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title Cholinesterase activity in the caddisfly Sericostoma vittatum: Biochemical enzyme characterization and in vitro effects of insecticides and psychiatric drugs
authors Pestana, J.L.T.; Novais, S.C.; Lemos, M.F.L.; Soares, A.M.V.M.
author full name Pestana, Joao L. T.; Novais, Sara C.; Lemos, Marco F. L.; Soares, Amadeu M. V. M.
title Cholinesterase activity in the caddisfly Sericostoma vittatum: Biochemical enzyme characterization and in vitro effects of insecticides and psychiatric drugs
nationality internacional
source Ecotoxicology and Environmental Safety
language English
document type Article
author keywords Cholinesterase characterization; Psychiatric pharmaceuticals; Sericostoma vittatum; Carbaryl; Chlorantraniliprole
keywords plus ACETYLCHOLINESTERASE ACTIVITY; ORGANOPHOSPHATE INSECTICIDE; LARVAE TRICHOPTERA; ESTUARINE FISH; DAPHNIA-MAGNA; INHIBITION; BIOMARKERS; EXPOSURE; HYDROPSYCHIDAE; CARBAMAZEPINE
abstract Sericostoma vittatum is a caddisfly species, endemic to the Iberian Peninsula, proposed as a biomonitor species for lotic ecosystems. Since inhibition of choliriesterases' (ChE) activity has been used to evaluate the exposure of macroinvertebrates to organophosphates and carbamate pesticides, this work intended to characterize the ChE present in this species so their activity can be used as a potential biomarker of exposure. Biochemical and pharmacological properties of ChE were characterized in this caddisfly species using different substrates (acetylthiocholine iodide, propionylthiocholine iodide, and butyrylthiocholine iodide) and selective inhibitors (eserine sulfate, BW284c51, and iso-OMPA). Also, the in vitro effects of two insecticides (carbaryl and chlorantraniliprole) and two psychiatric drugs (fluoxetine and carbamazepine) on ChE activity were investigated. The results suggest that S. vittatum possess mainly AChE able to hydrolyze both substrates acetylthiocholine and propionylthiocholine since: (1) it hydrolyzes the substrate acetylthiocholine and propionylcholine at similar rates and butyrylthiocholine at a much lower rate; (2) it is highly sensitive to eserine sulfate and BW284c51, but not to iso-OMPA; and (3) its activity is inhibited by excess of substrate, a characteristic of typical AChE. in vitro inhibitions were observed only for carbaryl exposure while exposure to chlorantraniliprole and to relevant environmental concentrations of psychiatric drugs did not cause any significant effect on AChE activity. This study suggests that AChE activity in caddisflies can indeed be used to discriminate the effects of specific insecticides in monitoring programs. The use of non-target species such as caddisflies in ecotoxicological research in lotic ecosystems is also discussed. (C) 2014 Elsevier Inc. All rights reserved.
author address [Pestana, Joao L. T.; Novais, Sara C.; Lemos, Marco F. L.; Soares, Amadeu M. V. M.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Pestana, Joao L. T.; Novais, Sara C.; Lemos, Marco F. L.; Soares, Amadeu M. V. M.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Novais, Sara C.; Lemos, Marco F. L.] Inst Politecn Leiria, ESTM, P-2520641 Peniche, Portugal; [Novais, Sara C.; Lemos, Marco F. L.] Inst Politecn Leiria, GIRM, P-2520641 Peniche, Portugal
reprint address Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address jpestana@ua.pt; sara.novais@ipleiria.pt; marco.lemos@ipleiria.pt; asoares@ua.pt
funding text Financial support for this work was provided by
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cited reference count 56
publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
publisher city SAN DIEGO
publisher address 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
issn 0147-6513
iso source abbreviation ECOTOX ENVIRON SAFE
publication date Ecotox. Environ. Safe.
year published 2014
volume 104
beginning page 263
ending page 268
digital object identifier (doi) 10.1016/j.ecoenv.2014.03.012
page count 6
web of science category Environmental Sciences; Toxicology
subject category Environmental Sciences & Ecology; Toxicology
unique article identifier AH3FS
link http://dx.doi.org/10.1016/j.ecoenv.2014.03.012