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  Helena Cristina Correia de Oliveira  
  Phone: +351 234 370 777 / Ext.22717  
  e-mail: holiveira@ua.pt  
  Category: Post-Doctoral fellow  
  Department: Department of Biology  
  Research group: Ecotoxicology
 
ISI Web of KnowledgeSM search factor: ResearcherID: M-5068-2013  
  Direct URL for this page: http://www.cesam.ua.pt/holiveira  
     
  Title: Evaluation of the pulmonary toxicity potential of graphene nanomaterials: cytotoxicity, genotoxicity and metabolic profiling
  Supervisor: Mário Guilherme Garcês Pacheco
  Category: BO
  Grant type: BPD
  Funding entity: FCT
  Grant reference: SFRH/BPD/111736/2015
  Start date: 2016-03-01
  End date: 2019-02-28
Current activity

Post-doctoral researcher financed by FCT (grant reference: SFRH/BPD/111736/2015). 

Academic qualifications

 2009- PhD in Biology, University of Aveiro, Portugal


2002- M.Sc. in Coastal Zones Sciences, University of Aveiro, Portugal


1998- Degree in Biology, University of Aveiro, Portugal

Research interests

Toxicity of metals and metal nanoparticles in in vitro models


Evaluation of the effects of phytocompounds on tumoral cells


Application of flow cytometry to assess functional parameters in plant and animal cells

Theses

H. Oliveira (2009) Avaliação do efeito de metais pesados na fertilidade do ratinho. PhD thesis.


H. Oliveira (2002) Estudo bioquímico de videiras (Vitis vinifera L.) cultivadas in vitro em ambiente salino e infectadas com Phaeomoniella chlamydospora. M.Sc. thesis

COST Actions

Member of the management committee:


CM1403: The European upconversion network - from the design of photon-upconverting nanomaterials to biomedical applications- as Management Committee Member. Leader of the Working Group 5-Toxicology http://www.ucnp.eu/


CA15107: Multi-Functional Nano-Carbon Composite Materials Network (MultiComp). http://www.cost.eu/COST_Action.....


TD1204: Modelling Nanomaterial Toxicity (MODENA) http://www.cost.eu/COST_Action.....

Participation in research projects

"PLATOX:In vitro and in vivo investigations to generate validated toxicity data of graphene nanoplatelets vs. a carbon black reference" (2015-2017). ERA­SIINN/0003/2013


"Metabolic profiling: a novel tool for evaluation of the toxicological and biological effects of nanomaterials" (2012-2015). PTDC/SAU-TOX/120953/2010


"BIO.REM: Integrating multiple toxicological BIOmarkers in a phytoREMemediation assay of Pb and Cd contaminated sites" (2011-2014). PTDC/AAC-MB/112804/2009


"ASSAY - Assessment of fate and effects of Silver nanoparticleS in Aquatic ecosystems" (2011-2014)PTDC/AAC-AMB/113649/2009


  "Técnicas de diagnóstico tradicionais e inovadoras na biomonitorização da fertilidadade masculina". UA- Projecto I&DT, Ciências e Tecnologias da Saúde/22. Concluded in 2005


 "Estudo bioquímico e ultraestrutural de videiras com o síndroma da esca e caracterização de fungos responsáveis pela doença". MCT-PraxisXXI-POCTI/AGR/C/11142. Concluded in 2002

Supervision/co-supervision of post-graduation students

PhD:


Joana Carolina Quintela Carrola. Thesis: “Evaluation of the biological effects and toxicity mechanisms of silver nanoparticles through metabolic profiling of human cells”. PhD in Nanosciences and nanotechnology. University of Aveiro.Concluded in 2017


Verónica Bastos. Thesis: “Do metal-based nanomaterials induce cytotoxicity and genotoxicity in human intestinal cells?”. PhD in Biology. University of Aveiro. Concluded in 2016.


Maria Cristina Monteiro. Thesis: "Cytogenicity of chromium and cadmium in plants and in human cells". PhD in Biology. University of Aveiro. Concluded in 2016


MASTER:


Débora Reis “Characterization of the cytotoxic potential of graphene nanoplatelets in lung cells” MsC in Molecular and Cellular Biology. Department of Biology, University of Aveiro. Concluded in 2016


Juliane Sousa. “Cytotoxic Potential of Graphene Oxide in Human Lung Adenocarcinoma Cell Line” MsC in Molecular and Cellular Biology. Department of Biology, University of Aveiro. Concluded in 2016


Maryam Nasirpour. Thesis: “Silver nanoparticles synthesized using plant extracts: toxicity screening and metabolic profiling in human cells”. FAME Master Student. University of Aveiro. Concluded in 2015.


Ana Catarina Menezes. Thesis: “Prevention of Photocarcinogenesis by Agonists of 5-HT1A and Antagonists of 5-HT2A Receptors” MsC in Biopharmaceutical Sciences, University of Lisboa. Concluded in 2015.


Sandra Cristina Pinto Martins. Thesis “Assessment of circulating endothelial progenitor cells by flow cytometry” MsC in Molecular and Cellular Biology. Departament of Biology, Universidade de Aveiro. Concluded in 2015.


Corine Santos Reis. Thesis: “Genotoxic effects of silver nanoparticles on lung cells” MsC in Applied Biology. Department of Biology, University of Aveiro. Concluded in 2013.


Fernanda Rosário. Thesis: “Effects of Silver Nanoparticles on osteosarcoma and lung cell lines”. MsC in Applied Biology. Department of Biology, University of Aveiro. Concluded  in 2012.


Catarina Remédios. Thesis: “Cytotoxic effect of sulforaphane in Human osteosarcoma cells”. MsC in Applied Biology. Department of Biology, University of Aveiro. Concluded  in 2012.


Maria Eugénia Costa. Thesis: “Oxidative Stress Induction by Sulforaphane on human Osteosarcoma”. MsC in Applied Biology. Department of Biology, University of Aveiro.  Concluded  in 2012.


