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title SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer
authors Costa, L; Esteves, AC; Correia, A; Moreirinha, C; Delgadillo, I; Cunha, A; Neves, MGPS; Faustino, MAF; Almeida, A
author full name Costa, Liliana; Esteves, Ana Cristina; Correia, Antonio; Moreirinha, Catarina; Delgadillo, Ivonne; Cunha, Angela; Neves, Maria G. P. S.; Faustino, Maria A. F.; Almeida, Adelaide
title SDS-PAGE and IR spectroscopy to evaluate modifications in the viral protein profile induced by a cationic porphyrinic photosensitizer
nationality internacional
language English
document type Article
author keywords Photodynamic inactivation; Porphyrin; ROS; Bacteriophages; SOS-PAGE; IR spectroscopy
abstract Reactive oxygen species can be responsible for microbial photodynamic inactivation due to its toxic effects, which include severe damage to proteins, lipids and nucleic acids. In this study, the photo-oxidative modifications of the proteins of a non-enveloped T4-like bacteriophage, induced by the cationic porphyrin 5,10,15-tris(1-methylpyridinium-4-yl)-20-(pentafluorophenyl)porphyrin tri-iodide were evaluated. Two methods were used: sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) and infrared spectroscopy. SDS-PAGE analysis showed that the phage protein profile was considerably altered after photodynamic treatment. Seven protein bands putatively corresponding to capsid and tail tube proteins were attenuated and two other were enhanced. Infrared spectroscopy confirmed the time-dependent alteration on the phage protein profile detected by SDS-PAGE, indicative of a response to oxidative damage. Infrared analysis showed to be a promising and rapid screening approach for the analysis of the modifications induced on viral proteins by photosensitization. In fact, one single infrared spectrum can highlight the changes induced to all viral molecular structures, overcoming the delays and complex protocols of the conventional methods, in a much simple and cost effective way. (C) 2014 Elsevier B.V. All rights reserved.
author address [Costa, Liliana; Esteves, Ana Cristina; Correia, Antonio; Moreirinha, Catarina; Cunha, Angela; Almeida, Adelaide] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Costa, Liliana; Esteves, Ana Cristina; Correia, Antonio; Moreirinha, Catarina; Cunha, Angela; Almeida, Adelaide] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Moreirinha, Catarina; Delgadillo, Ivonne; Neves, Maria G. P. S.; Faustino, Maria A. F.] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal; [Moreirinha, Catarina; Delgadillo, Ivonne; Neves, Maria G. P. S.; Faustino, Maria A. F.] Univ Aveiro, QOPNA, P-3810193 Aveiro, Portugal
reprint address Almeida, A (reprint author), Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal.
e-mail address aalmeida@ua.pt
researcherid number Correia, Antonio/B-1593-2008; Almeida, Adelaide/D-9899-2011; Esteves, Ana Cristina/B-2939-2008
orcid number Correia, Antonio/0000-0002-5115-1429; Almeida, Adelaide/0000-0002-8422-8664; Esteves, Ana Cristina/0000-0003-2239-2976
funding agency and grant number CESAM [PEst-Pest-C/MAR/LA0017/2013]; FCT [BD/39906/2007, BPD-38008/2007]; University of Aveiro; Fundacao para a Ciencia e a Tecnologia; FEDER [PEst-C/QUI/UI0062/2013, FCOMP-01-0124-FEDER-037296]
funding text Thanks are due to the University of Aveiro, Fundacao para a Ciencia e a Tecnologia and FEDER for funding the QOPNA unit (project PEst-C/QUI/UI0062/2013, FCOMP-01-0124-FEDER-037296) and to CESAM for funding the Microbiology Research Group unit (project PEst-Pest-C/MAR/LA0017/2013). Liliana Costa and Ana Cristina Esteves are grateful to FCT for their grants (BD/39906/2007 and BPD-38008/2007).
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cited reference count 53
publisher city AMSTERDAM
publisher address PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
issn 0166-0934
29-character source abbreviation J VIROL METHODS
iso source abbreviation J. Virol. Methods
publication date DEC 1
year published 2014
volume 209
beginning page 103
ending page 109
digital object identifier (doi) 10.1016/j.jviromet.2014.09.013
page count 7
web of science category Biochemical Research Methods; Biotechnology & Applied Microbiology; Virology
subject category Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Virology
document delivery number AR8TQ
unique article identifier WOS:000343847600016