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title Toxicity of tributyltin (TBT) to the freshwater planarian Schmidtea mediterranea
authors Ofoegbu, PU; Simao, FCP; Cruz, A; Mendo, S; Soares, AMVM; Pestana, JLT
author full name Ofoegbu, Pearl U.; Simao, Fatima C. P.; Cruz, Andreia; Mendo, Snia; Soares, Amadeu M. V. M.; Pestana, Joao L. T.
title Toxicity of tributyltin (TBT) to the freshwater planarian Schmidtea mediterranea
nationality internacional
source CHEMOSPHERE
language English
document type Article
author keywords Tributyltin toxicity; Behavioural endpoints; Regeneration; Genotoxicity; Schmidtea mediterranea
keywords plus CAENORHABDITIS-ELEGANS; BIOLOGICAL-ACTIVITY; PHOXINUS-PHOXINUS; POLYCELIS-FELINA; DAPHNIA-MAGNA; COMET ASSAY; REGENERATION; BEHAVIOR; SEDIMENTS; EXPOSURE
abstract The freshwater planarian Schmidtea mediterranea, one of the best characterized animal models for regeneration research and developmental biology, is being recognised as a useful species for ecotoxicological studies. Sensitive endpoints related to planarians' behaviour and regeneration can be easily evaluated after exposure to environmental stressors. In this work the sensitivity of S. mediterranea to a gradient of environmentally relevant concentrations of TBT was studied using multiple endpoints like survival, locomotion, head regeneration and DNA damage. In addition, a feeding assay based on planarian's predatory behaviour was performed. Results indicated that TBT is toxic to planarians with LC50's of 1.87 mu g L-1 Sn and 1.31 mu g L-1 Sn at 48 h and 96 h of exposure respectively. Sub-lethal exposures to TBT significantly reduced locomotion and feeding, delayed head regeneration and caused DNA damage in planarians. The behavioural endpoints (feeding and locomotion) and head regeneration were the most sensitive parameters followed by DNA damage. Similar to other aquatic model organisms, S. mediterranea showed high sensitivity towards TBT exposure. Based on our results, and though further research is required concerning their sensitivity to other pollutants, the use of freshwater planarians as a model species in ecotoxicology is discussed. (C) 2016 Published by Elsevier Ltd.
author address [Ofoegbu, Pearl U.; Simao, Fatima C. P.; Cruz, Andreia; Mendo, Snia; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Ofoegbu, Pearl U.; Simao, Fatima C. P.; Cruz, Andreia; Mendo, Snia; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Ofoegbu, Pearl U.] Fed Univ Technol Owerri, Dept Biol, Owerri, Nigeria
reprint address Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address jpestana@ua.pt
funding agency and grant number Portuguese Science Foundation (FCT) [CESAM: UID/AMB/50017/2013]; Tertiary Education Trust Fund (TETFUND), Nigeria; FCT; POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) [SFRH/BPD/45342/2008, SFRH/BD/52567/2014]; project
funding text This work was supported by the Portuguese Science Foundation (FCT) through CESAM: UID/AMB/50017/2013. Pearl U. Ofoegbu acknowledges the Tertiary Education Trust Fund (TETFUND), Nigeria for doctoral grant. We are thankful to FCT and POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) for the post-doctoral fellowship of J.L.T. Pestana (SFRH/BPD/45342/2008) and Fatima C. P. Simao doctoral grant (SFRH/BD/52567/2014). Andreia Cruz was funded by the post-doctoral grant (BPD/UI88/2886/2013), from the project
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cited reference count 61
publisher PERGAMON-ELSEVIER SCIENCE LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
issn 0045-6535
29-character source abbreviation CHEMOSPHERE
iso source abbreviation Chemosphere
publication date APR
year published 2016
volume 148
beginning page 61
ending page 67
digital object identifier (doi) 10.1016/j.chemosphere.2015.12.131
page count 7
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number DF1KX
unique article identifier WOS:000371099700009