HOME  »  Members  »  Integrated  »  Post-Docs
title Sensitivity of the sea snail Gibbula umbilicalis to mercury exposure - Linking endpoints from different biological organization levels
authors Cabecinhas, AS; Novais, SC; Santos, SC; Rodrigues, ACM; Pestana, JLT; Soares, AMVM; Lemos, MFL
author full name Cabecinhas, Adriana S.; Novais, Sara C.; Santos, Silvia C.; Rodrigues, Andreia C. M.; Pestana, Joao L. T.; Soares, Amadeu M. V. M.; Lemos, Marco F. L.
title Sensitivity of the sea snail Gibbula umbilicalis to mercury exposure - Linking endpoints from different biological organization levels
nationality internacional
language English
document type Article
author keywords Biomarkers; Cholinesterase; Behavior; Marine ecotoxicology; Post-exposure feeding
abstract Mercury contamination is a common phenomenon in the marine environment and for this reason it is important to develop cost-effective and relevant tools to assess its toxic effects on a number of different species. To evaluate the possible effects of Hg in the sea snail Gibbula umbilicalis, animals were exposed to increasing concentrations of the contaminant in the ionic form for 96 h. After this exposure period, mortality, feeding and flipping behavior, the activity of the biomarkers glutathione S-transferase, superoxide dismutase, catalase, lactate dehydrogenase and cholinesterase, the levels of lipid peroxidation and cellular energy allocation were measured. After 96 h of exposure to the highest Hg concentration (approximate to LC20), there was a significant inhibition of the cholinesterase activity as well as impairment in the flipping behavior and post-exposure feeding of the snails. Cholinesterase inhibition was correlated with the impairment of behavioral responses also caused by exposure to Hg. These endpoints, including the novel flipping test, revealed sensitivity to Hg and might be used as relevant early warning indicators of prospective effects at higher biological organization levels, making these parameters potential tools for environmental risk assessment. The proposed test species showed sensitivity to Hg and proved to be a suitable and resourceful species to be used in ecotoxicological testing to assess effects of other contaminants in marine ecosystems. (C) 2014 Elsevier Ltd. All rights reserved.
author address [Cabecinhas, Adriana S.; Novais, Sara C.; Lemos, Marco F. L.] Polytech Inst Leiria, GIRM, ESTM, P-2520641 Peniche, Portugal; [Novais, Sara C.; Santos, Silvia C.; Rodrigues, Andreia C. M.; Pestana, Joao L. T.; Soares, Amadeu M. V. M.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Novais, Sara C.; Santos, Silvia C.; Rodrigues, Andreia C. M.; Pestana, Joao L. T.; Soares, Amadeu M. V. M.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal
reprint address Lemos, MFL (reprint author), Escola Super Turismo & Tecnol Mar, P-2520641 Peniche, Portugal.
e-mail address marco.lemos@ipleiria.pt
researcherid number Soares, Amadeu/A-8304-2008
orcid number Soares, Amadeu/0000-0003-0879-9470
funding agency and grant number Fundacao para a Ciencia e Tecnologia [SFRH/BPD/94500/2013, SFRH/BPD/45342/2008]
funding text S.C. Novais and J.L.T. Pestana wish to acknowledge the financial support given by Fundacao para a Ciencia e Tecnologia (SFRH/BPD/94500/2013 and SFRH/BPD/45342/2008, respectively). Authors would like to thank Joao Branco, Carina Marques, Ines Pimparel, and Sara Marques for their assistance in laboratory and field work.