Marta Isabel Esteves Pinheiro. Thesis: “Alterações no metaboloma de queratinócitos por exposição ao carragenano como veículo de entrega de fármacos”. MsC in Biochemistry. Department of Chemistry, University of Aveiro. Concluded in 2012


Juliana Sousa. Análise de osteoblastos in vitro por microscopia.  MsC in Molecular and Cellular Biology. Department of Biology, University of Aveiro. Concluded  in 2012.


Verónica Bastos. Thesis:" Genotoxic effects of environmental contaminants in dogs." MSc in Biologia Aplicada ramo de Toxicologia e Ecotoxicologia Departamento de Biologia, Universidade de Aveiro. Concluded in 2011.


Pedro Amaral. Thesis:" Evaluation of DNA damage induced by sulforaphane on MG-63 cells". MSc in Biologia Aplicada - Ramo de Biologia Molecular, Departamento de Biologia, Universidade de Aveiro. Concluded in 2011.


Tiago Pinto. Thesis:"Studies on cadmium induced toxicity in human osteoblasts: apoptosis and oxidative stress". MSc in Biologia Aplicada - Ramo de Biologia Molecular, Departamento de Biologia, Universidade de Aveiro. Concluded in 2011.


Francisco Pinho. Thesis:" Evaluation of the cytotoxic effects of cadmium in human osteobolasts". MSc in Biologia Aplicada - Ramo de Biologia Molecular, Departamento de Biologia, Universidade de Aveiro. Concluded in 2011.

Peer review of international scientific journals

  • Reproductive Toxicology

  • Journal of the Serbian Chemical Society

  • Environmental and Experimental Botany

  • Human and Experimental Toxicology

  • Australasian Journal of Plant Pathology

  • Urology Journal

  • Ecotoxicology and Environmental Safety

  • British Journal of Pharmaceutical Research

  • Plant Physiology and Biochemistry

  • Turkish Journal of Biology

  • Environmental Chemistry Letters

  • British Journal of Medicine and Medical Research

  • Life Sciences

  • Particle and Fibre Toxicology

  • Nanotoxicology

  • Toxicology in Vitro

  • Beilstein Journal of Nanotechnology

  • Ecological Indicators

  • Drug and Chemical Toxicology

Highlight

Invited to co-editor of the special issue “Legacy and emerging contaminants in plants: From the Gene to the Field” to the Journal of Botany

Scientific Supervision
PhD (Co-supervisor): Verónica Isabel Correia Bastos
(ex-CESAM member)
PhD (Co-supervisor): Joana Carolina Quintela Carrola
(not a CESAM member)
PhD (Co-supervisor): Maria Cristina Monteiro
(ex-CESAM member)

Master (Main Supervisor): Maryam Nasirpour
(not a CESAM member)
Master (Main Supervisor): Débora Reis
(not a CESAM member)
Master (Main Supervisor): Juliane Viegas
(not a CESAM member)
Master (Main Supervisor): Bruna Teixeira
(not a CESAM member)
Master (Main Supervisor): Carolina Roxo
(not a CESAM member)
Master (Main Supervisor): Sandra Cristina Pinto Martins
(not a CESAM member)
Master (Main Supervisor): Francisco Carvalho Vieira Pinho
(not a CESAM member)
Master (Main Supervisor): Tiago José de Assunção Carvalho e Pedrosa Pinto
(not a CESAM member)
Master (Main Supervisor): Pedro Miguel da Costa Amaral Pinto
(not a CESAM member)
Master (Main Supervisor): Fernanda Oliveira Esteves Rosário
Master (Main Supervisor): Catarina Fortuna dos Remédios
(ex-CESAM member)
Master (Main Supervisor): Maria Eugénia Marques da Costa
(ex-CESAM member)
Master (Co-supervisor): Ana Catarina Menezes
(not a CESAM member)
Master (Co-supervisor): Ana Catarina Menezes
(not a CESAM member)
Master (Co-supervisor): Corine Reis
(not a CESAM member)
Master (Co-supervisor): Verónica Isabel Correia Bastos
(not a CESAM member)
Master (Co-supervisor): Juliana Sousa
(not a CESAM member)
Master (Co-supervisor): Marta Isabel Esteves Pinheiro
(not a CESAM member)
Master (Co-supervisor): Juliana Moreira Crespo
(not a CESAM member)

bolseiro investigação (Main Supervisor): Catarina Menezes
(not a CESAM member)
Pesquisa (Main Supervisor): Fábio Campos
(not a CESAM member)
Pesquisa (Main Supervisor): Ruben Afonso
(not a CESAM member)
Pesquisa (Main Supervisor): Carolina Mota
(not a CESAM member)
Pesquisa (Main Supervisor): Ânia Micaelo
(not a CESAM member)
Research fellow (Master) (Main Supervisor): Ana Isabel da Silva Mendes
(not a CESAM member)
Research fellow (Master) (Main Supervisor): Ana Catarina Menezes
(not a CESAM member)
Pesquisa (Main Supervisor): Diana Roberta da Cruz Ribeiro
(not a CESAM member)
Pesquisa (Main Supervisor): Catarina Remédios
(not a CESAM member)
Projeto (Main Supervisor): Mariana Mendes Pinto
(not a CESAM member)
Short term scientific mission (Main Supervisor): Cynthia Kembuan
(not a CESAM member)
Short term scientific mission (Main Supervisor): Anna Ekner-Grzyb
(not a CESAM member)
Projeto (Co-supervisor): Daniel Carvalho
(not a CESAM member)



Projects
HOTsPOT - Novel upconversion nanoparticles for multimodal therapy of melanoma: pHOtothermal Therapy, PhOtodynamic therapy and chemoTherapy [see details]

Chemical and toxicological SOurce PROfiling of particulate matter in urban air - SOPRO [see details]

GOGOFROG: Alternative methods to animal experimentation for amphibian risk assessment: cell lines and spermatozoa [see details]

NanoReproTox-Unraveling the ecological impacts of nanoparticles toxicity in the reproduction of marine organisms [see details]

