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title Mating type genes in the genus Neofusicoccum: Mating strategies and usefulness in species delimitation
authors Lopes, A; Phillips, AJL; Alves, A
author full name Lopes, Anabela; Phillips, Alan J. L.; Alves, Artur
title Mating type genes in the genus Neofusicoccum: Mating strategies and usefulness in species delimitation
nationality internacional
language English
document type Article
author keywords Botryosphaeriaceae; MAT genes; PCR-based assay; Phylogeny; Reproductive strategies
abstract The genus Neofusicoccum includes species with wide geographical and plant host distribution, some of them of economic importance. The genus currently comprises 27 species that are difficult to identify based on morphological features alone. Thus, species differentiation is based on phylogenetic species recognition using multigene genealogies. In this study, we characterised the mating type genes of Neofusicoccum species. Specific primers were designed to amplify and sequence MAT genes in several species and a PCR-based mating type diagnostic assay was developed. Homothallism was the predominant mating strategy among the species tested. Furthermore, the potential of mating type gene sequences for species delimitation was evaluated. Phylogenetic analyses were performed on both MAT genes and compared with multigene genealogies using sequences of the ribosomal internal transcribed spacer region, translation elongation factor 1-alpha and beta-tubulin. Phylogenies based on mating type genes could discriminate between the species analysed and are in concordance with the results obtained with the more conventional multilocus phylogenetic analysis approach. Thus, MAT genes represent a powerful tool to delimit cryptic species in the genus Neofusicoccum. (C) 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
author address [Lopes, Anabela; Alves, Artur] Univ Aveiro, CESAM, Biol Dept, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Phillips, Alan J. L.] Univ Lisbon, Fac Sci Biosyst & Integrat Sci Inst BioISI, P-1749016 Lisbon, Portugal
reprint address Alves, A (reprint author), Univ Aveiro, CESAM, Biol Dept, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address artur.alves@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788]; CESAM [UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638]; FCT Investigator Programme [IF/00835/2013]; PhD grant [SFRH/BD/85615/2012]
funding text This work was financed by European Funds through COMPETE and National Funds through the Portuguese Foundation for Science and Technology (FCT) to project ALIEN (PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788), CESAM (UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638), Artur Alves (FCT Investigator Programme - IF/00835/2013) anAnabela Lopes (PhD grant - SFRH/BD/85615/2012). We are thankful to Benedetto Linaldeddu (University of Sassari, Italy), Vincenzo Mondello (University of Palermo, Italy) and Akila Berraf-Tebbal (University of Blida, Algeria) for sharing some of their isolates.
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cited reference count 54
times cited 1
total times cited count (wos, bci, and cscd) 1
publisher city OXFORD
issn 1878-6146
29-character source abbreviation FUNGAL BIOL-UK
iso source abbreviation Fungal Biol.
publication date APR
year published 2017
volume 121
issue 4
special issue SI
beginning page 394
ending page 404
digital object identifier (doi) 10.1016/j.funbio.2016.08.011
page count 11
web of science category Mycology
subject category Mycology
document delivery number ES9AG
unique article identifier WOS:000399847700006
link http://dx.doi.org/10.1016/j.funbio.2016.08.011