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title Automatic counter and characterization in ecotoxicology assays
authors Abreu S.N., Oliveira e Silva M., Martins R.E. & Soares A.M.V.M.
title Automatic counter and characterization in ecotoxicology assays
nationality internacional
abstract One of the most worldwide bioassays used in ecotoxicology to evaluate effluents and contaminated waters is performed with Daphnia, where most of the acute tests require a repetitive and time consuming counting procedure, aiming endpoints such as “reproduction”, “immobility” or “lethality”. We present a new technology for counting organisms embedded in a solution (e.g. Daphnia in ecotoxicology assays) based on organism serialization and conduction along a tubular cell passing through an electronic optical detector. The generated analogue optical signal is then processed by a computational system to perform the organism counting and, eventually, a chromatic classification (to infer its morbidity/mortality). With this technology it is possible to adjust the light chromatic emission, aiming an enhanced organism/solution contrast for counting purposes or, alternatively, to filter away the characteristic green colour of the organism algae food (enhancing the quality of the chromatic classification of the organism). It is even possible to expose the solution to non-visible light spectrum (e.g. infra-red aiming eventually a more precise morbidity/mortality classification). This technology allows a real-time counting of organisms present in a solution with a fully automatized procedure, thus avoiding current error-prone “manual” counting and operator fatigue; thus, enhancing data quality and significantly reducing global procedure time. Chromatic classification of organisms may also allow better data results as it will give objective indication of each organism morbidity/mortality state. It is also possible to simultaneously process several solutions in a multi-counter realization of this apparatus.
publication date 06-May-2015
year published 2015