Nanoheaters and nanothermometers playing together: towards applications in Brownian motion and hyperthermia [see details]

PLATOX-In vitro and in vivo investigations to generate validated toxicity data of graphene nanoplatelets vs. a carbon black reference [see details]

Metabolic profiling: a novel tool for evaluation of the toxicological and biological effects of nanomaterials [see details]

ASSAY: Assessment of fate and effects of Silver nanoparticleS in Aquatic ecosYstems [see details]

BioREM [see details]

Publications
  1. Rosário F, Hoet P, Nogueira AJA, Santos C, and Oliveira H (In press) Differential pulmonary in vitro toxicity of two small sized polyvinylpyrrolidone coated silver nanoparticles. Journal Of Toxicology And Environmental Health, Part A. (see details) ISI paper
  2. Monteiro C, Ferreira de Oliveira JMP, Pinho F, Bastos V, OliveiraH, Peixoto F, Santos C (In press) Biochemical and transcriptional analyses of cadmium-induced mitochondrial dysfunction and oxidative stress in human osteoblasts. Journal Of Toxicology And Environmental Health, Part A. (see details) ISI paper
  3. Menezes A.C., Carvalheiro M., de Oliveira J.M.P.F., Ascenso A., Oliveira H. (2018) Cytotoxic effect of the serotonergic drug 1-(1-Naphthyl)piperazine against melanoma cells. Toxicology In Vitro. 47, 72-78. (see details) ISI paper
  4. de Oliveira J.M.P.F., Pacheco A.R., Coutinho L., Oliveira H., Pinho S., Almeida L., Fernandes E., Santos C. (2018) Combination of etoposide and fisetin results in anti-cancer efficiency against osteosarcoma cell models. Archives Of Toxicology. 92, 3, 1205-1214. (see details) ISI paper
  5. Dias M.C., Pinto D.C.G.A., Correia C., Moutinho-Pereira J., Oliveira H., Freitas H., Silva A.M.S., Santos C. (2018) UV-B radiation modulates physiology and lipophilic metabolite profile in Olea europaea. Journal Of Plant Physiology. 222, 39-50. (see details) ISI paper
  6. Carrola J., Pinto R.J.B., Nasirpour M., Freire C.S.R., Gil A.M., Santos C., Oliveira H., Duarte I.F. (2018) NMR Metabolomics Reveals Metabolism-Mediated Protective Effects in Liver (HepG2) Cells Exposed to Subtoxic Levels of Silver Nanoparticles. Journal Of Proteome Research. 17, 4, 1636-1646. (see details) ISI paper
  7. C.I.M. Santos, G. Gonçalves, M. Cicuéndez, I. Mariz, V.S. Silva, H Oliveira, F. Campos, S. I. Vieira, P.A.A.P. Marques, E.M.S. Maçôas, M.G.P.M.S. Neves, J.M.G. Martinho (2018) Biocompatible hybrids based on nanographene oxide covalently linked to glycolporphyrins: Synthesis, characterization and biological evaluation. Carbon. 135, 202-214. (see details) ISI paper
  8. Cicuéndez M, Flores J, Oliveira H, Portolés MT, Vallet-Regi M, Vila M, Duarte IF (2018) Metabolomic response of osteosarcoma cells to nanographene oxide-mediated hyperthermia. Materials Science And Engineering: C. 91, 340-348. (see details) ISI paper
  9. Bastos V., Ferreira-de-Oliveira J.M.P., Carrola J., Daniel-da-Silva A.L., Duarte I.F., Santos C., Oliveira H. (2017) Coating independent cytotoxicity of citrate- and PEG-coated silver nanoparticles on a human hepatoma cell line. Journal Of Environmental Sciences. 51, 191-201. (see details) ISI paper
  10. Bastos V., Duarte I.F., Santos C., Oliveira H. (2017) Genotoxicity of citrate-coated silver nanoparticles to human keratinocytes assessed by the comet assay and cytokinesis blocked micronucleus assay. Environmental Science And Pollution Research. 24, 5, 5039-5048. (see details) ISI paper
  11. Ramos P.A.B., Guerra A.R., Guerreiro O., Santos S.A.O., Oliveira H., Freire C.S.R., Silvestre A.J.D., Duarte M.F. (2017) Antiproliferative Effects of Cynara cardunculus L. var. altilis (DC) Lipophilic Extracts. International Journal Of Molecular Sciences. 18, 1, (see details) ISI paper
  12. Coutinho L., Oliveira H., Pacheco A.R., Almeida L., Pimentel F., Santos C., de Oliveira J.M.P.F. (2017) Hesperetin-etoposide combinations induce cytotoxicity in U2OS cells: Implications on therapeutic developments for osteosarcoma. Dna Repair. 50, 36-42. (see details) ISI paper
  13. Silva S., Silva P., Oliveira H., Gaivao I., Matos M., Pinto-Carnide O., Santos C. (2017) Pb low doses induced genotoxicity in Lactuca sativa plants. Plant Physiology And Biochemistry. 112, 109-116. (see details) ISI paper
    Link: https://authors.elsevier.com/a/1UKm33VH9WmMyc
  14. Lamego I., Marques M.P.M., Duarte I.F., Martins A.S., Oliveira H., Gil A.M. (2017) Impact of the Pd(2)Spermine Chelate on Osteosarcoma Metabolism: An NMR Metabolomics Study. Journal Of Proteome Research. 16, 4, 1773-1783. (see details) ISI paper
  15. Bastos V., Duarte I.F., Santos C., Oliveira H. (2017) A study of the effects of citrate-coated silver nanoparticles on RAW 264.7 cells using a toolbox of cytotoxic endpoints. Journal Of Nanoparticle Research. 19, 5, (see details) ISI paper
  16. Saidi L., Vilela C., Oliveira H., Silvestre A.J.D., Freire C.S.R. (2017) Poly(N-methacryloyl glycine)/nanocellulose composites as pH-sensitive systems for controlled release of diclofenac. Carbohydrate Polymers. 169, 357-365. (see details) ISI paper
