Toxicology & Cytometry Laboratory (TCL)
Published in 13/4/2020
The Toxicology and Cytometry Lab is focused on the understanding of toxicity mechanisms of the main classes of contaminants (e.g. metals, PAHs, pesticides, nanomaterials - NM) and biotoxins, in a wide range of organisms, from bacteria, dinoflagellates, crustacean and fish up to mammalian/human systems. Adopting in vitro, ex vivo and in vivo approaches, the processes addressed encompass oxidative stress, biotransformation, endocrine disruption, neurotoxicity and genotoxicity, keeping in view an integrative interpretation of toxicity pathways.
The Lab includes researchers with multiple levels of expertise, with emphasis on Flow cytometry (FCM). FCM is a powerful tool in toxicology a with broad range of applications. The team is currently focused on: i) Evaluation of population dynamics under contexts of environmental contamination; ii) Assessment of toxicity of NM towards human and animal cells by evaluating a panel of endpoints related with cytotoxic, genotoxic and inflammation responses (e.g. cell viability, NM uptake, apoptosis, oxidative imbalances, mitochondrial function, cell cycle dynamics and quantification of cytokines).