HOME  »  Members  »  Integrated  »  Post-Docs
 
title Protein profiles of Escherichia coli and Staphylococcus warneri are altered by photosensitization with cationic porphyrins
authors Alves, E; Esteves, AC; Correia, A; Cunha, A; Faustino, MAF; Neves, MGPMS; Almeida, A
author full name Alves, Eliana; Esteves, Ana Cristina; Correia, Antonio; Cunha, Angela; Faustino, Maria A. F.; Neves, Maria G. P. M. S.; Almeida, Adelaide
title Protein profiles of Escherichia coli and Staphylococcus warneri are altered by photosensitization with cationic porphyrins
nationality internacional
source PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
language English
document type Article
keywords plus INTENSIVE-CARE-UNIT; BOVINE SERUM-ALBUMIN; PHOTODYNAMIC INACTIVATION; PORPHYROMONAS-GINGIVALIS; MEMBRANE PHOSPHOLIPIDS; SINGLET OXYGEN; OXIDATION; BACTERIA; THERAPY; CELLS
abstract Oxidative stress induced by photodynamic treatment of microbial cells causes irreversible damages to vital cellular components such as proteins. Photodynamic inactivation (PDI) of bacteria, a promising therapeutic approach for the treatment of superficial and localized skin and oral infections, can be achieved by exciting a photosensitizing agent with visible light in an oxygenated environment. Although some studies have addressed the oxidative alterations of PDI in bacterial proteins, the present study is the first to compare the electrophoretic profiles of proteins of Gram-positive and Gram-negative bacteria, having two structurally different porphyrins, with different kinetics of photoinactivation. The cationic porphyrins 5,10,15-tris(1-methylpyridinium-4-yl)-20-(pentafluorophenyl)porphyrin tri-iodide (Tri-Py+-Me-PF) and 5,10,15,20-tetrakis(1-methylpyridinium-4-yl)porphyrin tetra-iodide (Tetra-Py+-Me) were used to photosensitize Escherichia coli and Staphylococcus warneri upon white light irradiation at an irradiance of 4.0 mW cm(-2). After different photosensitization periods, proteins were extracted from bacteria and analyzed using one-dimensional SDS-PAGE. Apparent molecular weights and band intensities were determined after an irradiation period corresponding to a reduction of 4 log(10) in cell viability. After photodynamic treatment, there was a general loss of bacterial proteins, assigned to large-scale protein degradation. Protein loss was more pronounced after PDI with Tri-Py+-Me-PF in both bacteria. There was also an increase in the concentration of some proteins as well as an increase in the molecular weight of other proteins. We show that proteins of E. coli and S. warneri are important targets of PDI. Although there is an attempt of cellular response to the PDI-induced damage by overexpression of a limited number of proteins, the damage is lethal. Our results show that changes occurring in the protein pattern during photodynamic treatment are different with the two photosensitizers, which helps to explain the different inactivation kinetics of the two bacteria. SDS-PAGE is a rational approach to assign the type of cellular response to stress that is being induced in the cells.
author address [Alves, Eliana; Esteves, Ana Cristina; Correia, Antonio; Cunha, Angela; Almeida, Adelaide] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Alves, Eliana; Esteves, Ana Cristina; Correia, Antonio; Cunha, Angela; Almeida, Adelaide] Univ Aveiro, Ctr Environm & Marine Studies CESAM, P-3810193 Aveiro, Portugal; [Faustino, Maria A. F.; Neves, Maria G. P. M. S.] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal; [Faustino, Maria A. F.; Neves, Maria G. P. M. S.] Univ Aveiro, Organ Chem Res Unit QOPNA, P-3810193 Aveiro, Portugal
reprint address Neves, MGPMS (reprint author), Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal.
