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title Expansion of lugworms towards southern European habitats and their identification using combined ecological, morphological and genetic approaches
authors Pires, A; Martins, R; Magalhaes, L; Soares, AMVM; Figueira, E; Quintino, V; Rodrigues, AM; Freitas, R
author full name Pires, A.; Martins, R.; Magalhaes, L.; Soares, A. M. V. M.; Figueira, E.; Quintino, V.; Rodrigues, A. M.; Freitas, R.
title Expansion of lugworms towards southern European habitats and their identification using combined ecological, morphological and genetic approaches
nationality internacional
source MARINE ECOLOGY PROGRESS SERIES
language English
document type Article
author keywords Arenicola defodiens; Arenicola marina; Iberian Peninsula; Arenicolidae; 16S; COI
keywords plus ARENICOLA-MARINA; REPRODUCTIVE-BIOLOGY; MOLECULAR PHYLOGENY; CONTINENTAL-SHELF; TIDAL FLATS; WADDEN SEA; POLYCHAETA; AVEIRO; PORTUGAL; RIA
abstract This study presents the first record of the recently colonizing lugworm species Arenicola defodiens in the Iberian Peninsula, and both A. defodiens and Arenicola marina in the Ria de Aveiro lagoon (northwest Portugal). We aimed firstly to provide a distinction between these 2 species, using combined morphological and genetic approaches, and secondly to determine their spatial distribution in the lagoon. Morphological characteristics such as annulation pattern, gill morphology, and faecal cast size enabled differentiation between both species, and we provide an updated taxonomic key for the valid species of the Family Arenicolidae known in the Iberian Peninsula. To confirm the distinction between A. defodiens and A. marina, 16S rDNA and COI gene sequencing analyses were performed. The percentage of nucleotide divergence between A. defodiens and A. marina was 13.5% for COI and 5% for 16S, and phylogenetic analyses showed that these are closely related species. Recent surveys conducted in Ria de Aveiro lagoon indicate that both species are dispersing throughout the system. Lugworm faecal casts were observed for the first time at 4 sites in 2009, and then at 7 sites in 2011. In 2014, A. marina was recorded at 2 intertidal sites of the Ria de Aveiro, which were characterized by very fine and medium sand. A. defodiens displayed a wider distribution, being reported at 13 intertidal and 5 subtidal sites where sediment characteristics ranged from mud to medium sand. A. marina densities ranged from 20.5 to 26.0 ind. m(2), higher than those recorded for A. defodiens, which did not exceed 3 ind. m(2). The expansion and the establishment of arenicolids in the Ria de Aveiro lagoon could be a result of the 2009-2010 and 2010-2011 cold winters. Possible consequences of the spread of these bioturbators in this lagunar ecosystem are discussed.
author address [Pires, A.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
reprint address Pires, A (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address adilia@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Science Foundation (FCT) [PEst-C/MAR/LA0017/2013]; Portuguese FCT [PD/00114/2012]; CESAM research funds [BPD/CESAM/RP/BENTONICAS/2013]; National Funds through the Post-doctoral grants [SFRH/BPD/93225/2013, SFRH/BPD/92258/2013]
funding text This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013 and through the Post-doctoral grants to R.M. (SFRH/BPD/93225/2013) and R.F. (SFRH/BPD/92258/2013). L.M. benefited from a PhD grant (PD/00114/2012) given by the Portuguese FCT. A.P. also benefitted from a Post-doctoral grant (BPD/CESAM/RP/BENTONICAS/2013), funded by CESAM research funds. The authors thank Aldiro Pereira and Rui Marques for support during sampling campaigns, Anthony Peter Moreira for the English editing, and Stefania Chiesa for help with genetic analysis.
