HOME  »  Members  »  Collaborators  »  PhD Students/Fellows
title Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms
authors Araujo, S; Silva, IAT; Tacalo, M; Patinha, C; Alves, A; Henriques, I
author full name Araujo, Susana; Silva, Isabel A. T.; Tacalo, Marta; Patinha, Carla; Alves, Artur; Henriques, Isabel
title Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms
nationality internacional
language English
document type Article
author keywords Aquatic compartments; Antibiotic resistance; Vegetables contamination; Irrigation water; Escherichia colt
abstract This study aimed to characterize Escherichia coli present in irrigation water and vegetables from 16 household farms. Isolates were obtained from 50% of water (n = 210 isolates) and 38% of vegetable samples (n = 239). Phylogroups B1 (56% of isolates) and A (22%) were the most prevalent both in water and vegetables. Diarrheagenic strains were detected in vegetables. Irrespective of the source (i.e. water or vegetables), the most common antibiotic resistance was against streptomycin (89% resistant isolates) and tetracycline (24%). Common acquired genes (e.g. blar(TEM), tetA, tetB) were found in isolates from both sources. Class I integrons were detected in water (arrays dfrA1-aadA1 and dfr16-blaPlb-aadA2-ereA) and vegetables (unknown arrays). intI2 was detected in water (dfrA1-sat2-aadA1). Plasmids were detected in 14 isolates (IncFIC, IncFIB, IncFrep, Inch in both samples; IncY in vegetables). Plasmids from seven isolates were transferrable by conjugation, conferring resistance to antibiotics to the recipient strain. Multidrug-resistant (MDR) strains were isolated from water (12% of the unique isolates) and vegetables (21%). Predominant sequence types (STs) among MDR isolates were ST10, ST297 and ST2522. In some cases, the same STs and identical clones (as showed by rep-PCR typing) were detected in water and vegetables, suggesting cross-contamination. This study identified several risk factors in E. coil isolates from vegetables and irrigation water, raising health concerns. Also, results suggest that irrigation groundwater constitutes a source of E. coli that may enter the food chain through vegetables ingestion.
author address [Araujo, Susana; Silva, Isabel A. T.; Tacalo, Marta; Alves, Artur; Henriques, Isabel] Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Patinha, Carla] Univ Aveiro, Geosci Dept, Geobiotec, Campus Santiago, P-3810193 Aveiro, Portugal
reprint address Henriques, I (reprint author), Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address ihenriques@ua.pt
researcherid number Alves, Artur/C-3645-2008; Tacao, Marta/C-9785-2009; Henriques, Isabel/B-1667-2008
orcid number Alves, Artur/0000-0003-0117-2958; Tacao, Marta/0000-0001-7965-4928; Henriques, Isabel/0000-0001-7717-4939; Patinha, Carla/0000-0001-6006-344X; Araujo, Susana/0000-0002-2902-9873
