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title Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms
authors Araujo, S; Silva, IAT; Tacalo, M; Patinha, C; Alves, A; Henriques, I
author full name Araujo, Susana; Silva, Isabel A. T.; Tacalo, Marta; Patinha, Carla; Alves, Artur; Henriques, Isabel
title Characterization of antibiotic resistant and pathogenic Escherichia coli in irrigation water and vegetables in household farms
nationality internacional
source INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
language English
document type Article
author keywords Aquatic compartments; Antibiotic resistance; Vegetables contamination; Irrigation water; Escherichia colt
keywords plus LACTAMASE-PRODUCING ENTEROBACTERIACEAE; ANTIMICROBIAL RESISTANCE; SALAD VEGETABLES; AQUATIC SYSTEMS; GENE CASSETTES; BACTERIA; PREVALENCE; INTEGRONS; DIVERSITY; PLASMIDS
abstract This study aimed to characterize Escherichia coli present in irrigation water and vegetables from 16 household farms. Isolates were obtained from 50% of water (n = 210 isolates) and 38% of vegetable samples (n = 239). Phylogroups B1 (56% of isolates) and A (22%) were the most prevalent both in water and vegetables. Diarrheagenic strains were detected in vegetables. Irrespective of the source (i.e. water or vegetables), the most common antibiotic resistance was against streptomycin (89% resistant isolates) and tetracycline (24%). Common acquired genes (e.g. blar(TEM), tetA, tetB) were found in isolates from both sources. Class I integrons were detected in water (arrays dfrA1-aadA1 and dfr16-blaPlb-aadA2-ereA) and vegetables (unknown arrays). intI2 was detected in water (dfrA1-sat2-aadA1). Plasmids were detected in 14 isolates (IncFIC, IncFIB, IncFrep, Inch in both samples; IncY in vegetables). Plasmids from seven isolates were transferrable by conjugation, conferring resistance to antibiotics to the recipient strain. Multidrug-resistant (MDR) strains were isolated from water (12% of the unique isolates) and vegetables (21%). Predominant sequence types (STs) among MDR isolates were ST10, ST297 and ST2522. In some cases, the same STs and identical clones (as showed by rep-PCR typing) were detected in water and vegetables, suggesting cross-contamination. This study identified several risk factors in E. coil isolates from vegetables and irrigation water, raising health concerns. Also, results suggest that irrigation groundwater constitutes a source of E. coli that may enter the food chain through vegetables ingestion.
author address [Araujo, Susana; Silva, Isabel A. T.; Tacalo, Marta; Alves, Artur; Henriques, Isabel] Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Patinha, Carla] Univ Aveiro, Geosci Dept, Geobiotec, Campus Santiago, P-3810193 Aveiro, Portugal
reprint address Henriques, I (reprint author), Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address ihenriques@ua.pt
researcherid number Alves, Artur/C-3645-2008; Tacao, Marta/C-9785-2009; Henriques, Isabel/B-1667-2008
orcid number Alves, Artur/0000-0003-0117-2958; Tacao, Marta/0000-0001-7965-4928; Henriques, Isabel/0000-0001-7717-4939; Patinha, Carla/0000-0001-6006-344X; Araujo, Susana/0000-0002-2902-9873
funding agency and grant number National Funds from FCT Fundacao para a Ciencia e a Tecnologia [JPI/0002/2013]; FCT [UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638]; Artur Alves (FCT Investigator Programme) [IF/00492/2013]; Isabel Henriques (FCT Investigator Programme) [IF/00492/2013]; Marta Tacao [SFRH/BPD/114855/2016]; Susana Aratijo [SFRH/BD/52573/2014]
funding text We also thank Marta Alves for the support during sampling. This work was supported by National Funds from FCT Fundacao para a Ciencia e a Tecnologia - through project Water JPI/0002/2013
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cited reference count 72
publisher ELSEVIER SCIENCE BV
publisher city AMSTERDAM
publisher address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
issn 0168-1605
29-character source abbreviation INT J FOOD MICROBIOL
iso source abbreviation Int. J. Food Microbiol.
publication date SEP 18
year published 2017
volume 257
beginning page 192
ending page 200
digital object identifier (doi) 10.1016/j.ijfoodmicro.2017.06.020
subject category 9
document delivery number Food Science & Technology; Microbiology
unique article identifier Food Science & Technology; Microbiology
link https://doi.org/10.1016/j.ijfoodmicro.2017.06.020