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title Diversity of endophytic Pseudomonas in Halimione portulacoides from metal(loid)-polluted salt marshes
authors Rocha, J; Tacao, M; Fidalgo, C; Alves, A; Henriques, I
author full name Rocha, Jaqueline; Tacao, Marta; Fidalgo, Catia; Alves, Artur; Henriques, Isabel
title Diversity of endophytic Pseudomonas in Halimione portulacoides from metal(loid)-polluted salt marshes
nationality internacional
source ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
language English
document type Article
author keywords Pseudomonas; Halimione portulacoides; Endophytic; Phytoremediation; Plant growth promoters; Metals
keywords plus GROWTH-PROMOTING RHIZOBACTERIA; METAL RESISTANCE; BACTERIAL ENDOPHYTES; PATHOGENIC BACTERIA; ACC DEAMINASE; PLANT; ACCUMULATION; STRAINS; SITE; L.
abstract Phytoremediation assisted by bacteria is seen as a promising alternative to reduce metal contamination in the environment. The main goal of this study was to characterize endophytic Pseudomonas isolated from Halimione portulacoides, a metal-accumulator plant, in salt marshes contaminated with metal(loid)s. Phylogenetic analysis based on 16S rRNA and gyrB genes showed that isolates affiliated with P. sabulinigri (n = 16), P. koreensis (n = 10), P. simiae (n = 5), P. seleniipraecipitans (n = 2), P. guineae (n = 2), P. migulae (n = 1), P. fragi (n = 1), P. xanthomarina (n = 1), and Pseudomonas sp. (n = 1). Most of these species have never been described as endophytic. The majority of the isolates were resistant to three or more metal(loid)s. Antibiotic resistance was frequent among the isolates but most likely related to species-intrinsic features. Common acquired antibiotic resistance genes and integrons were not detected. Plasmids were detected in 43.6 % of the isolates. Isolates that affiliated with different species shared the same plasmid profile but attempts to transfer metal resistance to receptor strains were not successful. Phosphate solubilization and IAA production were the most prevalent plant growth promoting traits, and 20 % of the isolates showed activity against phytopathogenic bacteria. Most isolates produced four or more extracellular enzymes. Preliminary results showed that two selected isolates promote Arabidopsis thaliana root elongation. Results highlight the diversity of endophytic Pseudomonas in H. portulacoides from contaminated sites and their potential to assist phytoremediation by acting as plant growth promoters and as environmental detoxifiers.
author address [Rocha, Jaqueline; Alves, Artur] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Rocha, Jaqueline; Alves, Artur] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Tacao, Marta; Fidalgo, Catia; Henriques, Isabel] Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro, Portugal; [Tacao, Marta; Fidalgo, Catia; Henriques, Isabel] Univ Aveiro, iBiMED, P-3810193 Aveiro, Portugal
reprint address Alves, A (reprint author), Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal.; Alves, A (reprint author), Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal.
e-mail address artur.alves@ua.pt
researcherid number Alves, Artur/C-3645-2008; Tacao, Marta/C-9785-2009
orcid number Alves, Artur/0000-0003-0117-2958; Tacao, Marta/0000-0001-7965-4928
funding agency and grant number European Funds (FEDER) through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AAC-651 AMB/118873/2010-FCOMP-01-0124-FEDER-019328]; FCT [UID/AMB/50017/2013, UID/BIM/04501/2013, IF/00835/2013, IF/00492/2013, SFRH/BD/85423/2012]
funding text This work was supported by European Funds (FEDER) through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh (PTDC/AAC-651 AMB/118873/2010-FCOMP-01-0124-FEDER-019328). Authors also acknowledge FCT financing to CESAM (UID/AMB/50017/2013) and iBiMED (UID/BIM/04501/2013), Artur Alves (FCT Investigator Programme-IF/00835/2013), Isabel Henriques (FCT Investigator Programme-IF/00492/2013) and Catia Fidalgo (PhD grant SFRH/BD/85423/2012). The authors wish to thank Sofia Pereira and Paula Castro (Universidade Catolica Portuguesa, Portugal) for providing positive controls for plant-growth promotion traits screening, Kornelia Smalla (Julius Kuhn Institut, Germany) for the phytopathogenic strains used in antimicrobial activity assays.
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publisher SPRINGER HEIDELBERG
publisher city HEIDELBERG
publisher address TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
issn 0944-1344
29-character source abbreviation ENVIRON SCI POLLUT R
iso source abbreviation Environ. Sci. Pollut. Res.
publication date JUL
year published 2016
volume 23
issue 13
beginning page 13255
ending page 13267
digital object identifier (doi) 10.1007/s11356-016-6483-x
page count 13
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number DP9LI
unique article identifier WOS:000378817300061
link doi:10.1007/s11356-016-6483-x.