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title Responses of the aquatic midge Chironomus riparius to DEET exposure
authors Campos, D; Gravato, C; Quintaneiro, C; Soares, AMVM; Pestana, JLT
author full name Campos, Diana; Gravato, Carlos; Quintaneiro, Carla; Soares, Amadeu M. V. M.; Pestana, Joao L. T.
title Responses of the aquatic midge Chironomus riparius to DEET exposure
nationality internacional
language English
document type Article
author keywords Personal care products; Freshwater insects; Emergent pollutants; Biochemical responses; N,N-diethyl-3-methylbenzamide
abstract N,N-diethyl-3-methylbenzamide (DEET) is the active ingredient of many commercial insect repellents. Despite being detected worldwide in effluents, surface water and groundwater, there is still limited information on DEET's toxicity toward non-target aquatic invertebrates. Thus, our main objective was to assess the effects of DEET in the life cycle of Chironomus riparius and assess its biochemical effects. Laboratory assays showed that DEET reduced developmental rates (reduced larval growth, delayed emergence) of C. riparius larvae and also caused a decrease in the size of adult midges. Concerning the biochemical responses, a short exposure to DEET caused no effects in lipid peroxidation, despite the significant inhibition of catalase and glutathione-S-transferase activities and of total glutathione contents. Moreover, inhibition of acetylcholinesterase activity was also observed showing neurotoxic effects. Environmental risk assessment of insect repellents is needed. Our results showed moderate toxicity of DEET toward C. riparius, however, due to their mode of action, indirect ecological effects of DEET and of other insect repellents cannot be excluded and should be evaluated. (C) 2015 Elsevier B.V. All rights reserved.
author address [Pestana, Joao L. T.] Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; Univ Aveiro, CESAM, Campus Univ Santiago, P-3810193 Aveiro, Portugal
reprint address Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address jpestana@ua.pt
researcherid number Soares, Amadeu/A-8304-2008; CESAM, UA/M-3762-2015; Gravato, Carlos Alexandre/D-4261-2013
orcid number Soares, Amadeu/0000-0003-0879-9470; Gravato, Carlos Alexandre/0000-0003-0562-6945
funding agency and grant number Portuguese Foundation for Science and Technology (FCT) [CESAM: UID/AMB/50017/2013]; FCT [SFRH/BD/87370/2012, SFRH/BPD/45342/2008, SFRH/BPD/89951/2012]; POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) [IF/1401/2014]
funding text This work was supported by the Portuguese Foundation for Science and Technology (FCT) through CESAM: UID/AMB/50017/2013. FCT also financed a doctoral grant to Diana Campos (SFRH/BD/87370/2012), a post-doctoral research grant to Joao L.T. Pestana (SFRH/BPD/45342/2008) and Carla Quintaneiro (SFRH/BPD/89951/2012). We also thank FCT and POPH/FSE (Programa Operacional Potencial Humano/Fundo Social Europeu) for the contract of C. Gravato (IF/1401/2014).
cited references Abu-Qare A, 2000, TOXICOL LETT, V117, P151, DOI 10.1016/S0378-4274(00)00257-5; Abu-Qare AW, 2001, TOXICOL LETT, V121, P127, DOI 10.1016/S0378-4274(01)00330-7; Aronson D, 2012, INTEGR ENVIRON ASSES, V8, P135, DOI 10.1002/ieam.271; BAKER MA, 1990, ANAL BIOCHEM, V190, P360, DOI 10.1016/0003-2697(90)90208-Q; BIRD RP, 1984, METHOD ENZYMOL, V105, P299; Bohbot JD, 2012, NEUROPHARMACOLOGY, V62, P2086, DOI 10.1016/j.neuropharm.2012.01.004; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1006/abio.1976.9999; Brausch JM, 2011, CHEMOSPHERE, V82, P1518, DOI 10.1016/j.chemosphere.2010.11.018; Calza P, 2011, SCI TOTAL ENVIRON, V409, P3894, DOI 