HOME  »  Members  »  Collaborators  »  PhD Students/Fellows
 
title Mating type genes in the genus Neofusicoccum: Mating strategies and usefulness in species delimitation
authors Lopes, A; Phillips, AJL; Alves, A
author full name Lopes, Anabela; Phillips, Alan J. L.; Alves, Artur
title Mating type genes in the genus Neofusicoccum: Mating strategies and usefulness in species delimitation
nationality internacional
source FUNGAL BIOLOGY
language English
document type Article
author keywords Botryosphaeriaceae; MAT genes; PCR-based assay; Phylogeny; Reproductive strategies
keywords plus MOLECULAR-ORGANIZATION; RIBIS COMPLEX; SP-NOV; BOTRYOSPHAERIACEAE; EVOLUTION; PATHOGEN; LOCI; SEQUENCES; RECOGNITION; DIVERSITY
abstract The genus Neofusicoccum includes species with wide geographical and plant host distribution, some of them of economic importance. The genus currently comprises 27 species that are difficult to identify based on morphological features alone. Thus, species differentiation is based on phylogenetic species recognition using multigene genealogies. In this study, we characterised the mating type genes of Neofusicoccum species. Specific primers were designed to amplify and sequence MAT genes in several species and a PCR-based mating type diagnostic assay was developed. Homothallism was the predominant mating strategy among the species tested. Furthermore, the potential of mating type gene sequences for species delimitation was evaluated. Phylogenetic analyses were performed on both MAT genes and compared with multigene genealogies using sequences of the ribosomal internal transcribed spacer region, translation elongation factor 1-alpha and beta-tubulin. Phylogenies based on mating type genes could discriminate between the species analysed and are in concordance with the results obtained with the more conventional multilocus phylogenetic analysis approach. Thus, MAT genes represent a powerful tool to delimit cryptic species in the genus Neofusicoccum. (C) 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
author address [Lopes, Anabela; Alves, Artur] Univ Aveiro, CESAM, Biol Dept, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Phillips, Alan J. L.] Univ Lisbon, Fac Sci Biosyst & Integrat Sci Inst BioISI, P-1749016 Lisbon, Portugal
reprint address Alves, A (reprint author), Univ Aveiro, CESAM, Biol Dept, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address artur.alves@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788]; CESAM [UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638]; FCT Investigator Programme [IF/00835/2013]; PhD grant [SFRH/BD/85615/2012]
funding text This work was financed by European Funds through COMPETE and National Funds through the Portuguese Foundation for Science and Technology (FCT) to project ALIEN (PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788), CESAM (UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638), Artur Alves (FCT Investigator Programme - IF/00835/2013) anAnabela Lopes (PhD grant - SFRH/BD/85615/2012). We are thankful to Benedetto Linaldeddu (University of Sassari, Italy), Vincenzo Mondello (University of Palermo, Italy) and Akila Berraf-Tebbal (University of Blida, Algeria) for sharing some of their isolates.
cited references Alves A, 2008, FUNGAL DIVERS, V28, P1; Alves A, 2004, MYCOLOGIA, V96, P598, DOI 10.2307/3762177; Bennett RS, 2003, FUNGAL GENET BIOL, V40, P25, DOI 10.1016/S1087-1845(03)00062-8; Berraf-Tebbal A, 2014, PHYTOPATHOL MEDITERR, V53, P416; Bihon W, 2014, FUNGAL GENET BIOL, V62, P55, DOI 10.1016/j.fgb.2013.10.013; Blanco-Ulate B, 2013, GENOME ANNOUNCEMENTS, V1, P1, DOI [10.1128/genomeA.00228-13, DOI 10.1128/GENOMEA.00339-13, 10.1128/genomeA.00339-13]; Coppin E, 1997, MICROBIOL MOL BIOL R, V61, P411; Crous PW, 2006, STUD MYCOL, P235; Debuchy R, 2006, MYCOTA, P293, DOI DOI 10.1007/3-540-28135-5_15; Du MZ, 2005, MYCOLOGIA, V97, P641, DOI 