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title Secretome analysis identifies potential virulence factors of Diplodia corticola, a fungal pathogen involved in cork oak (Quercus suber) decline
authors Fernandes, I; Alves, A; Correia, A; Devreese, B; Esteves, AC
author full name Fernandes, Isabel; Alves, Artur; Correia, Antonio; Devreese, Bart; Esteves, Ana Cristina
title Secretome analysis identifies potential virulence factors of Diplodia corticola, a fungal pathogen involved in cork oak (Quercus suber) decline
nationality internacional
source FUNGAL BIOLOGY
language English
document type Article
author keywords 2D-electrophoresis; Botryosphaeriaceae; Filamentous fungi; Plant pathogen; Quercus suber
keywords plus BOTRYTIS-CINEREA SECRETOME; PROTEOMIC ANALYSIS; STREPTOCOCCUS-PNEUMONIAE; MAGNAPORTHE-ORYZAE; SOUTH-AFRICA; CELL WALL; SCLEROTINIA-SCLEROTIORUM; MACROPHOMINA-PHASEOLINA; EXTRACELLULAR PROTEINS; CHEMICAL-COMPOSITION
abstract The characterisation of the secretome of phytopathogenic fungi may contribute to elucidate the molecular mechanisms of pathogenesis. This is particularly relevant for Diplodia corticola, a fungal plant pathogen belonging to the family Botryosphaeriaceae, whose genome remains unsequenced. This phytopathogenic fungus is recognised as one of the most important pathogens of cork oak, being related to the decline of cork oak forests in the Iberian Peninsula. Unfortunately, secretome analysis of filamentous fungi is limited by the low protein concentration and by the presence of many interfering substances, such as polysaccharides, which affect the separation and analysis by 1D and 2D gel electrophoresis. We compared six protein extraction protocols concerning their suitability for further application with proteomic workflows. The protocols involving protein precipitation were the most efficient, with emphasis on TCA acetone protocol, allowing us to identify the most abundant proteins on the secretome of this plant pathogen. Approximately 60% of the spots detected were identified, all corresponding to extracellular proteins. Most proteins identified were carbohydrate degrading enzymes and proteases that may be related to D. corticola pathogenicity. Although the secretome was assessed in a noninfection environment, Potential virulence factors such as the putative glucan beta-glucosidase, neuraminidase, and the putative ferulic acid esterase were identified. The data obtained forms a useful basis for a deeper understanding of the pathogenicity and infection biology of D. corticola. Moreover, it will contribute to the development of proteomics studies on other members of the Botryosphaeriaceae. (C) 2014 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
author address [Fernandes, Isabel; Alves, Artur; Correia, Antonio; Esteves, Ana Cristina] Univ Aveiro, CESAM, Dept Biol, P-3810193 Aveiro, Portugal; [Fernandes, Isabel; Devreese, Bart] Univ Ghent, Dept Biochem Physiol & Microbiol, Lab Prot Biochem & Prot Engn, B-9000 Ghent, Belgium
reprint address Esteves, AC (reprint author), Univ Aveiro, CESAM, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address acesteves@ua.pt
researcherid number Esteves, Ana Cristina/B-2939-2008; Alves, Artur/C-3645-2008; Correia, Antonio/B-1593-2008
orcid number Esteves, Ana Cristina/0000-0003-2239-2976; Alves, Artur/0000-0003-0117-2958; Correia, Antonio/0000-0002-5115-1429
funding agency and grant number FEDER through COMPETE program; FCT within the research project PROMETHEUS [PTDC/AGR-CFL/113831/2009, FCOMP-01-0124-FEDER-014096]; Pest [PEst-C/MAR/LA0017/2013]; FCT [BPD/38008/2007, BD/66223/2009]
funding text This study was supported by FEDER funding through COMPETE program and by national funding through FCT within the research project PROMETHEUS (PTDC/AGR-CFL/113831/2009 and FCOMP-01-0124-FEDER-014096) and Pest (PEst-C/MAR/LA0017/2013). FCT also financed AC Esteves (BPD/38008/2007) and I Fernandes (BD/66223/2009).
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cited reference count 72
publisher ELSEVIER SCI LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, OXON, ENGLAND
issn 1878-6146
29-character source abbreviation FUNGAL BIOL-UK
iso source abbreviation Fungal Biol.
publication date MAY-JUN
year published 2014
volume 118
issue 5-6
beginning page 516
ending page 523
digital object identifier (doi) 10.1016/j.funbio.2014.04.006
page count 8
web of science category Mycology
subject category Mycology
document delivery number AJ7NF
unique article identifier WOS:000337882700009
link http://dx.doi.org/10.1016/j.funbio.2014.04.006