cited references Azevedo-Pereira HMVS, 2011, ECOTOX ENVIRON SAFE, V74, P1210, DOI 10.1016/j.ecoenv.2011.03.018; Bainy Afonso Celso Dias, 2006, Biotemas, V19, P35; Bensefa-Colas L, 2011, REV MED INTERNE, V32, P416, DOI 10.1016/j.revmed.2009.08.024; BLOOM NS, 1983, MAR CHEM, V14, P49, DOI 10.1016/0304-4203(83)90069-5; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1006/abio.1976.9999; Brambila E, 2002, J TOXICOL ENV HEAL A, V65, P1273, DOI 10.1080/00984100290071405; Callil CT, 2001, WATER AIR SOIL POLL, V125, P319, DOI 10.1023/A:1005230716898; Cardoso PG, 2012, WATER AIR SOIL POLL, V223, P421, DOI 10.1007/s11270-011-0870-1; Clairborne A., 1985, CRC HDB METHODS OXYG; CLARK AG, 1986, PESTIC BIOCHEM PHYS, V25, P169, DOI 10.1016/0048-3575(86)90044-1; Clarkson TW, 2003, NEW ENGL J MED, V349, P1731, DOI 10.1056/NEJMra022471; Costa S, 2011, WATER AIR SOIL POLL, V217, P689, DOI 10.1007/s11270-010-0620-9; Costley CT, 2000, ANAL CHIM ACTA, V405, P179, DOI 10.1016/S0003-2670(99)00742-4; Cunha I, 2007, COMP BIOCHEM PHYS C, V145, P648, DOI 10.1016/j.cbpc.2007.02.014; De Coen W. M., 1997, Journal of Aquatic Ecosystem Stress and Recovery, V6, P43, DOI 10.1023/A:1008228517955; De Coen WM, 2003, ENVIRON TOXICOL CHEM, V22, P1632, DOI 10.1897/1551-5028(2003)22<1632:TMBLRB>2.0.CO;2; De Coen WM, 2001, ECOTOX ENVIRON SAFE, V48, P223, DOI 10.1006/eesa.2000.2009; Diamantino TC, 2001, CHEMOSPHERE, V45, P553, DOI 10.1016/S0045-6535(01)00029-7; EISLER R, 1977, ARCH ENVIRON CON TOX, V6, P315; Elia A.C., 2003, ECOTOX ENVIRON SAFE, V55, P167; ELLMAN GL, 1961, BIOCHEM PHARMACOL, V7, P88, DOI 10.1016/0006-2952(61)90145-9; Engenheiro EL, 2005, ENVIRON TOXICOL CHEM, V24, P603, DOI 10.1897/04-131R.1; Ercal Nuran, 2001, Current Topics in Medicinal Chemistry, V1, P529, DOI 10.2174/1568026013394831; Erk M, 2012, J SHELLFISH RES, V31, P61, DOI 10.2983/035.031.0108; ESTERBAUER H, 1991, FREE RADICAL BIO MED, V11, P81, DOI 10.1016/0891-5849(91)90192-6; Ferrat L, 2002, B MAR SCI, V71, P1183; Finney D.J., 1971, PROBIT ANAL; Fowler S.W., 1991, MAR ENVIRON RES, V29, P1; Frasco MF, 2007, FEBS J, V274, P1849, DOI 10.1111/j.1742-4658.2007.05732.x; Frasco MF, 2002, FISH PHYSIOL BIOCHEM, V26, P149, DOI 10.1023/A:1025457831923; Gravato C., 2010, ECOTOXICOL ENV SAFET, V73; Guilhermino L, 1996, CHEMOSPHERE, V32, P727, DOI 10.1016/0045-6535(95)00360-6; Habig W H, 1981, Methods Enzymol, V77, P398; HELCOM, 1987, BALT SEA ENV P, V24; Houston MC, 2011, J CLIN HYPERTENS, V13, P621, DOI 10.1111/j.1751-7176.2011.00489.x; Howcroft CF, 2011, ECOTOXICOLOGY, V20, P119, DOI 10.1007/s10646-010-0562-4; Jakka NM, 2007, DRUG CHEM TOXICOL, V30, P383, DOI 