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title Behavioural responses of freshwater planarians after short-term exposure to the insecticide chlorantraniliprole
authors Rodrigues, ACM; Henriques, JF; Domingues, I; Golovko, O; Zlabek, V; Barata, C; Soares, AMVM; Pestana, JLT
author full name Rodrigues, Andreia C. M.; Henriques, Jorge F.; Domingues, Ines; Golovko, Oksana; Zlabek, Vladimir; Barata, Carlos; Soares, Amadeu M. V. M.; Pestana, Joao L. T.
title Behavioural responses of freshwater planarians after short-term exposure to the insecticide chlorantraniliprole
nationality internacional
source AQUATIC TOXICOLOGY
language English
document type Article
author keywords Feeding activity; Locomotion; Automated video tracking system; Dugesia subtentaculata; Anthranilic diamides
keywords plus ANTHRANILIC DIAMIDE INSECTICIDE; POLYCELIS-FELINA; CHIRONOMUS-RIPARIUS; LOCOMOTOR-ACTIVITY; DIPTERA-CULICIDAE; RISK-ASSESSMENT; GAMMARUS-PULEX; MUSCLE-FIBERS; END-POINT; TOXICITY
abstract Recent advances in video tracking technologies provide the tools for a sensitive and reproducible analysis of invertebrate activity under stressful conditions nurturing the field of behavioural ecotoxicology. This study aimed to evaluate behavioural responses of the freshwater planarian Dugesia subtentaculata exposed to a model compound, chlorantraniliprole (CAP). This compound is an anthranilic diamide insecticide and due to its neurotoxic action can, at low concentrations, impair behaviour of exposed organisms. Behavioural endpoints measured included feeding and locomotor activities. Feeding responses were based on planarian predatory behaviour using Chironomus riparius larvae as prey. Locomotion was measured by the traditional planarian locomotor velocity (pLMV) assay and additionally using an automated video tracking system using a Zebrabox (R) (Viewpoint, France) device. While feeding and pLMV were significantly impaired at 131.7 mu g/L CAP, the video tracking system showed that total distance covered by planarians was significantly reduced at concentrations as low as 26.2 mu g/L CAP. Our results show that more advanced automated video recording systems can be used in the development of sensitive bioassays allowing a reliable, time- and cost-effective quantification of behaviour in aquatic invertebrates. Due to their ecological relevance, behavioural responses should not be disregarded in risk assessment strategies and we advocate the suitability of planarians as suitable organisms for behavioural ecotoxicological studies. (C) 2015 Elsevier B.V. All rights reserved.
author address [Rodrigues, Andreia C. M.; Henriques, Jorge F.; Domingues, Ines; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Rodrigues, Andreia C. M.; Henriques, Jorge F.; Domingues, Ines; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, CESAM, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Golovko, Oksana; Zlabek, Vladimir] Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Zatisi 728-2, Vodnany 38925, Czech Republic; [Rodrigues, Andreia C. M.; Barata, Carlos] CSIC, IDAEA, Dept Environm Chem, Jordi Girona 18-26, ES-08034 Barcelona, Spain
reprint address Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.; Pestana, JLT (reprint author), Univ Aveiro, CESAM, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address jpestana@ua.pt
funding agency and grant number Portuguese Science Foundation (FCT) [CESAM: UID/AMB/50017/2013]; COMPETE program (Programa Operacional Fatores de Competitividade, FEDER component); FCT-Fundacao para a Ciencia e Tecnologia [FCOMP-01-0124-FEDER-019380, PTDC/AAC-AMB/119433/2010]; FCT; POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) [SFRH/BPD/45342/2008, SFRH/BPD/90521/2012, SFRH/BD/79424/2011]; Ministry of Education, Youth and Sports of the Czech Republic [CZ.1.05/2.1.00/01.0024, LO1205]
funding text This work was supported by the Portuguese Science Foundation (FCT) through CESAM: UID/AMB/50017/2013. We are thankful for the financial support of COMPETE program (Programa Operacional Fatores de Competitividade, FEDER component) and by National funding through FCT-Fundacao para a Ciencia e Tecnologia within the research project IDEAL-
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cited reference count 50
publisher ELSEVIER SCIENCE BV
publisher city AMSTERDAM
publisher address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
issn 0166-445X
29-character source abbreviation AQUAT TOXICOL
iso source abbreviation Aquat. Toxicol.
publication date JAN
year published 2016
volume 170
beginning page 371
ending page 376
digital object identifier (doi) 10.1016/j.aquatox.2015.10.018
page count 6
web of science category Marine & Freshwater Biology; Toxicology
subject category Marine & Freshwater Biology; Toxicology
document delivery number DB5OW
unique article identifier WOS:000368564500040
link http://www.sciencedirect.com/science/article/pii/S0166445X15300771