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title Depicting how Eucalyptus globulus survives drought: involvement of redox and DNA methylation events
authors Correia, B; Valledor, L; Hancock, RD; Jesus, C; Amaral, J; Meijon, M; Pinto, G
author full name Correia, Barbara; Valledor, Luis; Hancock, Robert D.; Jesus, Claudia; Amaral, Joana; Meijon, Monica; Pinto, Gloria
title Depicting how Eucalyptus globulus survives drought: involvement of redox and DNA methylation events
nationality internacional
source FUNCTIONAL PLANT BIOLOGY
language English
document type Article
author keywords antioxidant; ascorbate-glutathione cycle; epigenetics; oxidative stress; recovery; water stress
keywords plus ASCORBATE-GLUTATHIONE CYCLE; LEAF WATER RELATIONS; HISTONE ACETYLATION; ALPHA-TOCOPHEROL; GENE-EXPRESSION; CLIMATE-CHANGE; STRESS; ARABIDOPSIS; RESPONSES; DEFICIT
abstract Eucalyptus globulus Labill. is widely cultivated and used by industry but its productivity is currently restricted by drought events, so research focussing on supporting programs to breed adapted germplasm is needed. In the present work we monitored severity of acute drought stress (7 and 11 days after water withholding) and relief (2h and 3 days after rewatering) by quantifying several biochemical markers of oxidative stress and DNA methylation patterns in leaves. Water withholding imposed a mild oxidative stress as estimated by redox shifts in the major antioxidant pools and increased lipid peroxidation. At the DNA level, global 5-methylcytosine distribution increased over the dehydration period especially in vascular tissue as estimated by immunolocalisation. Using methylation-sensitive RAPD analysis, which discriminates methylation changes occurring in specific DNA sequences, we found a high number of specific demethylation events also taking place. Immunolocalisation indicated a rapid reduction in global DNA methylation 2h after rehydration; however, a large number of de novo methylation events were still detected by methylation-sensitive RAPD. These events were associated with decreased lipid peroxidation and high cellular GSH pools relative to unstressed plants. Our results indicate the parallel induction of redox and complex DNA methylation changes occurring during stress imposition and relief.
author address [Correia, Barbara; Jesus, Claudia; Amaral, Joana; Pinto, Gloria] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Correia, Barbara; Jesus, Claudia; Amaral, Joana; Pinto, Gloria] Univ Aveiro, Ctr Environm & Marine Studies CESAM, P-3810193 Aveiro, Portugal; [Valledor, Luis; Meijon, Monica] Univ Oviedo, Plant Physiol, Dept Organisms & Syst Biol, Oviedo 33005, Spain; [Hancock, Robert D.] James Hutton Inst, Cell & Mol Sci, Dundee DD2 5DA, Scotland
reprint address Pinto, G (reprint author), Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal.; Pinto, G (reprint author), Univ Aveiro, Ctr Environm & Marine Studies CESAM, P-3810193 Aveiro, Portugal.
e-mail address gpinto@ua.pt
funding agency and grant number FEDER through COMPETE (Programa Operacional Fatores de Competitividade); National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-FOR/3807/2012 - FCOMP-01-0124-FEDER-027979, UID/AMB/50017/2013, PTDC/AGR-CFL/112996/2009]; FCT/MEC; FEDER [UID/AMB/50017]; FCT [SFRH/BD/86448/2012, SFRH/BDP/89765/2012, SFRH/BPD/101669/2014]; Ramon y Cajal Program (Spanish Ministry of Economy and Competitiveness) [RYC-2014-14981]; Rural and Environment Science and Analytical Services Division of the Scottish Government
funding text This research was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade) and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within the projects PANDORA (PTDC/AGR-FOR/3807/2012 - FCOMP-01-0124-FEDER-027979), UID/AMB/50017/2013 and PTDC/AGR-CFL/112996/2009. FCT/MEC, through national funds, and co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020 provide financial support to CESAM (UID/AMB/50017). FCT also supported the fellowships of B Correia (SFRH/BD/86448/2012), L Valledor (SFRH/BDP/89765/2012) and G Pinto (SFRH/BPD/101669/2014). M Meijon was supported by the Ramon y Cajal Program (RYC-2014-14981) (Spanish Ministry of Economy and Competitiveness). The James Hutton Institute receives support from by the Rural and Environment Science and Analytical Services Division of the Scottish Government. We appreciate Professor Amparo Faustino from University of Aveiro (Department of Chemistry) for her valuable assistance in H2O2 measurements.
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cited reference count 66
publisher CSIRO PUBLISHING
publisher city CLAYTON
publisher address UNIPARK, BLDG 1, LEVEL 1, 195 WELLINGTON RD, LOCKED BAG 10, CLAYTON, VIC 3168, AUSTRALIA
issn 1445-4408
29-character source abbreviation FUNCT PLANT BIOL
iso source abbreviation Funct. Plant Biol.
year published 2016
volume 43
issue 9
beginning page 838
ending page 850
digital object identifier (doi) 10.1071/FP16064
page count 13
web of science category Plant Sciences
subject category Plant Sciences
document delivery number DV0YK
unique article identifier WOS:000382646500004