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title Saccharospirillum correiae sp nov., an endophytic bacterium isolated from the halophyte Halimione portulacoides
authors Fidalgo, C; Rocha, J; Proenca, DN; Morais, PV; Alves, A; Henriques, I
author full name Fidalgo, Catia; Rocha, Jaqueline; Proenca, Diogo Neves; Morais, Paula Vasconcelos; Alves, Artur; Henriques, Isabel
title Saccharospirillum correiae sp nov., an endophytic bacterium isolated from the halophyte Halimione portulacoides
nationality internacional
language English
document type Article
author keywords Saccharospirillaceae; endophytic; salt marsh; halophytes; taxonomy
abstract A Gram-stain negative, oxidase-and catalase-positive, motile, aerobic, non-pigmented spirillum, designated CPA1(T), was isolated from The surface-sterilized tissues of a halophyte, Halimione portulacoides, collected from a salt marsh in Aveiro, Portugal. The isolate was mesophilic, facultatively alkaliphilic and halophilic, and grew between 18 and 42.5 degrees C (optimum 30 degrees C), from pH 5.0 to 11.5 (optimum 7.0-7.5), from 0.5 to 5% NaCl (w/v, optimum 2 %). Analysis of the 16S rRNA gene sequence showed that this strain belongs to the genus Saccharospirillum, as the highest sequence similarities were observed with Saccharospirillum impatiens EL-105(T) (96.46 %), Saccharospirillum salsuginis YIM-Y25(T) (96.32 %) and Saccharospirillum aestuarii IMCC 4453(T) (95.17 %). The next closest matches were with other genera and below 95.0 %. Phylogenetic analyses revealed that the strain forms a robust clade with other species of the genus Saccharospirillum. The main respiratory quinone was Q-8 and the major fatty acids were C-16 : 0 and summed feature 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c). The DNA G+ C content was 55.2 mol%. Molecular, physiological and biochemical differences between strain CPA1(T) and other type strains of species of the genus Saccharospirillum support the addition of this novel species to the genus, and the name Saccharospirillum correiae sp. nov. is proposed, with CPA1(T) (= CECT 9131(T) = LMG 29516(T)) as the type strain.
author address [Fidalgo, Catia; Rocha, Jaqueline; Alves, Artur; Henriques, Isabel] Univ Aveiro, Dept Biol, CESAM, Aveiro, Portugal; [Proenca, Diogo Neves; Morais, Paula Vasconcelos] Univ Coimbra, CEMUC, P-3030788 Coimbra, Portugal; [Morais, Paula Vasconcelos] Univ Coimbra, Dept Life Sci, FCTUC, P-3000456 Coimbra, Portugal
reprint address Alves, A (reprint author), Univ Aveiro, Dept Biol, CESAM, Aveiro, Portugal.
e-mail address artur.alves@ua.pt
orcid number Alves, Artur/0000-0003-0117-2958
funding agency and grant number European Funds (FEDER) through COMPETE; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER-019328]; FCT [UID/AMB/50017/2013- POCI-01-0145-FEDER-007638, IF/00835/2013, IF/00492/2013, SFRH/BPD/100721/2014, SFRH/BD/85423/2012]
funding text This work was supported by European Funds (FEDER) through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh (PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER-019328). FCT is acknowledged for financing to CESAM (UID/AMB/50017/2013- POCI-01-0145-FEDER-007638), Artur Alves (FCT Investigator Programme - IF/00835/2013), Isabel Henriques (FCT Investigator Programme - IF/00492/2013), Diogo Neves Proenca (postdoctoral grant SFRH/BPD/100721/2014) and Catia Fidalgo (PhD grant - SFRH/BD/85423/2012).
cited references Bowman JP, 2000, INT J SYST EVOL MICR, V50, P1861; Chen YG, 2009, INT J SYST EVOL MICR, V59, P1382, DOI 10.1099/ijs.0.003616-0; Choi A, 2011, INT J SYST EVOL MICR, V61, P487, DOI 10.1099/ijs.0.022996-0; da Costa MS, 2011, METHOD MICROBIOL, V38, P183, DOI 10.1016/B978-0-12-387730-7.00008-5; FELSENSTEIN J, 1981, J MOL EVOL, V17, P368, DOI 10.1007/BF01734359; Fidalgo C, 2016, ENVIRON SCI POLLUT R, V23, P10200, DOI 10.1007/s11356-016-6208-1; FITCH WM, 1971, SYST ZOOL, V20, P406, DOI 10.2307/2412116; Hall T.A., 1999, NUCL ACIDS S SER, V41, P95, DOI DOI 10.1021/BK-1999-0734.CH008; Kaksonen AH, 2006, APPL ENVIRON MICROB, V72, P3759, DOI 10.1128/AEM.72.5.3759-3762.2006; Kim OS, 2012, INT J SYST EVOL MICR, V62, P716, DOI 10.1099/ijs.0.038075-0; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; Labrenz M, 2003, INT J SYST EVOL MICR, V53, P653, DOI 10.1099/ijs.0.02406-0; Lane DJ, 1991, NUCL ACID TECHNIQUES, P115; McWilliam H, 2013, NUCLEIC ACIDS RES, V41, pW597, DOI 10.1093/nar/gkt376; MESBAH M, 1989, INT J SYST BACTERIOL, V39, P159; Proenca DN, 2014, INT J SYST EVOL MICR, V64, P1237, DOI 10.1099/ijs.0.053454-0; SAITOU N, 1987, MOL BIOL EVOL, V4, P406; STACKEBRANDT E, 1994, INT J SYST BACTERIOL, V44, P846; Tamura K, 2013, MOL BIOL EVOL, V30, P2725, DOI 10.1093/molbev/mst197; WAYNE LG, 1987, INT J SYST BACTERIOL, V37, P463
cited reference count 20
publisher city LONDON
issn 1466-5026
29-character source abbreviation INT J SYST EVOL MICR
iso source abbreviation Int. J. Syst. Evol. Microbiol.
publication date JUN
year published 2017
volume 67
issue 6
beginning page 2026
ending page 2030
digital object identifier (doi) 10.1099/ijsem.0.001914
page count 5
web of science category Microbiology
subject category Microbiology
document delivery number FA5SL
unique article identifier WOS:000405503800065
link http://dx.doi.org/10.1099/ijsem.0.001914