  17. Silva S., Craveiro S.C., Oliveira H., Calado A.J., Pinto R.J.B., Silva A.M.S., Santos C. (2017) Wheat chronic exposure to TiO2-nanoparticles: Cyto- and genotoxic approach. Plant Physiology And Biochemistry. 121, 89-98. (see details) ISI paper
  18. Silva S., Oliveira H., Silva A.M.S., Santos C. (2017) The cytotoxic targets of anatase or rutile plus anatase nanoparticles depend on the plant species. Biologia Plantarum. 61, 4, 717-725. (see details) ISI paper
  19. Menezes A.C., Raposo S., Simoes S., Ribeiro H., Oliveira H., Ascenso A. (2016) Prevention of Photocarcinogenesis by Agonists of 5-HT1A and Antagonists of 5-HT2A Receptors. Molecular Neurobiology. 53, 2, 1145-1164. (see details) ISI paper
    Link: http://link.springer.com/article/10.1007%2Fs12035-014-9068-z
  20. Carrola J., Bastos V., de Oliveira J.M.P.F., Oliveira H., Santos C., Gil A.M., Duarte I.F. (2016) Insights into the impact of silver nanoparticles on human keratinocytes metabolism through NMR metabolomics. Archives Of Biochemistry And Biophysics. 589, 53-61. (see details) ISI paper
    Link: http://www.sciencedirect.com/science/article/pii/S0003986115300485
  21. Ascenso A., Pedrosa T., Pinho S., Pinho F., de Oliveira J.M.P.F., Marques H.C., Oliveira H., Simoes S., Santos C. (2016) The Effect of Lycopene Preexposure on UV-B-Irradiated Human Keratinocytes. Oxidative Medicine And Cellular Longevity. (see details) ISI paper
    Link: file:///C:/Users/Helena/Downloads/903961.pdf
  22. Silva S., Oliveira H., Craveiro S.C., Calado A.J., Santos C. (2016) Pure anatase and rutile plus anatase nanoparticles differently affect wheat seedlings. Chemosphere. 151, 68-75. (see details) ISI paper
  23. Debasu M.L., Brites C.D.S., Balabhadra S., Oliveira H., Rocha J., Carlos L.D. (2016) Nanoplatforms for Plasmon-Induced Heating and Thermometry. Chemnanomat. 2, 6, 520-527. (see details) ISI paper
  24. Bastos V., de Oliveira J.M.P.F., Brown D., Jonhston H., Malheiro E., Daniel-da-Silva A.L., Duarte I.F., Santos C., Oliveira H. (2016) The influence of Citrate or PEG coating on silver nanoparticle toxicity to a human keratinocyte cell line. Toxicology Letters. 249, 29-41. (see details) ISI paper
    Link: http://ac.els-cdn.com/S0378427416300418/1-s2.0-S0378427416300418-main.pd...
  25. Carrola J., Bastos V., Jarak I., Oliveira-Silva R., Malheiro E., Daniel-da-Silva A.L., Oliveira H., Santos C., Gil A.M., Duarte I.F. (2016) Metabolomics of silver nanoparticles toxicity in HaCaT cells: structure-activity relationships and role of ionic silver and oxidative stress. Nanotoxicology. 10, 8, 1105-1117. (see details) ISI paper
  26. Nunes S., Santos C., Moutinho-Pereira J., Correia C., Oliveira H., de Oliveira J.M.F., Pereira V.T., Almeida T., Marum L., Dias M.C. (2016) Physiological characterization and true-to-typeness evaluation of in vitro and ex vitro seedlings of Pinus elliottii: A contribution to breeding programs. Plant Physiology And Biochemistry. 107, 222-227. (see details) ISI paper
  27. Bastos V., Brown D., Johnston H., Daniel-da-Silva A.L., Duarte I.F., Santos C., Oliveira H. (2016) Inflammatory responses of a human keratinocyte cell line to 10 nm citrate- and PEG-coated silver nanoparticles. Journal Of Nanoparticle Research. 18, 7, (see details) ISI paper
    Link: 10.1007/s11051-016-3515-x
  28. Rosario F., Hoet P., Santos C., Oliveira H. (2016) Death and cell cycle progression are differently conditioned by the AgNP size in osteoblast-like cells. Toxicology. 368, 103-115. (see details) ISI paper
  29. Josee M.D.S., Corredoira E., Oliveira H., Santos C. (2015) Cryopreservation of somatic embryos of Alnus glutinosa (L.) Gaertn. and confirmation of ploidy stability by flow cytometry. Plant Cell Tissue And Organ Culture. 123, 3, 489-499. (see details) ISI paper
    Link: http://link.springer.com/article/10.1007%2Fs12035-014-9068-z
  30. Ferreira de Oliveira JMP, Costa M, Pedrosa T, Pinto P, Remedios C, Oliveira H, Pimentel F, Almeida L, Santos C (2014) Sulforaphane Induces Oxidative Stress and Death by p53-Independent Mechanism: Implication of Impaired Glutathione Recycling. Plos One. 9, 3, e92980 (see details) ISI paper
    Link: http://dx.doi.org/10.1371/journal.pone.0092980
  31. Dias M.C., Oliveira H., Costa A., Santos C. (2014) Improving elms performance under drought stress: The pretreatment with abscisic acid. Environmental And Experimental Botany. 100, 64-73. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.envexpbot.2013.12.013
  32. Dias M.C., Correia C., Moutinho-Pereira J., Oliveira H., Santos C. (2014) Study of the effects of foliar application of ABA during acclimatization. Plant Cell Tissue And Organ Culture. 117, 2, 213-224. 1573-5044. (see details) ISI paper
    Link: http://dx.doi.org/10.1007/s11240-014-0434-3
