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title Culturable endophytic bacteria from the salt marsh plant Halimione portulacoides: phylogenetic diversity, functional characterization, and influence of metal(loid) contamination
authors Fidalgo, C; Henriques, I; Rocha, J; Tacao, M; Alves, A
author full name Fidalgo, Catia; Henriques, Isabel; Rocha, Jaqueline; Tacao, Marta; Alves, Artur
title Culturable endophytic bacteria from the salt marsh plant Halimione portulacoides: phylogenetic diversity, functional characterization, and influence of metal(loid) contamination
nationality internacional
source ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
language English
document type Article
author keywords Endophyte; Bacteria; Halimione portulacoides; Salt marsh plants; Plant growth promotion; Extracellular enzymes
keywords plus SP NOV.; EMENDED DESCRIPTION; PETROLEUM-HYDROCARBONS; DEGRADING BACTERIA; GROWTH; MERCURY; MICROBACTERIUM; SEDIMENT; PORTUGAL; ROOTS
abstract Halimione portulacoides is abundant in salt marshes, accumulates mercury (Hg), and was proposed as useful for phytoremediation and pollution biomonitoring. Endophytic bacteria promote plant growth and provide compounds with industrial applications. Nevertheless, information about endophytic bacteria from H. portulacoides is scarce. Endophytic isolates (n = 665) were obtained from aboveground and belowground plant tissues, from two Hg-contaminated sites (sites E and B) and a noncontaminated site (site C), in the estuary Ria de Aveiro. Representative isolates (n = 467) were identified by 16S rRNA gene sequencing and subjected to functional assays. Isolates affiliated with Proteobacteria (64 %), Actinobacteria (23 %), Firmicutes (10 %), and Bacteroidetes (3 %). Altererythrobacter (7.4 %), Marinilactibacillus (6.4 %), Microbacterium (10.2 %), Salinicola (8.8 %), and Vibrio (7.8 %) were the most abundant genera. Notably, Salinicola (n = 58) were only isolated from site C; Hoeflea (17), Labrenzia (22), and Microbacterium (67) only from belowground tissues. This is the first report of Marinilactibacillus in the endosphere. Principal coordinate analysis showed that community composition changes with the contamination gradient and tissue. Our results suggest that the endosphere of H. portulacoides represents a diverse bacterial hotspot including putative novel species. Many isolates, particularly those affiliated to Altererythrobacter, Marinilactibacillus, Microbacterium, and Vibrio, tested positive for enzymatic activities and plant growth promoters, exposing H. portulacoides as a source of bacteria and compounds with biotechnological applications.
author address [Fidalgo, Catia; Rocha, Jaqueline; Tacao, Marta; Alves, Artur] Univ Aveiro, Dept Biol, CESAM, P-3800 Aveiro, Portugal; [Fidalgo, Catia; Henriques, Isabel] Univ Aveiro, Dept Biol, iBiMED, Campus Santiago, P-3810193 Aveiro, Portugal; [Fidalgo, Catia; Henriques, Isabel] Univ Aveiro, Dept Biol, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal
reprint address Henriques, I (reprint author), Univ Aveiro, Dept Biol, iBiMED, Campus Santiago, P-3810193 Aveiro, Portugal.; Henriques, I (reprint author), Univ Aveiro, Dept Biol, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal.
e-mail address ihenriques@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh [PTDC/AAC-AMB/118873/2010-FCOMP-01-0124-FEDER-019328]; FCT [UID/AMB/50017/2013]; Institute for Research in Biomedicine [iBiMED-UID/BIM/04501/2013]; [IF/00835/2013]; [IF/00492/2013]; [SFRH/BD/85423/2012]
funding text This work was financed by the European Funds through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh (PTDC/AAC-AMB/118873/2010-FCOMP-01-0124-FEDER-019328). The authors acknowledge FCT financing to CESAM (UID/AMB/50017/2013) and Institute for Research in Biomedicine (iBiMED-UID/BIM/04501/2013), Artur Alves (FCT Investigator Programme-IF/00835/2013), Isabel Henriques (FCT Investigator Programme-IF/00492/2013), and Catia Fidalgo (PhD grant-SFRH/BD/85423/2012).
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cited reference count 72
publisher SPRINGER HEIDELBERG
publisher city HEIDELBERG
publisher address TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
issn 0944-1344
29-character source abbreviation ENVIRON SCI POLLUT R
iso source abbreviation Environ. Sci. Pollut. Res.
publication date MAY
year published 2016
volume 23
issue 10
beginning page 10200
ending page 10214
digital object identifier (doi) 10.1007/s11356-016-6208-1
page count 15
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number DM5WN
unique article identifier WOS:000376421400090
link http://dx.doi.org/10.1007/s11356-016-6208-1