10.1080/01480540701522551; KRISHNAJA AP, 1987, MAR ENVIRON RES, V21, P109, DOI 10.1016/0141-1136(87)90045-6; Lemos MFL, 2010, PROTEOMICS, V10, P873, DOI 10.1002/pmic.200900470; Lima I, 2007, CHEMOSPHERE, V66, P1230, DOI 10.1016/j.chemosphere.2006.07.057; MCCORD JM, 1969, J BIOL CHEM, V244, P6049; Mellergard J, 2004, TOXICOLOGY, V196, P197, DOI 10.1016/j.tox.2003.10.004; Mirzoian A, 2002, J PHARMACOL EXP THER, V302, P560, DOI 10.1124/jpet.102.035154; Monteiro DA, 2010, ECOTOXICOLOGY, V19, P105, DOI 10.1007/s10646-009-0395-1; Monteiro M, 2007, CHEMOSPHERE, V66, P514, DOI 10.1016/j.chemosphere.2006.05.061; Nguyen T.H.L., 1997, THESIS U GHENT GHENT; O'Driscoll NJ, 2007, ENVIRON SCI TECHNOL, V41, P5342, DOI 10.1021/es070147r; Portmann J., 1971, SHELL FISH INFORM, V22, P1; RAY GL, 1976, J ENVIRON QUAL, V5, P193; Ribeiro S, 1999, ECOTOX ENVIRON SAFE, V44, P287, DOI 10.1006/eesa.1999.1837; Richetti SK, 2011, NEUROTOXICOLOGY, V32, P116, DOI 10.1016/j.neuro.2010.11.001; Rodgers JS, 2001, BIOCHEM PHARMACOL, V61, P1543, DOI 10.1016/S0006-2952(01)00629-3; Samson C.J., 2001, AQUAT TOXICOL, V51, P369; Sarkar A., 2011, ENV POLLUTION ECOLOG, P100; Scheuhammer AM, 2008, ECOTOXICOLOGY, V17, P93, DOI 10.1007/s10646-007-0170-0; Sioen I, 2008, CHEMOSPHERE, V71, P1056, DOI 10.1016/j.chemosphere.2007.11.025; Smith GM, 1997, J EXP MAR BIOL ECOL, V209, P75, DOI 10.1016/S0022-0981(96)02590-7; Smolders R, 2004, ENVIRON POLLUT, V129, P99, DOI 10.1016/j.envpol.2003.09.027; Stein ED, 1996, CRIT REV ENV SCI TEC, V26, P1, DOI 10.1080/10643380490265117; Taylor P., 2012, J ENV SCI HLTH, V46, P1129; Triebskorn Rita, 2009, Umweltwissenschaften und Schadstoff-Forschung, V21, P140, DOI 10.1007/s12302-009-0042-8; Valega M, 2009, CHEMOSPHERE, V74, P530, DOI 10.1016/j.chemosphere.2008.09.076; Vassault A., 1983, METHOD ENZYMAT AN, VIII, P18; Verslycke T, 2004, J EXP MAR BIOL ECOL, V306, P1, DOI 10.1016/j.jembe.2003.12.022; Verslycke T, 2004, J EXP MAR BIOL ECOL, V306, P245, DOI 10.1016/j.jembe.2004.01.014; Vieira LR, 2009, CHEMOSPHERE, V76, P1416, DOI 10.1016/j.chemosphere.2009.06.005; WILLIAMS EE, 1964, J ANIM ECOL, V33, P433, DOI 10.2307/2563; Zalups RK, 2000, PHARMACOL REV, V52, P113
cited reference count 68
publisher city OXFORD
issn 0045-6535
29-character source abbreviation CHEMOSPHERE
iso source abbreviation Chemosphere
publication date JAN
year published 2015
volume 119
beginning page 490
ending page 497
digital object identifier (doi) 10.1016/j.chemosphere.2014.07.016
page count 8
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number AY7KQ
unique article identifier WOS:000347739600068
link http://dx.doi.org/10.1016/j.chemosphere.2014.07.016