  33. Ferreira de Oliveira J.M.P., Remédios C., Oliveira H., Pinto P., Pinho F., Pinho S., Costa M., Santos C. (2014) Sulforaphane Induces DNA Damage and Mitotic Abnormalities in Human Osteosarcoma MG-63 Cells: Correlation with Cell Cycle and Apoptosis. Nutrition And Cancer-an International Journal. 66, 2, 325-334. (see details) ISI paper
    Link: http://www.tandfonline.com/doi/full/10.1080/01635581.2014.864777#.UvT4K5...
  34. Ascenso A., Ribeiro H., Marques H.C., Oliveira H., Santos C., Simoes S. (2014) Is Tretinoin Still a Key Agent for Photoaging Management?. Mini-reviews In Medicinal Chemistry. 14, 8, 629-641. (see details) ISI paper
  35. Ascenso A., Ribeiro H., Marques H.C., Oliveira H., Santos C., Simoes S. (2014) Chemoprevention of photocarcinogenesis by lycopene. Experimental Dermatology. 23, 12, 874-878. (see details) ISI paper
    Link: http://onlinelibrary.wiley.com/doi/10.1111/exd.12491/abstract
  36. Ascenso A., Salgado A., Euleterio C., Praca F.G., Bentley M.V.L.B., Marques H.C., Oliveira H., Santos C., Simoes S. (2014) In vitro and in vivo topical delivery studies of tretinoin-loaded ultradeformable vesicles. European Journal Of Pharmaceutics And Biopharmaceutics. 88, 1, 48-55. (see details) ISI paper
  37. Oliveira H., Monteiro C., Pinho F., Pinho S., de Oliveira J.M.P.F., Santos C. (2014) Cadmium-induced genotoxicity in human osteoblast-like cells. Mutation Research-genetic Toxicology And Environmental Mutagenesis. 775, 38-47. (see details) ISI paper
    Link: http://www.sciencedirect.com/science/article/pii/S138357181400271X
  38. de Oliveira J.M.P.F., Oliveira H., Pinho S., Pimentel F., Almeida L., Van Zoelen E.J.J., Santos C. (2014) Cytotoxic and genotoxic activity of fisetin (3, 3 ', 4 ', 7-tetrahydroxyflavone) in an osteosarcoma in vitro model. Planta Medica. 80, 16, 1412-1412. (see details) ISI paper
  39. Oliveira H., Costa A., Santos C. (2013) NaCl and Phaeomoniella chlamydospora affect differently starch and sucrose metabolism in grapevines. Acta Scientiarum-agronomy. 35, 2, 153-159. (see details) ISI paper
    Link: http://dx.doi.org/10.1021/jf401368w
  40. Nora S., Castro S., Loureiro J., Gonçalves A.C., Oliveira H., Castro M., Santos C. , Silveira P. (2013) Flow cytometric and karyological analyses of Calendula species from Iberian Peninsula. Plant Systematics And Evolution. 299, 5, 853-864. (see details) ISI paper
    Link: http://dx.doi.org/10.1007/s00606-013-0767-0
  41. Ascenso A., Pinho S., Eleutério C., Praça F., Oliveira H., Bentley M.V., Santos C., Silva O., Simões S. (2013) Lycopene from Tomatoes:Vesicular Nanocarrier Formulations for Dermal Delivery. Journal Of Agricultural And Food Chemistry. 61, 30, 7284-7293. (see details) ISI paper
    Link: http://dx.doi.org/10.1021/jf401368w
  42. Matos R.C., Bessa M., Oliveira H., Gonçalves F., Pereira M.L., Nunes B. (2013) Mechanisms of kidney toxicity for chromium- and arsenic-based preservatives: Potential involvement of a pro-oxidative pathway. Environmental Toxicology And Pharmacology. 36, 3, 929-936. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.etap.2013.08.006
  43. Ferreira M., Matos R.C., Oliveira H., Nunes B., Pereira M.L. (2012) Impairment of mice spermatogenesis by sodium arsenite. Human & Experimental Toxicology. 31, 3, 290-302. (see details) ISI paper
  44. Oliveira H., Sousa A., Alves A., Nogueira A.J.A., Santos C. (2012) Inoculation with Ophiostoma novo-ulmi subsp. americana affects photosynthesis, nutrition and oxidative stress in in vitro Ulmus minor plants. Environmental And Experimental Botany. 77, 146-155. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.envexpbot.2011.11.008
  45. Guimarães G., Cardoso L., Oliveira H., Santos C., Duarte P., Sottomayor M. (2012) Cytogenetic characterization and genome size of the medicinal plant Catharanthus roseus (L.) G. Don. Aob Plants. (see details) ISI paper
    Link: http://dx.doi.org/10.1093/aobpla/pls002
  46. Oliveira H., Lopes T., Almeida T., Pereira M.L., Santos C. (2012) Cadmium-induced genetic instability in mice testis. Human & Experimental Toxicology. 31, 12, 1228-1236. (see details) ISI paper
  47. Monteiro C., Santos C., Pinho S., Oliveira H., Pedrosa T., Dias M.C. (2012) Cadmium-induced cyto- and genotoxicity are organ-dependent in lettuce. Chemical Research In Toxicology. 25, 7, 1423-1434. (see details) ISI paper
    Link: http://pubs.acs.org/doi/full/10.1021/tx300039t
  48. Oliveira H., Santos C. (2011) AN INTEGRATIVE VIEW OF SODIUM CHLORIDE STRESS AND PHAEOMONIELLA SP. INOCULATION ON GROWTH AND NUTRIENT ACCUMULATION AND PATTERNING IN IN VITRO GRAPEVINE PLANTS. Journal Of Plant Nutrition. 34, 4, 557-572. (see details) ISI paper
  49. Oliveira H., Guevara M., Santos T., Santos C., Pereira M.L. (2010) Evaluation of in vivo reproductive toxicity of potassium chromate in male mice. Experimental And Toxicologic Pathology. 62, 391-404. (see details) ISI paper
    Link: http://dx.doi.org/10.1016/j.etp.2009.05.009
