HOME  »  Members  »  Collaborators  »  PhD Students/Fellows
 
title Integrated proteomics and metabolomics to unlock global and clonal responses of Eucalyptus globulus recovery from water deficit
authors Correia, B; Valledor, L; Hancock, RD; Renaut, J; Pascual, J; Soares, AMVM; Pinto, G
author full name Correia, Barbara; Valledor, Luis; Hancock, Robert D.; Renaut, Jenny; Pascual, Jesus; Soares, Amadeu M. V. M.; Pinto, Gloria
title Integrated proteomics and metabolomics to unlock global and clonal responses of Eucalyptus globulus recovery from water deficit
nationality internacional
source METABOLOMICS
language English
document type Article
author keywords DIGE; Forest tree; GC-MS; Plant; Stress
keywords plus CYTOSOLIC GLUTAMINE-SYNTHETASE; ABIOTIC STRESSES; DROUGHT STRESS; PLANT; TOLERANCE; LEAF; METABOLITES; NETWORKS; GROWTH; ADAPTATION
abstract Background and aims Water availability is well known for impacting productivity of Eucalyptus but comprehensive knowledge on cellular pathways involved in recovery and tolerance is scarce. In this context, we aimed to unveil putative mechanisms that account for drought recovery of E. globulus, and to identify specific strategies that make a clone more adapted to water deficit. Methods We resorted to comparative proteome (using difference gel electrophoresis) and metabolome [using Gas chromatography-mass spectrometry (GC-MS)] analyses in two E. globulus clones that exhibit physiological differences in their capacity to tolerate water shortage and restoration; also, interpretable networks were constructed coupled with previously assessed physiological matrices in order to interrogate the large datasets generated and develop a clear and integrative analysis. Results Our study enabled the separation and isolation of 2031 peptide spots, 217 of which were identified. GC-MS yielded the detection of 121 polar metabolites. Water shortage negatively affected photosynthesis, gene regulation, cell growth and secondary metabolites; enhanced photo protection, osmoprotection, and other defence-related pathways; and caused a shift from chloroplastic to mitochondrial energy generation. Recovery was characterised by upregulation of all previously described pathways. The analysis of the resilient clone AL-18, which presented a network very distinct from the responsive clone AL-10, reinforced the role of specific photosynthetic and defence-related proteins as key players in mediating drought tolerance and revealed new players: glutamine synthetase, malate dehydrogenase and isoflavone reductase-like protein. Conclusion This study provides a set of novel proteins and pathways involved in drought stress that represent potential drought tolerance markers for early selection of Eucalyptus.
author address [Correia, Barbara; Soares, Amadeu M. V. M.; Pinto, Gloria] Univ Aveiro, Dept Biol, Aveiro, Portugal; [Correia, Barbara; Soares, Amadeu M. V. M.; Pinto, Gloria] Univ Aveiro, Ctr Environm & Marine Studies CESAM, Aveiro, Portugal; [Valledor, Luis; Pascual, Jesus] Univ Oviedo, Dept Organisms & Syst Biol, Plant Physiol, Oviedo, Spain; [Hancock, Robert D.] James Hutton Inst, Cell & Mol Sci, Dundee, Scotland; [Renaut, Jenny] Luxembourg Inst Sci & Technol, Environm Res & Innovat ERIN Dept, Belvaux, Luxembourg
reprint address Pinto, G (reprint author), Univ Aveiro, Dept Biol, Aveiro, Portugal.; Pinto, G (reprint author), Univ Aveiro, Ctr Environm & Marine Studies CESAM, Aveiro, Portugal.
e-mail address gpinto@ua.pt
funding agency and grant number Fundo Europeu de Desenvolvimento Regional (FEDER) through Programa Operacional Fatores de Competitividade (COMPETE); National Funds through Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-CFL/112996/2009]; FCT/MEC through national funds; FEDER [CESAM - UID/AMB/50017]; FCT [SFRH/BD/86448/2012, SFRH/BPD/101669/2014]; Rural and Environment Science and Analytical Services Division of the Scottish Government
funding text This research was supported by Fundo Europeu de Desenvolvimento Regional (FEDER) through Programa Operacional Fatores de Competitividade (COMPETE), and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within the Project PTDC/AGR-CFL/112996/2009. FCT/MEC, through national funds, and co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020 provide financial support to Centre for Environmental and Marine Studies (CESAM - UID/AMB/50017). FCT also supported the fellowships of Barbara Correia (SFRH/BD/86448/2012) and Gloria Pinto (SFRH/BPD/101669/2014). The James Hutton Institute receives support from by the Rural and Environment Science and Analytical Services Division of the Scottish Government. We thank Altri florestal for providing the plant material, and Lucinda Neves and Marta Pinto-Marijuan for technical support.
