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title Toxicity of tributyltin (TBT) to the freshwater planarian Schmidtea mediterranea
authors Ofoegbu, PU; Simao, FCP; Cruz, A; Mendo, S; Soares, AMVM; Pestana, JLT
author full name Ofoegbu, Pearl U.; Simao, Fatima C. P.; Cruz, Andreia; Mendo, Snia; Soares, Amadeu M. V. M.; Pestana, Joao L. T.
title Toxicity of tributyltin (TBT) to the freshwater planarian Schmidtea mediterranea
nationality internacional
language English
document type Article
author keywords Tributyltin toxicity; Behavioural endpoints; Regeneration; Genotoxicity; Schmidtea mediterranea
abstract The freshwater planarian Schmidtea mediterranea, one of the best characterized animal models for regeneration research and developmental biology, is being recognised as a useful species for ecotoxicological studies. Sensitive endpoints related to planarians' behaviour and regeneration can be easily evaluated after exposure to environmental stressors. In this work the sensitivity of S. mediterranea to a gradient of environmentally relevant concentrations of TBT was studied using multiple endpoints like survival, locomotion, head regeneration and DNA damage. In addition, a feeding assay based on planarian's predatory behaviour was performed. Results indicated that TBT is toxic to planarians with LC50's of 1.87 mu g L-1 Sn and 1.31 mu g L-1 Sn at 48 h and 96 h of exposure respectively. Sub-lethal exposures to TBT significantly reduced locomotion and feeding, delayed head regeneration and caused DNA damage in planarians. The behavioural endpoints (feeding and locomotion) and head regeneration were the most sensitive parameters followed by DNA damage. Similar to other aquatic model organisms, S. mediterranea showed high sensitivity towards TBT exposure. Based on our results, and though further research is required concerning their sensitivity to other pollutants, the use of freshwater planarians as a model species in ecotoxicology is discussed. (C) 2016 Published by Elsevier Ltd.
author address [Ofoegbu, Pearl U.; Simao, Fatima C. P.; Cruz, Andreia; Mendo, Snia; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Ofoegbu, Pearl U.; Simao, Fatima C. P.; Cruz, Andreia; Mendo, Snia; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Ofoegbu, Pearl U.] Fed Univ Technol Owerri, Dept Biol, Owerri, Nigeria
reprint address Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address jpestana@ua.pt
funding agency and grant number Portuguese Science Foundation (FCT) [CESAM: UID/AMB/50017/2013]; Tertiary Education Trust Fund (TETFUND), Nigeria; FCT; POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) [SFRH/BPD/45342/2008, SFRH/BD/52567/2014]; project
funding text This work was supported by the Portuguese Science Foundation (FCT) through CESAM: UID/AMB/50017/2013. Pearl U. Ofoegbu acknowledges the Tertiary Education Trust Fund (TETFUND), Nigeria for doctoral grant. We are thankful to FCT and POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) for the post-doctoral fellowship of J.L.T. Pestana (SFRH/BPD/45342/2008) and Fatima C. P. Simao doctoral grant (SFRH/BD/52567/2014). Andreia Cruz was funded by the post-doctoral grant (BPD/UI88/2886/2013), from the project
cited references Pestana JLT, 2007, CHEMOSPHERE, V68, P1556, DOI 10.1016/j.chemosphere.2007.02.053; Raffa RB, 2001, J PHARMACOL TOXICOL, V45, P223, DOI 10.1016/S1056-8719(01)00152-6; Pra D, 2005, MUTAT RES-GEN TOX EN, V585, P79, DOI 10.1016/j.mrgentox.2005.04.002; Knakievicz T, 2008, CHEMOSPHERE, V71, P419, DOI 10.1016/j.chemosphere.2007.11.004; WEIS JS, 1987, ARCH ENVIRON CON TOX, V16, P321; Oehlmann J, 1996, SCI TOTAL ENVIRON, V188, P205, DOI 10.1016/0048-9697(96)05173-X; Oberdorster E, 1998, ARCH ENVIRON CON TOX, V34, P21; Hagger JA, 2005, MAR POLLUT BULL, V51, P811, DOI 10.1016/j.marpolbul.2005.06.044; Abbott A, 2000, SCI TOTAL ENVIRON, V258, P5, DOI 10.1016/S0048-9697(00)00505-2; Okoro HK, 2011, REV ENVIRON CONTAM