cited references Allen EJ, 1904, Q J MICROSC SCI, V48, P79; Benedict JE, 1887, ANNELIDA CHAETOPODA, P707; Berke SK, 2010, GLOBAL ECOL BIOGEOGR, V19, P223, DOI 10.1111/j.1466-8238.2009.00509.x; BEUKEMA JJ, 1979, NETH J SEA RES, V13, P331, DOI 10.1016/0077-7579(79)90010-3; BEUKEMA J J, 1976, Netherlands Journal of Sea Research, V10, P236, DOI 10.1016/0077-7579(76)90017-X; Bleidorn C, 2005, MOL PHYLOGENET EVOL, V34, P673, DOI 10.1016/j.ympev.2004.11.019; Byers C, 1978, HYDROBIOLOGIA, V58, P43; CADMAN PS, 1993, J MAR BIOL ASSOC UK, V73, P213; Cadman PS, 1997, J MAR BIOL ASSOC UK, V77, P389; CADMAN PS, 1990, MAR ECOL PROG SER, V64, P107, DOI 10.3354/meps064107; Campbell AL, 2014, ENVIRON SCI TECHNOL, V48, P9745, DOI 10.1021/es502739m; Campoy, 1982, SER ZOOL, V7; Carlton JT, 1995, PB96166525 NTIS US F; Carpenter B, 2005, AQUATIC MACROINVERTE; Costello MJ, 2001, COLLECTION PATRIMOIN; Cunha T, 2005, FISH RES, V76, P56, DOI 10.1016/j.fishres.2005.05.008; Delefosse M, 2012, MAR ECOL PROG SER, V461, P83, DOI 10.3354/meps09821; DEVLAS J, 1979, NETH J SEA RES, V13, P362, DOI 10.1016/0077-7579(79)90012-7; Dias JM, 2000, PHYS CHEM EARTH PT B, V25, P369, DOI 10.1016/S1464-1909(00)00028-9; DOEGLAS DJ, 1968, SEDIMENTOLOGY, V10, P83, DOI 10.1111/j.1365-3091.1968.tb01101.x; Fauchald K, 1977, SCI SERIES, V28; Fauvel P, 1927, FAUNE FRANCE, V16; Figueira E, 2013, ENVIRON SCI POLLUT R, V20, P5682, DOI 10.1007/s11356-013-1587-z; FLACH EC, 1992, NETH J SEA RES, V30, P81, DOI 10.1016/0077-7579(92)90048-J; FLACH EC, 1994, MAR ECOL PROG SER, V115, P139, DOI 10.3354/meps115139; Folmer O., 1994, Molecular Marine Biology and Biotechnology, V3, P294; Freitas R, 2003, ICES J MAR SCI, V60, P599, DOI 10.1016/S1054-3139(03)00061-4; Hall T. A., 1999, NUCL ACIDS S SER, V4, P95, DOI DOI 10.1111/J.1469-8137.2009.02874.X; Hardy Sarah Mincks, 2011, Marine Biodiversity, V41, P195, DOI 10.1007/s12526-010-0056-x; HUTCHINGS P., 2000, FAUNA AUSTR A, P62; JACOBSEN VOGG H., 1967, OPHELIA, V4, P91; Jernshoj KD, 2013, J CHEM PHYS, V139, DOI 10.1063/1.4813920; Lackschewitz D, 1998, HELGOLANDER MEERESUN, V52, P147, DOI 10.1007/BF02908744; Larsonneur C, 1977, J RECH OCEANOGR, V2, P33; Linnaeus C., 1758, SYSTEMA NATURAE REGN; Luttikhuizen PC, 2010, J SEA RES, V63, P17, DOI 10.1016/j.seares.2009.09.001; Malmgren AJ, 1865, NORDISKA HAFS ANNULA, P355; Martins R, 2012, ZOOTAXA, P1; Martins R, 2013, J SEA RES, V83, P173, DOI 10.1016/j.seares.2013.03.001; MCLUSKY DS, 1983, MAR ECOL PROG SER, V11, P173, DOI 10.3354/meps011173; Montserrat F, 2011, J SEA RES, V65, P170, DOI 10.1016/j.seares.2010.09.003; Muller Y, 2004, FAUNE FLORE LITTORAL; Nobre A., 1937, Mem Mus Zool Univ Coimbra Ser 1, V99, P1; Nobre A, 1903, ANN SCI NATURAES POR, V8, P37; Nogueira JMD, 2001, J MAR BIOL ASSOC UK, V81, P415; Novo M, 2011, MOL