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title Multigenerational effects of carbendazim in Daphnia magna
authors Silva, ARR; Cardoso, DN; Cruz, A; Pestana, JLT; Mendo, S; Soares, AMVM; Loureiro, S
author full name Silva, Ana Rita R.; Cardoso, Diogo N.; Cruz, Andreia; Pestana, Joao L. T.; Mendo, Sonia; Soares, Amadeu M. V. M.; Loureiro, Susana
title Multigenerational effects of carbendazim in Daphnia magna
nationality internacional
source ENVIRONMENTAL TOXICOLOGY AND CHEMISTRY
language English
document type Article
author keywords Daphnia magna; Multigenerational effects; Toxicity; Carbendazim; Genotoxicity
keywords plus 3 SUCCESSIVE GENERATIONS; DNA-DAMAGE; CADMIUM RESISTANCE; COMET ASSAY; TOXICITY; RESPONSES; EXPOSURE; STRAUS; POPULATIONS; LONGISPINA
abstract Carbendazim is a fungicide largely used in agriculture as a plant protection product. As a result of agricultural runoffs, drainage, and leaching, it reaches surface waters at concentrations possibly hazardous to aquatic communities. Because of potential and continuous release of carbendazim to aquatic systems, long-term exposure to aquatic organisms should be addressed. To fill the knowledge gap, the present study evaluated the responses of multiple generations of Daphnia magna (clone K6) to an environmentally relevant concentration of carbendazim (5g/L). Twelve successive generations were evaluated, and the effects in these offspring were compared with those from a control population. Neonates' fitness was assessed through immobilization, reproduction, and feeding activity tests, along with the comet assay for in vivo DNA damage evaluation. Recovery from long-term exposure was also assessed. In the F5 generation, the results revealed that when daphnids were re-exposed to carbendazim, DNA damage was higher in daphnids continuously exposed to carbendazim than those from clean medium. After daphnids were moved to a clean medium, a low recovery potential was observed for DNA damage. Daphnids exposed continuously for 6 generations (F6) to carbendazim displayed an increase in feeding rates when re-exposed to carbendazim compared with F6 daphnids reared in clean medium. Continuous exposure of daphnids to carbendazim induced a significant increase in DNA damage from the F0 to the F12 generation. Deleterious effects of the multigenerational exposure to carbendazim were more prominent at a subcellular level (DNA damage) compared with the individual level. Environ Toxicol Chem 2017;36:383-394. (c) 2016 SETAC
author address [Silva, Ana Rita R.; Loureiro, Susana] Univ Aveiro, Dept Biol, Aveiro, Portugal; Univ Aveiro, Ctr Environm & Marine Studies CESAM, Aveiro, Portugal
reprint address Silva, ARR; Loureiro, S (reprint author), Univ Aveiro, Dept Biol, Aveiro, Portugal.
e-mail address ritas@ua.pt; sloureiro@ua.pt
researcherid number Loureiro, Susana/B-4462-2008;
orcid number Loureiro, Susana/0000-0002-5393-9623; Pestana, Joao/0000-0003-1322-2959
funding agency and grant number project RePulse-Responses of Daphnia magna Exposed to Chemical Pulses and Mixtures Throughout Generations [FCOMP-01-0124-FEDER-019321, FCT PTDC/AAC-AMB/117178/2010]; FEDER through COMPETE-Programa Operacional Factores de Competitividade; Portuguese Science Foundation (FCT) through CESAM [UID/AMB/50017/2013]; project MARPRO-Conservation of Marine Protected Species in Mainland Portugal through a doctoral fellowship [BD/UI88/5534/2011]; project Sustainable Use of Marine Resources-MARES [BPD/UI88/2886/2013]; QREN, Mais Centro-Programa Operacional Regional do Centro e Uniao Europeia/Fundo Europeu de Desenvolvimento Regional [CENTRO-07-ST24-FEDER-002033]; [PD/BD/52569/2014]; [SFRH/BPD/45342/2008]
funding text The present study was supported by project RePulse-Responses of Daphnia magna Exposed to Chemical Pulses and Mixtures Throughout Generations (FCOMP-01-0124-FEDER-019321; Refa. FCT PTDC/AAC-AMB/117178/2010); by funding from FEDER through COMPETE-Programa Operacional Factores de Competitividade; and by the Portuguese Science Foundation (FCT) through CESAM: UID/AMB/50017/2013. A.R.R. Silva was funded by project MARPRO-Conservation of Marine Protected Species in Mainland Portugal through a doctoral fellowship (BD/UI88/5534/2011); D. Cardoso was funded by doctoral grant PD/BD/52569/2014; A. Cruz was funded by an individual postdoctoral grant (BPD/UI88/2886/2013) within the project Sustainable Use of Marine Resources-MARES (CENTRO-07-ST24-FEDER-002033), financed by QREN, Mais Centro-Programa Operacional Regional do Centro e Uniao Europeia/Fundo Europeu de Desenvolvimento Regional; and J. Pestana was funded by an individual postdoctoral grant (SFRH/BPD/45342/2008).
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cited reference count 67
publisher WILEY
publisher city HOBOKEN
publisher address 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
issn 0730-7268
29-character source abbreviation ENVIRON TOXICOL CHEM
iso source abbreviation Environ. Toxicol. Chem.
publication date FEB
year published 2017
volume 36
issue 2
beginning page 383
ending page 394
digital object identifier (doi) 10.1002/etc.3541
page count 12
web of science category Environmental Sciences; Toxicology
subject category Environmental Sciences & Ecology; Toxicology
document delivery number EL5UL
unique article identifier WOS:000394686600012
link http://dx.doi.org/10.1002/etc.3541