLOGY
language English
document type Article
author keywords Escherichia coli; antibiotic resistance; fecal pollution; water quality; Microbial source tracking
keywords plus ANTIBIOTIC-RESISTANCE; ANTIMICROBIAL RESISTANCE; DRINKING-WATER; AQUATIC SYSTEMS; SOURCE TRACKING; LACTAMASE; DIVERSITY; BACTERIA; ENTEROBACTERIACEAE; INTEGRONS
abstract The aim of this study was to examine antibiotic resistance (AR) dissemination in coastal water, considering the contribution of different sources of fecal contamination. Samples were collected in Berlenga, an uninhabited island classified as Natural Reserve and visited by tourists for aquatic recreational activities. To achieve our aim, AR in Escherichia colt isolates from coastal water was compared to AR in isolates from two sources of fecal contamination: human-derived sewage and seagull feces. Isolation of E. colt was done on Chromocult agar. Based on genetic typing 414 strains were established. Distribution of E. colt phylogenetic groups was similar among isolates of all sources. Resistances to streptomycin, tetracycline, cephalothin, and amoxicillin were the most frequent. Higher rates of AR were found among seawater and feces isolates, except for last-line antibiotics used in human medicine. Multi-resistance rates in isolates from sewage and seagull feces (29 and 32%) were lower than in isolates from seawater (39%). Seawater AR profiles were similar to those from seagull feces and differed significantly from sewage AR profiles. Nucleotide sequences matching resistance genes bla(TEM), sun, sul2, tet(A), and tet(B), were present in isolates of all sources. Genes conferring resistance to 3rd generation cephalosporins were detected in seawater (bla(CTX-M-1) and bla(SHV-12)) and seagull feces (bla(CMY-2)). Plasmid-mediated determinants of resistance to quinolones were found: qnrS1 in all sources and qnrB19 in seawater and seagull feces. Our results show that seawater is a relevant reservoir of AR and that seagulls are an efficient vehicle to spread human-associated bacteria and resistance genes. The E. colt resistome recaptured from Berlenga coastal water was mainly modulated by seagulls-derived fecal pollution. The repertoire of resistance genes covers antibiotics critically important for humans, a potential risk for human health.
author address [Henriques, Isabel] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Henriques, Isabel] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
reprint address Henriques, I (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address ihenriques@ua.pt
researcherid number Correia, Antonio/B-1593-2008; Pereira, Anabela/H-4800-2013; Araujo, Susana/F-8290-2013
orcid number Correia, Antonio/0000-0002-5115-1429; Pereira, Anabela/0000-0003-2351-1084;
funding agency and grant number Portuguese Foundation for Science and Technology (FCT) [PTDC/AAC-AMB/109155/2008, FCOMP-01-0124-FEDER-008640]; . FCT [SFRH/BPD/26685/2006]; project MARES - National Strategic reference Framework [SCT_2011_02_033_4904]; Programme Ciencia; Human Potential Operational Programme (National Strategic Reference Framework) [20072013]; European Social Fund (EU); European Funds through COMPETE; National Funds through the Portuguese Science Foundation (FCT) [PEst-C/MAR/LA0017/2013]
funding text The Portuguese Foundation for Science and Technology (FCT) supported this study through projects PTDC/AAC-AMB/109155/2008 and FCOMP-01-0124-FEDER-008640. FCT also financed the fellowships of A Pereira (SFRH/BPD/26685/2006). I Henriques was supported by the project MARES (Sustainable Use of Marine Resources; ref. SCT_2011_02_033_4904) funded by National Strategic reference Framework. Bruno B. Castro was supported by the Programme Ciencia 2008, co-funded by the Human Potential Operational Programme (National Strategic Reference Framework 20072013) and European Social Fund (EU). This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013.