10.3852/mycologia.97.3.641; Fraser JA, 2007, EUKARYOT CELL, V6, P622, DOI 10.1128/EC.00018-07; Galagan JE, 2005, NATURE, V438, P1105, DOI 10.1038/nature04341; Gioti A, 2012, MOL BIOL EVOL, V29, P3215, DOI 10.1093/molbev/mss132; GLASS NL, 1995, APPL ENVIRON MICROB, V61, P1323; Groenewald M, 2007, PHYTOPATHOLOGY, V97, P825, DOI 10.1094/PHYTO-97-7-0825; Groenewald M, 2006, FUNGAL GENET BIOL, V43, P813, DOI 10.1016/j.fgb.2006.05.008; Hall TA., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Inderbitzin P, 2005, P NATL ACAD SCI USA, V102, P11390, DOI 10.1073/pnas.0501918102; Kashyap PL, 2014, J BASIC MICROB, V54, P1; Kronstad JW, 1997, ANNU REV GENET, V31, P245, DOI 10.1146/annurev.genet.31.1.245; Lee SC, 2010, MICROBIOL MOL BIOL R, V74, P298, DOI 10.1128/MMBR.00005-10; Martin T, 2010, PLOS ONE, V5, DOI 10.1371/journal.pone.0015199; McGuire IC, 2001, FUNGAL GENET BIOL, V34, P131, DOI 10.1006/fgbi.2001.1295; O'Donnell K, 2004, FUNGAL GENET BIOL, V41, P600, DOI 10.1016/j.fgb.2004.03.003; ODonnell K, 1997, MOL PHYLOGENET EVOL, V7, P103, DOI 10.1006/mpev.1996.0376; Pavlic D, 2009, MYCOLOGIA, V101, P636, DOI 10.3852/08-193; Pavlic D, 2009, MOL PHYLOGENET EVOL, V51, P259, DOI 10.1016/j.ympev.2008.12.017; Phillips A, 2005, MYCOLOGIA, V97, P513, DOI 10.3852/mycologia.97.2.513; Phillips AJL, 2013, STUD MYCOL, P51, DOI 10.3114/sim0021; Poggeler S, 2011, FUNGAL BIOL-UK, V115, P615, DOI 10.1016/j.funbio.2011.03.003; Poggeler S, 1999, CURR GENET, V36, P222, DOI 10.1007/s002940050494; Putman AI, 2015, FUNGAL GENET BIOL, V81, P25, DOI 10.1016/j.fgb.2015.05.012; Rydholm C, 2007, EUKARYOT CELL, V6, P868, DOI 10.1128/EC.00319-06; Sakalidis ML, 2011, MOL PHYLOGENET EVOL, V60, P333, DOI 10.1016/j.ympev.2011.04.026; Slippers B, 2004, MYCOLOGIA, V96, P1030, DOI 10.2307/3762087; Slippers Bernard, 2007, Fungal Biology Reviews, V21, P90, DOI 10.1016/j.fbr.2007.06.002; Steenkamp ET, 2000, APPL ENVIRON MICROB, V66, P4378, DOI 10.1128/AEM.66.10.4378-4382.2000; Stergiopoulos I, 2007, FUNGAL GENET BIOL, V44, P415, DOI 10.1016/j.fgb.2006.11.004; Strandberg R, 2010, FUNGAL GENET BIOL, V47, P869, DOI 10.1016/j.fgb.2010.06.008; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI 10.1093/molbev/mst197; Taylor JW, 2000, FUNGAL GENET BIOL, V31, P21, DOI 10.1006/fgbi.2000.1228; Thompson JD, 1997, NUCLEIC ACIDS RES, V25, P4876, DOI 10.1093/nar/25.24.4876; Turgeon BG, 1998, ANNU REV PHYTOPATHOL, V36, P115, DOI 10.1146/annurev.phyto.36.1.115; Turgeon G, 2000, FUNGAL GENET BIOL, V31, P1, DOI 10.1006/fgbi.2000.1227; Ueng PP, 2003, CURR GENET, V43, P121, DOI 10.1007/s00294-003-0377-4; Wey T, 2016, FUNGAL GENET BIOL, V87, P54, DOI 10.1016/j.fgb.2015.12.013; White TJ, 1990, PCR PROTOCOLS GUIDE, V18, P315, DOI DOI 10.1139/B07-071; Wik L, 2008, BMC EVOL BIOL, V8, DOI 10.1186/1471-2148-8-109; Witthuhn RC, 2000, MYCOLOGIA, V92, P447, DOI 10.2307/3761503; Yokoyama E, 2006, FEMS MICROBIOL LETT, V264, P182, DOI 10.1111/j.1574-6968.2006.00447.x; Yun SH, 1999, P NATL ACAD SCI USA, V96, P5592, DOI 10.1073/pnas.96.10.5592; Yun SH, 2000, FUNGAL GENET BIOL, V31, P7, DOI 10.1006/fgbi.2000.1226; Zaffarano PL, 2010, FUNGAL GENET BIOL, V47, P761, DOI 10.1016/j.fgb.2010.06.001; Zhan J, 2002, FUNGAL GENET BIOL, V36, P128, DOI 10.1016/S1087-1845(02)00013-0
cited reference count 54
times cited 1
total times cited count (wos, bci, and cscd) 1
publisher ELSEVIER SCI LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
issn 1878-6146
29-character source abbreviation FUNGAL BIOL-UK
iso source abbreviation Fungal Biol.
publication date APR
year published 2017
volume 121
issue 4
special issue SI
beginning page 394
ending page 404
digital object identifier (doi) 10.1016/j.funbio.2016.08.011
page count 11
web of science category Mycology
subject category Mycology
document delivery number ES9AG
unique article identifier WOS:000399847700006
link http://dx.doi.org/10.1016/j.funbio.2016.08.011