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title Co-selection of antibiotic and metal(loid) resistance in gram-negative epiphytic bacteria from contaminated salt marshes
authors Henriques, I; Tacao, M; Leite, L; Fidalgo, C; Araujo, S; Oliveira, C; Alves, A
author full name Henriques, Isabel; Tacao, Marta; Leite, Laura; Fidalgo, Catia; Araujo, Susana; Oliveira, Claudia; Alves, Artur
title Co-selection of antibiotic and metal(loid) resistance in gram-negative epiphytic bacteria from contaminated salt marshes
nationality internacional
source MARINE POLLUTION BULLETIN
language English
document type Article
author keywords Antibiotic resistance genes; Mobile genetic elements; Halimione portulacoides; merA gene; Plant surface
keywords plus PSEUDOMONAS-AERUGINOSA; MERCURY RESISTANCE; METAL RESISTANCE; AGRICULTURAL SOILS; HEAVY-METALS; ENVIRONMENT; DIVERSITY; MULTIDRUG; INTEGRONS; HEALTH
abstract The goal of this study was to investigate co-selection of antibiotic resistance in gram-negative epiphytic bacteria. Halimione portulacoides samples were collected from metal(loid)-contaminated and non-contaminated salt marshes. Bacterial isolates (n = 137) affiliated with Vibrio, Pseudomonas, Shewanella, Comamonas, Aeromonas and with Enterobacteriaceae. Vibrio isolates were more frequent in control site while Pseudomonas was common in contaminated sites. Metal(loid) and antibiotic resistance phenotypes varied significantly according to site contamination, and multiresistance was more frequent in contaminated sites. However, differences among sites were not observed in terms of prevalence or diversity of acquired antibiotic resistance genes, integrons and plasmids. Gene merA, encoding mercury resistance, was only detected in isolates from contaminated sites, most of which were multiresistant to antibiotics. Results indicate that metal(loid) contamination selects for antibiotic resistance in plant surfaces. In salt marshes, antibiotic resistance may be subsequently transferred to other environmental compartments, such as estuarine water or animals, with potential human health risks. (C) 2016 Elsevier Ltd. All rights reserved.
author address [Henriques, Isabel; Tacao, Marta; Leite, Laura; Fidalgo, Catia; Araujo, Susana; Oliveira, Claudia; Alves, Artur] Univ Aveiro, Dept Biol, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal; [Henriques, Isabel; Tacao, Marta; Fidalgo, Catia; Araujo, Susana] Univ Aveiro, IBiMED, Campus Santiago, P-3810193 Aveiro, Portugal
reprint address Henriques, I (reprint author), Univ Aveiro, Dept Biol, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal.; Henriques, I (reprint author), Univ Aveiro, IBiMED, Campus Santiago, P-3810193 Aveiro, Portugal.
e-mail address ihenriques@ua.pt
funding agency and grant number European Funds (FEDER) through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AAC - 651 AMB/118873/2010 - FCOMP-01-0124-FEDER-019328]; FCT [UID/AMB/50017/2013, UID/BIM/04501/2013, IF/00492/2013, SFRH/BD/85423/2012, SFRH/BPD/65820/2009]
funding text Authors gratefully acknowledge Dr. A. Carattoli for the positive controls for detection of lasmid replicons. This work was supported by European Funds (FEDER) through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) (project PhytoMarsh PTDC/AAC - 651 AMB/118873/2010 - FCOMP-01-0124-FEDER-019328). Authors also acknowledge FCT financing to CESAM (UID/AMB/50017/2013) and iBiMED (UID/BIM/04501/2013), Artur Alves (FCT Investigator Programme IF/00492/2013), Isabel Henriques (FCT Investigator Programme IF/00492/2013), Catia Fidalgo (PhD grant SFRH/BD/85423/2012) and Claudia Oliveira (SFRH/BPD/65820/2009).
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cited reference count 53
publisher PERGAMON-ELSEVIER SCIENCE LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
issn 0025-326X
29-character source abbreviation MAR POLLUT BULL
iso source abbreviation Mar. Pollut. Bull.
publication date AUG 15
year published 2016
volume 109
issue 1
beginning page 427
ending page 434
digital object identifier (doi) 10.1016/j.marpolbul.2016.05.031
page count 8
web of science category Environmental Sciences; Marine & Freshwater Biology
subject category Environmental Sciences & Ecology; Marine & Freshwater Biology
document delivery number DT7EP
unique article identifier WOS:000381650200061
link http://dx.doi.org/10.1016/j.marpolbul.2016.05.031