e-mail address aalmeida@ua.pt
researcherid number Correia, Antonio/B-1593-2008; Almeida, Adelaide/D-9899-2011; Esteves, Ana Cristina/B-2939-2008; Cunha, Angela/E-9537-2011; Alves, Eliana/K-3158-2013; CESAM, UA/M-3762-2015; Faustino, Maria Amparo/J-5787-2012
orcid number Alves, Eliana/0000-0001-5396-6536; Correia, Antonio/0000-0002-5115-1429; Almeida, Adelaide/0000-0002-8422-8664; Esteves, Ana Cristina/0000-0003-2239-2976; Cunha, Angela/0000-0002-9118-3521; Faustino, Maria Amparo/0000-0003-4423-3802
funding agency and grant number University of Aveiro; Fundacao para a Ciencia e a Tecnologia (FCT, Portugal); European Union; QREN; COMPETE; FEDER [Pest-C/MAR/LA0017/2013, PEst-C/QUI/UI0062/2013, FCOMP-01-0124-FEDER-037296]; FCT [BD/41806/2007, BPD/38008/2007]
funding text The authors are thankful to the University of Aveiro, Fundacao para a Ciencia e a Tecnologia (FCT, Portugal), European Union, QREN, COMPETE and FEDER for funding the Centre for Environmental and Marine Studies (CESAM) unit (project Pest-C/MAR/LA0017/2013) and the QOPNA research unit (project PEst-C/QUI/UI0062/2013, FCOMP-01-0124-FEDER-037296). Eliana Alves (BD/41806/2007) and Ana Cristina Esteves (BPD/38008/2007) are grateful to FCT for their grants.
cited references Almeida A., 2009, Portuguese Patent, Patent No. [PT 103828, 103828]; Alves E, 2011, PLOS ONE, V6, DOI 10.1371/journal.pone.0020970; George S, 2008, PHOTOCHEM PHOTOBIOL, V84, P734, DOI 10.1111/j.1751-1097.2007.00244.x; Bhatti M, 2001, CURR MICROBIOL, V43, P96, DOI 10.1007/s002840010268; Oliveira A, 2009, J APPL MICROBIOL, V106, P1986, DOI 10.1111/j.1365-2672.2009.04168.x; Shacter E, 2000, DRUG METAB REV, V32, P307, DOI 10.1081/DMR-100102336; Davies MJ, 2003, BIOCHEM BIOPH RES CO, V305, P761, DOI 10.1016/S0006-291X(03)00817-9; Segalla A, 2002, PHOTOCH PHOTOBIO SCI, V1, P641, DOI 10.1039/b202031a; Center KJ, 2003, J CLIN MICROBIOL, V41, P4660, DOI 10.1128/JCM.41.10.4660-4665.2003; Fu FF, 2013, J PROTEOME RES, V12, P4478, DOI 10.1021/pr400533m; Tavares A, 2011, PHOTOCH PHOTOBIO SCI, V10, P1659, DOI 10.1039/c1pp05097d; Alves E, 2014, FUTURE MED CHEM, V6, P141, DOI 10.4155/fmc.13.211; Lemos MFL, 2010, PROTEOMICS, V10, P873, DOI 10.1002/pmic.200900470; FOOTE CS, 1991, PHOTOCHEM PHOTOBIOL, V54, P659, DOI 10.1111/j.1751-1097.1991.tb02071.x; Carvalho CMB, 2010, ACS NANO, V4, P7133, DOI 10.1021/nn1026092; Bertoloni G, 2000, BBA-GEN SUBJECTS, V1475, P169, DOI 10.1016/S0304-4165(00)00071-4; Gomes MC, 2013, PHOTOCH PHOTOBIO SCI, V12, P262, DOI 10.1039/c2pp25149c; Gomes MC, 2011, PHOTOCH PHOTOBIO SCI, V10, P1735, DOI 10.1039/c1pp05174a; Agostinis P, 2011, CA-CANCER J CLIN, V61, P250, DOI 10.3322/caac.20114; Valduga G, 1999, BIOCHEM BIOPH RES CO, V256, P84, DOI 10.1006/bbrc.1999.0190; Alves E, 2013, BIOORGAN MED CHEM, V21, P4311, DOI 10.1016/j.bmc.2013.04.065; Davies MJ, 2004, PHOTOCH PHOTOBIO SCI, V3, P17, DOI 10.1039/b307576c; Alves E, 2009, BMC MICROBIOL, V9, DOI 10.1186/1471-2180-9-70; Han MJ, 2006, MICROBIOL MOL BIOL R, V70, P362, DOI 10.1128/MMBR.00036-05; Gracanin M, 2009, FREE RADICAL BIO MED, V47, P92, DOI 