cited references Asada K, 2006, PLANT PHYSIOL, V141, P391, DOI 10.1104/pp.106.082040; Bernard SM, 2009, NEW PHYTOL, V182, P608, DOI 10.1111/j.1469-8137.2009.02823.x; Brossa R, 2015, PLANTA, V241, P803, DOI 10.1007/s00425-014-2221-0; Chaves MM, 2003, FUNCT PLANT BIOL, V30, P239, DOI 10.1071/FP02076; Cheynier V, 2013, PLANT PHYSIOL BIOCH, V72, P1, DOI 10.1016/j.plaphy.2013.05.009; Coopman Rafael E, 2008, Electron. J. Biotechnol., V11, P30; Correia B, 2014, PHYSIOL PLANTARUM, V150, P580, DOI 10.1111/ppl.12110; Correia B, 2014, PLANT PHYSIOL BIOCH, V82, P151, DOI 10.1016/j.plaphy.2014.05.016; Costa E., 2004, TREE PHYSIOL, V24, P1165; Valdes AE, 2013, J PROTEOMICS, V79, P263, DOI 10.1016/j.jprot.2012.12.019; Feller U., 2008, General and Applied Plant Physiology, V34, P5; Foyer CH, 2016, J EXP BOT, V67, P2025, DOI 10.1093/jxb/erw079; Freeman JS, 2013, NEW PHYTOL, V198, P1121, DOI 10.1111/nph.12237; Furuhashi T, 2012, J PLANT INTERACT, V7, P84, DOI 10.1080/17429145.2011.603059; Galmes J, 2007, NEW PHYTOL, V175, P81, DOI 10.1111/j.1469-8137.2007.02087.x; Gonzalez I., 2011, MIXOOMICS OMICS DATA; Granda V, 2011, J PLANT PHYSIOL, V168, P661, DOI 10.1016/j.jplph.2010.09.015; Kaminski KP, 2015, THEOR APPL GENET, V128, P2143, DOI 10.1007/s00122-015-2573-2; Katam R, 2016, J PROTEOMICS, V143, P209, DOI 10.1016/j.jprot.2016.05.031; Kottapalli KR, 2009, PLANT CELL ENVIRON, V32, P380, DOI 10.1111/j.1365-3040.2009.01933.x; Krasensky J, 2012, J EXP BOT, V63, P1593, DOI 10.1093/jxb/err460; Liu DW, 2013, PROTEOMICS, V13, P2046, DOI 10.1002/pmic.201200425; McKiernan AB, 2014, ENVIRON EXP BOT, V105, P55, DOI 10.1016/j.envexpbot.2014.04.008; McKiernan AB, 2016, TREE PHYSIOL, V36, P133, DOI 10.1093/treephys/tpv106; Pastore D, 2007, J EXP BOT, V58, P195, DOI 10.1093/jxb/erl273; Printz B, 2013, J PROTEOME RES, V12, P5160, DOI 10.1021/pr400590d; Salekdeh GH, 2002, PROTEOMICS, V2, P1131, DOI 10.1002/1615-9861(200209)2:9<1131::AID-PROT1131>3.0.CO;2-1; Sanchez DH, 2008, PHYSIOL PLANTARUM, V132, P209, DOI 10.1111/j.1399-3054.2007.00993.x; Scalabrin E, 2015, ANAL BIOANAL CHEM, V407, P6357, DOI 10.1007/s00216-015-8770-7; Sergeant K, 2011, J PROTEOMICS, V74, P1385, DOI 10.1016/j.jprot.2011.03.011; Shvaleva AL, 2006, TREE PHYSIOL, V26, P239; Tomaz T, 2010, PLANT PHYSIOL, V154, P1143, DOI 10.1104/pp.110.161612; Valledor L, 2013, MOL CELL PROTEOMICS, V12, P2032, DOI 10.1074/mcp.M112.026765; Valledor L, 2011, J PROTEOMICS, V74, P1, DOI 10.1016/j.jprot.2010.07.007; Vitamvas P, 2012, PROTEOMICS, V12, P68, DOI 10.1002/pmic.201000779; Warren CR, 2012, METABOLOMICS, V8, P186, DOI 10.1007/s11306-011-0299-y; Warren CR, 2011, PLANT CELL ENVIRON, V34, P1609, DOI 10.1111/j.1365-3040.2011.02357.x; Weckwerth W, 2004, PROTEOMICS, V4, P78, DOI 10.1002/pmic.200200500; WOLOSIUK RA, 1978, ARCH BIOCHEM BIOPHYS, V189, P97, DOI 10.1016/0003-9861(78)90119-4; Zhang N, 1999, P NATL ACAD SCI USA, V96, P9438, DOI 10.1073/pnas.96.16.9438
cited reference count 40
publisher SPRINGER
publisher city NEW YORK
publisher address 233 SPRING ST, NEW YORK, NY 10013 USA
issn 1573-3882
29-character source abbreviation METABOLOMICS
iso source abbreviation Metabolomics
publication date AUG
year published 2016
volume 12
issue 8
article number 141
digital object identifier (doi) 10.1007/s11306-016-1088-4
page count 12
web of science category Endocrinology & Metabolism
subject category Endocrinology & Metabolism
document delivery number DS9DO
unique article identifier WOS:000381083100015