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title Zunongwangia endophytica sp nov., an endophyte isolated from the salt marsh plant, Halimione portulacoides, and emended description of the genus Zunongwangia
authors Fidalgo, C; Martins, R; Proenca, DN; Morais, PV; Alves, A; Henriques, I
author full name Fidalgo, Catia; Martins, Ricardo; Proenca, Diogo Neves; Morais, Paula V.; Alves, Artur; Henriques, Isabel
title Zunongwangia endophytica sp nov., an endophyte isolated from the salt marsh plant, Halimione portulacoides, and emended description of the genus Zunongwangia
nationality internacional
source INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
language English
document type Article
author keywords Flavobacteriaceae; endophytic; salt marsh; halophytes; taxonomy
keywords plus FLAVOBACTERIACEAE; SEQUENCES; FAMILY; TREES
abstract Taxonomical analyses were performed on strain CPA58(T), a novel isolate obtained from surface-sterilized aboveground tissues of the halophyte Halimione portulacoides, collected from a salt marsh in Ria de Aveiro, Portugal. The strain was Gram-stain-negative, rod-shaped, oxidase-negative and catalase-positive. Optimal growth was observed at 26 degrees C, at pH 6-8 and in the presence of 2 to 3% (w/v) NaCl. Phylogenetic analyses, based on the 16S rRNA gene sequence, showed that strain CPA58(T) belongs to the genus Zunongwangia, with highest sequence similarities to both Zunongwangia profunda SMA87 T and Zunongwangia mangrovi P2E16(T) (96.5 %), followed by Zunongwangia atlantica 22II14-10F7(T) (95.9 %). The principal fatty acids were iso-C15 : 0, summed feature 3 (C-16 : 1 omega 7c and/or iso-C-15 : 0 2-OH) and iso-C-17 : 0 3-OH. The major respiratory quinone was MK-6 and the DNA G+ C content was 35.1 mol%. Phylogenetic and chemotaxonomic analyses clearly placed strain CPA58(T) in the genus Zunongwangia. However, 16S rRNA gene sequence analysis showed that the threshold for same species relatedness was not surpassed, and biochemical tests revealed diagnostic characteristics that differentiated this strain from other type strains of species of the genus Zunongwangia. Overall, the analyses showed that strain CPA58(T) represents a novel species within the genus Zunongwangia, for which the name Zunongwangia endophytica sp. nov. is proposed, with the type strain CPA58(T) (= CECT 9128(T) = LMG 29517(T)).
author address [Fidalgo, Catia; Martins, Ricardo; Alves, Artur; Henriques, Isabel] Univ Aveiro, Dept Biol, CESAM, Aveiro, Portugal; [Proenca, Diogo Neves; Morais, Paula V.] Univ Coimbra, CEMUC, P-3030788 Coimbra, Portugal; [Morais, Paula V.] Univ Coimbra, Dept Life Sci, FCTUC, P-3000456 Coimbra, Portugal
reprint address Alves, A (reprint author), Univ Aveiro, Dept Biol, CESAM, Aveiro, Portugal.
e-mail address artur.alves@ua.pt
researcherid number Alves, Artur/C-3645-2008; Henriques, Isabel/B-1667-2008
orcid number Alves, Artur/0000-0003-0117-2958; Henriques, Isabel/0000-0001-7717-4939; Neves Proenca, Diogo/0000-0002-1284-9082
funding agency and grant number European Funds (FEDER) through COMPETE; Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh [PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER-019328]; Portuguese Foundation for Science and Technology (FCT) within research unit CESAM [UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638]; FCT [IF/00835/2013, IF/00492/2013, SFRH/BPD/100721/2014, SFRH/BD/85423/2012]
funding text This work was supported by European Funds (FEDER) through COMPETE and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh (PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER-019328) and research unit CESAM (UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638). FCT is acknowledged for financing to Artur Alves (FCT Investigator Programme - IF/00835/2013), Isabel Henriques (FCT Investigator Programme - IF/00492/2013), Diogo Proenca (post doc grant - SFRH/BPD/100721/2014) and Catia Fidalgo (PhD grant - SFRH/BD/85423/2012).
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cited reference count 20
publisher MICROBIOLOGY SOC
publisher city LONDON
publisher address CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND
issn 1466-5026
29-character source abbreviation INT J SYST EVOL MICR
iso source abbreviation Int. J. Syst. Evol. Microbiol.
publication date AUG
year published 2017
volume 67
issue 8
beginning page 3004
ending page 3009
digital object identifier (doi) 10.1099/ijsem.0.002069
subject category 6
document delivery number Microbiology
unique article identifier Microbiology
link http://dx.doi.org/10.1099/ijsem.0.002069