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title Biochemical and standard toxic effects of acetaminophen on the macrophyte species Lemna minor and Lemna gibba
authors Nunes, B; Pinto, G; Martins, L; Goncalves, F; Antunes, SC
author full name Nunes, Bruno; Pinto, Gloria; Martins, Liliana; Goncalves, Fernando; Antunes, Sara C.
title Biochemical and standard toxic effects of acetaminophen on the macrophyte species Lemna minor and Lemna gibba
nationality internacional
source ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
language English
document type Article
author keywords Aquatic plants; Pharmaceutical drugs; Paracetamol; Oxidative stress; Biomarkers
keywords plus SEWAGE-TREATMENT PLANTS; PERSONAL CARE PRODUCTS; SURFACE WATERS; AQUATIC PLANTS; HUMAN PHARMACEUTICALS; ENVIRONMENTAL RISKS; PROLINE METABOLISM; GAMBUSIA-HOLBROOKI; OXIDATIVE STRESS; DRINKING-WATER
abstract Acetaminophen is globally one of the most prescribed drugs due to its antipyretic and analgesic properties. However, it is highly toxic when the dosage surpasses the detoxification capability of an exposed organism, with involvement of an already described oxidative stress pathway. To address the issue of the ecotoxicity of acetaminophen, we performed acute exposures of two aquatic plant species, Lemna gibba and Lemna minor, to this compound. The selected biomarkers were number of fronds, biomass, chlorophyll content, lipid peroxidation (TBARS assay), and proline content. Our results showed marked differences between the two species. Acetaminophen caused a significant decrease in the number of fronds (EC50 = 446.6 mg/L), and the establishment of a dose-dependent peroxidative damage in L. minor, but not in L. gibba. No effects were reported in both species for the indicative parameters chlorophyll content and total biomass. However, the proline content in L. gibba was substantially reduced. The overall conclusions point to the occurrence of an oxidative stress scenario more prominent for L. minor. However, the mechanisms that allowed L. gibba to cope with acetaminophen exposure were distinct from those reported for L. minor, with the likely involvement of proline as antioxidant.
author address [Nunes, Bruno; Pinto, Gloria; Goncalves, Fernando; Antunes, Sara C.] Univ Aveiro, Ctr Estudos Ambiente & Mar CESAM, P-3810193 Aveiro, Portugal; [Nunes, Bruno; Pinto, Gloria; Goncalves, Fernando] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Martins, Liliana] Univ Fernando Pessoa, Fac Ciencias Saude, Oporto, Portugal; [Antunes, Sara C.] Univ Porto, Fac Ciencias, Dept Biol, P-4169007 Oporto, Portugal
reprint address Nunes, B (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address nunes.b@ua.pt
researcherid number Goncalves, Fernando/B-8000-2008
orcid number Goncalves, Fernando/0000-0002-9326-187X
funding agency and grant number European Funds through COMPETE; National Funds through the Portuguese Science Foundation (FCT) [PEst-C/MAR/LA0017/2013]; Human Potential Operational Programme (National Strategic Reference Framework); European Social Fund (EU)
funding text This work was supported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013. Gloria Pinto was hired under the programme Ciencia 2008 (FCT, Portugal) and Bruno Nunes under the programme Investigador FCT co-funded by the Human Potential Operational Programme (National Strategic Reference Framework 2007-2013) and European Social Fund (EU).
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cited reference count 63
publisher SPRINGER HEIDELBERG
publisher city HEIDELBERG
publisher address TIERGARTENSTRASSE 17, D-69121 HEIDELBERG, GERMANY
issn 0944-1344
29-character source abbreviation ENVIRON SCI POLLUT R
iso source abbreviation Environ. Sci. Pollut. Res.
publication date SEP
year published 2014
volume 21
issue 18
beginning page 10815
ending page 10822
digital object identifier (doi) 10.1007/s11356-014-3059-5
page count 8
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number AP5QA
unique article identifier WOS:000342132500025
link http://dx.doi.org/10.1007/s11356-014-3059-5