HOME  »  Members  »  Integrated  »  Post-Docs
 
title From north to south and back: the role of the Balkans and other southern peninsulas in the recolonization of Europe by wild boar
authors Velickovic, N; Djan, M; Ferreira, E; Stergar, M; Obreht, D; Maletic, V; Fonseca, C
author full name Velickovic, Nevena; Djan, Mihajla; Ferreira, Eduardo; Stergar, Matija; Obreht, Dragana; Maletic, Vladimir; Fonseca, Carlos
title From north to south and back: the role of the Balkans and other southern peninsulas in the recolonization of Europe by wild boar
nationality internacional
source JOURNAL OF BIOGEOGRAPHY
language English
document type Article
author keywords Balkan Peninsula; Europe; genetic variability; mtDNA; phylogeography; post-glacial recolonization; Sus scrofa; wild boar
keywords plus POPULATION GENETIC-STRUCTURE; HARE LEPUS-EUROPAEUS; SUS-SCROFA; PIG DOMESTICATION; ANCIENT DNA; PHYLOGEOGRAPHY; COLONIZATION; TRANSLOCATIONS; CONSEQUENCES; QUATERNARY
abstract AimWe analysed mitochondrial DNA (mtDNA) variation in wild boar (Sus scrofa) in the Balkans, including individuals from the northern Dinaric Balkans, an area that had not previously been characterized. Our aims were: (1) to reveal the level of genetic diversity and structuring and examine the demographic expansion of wild boar populations in the Balkans and Europe; (2) to examine the role of the Balkan gene pool in the post-LGM (Last Glacial Maximum) recolonization of Europe; and (3) to elucidate the phylogenetic position of European and Balkan wild boar in a Eurasian context by comparing sequences of wild boar worldwide. LocationBalkan Peninsula. MethodsA fragment of the mtDNA control region (443bp) was sequenced in 163 wild boar from the Balkans. Phylogenetic analyses, using MrBayes and network, were carried out together with 188 previously published sequences from the Balkan Peninsula. Phylogenetic analyses were also performed with an additional 876 wild boar sequences from around the world. ResultsSixteen haplotypes were found in the new samples, including 11 not previously reported in the Balkans. Phylogenetic analyses based on all known Balkan haplotypes indicated the existence of population structuring, revealing two groups: Continental Balkans and South Balkans. The analysis of the complete dataset, comprising 1227 mtDNA sequences from wild boar sampled worldwide, revealed the presence of 168 different haplotypes. All Balkan haplotypes fell into the E1 haplogroup, except one sample that possessed an Asian haplotype. Within the E1 haplogroup, 50% of the haplotypes were unique to the Balkan Peninsula. Main conclusionsWild boar from the Balkans exhibited high genetic diversity. Similar phylogeographical patterns emerge in all southern European peninsulas, arising from post-LGM expansion, and all three peninsulas played a similar role in the post-glacial recolonization of Europe by wild boar. This supports a leading-edge colonization hypothesis for all three peninsulas.
author address [Velickovic, Nevena; Djan, Mihajla; Obreht, Dragana] Univ Novi Sad, Dept Biol & Ecol, Fac Sci, Novi Sad 21000, Serbia; [Ferreira, Eduardo; Fonseca, Carlos] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Ferreira, Eduardo; Fonseca, Carlos] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Stergar, Matija] Univ Ljubljana, Biotech Fac, Dept Forestry & Renewable Forest Resources, Ljubljana 1000, Slovenia; [Maletic, Vladimir] Ss Cyril & Methodius Univ Skopje, Fac Forestry, Skopje 1000, Macedonia
reprint address Velickovic, N (reprint author), Univ Novi Sad, Dept Biol & Ecol, Fac Sci, Trg Dositeja Obradovica 2, Novi Sad 21000, Serbia.
e-mail address nevena.velickovic@dbe.uns.ac.rs
researcherid number Ferreira, Eduardo/G-2291-2010; CESAM, UA/M-3762-2015
orcid number Ferreira, Eduardo/0000-0003-0497-6118;
funding agency and grant number Ministry of Education, Science and Technological Development, Republic of Serbia [43002]; Provincial Secretariat for Science and Technological Development [114-457-2173/2011-01]; Provincial Secretariat for Agriculture, Water Management and Forestry Project 'The estimation of genetic potential of wild boar in hunting areas in Vojvodina'; FCT (Program POPHQREN) [SFRH/BPD/72895/2010]; European Funds through COMPETE; National Funds through the Portuguese Science Foundation (FCT) [PEst-C/MAR/LA0017/2013]; FEBS Collaborative Experimental Scholarship for Central and Eastern Europe
funding text This work was financially supported by the Ministry of Education, Science and Technological Development, Republic of Serbia, Grant No. 43002, the Provincial Secretariat for Science and Technological Development, Grant No. 114-457-2173/2011-01, and the Provincial Secretariat for Agriculture, Water Management and Forestry Project 'The estimation of genetic potential of wild boar in hunting areas in Vojvodina'. We thank all those who helped us in collecting samples: Mladen Gagrcin (Serbia); Milan Stevanovic (Serbia); Zoran Rasic (Serbia); Dragan Rogozarski (Serbia); Vlastimir Adamovic (Serbia); Ranko Savic (Montenegro); Darko Cvijovic (Montenegro); Veterinary Ambulance Niksic (Montenegro); Albert Marinculic (Croatia); Ivona Panic (Bosnia and Herzegovina); the public company 'Vojvodinasume' Novi Sad (Serbia); and several local hunters and veterinarians. Eduardo Ferreira was supported by a post-doctoral grant from FCT (Program POPHQREN, ref: SFRH/BPD/72895/2010). This work was cosupported by European Funds through COMPETE and by National Funds through the Portuguese Science Foundation (FCT) within project PEst-C/MAR/LA0017/2013. Nevena Velickovic was supported by FEBS Collaborative Experimental Scholarship for Central and Eastern Europe. We thank the editors Jonathan P. Sadler and Liliana Katinas, and four anonymous referees for their valuable comments and constructive suggestions on the manuscript.