  50. Oliveira H, Barros A, delgadillo I, , Coimbra M, , Santos C (2009) Effects of fungi inoculation and salt stress on physiology and biochemistry of in vitro grapevines: emphasis on sugar composition changes by FT-IR analyses. Environmental And Experimental Botany. 65, 1, 1-10. (see details) ISI paper
    Link: doi:10.1016/j.envexpbot.2008.05.003
  51. Oliveira H., spano M., Santos C., Pereira M.L. (2009) Adverse effects of cadmium exposure on mouse sperm. Reproductive Toxicology. 28, 4, 550-559. (see details) ISI paper
    Link: http://www.ncbi.nlm.nih.gov/pubmed/19695322
  52. Oliveira H, Spanò M, Santos C, Pereira ML (2009) Lead chloride affects sperm motility and acrosome reaction in mice. Cell Biology And Toxicology . 25, 341-353. (see details) ISI paper
    Link: http://dx.doi.org/10.1007/s10565-008-9088-4
  53. Santos C., Fragoeiro S., Oliveira H., Phillips A. (2006) Response of Vitis vinifera L. plants inoculated with Phaeoacremonium angustius and Phaeomoniella chamydospora to thiabendazole, resveratrol and sodium arsenite. Scientia Horticulturae. 107, 131-136. (see details) ISI paper
    Link: doi:10.1016/j.scienta.2005.04.015
  54. Oliveira H., Santos T.M., Ramalho-Santos J., Pereira M.L. (2006) Histopathological effects of Hexavalent Chromium in Mice Kidney. Bulletin Of Environmental Contamination And Toxicology. 76, 977-983. (see details) ISI paper
    Link: DOI: 10.1007/s00128-006-1014-y
  55. Oliveira H., Loureiro J., Filipe L., Santos C., Ramalho-Santos J., Sousa M., Pereira M.L. (2006) Flow cytometry evaluation of lead and cadmium effects on mouse spermatogenesis. Reproductive Toxicology. 22, 529-535. (see details) ISI paper
    Link: http://dx.doi.org/10.1007/s00128-006-1014-y
  56. Pereira ML, Pires das Neves R., Oliveira H., Santos T.M., Pedrosa de Jesus J. (2005) Effect of Cr(V) on reproductive organ morphology and sperm parameters: An experimental study in mice. Environmental Health: A Global Access Science Source . 4, 9 (see details) ISI paper
    Link: http://www.ehjournal.net/content/4/1/9
  57. Santos C., Santos C, CV Santos, Falcão I., Falcão IP, Falcão IP, Pinto G, Pinto G, Pinto G., Oliveira H, Oliveira H., Oliveira H, Loureiro J, Loureiro J., Loureiro J (2002) Nutrient responses and glutamate and proline metabolism in sunflower plants and calli under Na2SO4 stress. Journal Of Plant Nutrition And Soil Science. 165, 366-372. (see details) ISI paper
  58. Santos C, Falcão IP, Pinto G, Oliveira H, Loureiro J (2002) Nutrient responses and glutamate and proline metabolism in sunflower plants and calli under Na2SO4 stress. Journal Of Plant Nutrition And Soil Science. 165, 326-372. (see details) ISI paper
    Link: http://dx.doi.org/10.1002/1522-2624(200206)165:3<366::AID-JPLN366>3.0.CO...
  1. Bastos V, Ferreira de Oliveira JMP, Duarte I, Santos C, Oliveira H (2015) Cytotoxicity of citrate and PEG coated AgNPs in human liver cells. Toxicol Lett. 238, (see details)
  2. Menezes AC, Ferreira de Oliveira JMP, Carvalheiro M, Oliveira H, Ascenso A (2015) Cytotoxicity of the Serotonergic Drug 1- (1-naphthyl) piperazine in Human MNT-1 Melanoma Cells. Toxicol Lett. 238, (see details)
  3. Pacheco ARC, Oliveira H, Santos C, Almeida L, Pimentel F, Ferreira de Oliveira JMP (2015) Cytotoxic activity of fisetin against the MG-63 osteosarcoma cell line. Toxicology Letters. 238, (see details)
  4. Rosário F, Reis C, Santos C, Oliveira H, Ferreira de Oliveira JMP (2014) Cyto and genotoxic effects of silver nanoparticles on A549 cell line. Toxicol Lett. 229, (see details)
  5. Coutinho L, Ferreira de Oliveira JMP, Oliveira H, Santos C (2014) Cytotoxic and genotoxic activity of hesperetin in an osteosarcoma in vitro model. Toxicol Lett. 229, (see details)
  6. Oliveira H. (2012) Chromium as an Environmental Pollutant: Insights on Induced Plant Toxicity. Journal Of Botany. 2012, Article ID 375843, 8 pages (see details)
    Link: http://doi:10.1155/2012/375843
  7. Santos C., Oliveira H., Deckert J. , White J.C. (2012) Legacy and Emerging Contaminants in Plants: From the Gene to the Field. Journal Of Botany. 2012, 2 (see details)
    Link: http://dx.doi.org/10.1155/2012/382717
  8. GUEVARA M. A., , OLIVEIRA H., , SILVA A., , PEREIRA M. L., MORGADO F. (2003) Segmentation and Morphometry of Histological Sections using Deformable Models: A New Tool for Evaluating Testicular Histopatology. Lecture Notes For Computer Sciences,. 2905, 282-290. (see details)
  1. R.B. Pinto, M- Nasirpour, J. Carrola, H.Oliveira, C.R.S. Freire. I.F. Duarte (2017) Antimicrobial Properties and Therapeutic Applicationsof Silver Nanoparticles. In: Alexandru Mihai Grumezescu. (EDS.), Antimicrobial Nanoarchitectonics 1st edition From Synthesis to Applications. Elsevier. 221 9780323527330. (see details)
  2. M.L. Pereira, H. Oliveira, H.M.A. Fonseca, F. Garcia e Costa, C. Santos. (2016) The Role of Cytometry for Male Fertility Assessment in Toxicology. In: Ingrid Schmid. (EDS.), Flow Cytometry - Select Topics. ISBN 978-953-51-2551-8. (see details)
    Link: http://www.intechopen.com/books/flow-cytometry-select-topics/the-role-o...