PHYLOGENET EVOL, V61, P125, DOI 10.1016/j.ympev.2011.05.018; Palumbi SR, 1991, SIMPLE TOOLS GUIDE P; Pires A, 2012, ESTUAR COAST SHELF S, V110, P85, DOI 10.1016/j.ecss.2012.03.027; Pires A, 2012, MAR ECOL-EVOL PERSP, V33, P56, DOI 10.1111/j.1439-0485.2011.00463.x; Pleijel F, 2001, POLYCHAETES; QUINTINO V, 1989, Scientia Marina, V53, P645; Ravara Ascensao, 2013, Check List, V9, P533; REISE K, 1981, MAR ECOL PROG SER, V6, P329, DOI 10.3354/meps006329; REISE K, 1983, MAR BIOL, V74, P327, DOI 10.1007/BF00403459; Rioja, 1931, MEMORIAS ACAD CIENCI; Rodrigues AM, 2011, ESTUAR COAST SHELF S, V95, P338, DOI 10.1016/j.ecss.2011.05.019; Rodrigues AM, 2009, ESTUAR COAST SHELF S, V85, P609, DOI 10.1016/j.ecss.2009.10.004; Rouse GW, 2004, SCIENCE, V305, P668, DOI 10.1126/science.1098650; Rousselot Morgane, 2006, Biotechnology Journal, V1, P333, DOI 10.1002/biot.200500049; Rousset V, 2007, CLADISTICS, V23, P41, DOI 10.1111/j.1096-0031.2006.00128.x; Tosuji Hiroaki, 2012, Plankton & Benthos Research, V7, P195; Schulze A, 2006, BIOL BULL-US, V210, P25, DOI 10.2307/4134534; Stimpson W, 1856, P BOSTON SOC NAT HIS, V5, P110; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI 10.1093/molbev/mst197; Thuillier R, 2011, AM J TRANSPLANT, V11, P1845, DOI 10.1111/j.1600-6143.2011.03614.x; Tosuji Hiroaki, 2010, Plankton & Benthos Research, V5, P242; Valdemarsen T, 2011, J EXP MAR BIOL ECOL, V410, P45, DOI 10.1016/j.jembe.2011.10.006; Vaz N, 2005, OCEAN DYNAM, V55, P416, DOI 10.1007/s10236-005-0015-4; Volkenborn N, 2006, J EXP MAR BIOL ECOL, V330, P169, DOI 10.1016/j.jembe.2005.12.025; Wasson K, 2001, BIOL CONSERV, V102, P143, DOI 10.1016/S0006-3207(01)00098-2; Watson GJ, 1998, MAR ECOL PROG SER, V175, P167, DOI 10.3354/meps175167; Weber M, 1997, AGUDA ENTRE MARES FA; WELLS GP, 1945, J MAR BIOL ASSOC UK, V26, P170; Wells G. P., 1959, Proceedings of the Zoological Society of London, V133, P301; WELLS G. P., 1963, PROC ZOOL SOC LONDON, V140, P121; Wethey DS, 2011, J EXP MAR BIOL ECOL, V400, P132, DOI 10.1016/j.jembe.2011.02.008; Woodin SA, 1998, J MAR RES, V56, P535, DOI 10.1357/002224098321822410
cited reference count 77
publisher INTER-RESEARCH
publisher city OLDENDORF LUHE
publisher address NORDBUNTE 23, D-21385 OLDENDORF LUHE, GERMANY
issn 0171-8630
29-character source abbreviation MAR ECOL PROG SER
iso source abbreviation Mar. Ecol.-Prog. Ser.
publication date AUG 6
year published 2015
volume 533
beginning page 177
ending page 190
digital object identifier (doi) 10.3354/meps11315
page count 14
web of science category Ecology; Marine & Freshwater Biology; Oceanography
subject category Environmental Sciences & Ecology; Marine & Freshwater Biology; Oceanography
document delivery number CO7ZE
unique article identifier WOS:000359382300013
link http://dx.doi.org/10.3354/meps11315