10.1016/j.scitotenv.2011.06.006; Campos D, 2014, ECOTOXICOLOGY, V23, P830, DOI 10.1007/s10646-014-1223-9; Claiborne A., 1985, HDB METHODS OXYGEN R, P283; Corbel V, 2009, BMC BIOL, V7, DOI 10.1186/1741-7007-7-47; Costanzo SD, 2007, SCI TOTAL ENVIRON, V384, P214, DOI 10.1016/j.scitotenv.2007.05.036; Crane M, 2002, ECOTOX ENVIRON SAFE, V53, P361, DOI 10.1016/S0147-6513(02)00038-6; Crespo JG, 2011, J INSECT SCI, V11, DOI 10.1673/031.011.6201; Ditzen M, 2008, SCIENCE, V319, P1838, DOI 10.1126/science.1153121; Dogan EB, 1999, MED VET ENTOMOL, V13, P97, DOI 10.1046/j.1365-2915.1999.00145.x; ELLMAN GL, 1961, BIOCHEM PHARMACOL, V7, P88, DOI 10.1016/0006-2952(61)90145-9; Glassmeyer ST, 2005, ENVIRON SCI TECHNOL, V39, P5157, DOI 10.1021/es048120k; Guilhermino L, 1996, B ENVIRON CONTAM TOX, V57, P979; HABIG WH, 1974, J BIOL CHEM, V249, P7130; Hellestad VJ, 2011, CELL BIOL TOXICOL, V27, P149, DOI 10.1007/s10565-010-9177-z; Klaschka U, 2008, ENVIRON SCI POLLUT R, V15, P452, DOI 10.1007/s11356-008-0019-y; Leal WS, 2014, CURR OPIN INSECT SCI, V6, P93, DOI 10.1016/j.cois.2014.10.007; Lee Y, 2010, NEURON, V67, P555, DOI 10.1016/j.neuron.2010.07.006; Lilley TM, 2012, ENVIRON SCI TECHNOL, V46, P7382, DOI 10.1021/es300536t; Liu F, 2013, J INSECT PHYSIOL, V59, P1169, DOI 10.1016/j.jinsphys.2013.08.016; MICHAEL AG, 1974, MOSQ NEWS, V34, P32; OECD, 2004, EFF BIOT SYST TEST 2; OHKAWA H, 1979, ANAL BIOCHEM, V95, P351, DOI 10.1016/0003-2697(79)90738-3; Pedrouzo M, 2011, TRAC-TREND ANAL CHEM, V30, P749, DOI 10.1016/j.trac.2011.01.009; Pellegrino M, 2011, NATURE, V478, P511, DOI 10.1038/nature10438; Pery ARR, 2002, ENVIRON TOXICOL CHEM, V21, P2507, DOI 10.1897/1551-5028(2002)021<2507:AMATLF>2.0.CO;2; Pestana JLT, 2009, AQUAT TOXICOL, V93, P138, DOI 10.1016/j.aquatox.2009.04.008; Ponlawat A, 2007, J MED ENTOMOL, V44, P422, DOI 10.1603/0022-2585(2007)44[422:AABSIM]2.0.CO;2; Prajapati V, 2005, BIORESOURCE TECHNOL, V96, P1749, DOI 10.1016/j.biortech.2005.01.007; Rodrigues ACM, 2015, SCI TOTAL ENVIRON, V508, P506, DOI 10.1016/j.scitotenv.2014.12.021; Rodrigues ACM, 2015, ENVIRON POLLUT, V207, P273, DOI 10.1016/j.envpol.2015.09.035; Schoenig GP, 1999, TOXICOL SCI, V47, P99, DOI 10.1093/toxsci/47.1.99; Seo J, 2005, ARCH ENVIRON CON TOX, V48, P323, DOI 10.1007/s00244-004-0029-9; Slaninova A., 2014, BIOMED RES INT, V2014, P8; Stanczyk NM, 2010, P NATL ACAD SCI USA, V107, P8575, DOI 10.1073/pnas.1001313107; Stuart M, 2012, SCI TOTAL ENVIRON, V416, P1, DOI 10.1016/j.scitotenv.2011.11.072; Syed Z, 2008, P NATL ACAD SCI USA, V105, P13598, DOI 10.1073/pnas.0805312105; USEPA (U.S. Environmental Protection Agency), 1998, EPA738R98010 DEET OF; Weeks JA, 2012, INTEGR ENVIRON ASSES, V8, P120, DOI 10.1002/ieam.1246; Weigel S, 2004, CHEMOSPHERE, V56, P583, DOI 10.1016/j.chemoosphere.2004.05.015; Xia YF, 2008, P NATL ACAD SCI USA, V105, P6433, DOI 10.1073/pnas.0801007105; Xu PX, 2014, P NATL ACAD SCI USA, V111, P16592, DOI 10.1073/pnas.1417244111; Yi X, 2014, J INSECT PHYSIOL, V65, P51, DOI 10.1016/j.jinsphys.2014.05.007; Yoon Y, 2010, SCI TOTAL ENVIRON, V408, P636, DOI 10.1016/j.scitotenv.2009.10.049
cited reference count 51
publisher city AMSTERDAM
publisher address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
issn 0166-445X
29-character source abbreviation AQUAT TOXICOL
iso source abbreviation Aquat. Toxicol.
publication date MAR
year published 2016
volume 172
beginning page 80
ending page 85
digital object identifier (doi) 10.1016/j.aquatox.2015.12.020
page count 6
web of science category Marine & Freshwater Biology; Toxicology
subject category Marine & Freshwater Biology; Toxicology
document delivery number DD7GS
unique article identifier WOS:000370092300009