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title Expansion of lugworms towards southern European habitats and their identification using combined ecological, morphological and genetic approaches
authors Pires, A; Martins, R; Magalhaes, L; Soares, AMVM; Figueira, E; Quintino, V; Rodrigues, AM; Freitas, R
author full name Pires, A.; Martins, R.; Magalhaes, L.; Soares, A. M. V. M.; Figueira, E.; Quintino, V.; Rodrigues, A. M.; Freitas, R.
title Expansion of lugworms towards southern European habitats and their identification using combined ecological, morphological and genetic approaches
nationality internacional
language English
document type Article
author keywords Arenicola defodiens; Arenicola marina; Iberian Peninsula; Arenicolidae; 16S; COI
abstract This study presents the first record of the recently colonizing lugworm species Arenicola defodiens in the Iberian Peninsula, and both A. defodiens and Arenicola marina in the Ria de Aveiro lagoon (northwest Portugal). We aimed firstly to provide a distinction between these 2 species, using combined morphological and genetic approaches, and secondly to determine their spatial distribution in the lagoon. Morphological characteristics such as annulation pattern, gill morphology, and faecal cast size enabled differentiation between both species, and we provide an updated taxonomic key for the valid species of the Family Arenicolidae known in the Iberian Peninsula. To confirm the distinction between A. defodiens and A. marina, 16S rDNA and COI gene sequencing analyses were performed. The percentage of nucleotide divergence between A. defodiens and A. marina was 13.5% for COI and 5% for 16S, and phylogenetic analyses showed that these are closely related species. Recent surveys conducted in Ria de Aveiro lagoon indicate that both species are dispersing throughout the system. Lugworm faecal casts were observed for the first time at 4 sites in 2009, and then at 7 sites in 2011. In 2014, A. marina was recorded at 2 intertidal sites of the Ria de Aveiro, which were characterized by very fine and medium sand. A. defodiens displayed a wider distribution, being reported at 13 intertidal and 5 subtidal sites where sediment characteristics ranged from mud to medium sand. A. marina densities ranged from 20.5 to 26.0 ind. m(2), higher than those recorded for A. defodiens, which did not exceed 3 ind. m(2). The expansion and the establishment of arenicolids in the Ria de Aveiro lagoon could be a result of the 2009-2010 and 2010-2011 cold winters. Possible consequences of the spread of these bioturbators in this lagunar ecosystem are discussed.
author address [Pires, A.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
reprint address Pires, A (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address adilia@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Science Foundation (FCT) [PEst-C/MAR/LA0017/2013]; Portuguese FCT [PD/00114/2012]; CESAM research funds [BPD/CESAM/RP/BENTONICAS/2013]; National Funds through the Post-doctoral grants [SFRH/BPD/93225/2013, SFRH/BPD/92258/2013]
funding text This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013 and through the Post-doctoral grants to R.M. (SFRH/BPD/93225/2013) and R.F. (SFRH/BPD/92258/2013). L.M. benefited from a PhD grant (PD/00114/2012) given by the Portuguese FCT. A.P. also benefitted from a Post-doctoral grant (BPD/CESAM/RP/BENTONICAS/2013), funded by CESAM research funds. The authors thank Aldiro Pereira and Rui Marques for support during sampling campaigns, Anthony Peter Moreira for the English editing, and Stefania Chiesa for help with genetic analysis.
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cited reference count 77
publisher city OLDENDORF LUHE
publisher address NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY
issn 0171-8630
29-character source abbreviation MAR ECOL PROG SER
iso source abbreviation Mar. Ecol.-Prog. Ser.
publication date AUG 6
year published 2015
volume 533
beginning page 177
ending page 190
digital object identifier (doi) 10.3354/meps11315
page count 14
web of science category Ecology; Marine & Freshwater Biology; Oceanography
subject category Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography
document delivery number CO7ZE
unique article identifier WOS:000359382300013
link http://dx.doi.org/10.3354/meps11315