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title Altererythrobacter halimionae sp nov and Altererythrobacter endophyticus sp nov., two endophytes from the salt marsh plant Halimione portulacoides
authors Fidalgo, C; Rocha, J; Martins, R; Proenca, DN; Morais, PV; Henriques, I; Alves, A
author full name Fidalgo, Catia; Rocha, Jaqueline; Martins, Ricardo; Proenca, Diogo Neves; Morais, Paula V.; Henriques, Isabel; Alves, Artur
title Altererythrobacter halimionae sp nov and Altererythrobacter endophyticus sp nov., two endophytes from the salt marsh plant Halimione portulacoides
nationality internacional
source INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY
language English
document type Article
author keywords Erythrobacteraceae; endophytic; halophytes; taxonomy
keywords plus EMENDED DESCRIPTION; ALPHAPROTEOBACTERIA; SEQUENCES; SEAWATER; TREES
abstract Two Gram-negative, rod-shaped, motile bacterial strains, named CPA5(T) and BR75(T), were isolated from the halophyte Halimione portulacoides. Both presented optimum growth at 30 degrees C , pH 7.0-7.5 and 1-2% NaCl (w/v) for strain CPA5(T), and pH 7.5-8.0 and 2% NaCl (w/v) for strain BR75(T). Phylogenetic analyses based on 16S rRNA gene sequences affiliated both strains to the genus Altererythrobacter. CPA5(T) presented highest 16S rRNA gene sequence similarity with Altererythrobacter aestuarii KYW147(T) (96.5 %), followed by Altererythrobacter namhicola KYW48(T) (95.9 %), Novosphingobium indicum H25(T) (95.6 %) and Altererythrobacter oceanensis Y2(T) (95.5 %). BR75(T) displayed highest similarity with Altererythrobacter marensis MSW-14(T) (96.5 %), followed by Altererythrobacter xinjiangensis S3-63(T), Altererythrobacter luteolus SW-109(T) and Altererythrobacter indicus MSSRF26(T) (96.1 %). Neither strain contained Bacteriochlorophyll a. The main fatty acids observed for CPA5(T) were C-17 : 1 omega 6c and summed features 3 (C-16 : 1 omega 7c and/or iso-C-15 (:) (0) 2-OH) and 8 (C-18 : 1 omega 7c and/or C-18 : 1 omega 6c). The latter summed feature was the dominant fatty acid observed for strain BR75(T) as well. The major polar lipids were phosphatidylethanolamine, unidentified phospholipids and unidentified glycolipids for both strains. The predominant ubiquinone was Q-10 for both strains, and the DNA G+C contents were 63.4 mol% and 58.3 mol% for CPA5(T) and BR75(T), respectively. Based on phenotypic and genotypic results, both strains represent novel species belonging to the genus Altererythrobacter for which the names Altererythrobacter halimionae sp. nov. (type strain CPA5(T)=CECT 9130(T)=LMG 29519(T)) and Altererythrobacter endophyticus sp. nov (type strain BR75(T)=CECT 9129(T)=LMG 29518(T)) are proposed.
author address [Fidalgo, Catia; Rocha, Jaqueline; Martins, Ricardo; Henriques, Isabel; Alves, Artur] Univ Aveiro, Dept Biol, CESAM, Aveiro, Portugal; [Proenca, Diogo Neves; Morais, Paula V.] Univ Coimbra, CEMUC, P-3030788 Coimbra, Portugal; [Morais, Paula V.] Univ Coimbra, Dept Life Sci, FCTUC, P-3000456 Coimbra, Portugal
reprint address Henriques, I (reprint author), Univ Aveiro, Dept Biol, CESAM, Aveiro, Portugal.
e-mail address ihenriques@ua.pt
researcherid number Henriques, Isabel/B-1667-2008; Alves, Artur/C-3645-2008
orcid number Henriques, Isabel/0000-0001-7717-4939; Alves, Artur/0000-0003-0117-2958; Neves Proenca, Diogo/0000-0002-1284-9082
funding agency and grant number European funds (FEDER) through COMPETE; national funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh [PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER-019328]; FCT [UID/AMB/50017/2013, IF/00835/2013, IF/00492/2013, SFRH/BD/85423/2012, SFRH/BPD/100721/2014]
funding text This work was supported by European funds (FEDER) through COMPETE and by national funds through the Portuguese Foundation for Science and Technology (FCT) within project PhytoMarsh (PTDC/AAC-AMB/118873/2010 - FCOMP-01-0124-FEDER-019328). FCT is acknowledged for financing to CESAM (UID/AMB/50017/2013), A. A. (FCT Investigator Programme - IF/00835/2013), I. H. (FCT Investigator Programme - IF/00492/2013), C. F. (PhD grant - SFRH/BD/85423/2012) and D. P. (Post Doc grant - SFRH/BPD/100721/2014).
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publisher MICROBIOLOGY SOC
publisher city LONDON
publisher address CHARLES DARWIN HOUSE, 12 ROGER ST, LONDON WC1N 2JU, ERKS, ENGLAND
issn 1466-5026
29-character source abbreviation INT J SYST EVOL MICR
iso source abbreviation Int. J. Syst. Evol. Microbiol.
publication date AUG
year published 2017
volume 67
issue 8
beginning page 3057
ending page 3062
digital object identifier (doi) 10.1099/ijsem.0.002079
subject category 6
document delivery number Microbiology
unique article identifier Microbiology
link http://dx.doi.org/10.1099/ijsem.0.002079