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title Diversity and phylogeny of Neofusicoccum species occurring in forest and urban environments in Portugal
authors Lopes, A; Barradas, C; Phillips, AJL; Alves, A
author full name Lopes, A.; Barradas, C.; Phillips, A. J. L.; Alves, A.
title Diversity and phylogeny of Neofusicoccum species occurring in forest and urban environments in Portugal
nationality internacional
source MYCOSPHERE
language English
document type Article
author keywords Botryosphaeriaceae; endophytic; host-association; ornamentals; pathogenic
keywords plus SP-NOV; 1ST REPORT; NATIVE MYRTACEAE; RIBIS COMPLEX; SOUTH-AFRICA; BOTRYOSPHAERIACEAE; DIEBACK; EUCALYPTUS; CANKER; PARVUM
abstract A collection of Neofusicoccum isolates was obtained from a large number of plant species, showing dieback and canker symptoms, in forest and urban environments in Portugal. A total of 351 isolates was characterised by BOX-PCR fingerprinting to evaluate their overall genetic diversity. Representatives of each group identified in this analysis were selected for multilocus sequence analyses, using sequences of the ribosomal internal transcribed spacer region (ITS rDNA) and partial sequences of the translation elongation factor 1-alpha (tef1) and beta-tubulin (tub2). Phylogenetic analysis of multilocus sequence data identified five species within the collection of isolates, namely N. australe, N. eucalyptorum, N. kwambonambiense, N. luteum, and N. parvum. Of these N. australe and N. eucalyptorum were the most frequent representing the vast majority of the isolates. Several new fungus-host associations were established for all of the Neofusicoccum species found. Here we report for the first time the occurrence of N. eucalyptorum on a host outside the family Myrtaceae. The results of this study show that the genus Neofusicocccum appears to be common and widespread on a broad range of hosts representing a potential threat to susceptible plants. Additionally, ornamental plants in urban environments are shown to be hosts of a diverse assemblage of Neofusicoccum species.
author address [Lopes, A.; Barradas, C.; Alves, A.] Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Phillips, A. J. L.] Univ Lisbon, Biosyst & Integrat Sci Inst, Fac Sci, P-1749016 Lisbon, Portugal
reprint address Alves, A (reprint author), Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address artur.alves@ua.pt
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788, UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638, IF/00835/2013, SFRH/BD/85615/2012, SFRH/BD/77939/2011]
funding text This work was financed by European Funds through COMPETE and National Funds through the Portuguese Foundation for Science and Technology (FCT) to project ALIEN (PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788), CESAM (UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638), Artur Alves (FCT Investigator Programme - IF/00835/2013), Anabela Lopes (PhD grant - SFRH/BD/85615/2012) and Carla Barradas (PhD grant - SFRH/BD/77939/2011).
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cited reference count 52
publisher MYCOSPHERE PRESS
publisher city GUIYANG
publisher address GUIZHOU KEY LAB AGRIC BIOTECH, GUIZHOU ACAD AGRIC SCI, GUIYANG, 00000, PEOPLES R CHINA
issn 2077-7000
29-character source abbreviation MYCOSPHERE
iso source abbreviation Mycosphere
year published 2016
volume 7
issue 7
special issue SI
beginning page 906
ending page 920
digital object identifier (doi) 10.5943/mycosphere/si/1b/10
page count 15
web of science category Mycology
subject category Mycology
document delivery number EJ2LR
unique article identifier WOS:000393042000005
link http://dx.doi.org/10.5943/mycosphere/si/1b/10