funding agency and grant number National Funds from FCT Fundacao para a Ciencia e a Tecnologia [JPI/0002/2013]; FCT [UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638]; Artur Alves (FCT Investigator Programme) [IF/00492/2013]; Isabel Henriques (FCT Investigator Programme) [IF/00492/2013]; Marta Tacao [SFRH/BPD/114855/2016]; Susana Aratijo [SFRH/BD/52573/2014]
funding text We also thank Marta Alves for the support during sampling. This work was supported by National Funds from FCT Fundacao para a Ciencia e a Tecnologia - through project Water JPI/0002/2013
cited references Abadias M, 2008, INT J FOOD MICROBIOL, V123, P121, DOI 10.1016/j.ijfoodmicro.2007.12.013; Ahmed D, 2013, J APPL MICROBIOL, V114, P1223, DOI 10.1111/jam.12109; Aizawa J, 2014, J ANTIMICROB CHEMOTH, V69, P2866, DOI 10.1093/jac/dku218; Altschul SF, 1997, NUCLEIC ACIDS RES, V25, P3389, DOI 10.1093/nar/25.17.3389; Amezquita-Montes Z, 2015, FOODBORNE PATHOG DIS, V12, P454, DOI 10.1089/fpd.2014.1881; Aranda KRS, 2007, FEMS MICROBIOL LETT, V267, P145, DOI 10.1111/j.1574-6968.2006.00580.x; Araujo S, 2014, SCI TOTAL ENVIRON, V470, P84, DOI 10.1016/j.scitotenv.2013.09.075; Bennett PM, 2008, BRIT J PHARMACOL, V153, pS347, DOI 10.1038/sj.bjp.0707607; Berger CN, 2010, ENVIRON MICROBIOL, V12, P2385, DOI 10.1111/j.1462-2920.2010.02297.x; Beuchat LR, 1996, J FOOD PROTECT, V59, P204, DOI 10.4315/0362-028X-59.2.204; Beuchat LR, 1997, EMERG INFECT DIS, V3, P459, DOI 10.3201/eid0304.970407; Beuchat L. R., 2002, MICROBES INFECT; Boehme S, 2004, MOL NUTR FOOD RES, V48, P522, DOI 10.1002/mnfr.200400030; Carattoli A, 2005, J MICROBIOL METH, V63, P219, DOI 10.1016/j.mimet.2005.03.018; Chah KF, 2010, J BASIC MICROB, V50, pS18, DOI 10.1002/jobm.200900325; Chattaway MA, 2014, EMERG INFECT DIS, V20, P1935, DOI 10.3201/eid2011.131845; Clermont O, 2013, ENV MICROBIOL REP, V5, P58, DOI 10.1111/1758-2229.12019; da Sande Diregao-Geral, 2014, PREV CONTR INF RES A; De Roever C, 1999, FOOD CONTROL, V10, P117; Diregao-Geral De Agriculture, 2003, SIT AGR PORT; ECDC EFSA EMA, 2015, EFSA J; Edelstein M., 2014, PLOS CURR OUTBREAKS, P1; EFSA BIOHAZ Panel, 2013, EFSA J; Elmadfa I, 2009, EUROPEAN NUTR HLTH R; European Commission, 2008, NEW GROUNDW DIR CONS; FAO/WHO (Food and Agriculture Organization/World Health Organization), 2008, MICR HAZ FRESH LEAF; FRIESEMA I., 2008, EUROSURVEILLANCE, V13, P1; Giufre M, 2012, J ANTIMICROB CHEMOTH, V67, P860, DOI 10.1093/jac/dkr565; Hamilton-Miller JMT, 2001, INT J ANTIMICROB AG, V18, P81, DOI 10.1016/S0924-8579(01)00353-3; Hassan SA, 2011, FOODBORNE PATHOG DIS, V8, P1011, DOI 10.1089/fpd.2010.0805; Henriques IS, 2006, RES MICROBIOL, V157, P938, DOI 10.1016/j.resmic.2006.09.003; Hilborn ED, 1999, ARCH INTERN MED, V159, P1758, DOI 10.1001/archinte.159.15.1758; Holvoet K, 2013, APPL ENVIRON MICROB, V79, P6677, DOI 10.1128/AEM.01995-13; Jahanbalchsh S., 2016, INT J ANTIMICROB AGE; Jung Y., 2014, MICROB BIOTECHNOL; Kaper JB, 2004, NAT REV MICROBIOL, V2, P123, DOI 10.1038/nrmicro818; Koo HJ, 2012, WORLD J MICROB BIOT, V28, P1813, DOI 10.1007/s11274-011-0954-5; Laroche E, 2009, FEMS MICROBIOL ECOL, V68, P118, DOI 10.1111/j.1574-6941.2009.00655.x; LeJeune JT, 2001, APPL ENVIRON