cited references Alouache S, 2012, MICROBES ENVIRON, V27, P80, DOI 10.1264/jsme2.ME11266; Aminov RI, 2011, FRONT MICROBIOL, V2, DOI 10.3389/fmicb.2011.00158; Araujo S, 2014, SCI TOTAL ENVIRON, V470, P84, DOI 10.1016/j.scitotenv.2013.09.075; Austin DJ, 1999, P NATL ACAD SCI USA, V96, P1152, DOI 10.1073/pnas.96.3.1152; Baquero F, 2008, CURR OPIN BIOTECH, V19, P260, DOI 10.1016/j.copbio.2008.05.006; Bonnedahl J, 2009, PLOS ONE, V4, DOI 10.1371/journal.pone.0005958; Canton R, 2006, CURR OPIN MICROBIOL, V9, P466, DOI 10.1016/j.mib.2006.08.011; Carroll SP, 2009, WATER RES, V43, P1237, DOI 10.1016/j.watres.2008.12.017; Cattoir V, 2007, ANTIMICROB AGENTS CH, V51, P2652, DOI 10.1128/AAC.01616-06; Clermont O, 2000, APPL ENVIRON MICROB, V66, P4555, DOI 10.1128/AEM.66.10.4555-4558.2000; Clinical and Laboratory Standards Institute (CLSI), 2012, M100S22 CLSI; Dallenne C, 2010, J ANTIMICROB CHEMOTH, V65, P490, DOI 10.1093/jac/dkp498; Devirgiliis C, 2011, GENES NUTR, V6, P275, DOI 10.1007/s12263-011-0226-x; Dolejska M, 2009, J APPL MICROBIOL, V106, P1941, DOI 10.1111/j.1365-2672.2009.04155.x; Dolejska M, 2013, J ANTIMICROB CHEMOTH, V68, P333, DOI 10.1093/jac/dks387; Eckert C, 2006, J ANTIMICROB CHEMOTH, V57, P14, DOI 10.1093/jac/dki398; European Centre for Disease Prevention and Control (ECDC), 2013, ANN REP EUR ANT RES; Field KG, 2007, WATER RES, V41, P3517, DOI 10.1016/j.watres.2007.06.056; Figueira V, 2011, SCI TOTAL ENVIRON, V409, P1017, DOI 10.1016/j.scitotenv.2010.12.011; Freitas F, 2014, EUR J CLIN MICROBIOL, V33, P551, DOI 10.1007/s10096-013-1983-9; Gionechetti F, 2008, MICROB DRUG RESIST, V14, P93, DOI 10.1089/mdr.2008.0803; Gomez-Alvarez V, 2012, APPL ENVIRON MICROB, V78, P6095, DOI 10.1128/AEM.01018-12; Guillard T, 2011, DIAGN MICR INFEC DIS, V70, P253, DOI 10.1016/j.diagmicrobio.2011.01.004; Ham YS, 2012, ENVIRON POLLUT, V162, P98, DOI 10.1016/j.envpol.2011.11.002; Harwood VJ, 2014, FEMS MICROBIOL REV, V38, P1, DOI 10.1111/1574-6976.12031; Henriques I, 2006, FEMS MICROBIOL ECOL, V56, P418, DOI 10.1111/j.1574-6941.2006.00073.x; Henriques IS, 2006, RES MICROBIOL, V157, P938, DOI 10.1016/j.resmic.2006.09.003; Henriques IS, 2012, MICROB DRUG RESIST, V18, P531, DOI 10.1089/mdr.2012.0029; Heuer H, 2007, ENVIRON MICROBIOL, V9, P657, DOI 10.1111/j.1462-2920.2006.01185.x; Johnson JR, 2001, J INFECT DIS, V183, P78, DOI 10.1086/317656; Kang MS, 2006, ANTIMICROB AGENTS CH, V50, P1590, DOI 10.1128/AAC.50.4.1590-1593.2006; Kaper JB, 2004, NAT REV MICROBIOL, V2, P123, DOI 10.1038/nrmicro818; Literak I, 2010, APPL ENVIRON MICROB, V76, P8126, DOI 10.1128/AEM.01446-10; Lu SY, 2010, APPL ENVIRON MICROB, V76, P5972, DOI 10.1128/AEM.00711-10; Mataseje LF, 2009, ANTIMICROB AGENTS CH, V53, P3126, DOI 10.1128/AAC.01353-08; Nawaz M, 2006, APPL ENVIRON MICROB, V72, P6461, DOI 10.1128/AEM.00271-06; Papp-Wallace KM, 2011, ANTIMICROB AGENTS CH, V55, P4943, DOI 10.1128/AAC.00296-11; Parveen S, 1997, APPL ENVIRON MICROB, V63, P2607; Pereira A, 2013, SCI TOTAL ENVIRON, V461, P65, DOI 10.1016/j.scitotenv.2013.04.067; Picard B, 1999, INFECT IMMUN, V67, P546; Poirel L, 2012, ANTIMICROB AGENTS CH, V56, P2756, DOI 10.1128/AAC.05982-11; Poirel L, 2004, ANTIMICROB AGENTS CH, V48, P15, DOI 10.1128/AAC.48.1.15-22.2004; Radhouani H, 2009, VET REC, V165, P138; Rosewarne CP, 2010, FEMS MICROBIOL ECOL, V72, P35, DOI 10.1111/j.1574-6941.2009.00823.x; Saladin M, 2002, FEMS MICROBIOL LETT, V209, P161, DOI 10.1111/j.1574-6968.2002.tb11126.x; Simoes RR, 2010, EMERG INFECT DIS, V16, P110, DOI 10.3201/eid1601.090896; Speldooren V, 1998, ANTIMICROB AGENTS CH, V42, P879; Su HC, 2012, ENVIRON POLLUT, V169, P42, DOI 10.1016/j.envpol.2012.05.007; Tacao M, 2014, WATER RES, V48, P100, DOI 10.1016/j.watres.2013.09.021; Tacao M, 2012, APPL ENVIRON MICROB, V78, P4134, DOI 10.1128/AEM.00359-12; van Elsas JD, 2011, ISME J, V5, P173, DOI 10.1038/ismej.2010.80; Verdet C, 2009, ANTIMICROB AGENTS CH, V53, P4002, DOI 10.1128/AAC.00753-08; Vredenburg J, 2014, ENVIRON MICROBIOL, V16, P995, DOI 10.1111/1462-2920.12231; Wellington EMH, 2013, LANCET INFECT DIS, V13, P155, DOI 10.1016/S1473-3099(12)70317-1; Wicki M, 2011, APPL ENVIRON MICROB, V77, P8427, DOI 10.1128/AEM.05651-11; Zhao JY, 2012, MICROB ECOL, V64, P187, DOI 10.1007/s00248-012-0008-z
cited reference count 56
times cited 2
total times cited count (wos, bci, and cscd) 2
publisher FRONTIERS RESEARCH FOUNDATION
publisher city LAUSANNE
publisher address PO BOX 110, LAUSANNE, 1015, SWITZERLAND
issn 1664-302X
29-character source abbreviation FRONT MICROBIOL
iso source abbreviation Front. Microbiol.
publication date AUG 20
year published 2014
volume 5
article number 426
digital object identifier (doi) 10.3389/fmicb.2014.00426
page count 10
web of science category Microbiology
subject category Microbiology
document delivery number AO7VJ
unique article identifier WOS:000341560400002
link http://dx.doi.org/10.3389/fmicb.2014.00426