  3. Bastos V., Oliveira H., Rosário F., Remédios C., Ferreira de Oliveira J. M. P., Santos C. (2015) Chapter 77. Silver Nanoparticles: Potential hazard effects of silver nanoparticles to the environment and human health. In: Boris Ildusovich Kharisov, Oxana Vasilievna Kharissova, Ubaldo Ortiz-Mendez . (EDS.), CRC Consice Encyclopedia of Nanotechnology. CRC Press Taylor and Francis Group. 984-995. 978-1-4665-8034-3. (see details)
    Link: http://www.crcnetbase.com/doi/abs/10.1201/b19457-78
  4. Oliveira H, Spanò M, Santos C, Pereira ML (2009) Cytometric analyses of sperm functions and genetic integrity. In Male and Female Infertility: Genetic Causes, Hormonal Treatments and Health Effects. C (2010) Cytometric analyses of sperm functions and genetic integrity. In: Series Human Reproductive System - Anatomy, Roles and Disorder. (EDS.), In Male and Female Infertility: Genetic Causes, Hormonal Treatments and Health Effects. NY. chap1, 978-1-60876-654-3. (see details)
  1. Martins, Sandra Cristina Pinto (2015) Evaluation of circulating endothelial progenitor cells by multicolor flow cytometry in chronic kidney disease patients. MSc Thesis. [co-supervisor] (see details)
  2. Nasirpour, Maryam (2015) Synthesis and characterization of silver nanoparticles: a toxicity and metabolomics approach in skin cells. MSc Thesis. [supervisor] (see details)
  3. Reis C. S. (2013) Genotoxic effects of silver nanoparticles on lung cells. MSc Thesis. (see details)
  4. Oliveira H. (2009) Avaliação do efeito de metais pesados na fertilidade do ratinho. PhD Thesis. (see details)
    Link: http://ria.ua.pt/handle/10773/982
  5. Oliveira H. (2002) Estudo bioquímico de videiras (Vitis vinifera L.) cultivadas in vitro em ambiente salino e infectadas com Phaeomoniella chlamydospora. MSc Thesis. (see details)
  1. Ferreira V ., Pavlaki M ., Frankenbach S ., Frommlet J ., Saes R ., Martins R ., Maia F ., Tedim J ., Oliveira H ., Soares A ., Serodio J ., Loureiro S . (2017) Does encapsulation of the biocide DCOIT in silica nanocontainers reduces its toxicity? Effects on the photophysiology and cell cycle of the endosymbiotic algae Symbiodinium sp. (see details)
  2. Gonçalves A.C., Nora S.V., Castro S., Loureiro J., Oliveira H., Santos C., Silveira P. (2015) Towards a taxonomical revision of Calendula L. (Asteraceae) in the Iberian Peninsula. (see details)
  3. Bastos V, Ferreira de Oliveira JMP, Duarte I, Santos C, Oliveira H (2015) Cytotoxicity of citrate and PEG coated AgNPs in human liver cells. (see details)
  4. Menezes AC, Ferreira de Oliveira JMP, Carvalheiro M, Oliveira H, Ascenso A (2015) Cytotoxicity of the Serotonergic Drug 1-(1-naphthyl)piperazine in Human MNT-1 Melanoma Cells. (see details)
  5. Pacheco ARC, Oliveira H, Santos C, Almeida L, Pimentel F, Ferreira de Oliveira JMP (2015) Cytotoxic activity of fisetin against the MG-63 osteosarcoma cell line. (see details)
  6. Oliveira H (2015) Toxicity of Upconverting Nanoparticles. (see details)
  7. Oliveira H (2015) Assessing nanoparticle toxicity: overview of the effects of upconversion nanoparticles. (see details)
  8. Coutinho L, Ferreira de Oliveira JMP, Oliveira H, Santos C (2014) Cytotoxic and genotoxic activity of hesperetin in an osteosarcoma in vitro model. Toxicology Letters. (see details)
  9. Ferreira de Oliveira JMP, Oliveira H, Pinho S, Pimentel F, Almeida L, Van Zoelen EJJ, Santos C (2014) Cytotoxic and genotoxic activity of 3, 3', 4', 7-tetrahydroxy-flavone (fisetin) in an osteosarcoma in vitro model. Planta Medica. (see details)
  10. Pedrosa T, Ferreira de Oliveira JMP, Santos C, Duarte IF, Oliveira H (2014) Oxidative stress induced by AgNPs on human keratinocyte cells: the effect of the coating. (see details)
  11. Verónica Bastos, José Miguel P Ferreira de Oliveira, Iola F. Duarte, Conceição Santos and Helena Oliveira (2014) Silver Nanoparticles Cytotoxicity: Viability and Apoptotic effects to a Keratinocyte cell line. (see details)
  12. V. Bastos, T. Pedrosa, JMP Ferreira de Oliveira, IF. Duarte, C Santos, H Oliveira (2014) Cyto- and genotoxicity of silver nanoparticles in human keratinocytes: influence of surface coating. . (see details)
  13. Coutinho L., de Oliveira J.M.P.F., Oliveira H., Santos C. (2014) Cytotoxic and genotoxic activity of hesperetin in an osteosarcoma in vitro model. Toxicology Letters. 229, S157-S157. (see details) ISI paper
  14. Jarak I, Oliveira H, Ferreira de Oliveira JMP, Gil AM, Jones JG, Duarte IF (2014) NMR study of altered flux through pentose phosphate pathway in human cells exposed to silver nanoparticles. (see details)
  15. Rosário F, Reis C, Santos C, Oliveira H, Ferreira de Oliveira JMP (2014) Cyto and genotoxic effects of silver nanoparticles on A549 cell line. (see details)
  16. Oliveira H (2014) Measuring DNA damage in plant cells: applications in ecotoxicology. Invited communication. (see details)