T, V213, P27, DOI 10.1007/978-1-4419-9860-6_2; Wu HPP, 2011, J PHOTOCH PHOTOBIO B, V102, P156, DOI 10.1016/j.jphotobiol.2010.11.003; Nano GM, 2002, FITOTERAPIA, V73, P140, DOI 10.1016/S0367-326X(02)00014-X; Dowson PH, 1996, ESTUAR COAST SHELF S, V42, P551, DOI 10.1006/ecss.1996.0036; Hagger JA, 2002, AQUAT TOXICOL, V57, P243, DOI 10.1016/S0166-445X(01)00200-4; Talbot J, 2011, J EXP BIOL, V214, P1063, DOI 10.1242/jeb.052290; RUIZ JM, 1994, MAR ECOL PROG SER, V113, P105, DOI 10.3354/meps113105; Garcia O, 2004, MUTAT RES-FUND MOL M, V556, P25, DOI 10.1016/j.mrfmmm.2004.06.035; Stringer CE, 1917, P NATL ACAD SCI USA, V3, P691, DOI 10.1073/pnas.3.12.691; WALSH GE, 1986, ECOTOX ENVIRON SAFE, V12, P95, DOI 10.1016/0147-6513(86)90009-6; Yu A, 2013, AQUAT TOXICOL, V128, P158, DOI 10.1016/j.aquatox.2012.12.008; Mattioli M, 2003, MOL REPROD DEV, V65, P289, DOI 10.1002/mrd.10275; FENT K, 1992, ENVIRON POLLUT, V76, P187; Velma Venkatramreddy, 2009, Reviews on Environmental Health, V24, P129; Tang CH, 2009, J ENVIRON MONITOR, V11, P92, DOI 10.1039/b807864e; Nogueira PR, 2006, MAR POLLUT BULL, V52, P1611, DOI 10.1016/j.marpolbul.2006.05.017; SNOEIJ NJ, 1987, ENVIRON RES, V44, P335, DOI 10.1016/S0013-9351(87)80242-6; Schmidt K, 2006, J FRESHWATER ECOL, V21, P109, DOI 10.1080/02705060.2006.9664103; Mizuhashi S, 2000, NEUROSCI RES, V38, P35, DOI 10.1016/S0168-0102(00)00137-1; Diez S, 2002, WATER RES, V36, P905, DOI 10.1016/S0043-1354(01)00305-0; Diez S, 2009, J ENVIRON MANAGE, V90, pS25, DOI 10.1016/j.jenvman.2008.07.021; Ferraro MVM, 2004, GENET MOL BIOL, V27, P103, DOI 10.1590/S1415-47572004000100017; UNGER MA, 1988, ENVIRON TOXICOL CHEM, V7, P907, DOI 10.1897/1552-8618(1988)7[907:SBOTOE]2.0.CO;2; Ohji M, 2002, MAR ECOL PROG SER, V235, P171, DOI 10.3354/meps235171; Itow T, 1998, ARCH ENVIRON CON TOX, V35, P457; FENT K, 1992, ARCH ENVIRON CON TOX, V22, P428; Barroso CM, 2002, MAR POLLUT BULL, V44, P480, DOI 10.1016/S0025-326X(01)00260-0; Reddien PW, 2004, ANNU REV CELL DEV BI, V20, P725, DOI 10.1146/annurev.cellbio.20.010403.095114; Cheng Z, 2014, TOXICOL LETT, V225, P413, DOI 10.1016/j.toxlet.2014.01.010; Kovacevic G, 2009, WATER AIR SOIL POLL, V196, P333, DOI 10.1007/s11270-008-9781-1; Turner A, 2010, MAR POLLUT BULL, V60, P159, DOI 10.1016/j.marpolbul.2009.12.004; Barroso CM, 2000, MAR ECOL PROG SER, V201, P221, DOI 10.3354/meps201221; Fickova M, 2015, TOXICOL IN VITRO, V29, P727, DOI 10.1016/j.tiv.2015.02.007; Reddien PW, 2005, DEV CELL, V8, P635, DOI 10.1016/j.devcel.2005.02.014; Wang Y, 2012, B ENVIRON CONTAM TOX, V89, P704, DOI 10.1007/s00128-012-0760-2; Alonso A, 2011, CHEMOSPHERE, V84, P533, DOI 10.1016/j.chemosphere.2011.04.030; WEIS JS, 1987, ENVIRON HEALTH PERSP, V71, P77, DOI 10.2307/3430415; ORRENIUS S, 1992, ANN NEUROL, V32, pS33, DOI 10.1002/ana.410320708; ASTM, 2004, E119397 ASTM; Boelsterli U.A., 2003, MECHANISTIC TOXICOLO, P148; Inoue T., 2015, ZOOL LETT, V1, P1; International Maritime Organization (IMO), 2001, INT CONV CONTR HARMF; International Programme on Chemical Safety (IPCS), 1990, ENV HLTH CRIT, V116; Kent R., 1974, POLL EC FRESHW INV, P67; Knakievicz T., 2014, ECOTOXICOL ENV CONT, V9, P1; Oviedo N.J., 2008, COLD SPRING HARB PRO; Pagán Oné R, 2009, J Undergrad Neurosci Educ, V7, pA48; Rodrigues A.C.M., 2015, AQUAT TOXICOL, V170, P273; Alvarado AS, 2000, BIOESSAYS, V22, P578, DOI 10.1002/(SICI)1521-1878(200006)22:6<578::AID-BIES11>3.0.CO;2-#; Sánchez Alvarado A, 1998, Wound Repair Regen, V6, P413; Thain J.E., 1983, 1983EI3 ICES; Thorp J.H., 2001, ECOLOGY CLASSIFICATI
cited reference count 61
publisher city OXFORD
issn 0045-6535
29-character source abbreviation CHEMOSPHERE
iso source abbreviation Chemosphere
publication date APR
year published 2016
volume 148
beginning page 61
ending page 67
digital object identifier (doi) 10.1016/j.chemosphere.2015.12.131
page count 7
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number DF1KX
unique article identifier WOS:000371099700009