MICROB, V67, P3053, DOI 10.1128/AEM.67.7.3053-3057.2001; Lupo A, 2012, FRONT MICROBIOL, V3, DOI 10.3389/fmicb.2012.00018; Marti R, 2013, APPL ENVIRON MICROB, V79, P5701, DOI 10.1128/AEM.01682-13; Miraldo C. M., 2007, ESTUDO CONTAMINACAO; Moran RA, 2015, PLASMID, V80, P24, DOI 10.1016/j.plasmid.2015.03.005; Mosquito S., 2015, SCI WORLD J, V2015, P1; Moura A, 2007, J ANTIMICROB CHEMOTH, V60, P1243, DOI 10.1093/jac/dkm340; Moura A, 2014, FRONT MICROBIOL, V5, DOI 10.3389/fmicb.2014.00419; Moura A, 2012, FEMS MICROBIOL LETT, V330, P157, DOI 10.1111/j.1574-6968.2012.02544.x; Moura A, 2012, RES MICROBIOL, V163, P92, DOI 10.1016/j.resmic.2011.10.010; Moura A, 2009, BIOINFORMATICS, V25, P1096, DOI 10.1093/bioinformatics/btp105; National Center for Emerging and Zoonotic Infectious Diseases, 2011, CDC EST FOODB ILLN U; Nataro JP, 1998, CLIN MICROBIOL REV, V11, P142; Osterblad M, 1999, J ANTIMICROB CHEMOTH, V43, P503, DOI 10.1093/jac/43.4.503; Pannaraj PS, 2015, PEDIATR INFECT DIS J, V34, P11, DOI 10.1097/INF.0000000000000471; Paton AW, 1998, J CLIN MICROBIOL, V36, P598; Pereira A, 2013, SCI TOTAL ENVIRON, V461, P65, DOI 10.1016/j.scitotenv.2013.04.067; Pomerleau J, 2006, PUBLIC HEALTH NUTR, V9, P575, DOI 10.1079/PHN2005910; Potron A, 2013, EUROSURVEILLANCE, V18, P9, DOI 10.2807/1560-7917.ES2013.18.31.20549; Richardson HY, 2009, WATER RES, V43, P2159, DOI 10.1016/j.watres.2009.02.035; Rodrigues C, 2015, FUTURE MICROBIOL, V10, P1125, DOI 10.2217/fmb.15.38; Schwaiger K, 2011, INT J FOOD MICROBIOL, V148, P191, DOI 10.1016/j.ijfoodmicro.2011.06.001; Shahada F, 2013, POULTRY SCI, V92, P1641, DOI 10.3382/ps.2012-02934; Stiderstrom A., 2008, FOODBORNE PATHOG DIS, V5, P339; Szmolka A, 2013, FRONT MICROBIOL, V4, DOI 10.3389/fmicb.2013.00258; Tacao M, 2015, MICROB DRUG RESIST, V21, P497, DOI 10.1089/mdr.2015.0072; Tacao M, 2014, WATER RES, V48, P100, DOI 10.1016/j.watres.2013.09.021; Tacao M, 2012, APPL ENVIRON MICROB, V78, P4134, DOI 10.1128/AEM.00359-12; Valverde A, 2009, ANTIMICROB AGENTS CH, V53, P5204, DOI 10.1128/AAC.01706-08; VERSALOVIC J, 1991, NUCLEIC ACIDS RES, V19, P6823, DOI 10.1093/nar/19.24.6823; Viswanathan P, 2001, INT J HYG ENVIR HEAL, V203, P205, DOI 10.1078/S1438-4639(04)70030-9; von Wintersdorff CJH, 2016, FRONT MICROBIOL, V7, DOI 10.3389/fmicb.2016.00173; Wirth T, 2006, MOL MICROBIOL, V60, P1136, DOI 10.1111/j.1365-2958.2006.05172.x; Zurfluh K, 2015, APPL ENVIRON MICROB, V81, P3115, DOI 10.1128/AEM.00258-15
cited reference count 72
publisher city AMSTERDAM
publisher address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
issn 0168-1605
29-character source abbreviation INT J FOOD MICROBIOL
iso source abbreviation Int. J. Food Microbiol.
publication date SEP 18
year published 2017
volume 257
beginning page 192
ending page 200
digital object identifier (doi) 10.1016/j.ijfoodmicro.2017.06.020
subject category 9
document delivery number Food Science & Technology; Microbiology
unique article identifier Food Science & Technology; Microbiology
link https://doi.org/10.1016/j.ijfoodmicro.2017.06.020