10.1016/j.freeradbiomed.2009.04.015; NITZAN Y, 1992, PHOTOCHEM PHOTOBIOL, V55, P89, DOI 10.1111/j.1751-1097.1992.tb04213.x; Dosselli R, 2012, J PROTEOMICS, V77, P329, DOI 10.1016/j.jprot.2012.09.007; Hira V, 2010, J CLIN MICROBIOL, V48, P3876, DOI 10.1128/JCM.00967-10; Alves E, 2013, RAPID COMMUN MASS SP, V27, P2717, DOI 10.1002/rcm.6739; RAETZ CRH, 1978, MICROBIOL REV, V42, P614; Packer S, 2000, LASER MED SCI, V15, P24, DOI 10.1007/s101030050043; Juzeniene A, 2006, J ENVIRON PATHOL TOX, V25, P7; ALTUVIA S, 1994, MOL MICROBIOL, V13, P265, DOI 10.1111/j.1365-2958.1994.tb00421.x; Arrojado C, 2011, PHOTOCH PHOTOBIO SCI, V10, P1691, DOI 10.1039/c1pp05129f; Arslan F, 2011, ANN CLIN MICROB ANTI, V10, DOI 10.1186/1476-0711-10-14; Bhatti M, 1998, PHOTOCHEM PHOTOBIOL, V68, P370, DOI 10.1111/j.1751-1097.1998.tb09694.x; Bolean M, 2010, PHOTOMED LASER SURG, V28, pS79, DOI 10.1089/pho.2009.2635; Booth L, 2002, FREE RADICAL BIO MED, V33, pS390; Bose B, 2006, J PHOTOCH PHOTOBIO B, V85, P49, DOI 10.1016/j.jphotobiol.2006.04.005; Buettner G. R., 2011, PHOTOBIOLOGICAL SCI; Carvalho CMB, 2007, J PHOTOCH PHOTOBIO B, V88, P112, DOI 10.1016/j.jphotobiol.2007.04.015; Cimiotti JP, 2007, INFECT CONT HOSP EP, V28, P326, DOI 10.1086/511998; Costa L, 2014, J VIROL METHODS, V209, P103, DOI 10.1016/j.jviromet.2014.09.013; Costa L, 2011, ANTIVIR RES, V91, P278, DOI 10.1016/j.antiviral.2011.06.007; Jori G., 2011, PHOTODYNAMIC INACTIV, P3, DOI DOI 10.1039/9781849733083-00001; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Lemos MFL, 2010, CHEMOSPHERE, V79, P570, DOI 10.1016/j.chemosphere.2010.01.055; Lin SL, 2012, PHOTOCHEM PHOTOBIOL, V88, P985, DOI 10.1111/j.1751-1097.2012.01154.x; Alves E, 2013, RAPID COMMUN MASS SP, V27, P1607, DOI 10.1002/rcm.6614; NEUHOFF V, 1985, ELECTROPHORESIS, V6, P427, DOI 10.1002/elps.1150060905; Ouedraogo GD, 2003, PHOTOCHEM PHOTOBIOL, V77, P192, DOI 10.1562/0031-8655(2003)077<0192:SROSET>2.0.CO;2; Pattison DI, 2012, PHOTOCH PHOTOBIO SCI, V11, P38, DOI 10.1039/c1pp05164d; Silhavy TJ, 2010, CSH PERSPECT BIOL, V2, DOI 10.1101/cshperspect.a000414; Silvester JA, 1998, ARCH BIOCHEM BIOPHYS, V350, P249, DOI 10.1006/abbi.1997.0495; St Denis TG, 2011, PHOTOCHEM PHOTOBIOL, V87, P707, DOI 10.1111/j.1751-1097.2011.00902.x; Stöllberger Claudia, 2006, J Infect, V52, pe15, DOI 10.1016/j.jinf.2005.04.016; Tamarit J, 1998, J BIOL CHEM, V273, P3027, DOI 10.1074/jbc.273.5.3027
cited reference count 57
publisher ROYAL SOC CHEMISTRY
publisher city CAMBRIDGE
publisher address THOMAS GRAHAM HOUSE, SCIENCE PARK, MILTON RD, CAMBRIDGE CB4 0WF, CAMBS, ENGLAND
issn 1474-905X
29-character source abbreviation PHOTOCH PHOTOBIO SCI
iso source abbreviation Photochem. Photobiol. Sci.
year published 2015
volume 14
issue 6
beginning page 1169
ending page 1178
digital object identifier (doi) 10.1039/c4pp00194j
page count 10
web of science category Biochemistry & Molecular Biology; Biophysics; Chemistry, Physical
subject category Biochemistry & Molecular Biology; Biophysics; Chemistry
document delivery number CJ8FD
unique article identifier WOS:000355734700011