  17. A. Ascenso, T. Pedrosa, S. Pinho, F. Pinho, J.M.P. Ferreira de Oliveira, H.C. Marques, H. Oliveira, S. Simões, C. Santos (2013) The Effect of UV-B Irradiation on Human Keratinocytes after Lycopene Exposure. (see details)
  18. T. Pedrosa, H. Oliveira, S. Pinho, C. Monteiro, C. Remédios, P. Pinto, M. Costa, S. Barros, J.M.P. Ferreira de Oliveira, A. Ascenso, S. Varanda, M. Santos, C. Santos (2013) Evaluation of reactive oxygen species production and mitochondrial membrane potential on adherent cell lines using flow cytometry. (see details)
  19. J. Carrola, V. Bastos, J.M.P. Ferreira de Oliveira, H. Oliveira, C. Santos, A.L. Silva, A.M. Gil, I.F. Duarte (2013) Estudo exploratório dos efeitos metabólicos de nanopartículas de prata (Ag-NPs) em queratinócitos da epiderme humana. (see details)
  20. V. Bastos, H. Oliveira., J.M.P. Ferreira de Oliveira, I.F. Duarte, C. Santos (2013) Evaluation of silver nanoparticles cytotoxicity and genotoxicity on a human keratinocyte cell line. (see details)
  21. Carrola J, Gil AM, Silva ALD, Bastos V, Oliveira H, Ferreira de Oliveira JMP, Santos C, Duarte IF (2013) Metabolic response of human keratinocytes to silver nanoparticles: a metabolomics study. (see details)
  22. Ascenso A, Pedrosa T, Pinho S, Pinho F, Ferreira de Oliveira JMP, Marques HC, Oliveira H, Simões S, Santos C (2013) The Effect of Lycopene Pre-Exposure on UV-B Irradiated Human Keratinocytes. (see details)
  23. Rosário F, , Oliveira H, Oliveira M, Santos C (2012) Evaluation of AgNP nanoparticles on osteosarcoma viability: approaches to environmental studies. (see details)
  24. Santos C., Oliveira M., Ascenso A., Remédios C., Costa M., Pinto P., Pinho S., Pinho F., Pedrosa T., Oliveira H. (2012) From the cytome to the genome: in vitro assays within the 3Rs perspective. (see details)
    Link: http://www.ua.pt/research/PageText.aspx?id=15254
  25. Remédios C., Costa M., Pinto P., Pinho F., Pedrosa T., Pinho S., Oliveira M., Oliveira H., Santos C. (2012) In vitro assessment of sulforaphane-induced genomic and nuclear damage in an osteosarcoma cell line. (see details)
  26. Costa M., Remédios C., Pinto P., Pinho F., Pedrosa T., Oliveira H., Oliveira M., Santos C. (2012) Cytotoxic effects induced by sulforaphane in human osteosarcoma cell line. (see details)
  27. Rosário F., Oliveira H., Oliveira M., Santos C. (2012) In Vitro assessment of the effects of silver nanoparticles on osteosarcoma viability: Uncoated vs Coated nanoparticles. (see details)
  28. Bastos V., Rosário F., Oliveira H., Oliveira M., Santos C. (2012) Toxicity assessment of uncoated Ag and TiO2 nanoparticles on human osteosarcoma cell lines. (see details)
  29. Rosário F., Bastos V., Oliveira H., Oliveira M., Santos C. (2012) Nanoparticles Interactions with Plants. (see details)
  30. Remédios C., Costa M., Pinto P., Pinho F, Pedrosa T., Pinho S., Oliveira M., Oliveira H., Santos C. (2012) Genotoxic activity of sulforaphane in an osteosarcoma in vitro model. (see details)
  31. Costa M., Remédios C., Pinto P., Pedrosa T., Pinho F., Pinho S., Oliveira H., Oliveira M., Santos C. (2012) Sulforaphane induced oxidative stress in human osteosarcoma cell line. (see details)
  32. S. Pinho, J.M. Oliveira, H. Oliveira, and C. Santos (2012) Plant compounds as an alternative to chemotherapy treatment in human osteosarcoma. (see details)
  33. Santos C, Oliveira H, Ferreira de Oliveira JMP (2012) The biotechnological potentials of in vitro toxicological tests: case studies. (see details)
  34. Ferreira de Oliveira JMP, Costa M, Pedrosa T, Pinto P, Oliveira H, Pimentel F, Almeida L, Santos C (2012) Oxidative stress modulation by sulforaphane in MG-63 cells. (see details)
  35. Bastos V., Vilhena H., Miranda S., Pinho S., Teixeira J.P., Santos C., Nogueira A.J.A., Oliveira H., Sousa A.C.A. (2011) DNA damage in european pets assessed by comet assay. (see details)
  36. Monteiro C., Oliveira H., Rodriguez E., Pedrosa T., Dias M.C., Santos C. (2011) Cadmium effects in lettuce: genotoxicity, protein oxidation, lipid peroxidation and cell cycle alterations. (see details)
  37. Ascenso A., Pedrosa T., Oliveira H., Cabral Marques H., Santos C. (2011) The effect of UV-B radiation on HaCat cell viability and proliferation. (see details)
  38. Ascenso A., Pedrosa T., Oliveira H., Cabral Marques H., Santos C., Simões S. (2011) Evaluation of in vitro and in vivo toxicity of tretinoin loaded in ultradeformable liposomes. (see details)
  39. Pinho F., Oliveira H., Santos C. (2011) Cytotoxic and genotoxic effects of cadmium exposure in human osteoblasts. (see details)
  40. Pinto P., Barros S., Oliveira H., Santos C. (2011) Cytostatic and genotoxic activity of sulforaphane in human osteosarcoma lines. (see details)
  41. Pedrosa T., Pinho F., Oliveira H., Santos C. (2011) Assessment of cadmium induced cytotoxicity and apopotosis on human Osteoblast-Like Cell Line MG-63. (see details)
  42. Oliveira H., Santos C. (2010) Chromium exposure induced cell cycle arrest in human osteoblasts. (see details)