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title Microbacterium diaminobutyricum sp nov., isolated from Halimione portulacoides, which contains diaminobutyric acid in its cell wall, and emended description of the genus Microbacterium
authors Fidalgo, C; Riesco, R; Henriques, I; Trujillo, ME; Alves, A
author full name Fidalgo, Catia; Riesco, Raul; Henriques, Isabel; Trujillo, Martha E.; Alves, Artur
title Microbacterium diaminobutyricum sp nov., isolated from Halimione portulacoides, which contains diaminobutyric acid in its cell wall, and emended description of the genus Microbacterium
nationality internacional
source INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
language English
document type Article
keywords plus GENERA MICROBACTERIUM; DEOXYRIBONUCLEIC-ACID; LIPID-COMPOSITION; ROOT-NODULES; IDENTIFICATION; CHROMATOGRAPHY; HYBRIDIZATION; ENDOPHYTES; AL.; DNA
abstract Three actinobacterial strains were isolated from roots of the salt-marsh plant Halimione portulacoides collected in Ria de Aveiro, Portugal. Molecular typing using enterobacterial repetitive intergenic consensus ERIC-PCR fingerprinting showed the strains to be highly similar. Phylogenetic analyses based on the 16S rRNA gene sequence and multilocus sequence analysis (MLSA) using gyrB, rpoB, recA and ppk and 16S rRNA genes sequences showed that the strains represented a member of the genus Microbacterium, with Microbacterium lacus DSM 18910(T) as the closest phylogenetic relative. DNA-DNA hybridization between strain RZ63(T) and its closest relative was below 70 %, supporting the hypothesis that it represented a distinct genomic species. Chemotaxonomic analyses of the novel strains and their DNA G+C contents confirmed their affiliation to the genus Microbacterium, however, the peptidoglycan of RZ63T contained diaminobutyric acid as the diagnostic diamino acid. In addition, physiological and fatty acid analyses revealed differences between these strains and their phylogenetic relatives, reinforcing their status as a distinct species. Based on the physiological, genetic and chemotaxonomic characterisation it is proposed that the strains studied represent a novel species of the genus Microbacterium for which the name Microbacterium diaminobutyricum sp. nov. is proposed (type strain RZ63(T) = DSM 27101(T) = CECT 8355(T)).
author address [Fidalgo, Catia; Henriques, Isabel; Alves, Artur] Univ Aveiro, Dep Biol, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal; [Fidalgo, Catia; Henriques, Isabel] Univ Aveiro, Dep Biol, IBiMED, Campus Santiago, P-3810193 Aveiro, Portugal; [Riesco, Raul; Trujillo, Martha E.] Univ Salamanca, Dept Microbiol & Genet, Lab 214, Campus Miguel Unamuno,Edific Dept, Salamanca 37007, Spain
reprint address Alves, A (reprint author), Univ Aveiro, Dep Biol, CESAM, Campus Santiago, P-3810193 Aveiro, Portugal.
e-mail address artur.alves@ua.pt
funding agency and grant number European Funds (FEDER) through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) within project Phyto-Marsh [PTDC/AAC-AMB/118873/2010, FCOMP-01-0124-FEDER019328]; Ministerio de Economia y Competitividad [CGL2014-52735-P]
funding text This work was supported by European Funds (FEDER) through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within project Phyto-Marsh (PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER019328). FCT is acknowledged for financing to the Centre for Environmental and Marine Studies (CESAM) (UID/AMB/50017/2013), the instiute for research in Biomedicine (iBiMED) (UID/BIM/04501/2013), Artur Alves (FCT Investigator Programme - IF/00835/2013), Isabel Henriques (FCT Investigator Programme - IF/00492/2013) and Catia Fidalgo (PhD grant - SFRH/BD/85423/2012). M. E. Trujillo was funded by the Ministerio de Economia y Competitividad under project CGL2014-52735-P.
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cited reference count 42
publisher MICROBIOLOGY SOC
publisher city LONDON
publisher address CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND
issn 1466-5026
29-character source abbreviation INT J SYST EVOL MICR
iso source abbreviation Int. J. Syst. Evol. Microbiol.
publication date NOV
year published 2016
volume 66
part number 11
beginning page 4492
ending page 4500
digital object identifier (doi) 10.1099/ijsem.0.001379
page count 9
web of science category Microbiology
subject category Microbiology
document delivery number EF9YE
unique article identifier WOS:000390686600028
link http://dx.doi.org/10.1099/ijsem.0.001379