cited references Alexandri P, 2012, J BIOGEOGR, V39, P713, DOI 10.1111/j.1365-2699.2011.02636.x; Alves E, 2003, ANIM GENET, V34, P319, DOI 10.1046/j.1365-2052.2003.01010.x; Alves PC, 2010, BIOL J LINN SOC, V101, P797, DOI 10.1111/j.1095-8312.2010.01530.x; APOLLONIO M, 1988, B ZOOL, V55, P213; Apollonio M, 2010, EUROPEAN UNGULATES T; Bandelt HJ, 1999, MOL BIOL EVOL, V16, P37; Birungi J, 2000, MOL ECOL, V9, P1997, DOI 10.1046/j.1365-294X.2000.01107.x; Briedermann L., 1990, SCHWARZWILD; Drummond AJ, 2005, MOL BIOL EVOL, V22, P1185, DOI 10.1093/molbev/msi103; Drummond AJ, 2012, MOL BIOL EVOL, V29, P1969, DOI 10.1093/molbev/mss075; Excoffier L, 2010, MOL ECOL RESOUR, V10, P564, DOI 10.1111/j.1755-0998.2010.02847.x; Fang M, 2006, ANIM GENET, V37, P459, DOI 10.1111/j.1365-2052.2006.01498.x; Ferreira E., 2006, Wildlife Biology in Practice, V2, P17; Ferreira E, 2009, MAMM BIOL, V74, P274, DOI 10.1016/j.mambio.2008.05.009; Fonseca C., 2004, THESIS U AVEIRO PORT; Giuffra E, 2000, GENETICS, V154, P1785; Hewitt G, 2000, NATURE, V405, P907, DOI 10.1038/35016000; Hewitt GM, 2004, PHILOS T ROY SOC B, V359, P183, DOI 10.1098/rstb.2003.1388; Hewitt GM, 1999, BIOL J LINN SOC, V68, P87, DOI 10.1006/bijl.1999.0332; Kasapidis P, 2005, MOL PHYLOGENET EVOL, V34, P55, DOI 10.1016/j.ympev.2004.09.007; Kusza S, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0091401; Larson G, 2005, SCIENCE, V307, P1618, DOI 10.1126/science.1106927; Larson G, 2007, P NATL ACAD SCI USA, V104, P15276, DOI 10.1073/pnas.0703411104; Linnell John D.C., 2011, P12; Massei G, 2000, IL CINGHIALE; Nikolov IS, 2009, ACTA THERIOL, V54, P193, DOI 10.4098/j.at.0001-7051.049.2008; Oliver W., 2008, SUS SCROFA IUCN RED; Ottoni C, 2013, MOL BIOL EVOL, V30, P824, DOI 10.1093/molbev/mss261; Rambaut A, 2014, FIGTREE VERSION 1 4; Rambaut A., 2014, TRACER V1 6; Randi E, 2005, VET RES COMMUN, V29, P71, DOI 10.1007/s11259-005-0025-1; Randi E, 1998, P ROY SOC B-BIOL SCI, V265, P793; Ronquist F, 2012, SYST BIOL, V61, P539, DOI 10.1093/sysbio/sys029; Sambrook J., 2001, MOL CLONING LAB MANU; Scandura M, 2008, MOL ECOL, V17, P1745, DOI 10.1111/j.1365-294X.2008.03703.x; Scandura M, 2011, MAMMAL REV, V41, P125, DOI 10.1111/j.1365-2907.2010.00182.x; SLATKIN M, 1991, GENETICS, V129, P555; Stamatis C, 2009, J BIOGEOGR, V36, P515, DOI 10.1111/j.1365-2699.2008.02013.x; Taberlet P, 1998, MOL ECOL, V7, P453, DOI 10.1046/j.1365-294x.1998.00289.x; Tamura K, 2011, MOL BIOL EVOL, V28, P2731, DOI 10.1093/molbev/msr121; THOMPSON JD, 1994, NUCLEIC ACIDS RES, V22, P4673, DOI 10.1093/nar/22.22.4673; vansAsch B., 2011, ANIMAL GENETICS, V43, P35; Velickovic N, 2012, RUSS J GENET+, V48, P859, DOI 10.1134/S1022795412080078; Velikovi N., 2010, ARCH BIOL SCI, V62, P807
cited reference count 44
times cited 4
total times cited count (wos, bci, and cscd) 4
publisher WILEY-BLACKWELL
publisher city HOBOKEN
publisher address 111 RIVER ST, HOBOKEN 07030-5774, NJ USA
issn 0305-0270
29-character source abbreviation J BIOGEOGR
iso source abbreviation J. Biogeogr.
publication date APR
year published 2015
volume 42
issue 4
beginning page 716
ending page 728
digital object identifier (doi) 10.1111/jbi.12458
page count 13
web of science category Ecology; Geography, Physical
subject category Environmental Sciences & Ecology; Physical Geography
document delivery number CD2LO
unique article identifier WOS:000350910100011
link http://onlinelibrary.wiley.com/doi/10.1111/jbi.12458/abstract