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title Secretome analysis of Trichoderma atroviride T17 biocontrol of Guignardia citricarpa
authors de Lima, FB; Felix, C; Osorio, N; Alves, A; Vitorino, R; Domingues, P; Correia, A; Ribeiro, RTD; Esteves, AC
author full name de Lima, Fernanda Blauth; Felix, Carina; Osorio, Nadia; Alves, Artur; Vitorino, Rui; Domingues, Pedro; Correia, Antonio; da Silva Ribeiro, Rute T.; Esteves, Ana Cristina
title Secretome analysis of Trichoderma atroviride T17 biocontrol of Guignardia citricarpa
nationality internacional
source BIOLOGICAL CONTROL
language English
document type Article
author keywords Trichoderma atroviride; Guignardia citricarpa; Biological control; Proteomics; Pathogen antagonism
keywords plus CITRUS BLACK SPOT; FUNGAL PLANT-PATHOGENS; IN-VITRO ANTAGONISM; RHIZOCTONIA-SOLANI; CELL-WALL; SCLEROTIUM-ROLFSII; MOLECULAR CHARACTERIZATION; BOTRYTIS-CINEREA; GLOMUS-MOSSEAE; ENCODING GENE
abstract The fungal species Guignardia citricarpa is an important pathogen in citriculture. Members of the fungal genus Trichoderma are recognized as biocontrol agents but studies on the interactions between both fungi are scarce. This study aimed to identify extracellular proteins secreted by Trichoderma atroviride T17 that are related to the control of G. citricarpa. Two-dimensional gel electrophoresis (2D) was used to study the patterns of proteins secreted by T. atroviride T17 in medium containing glucose (control) and in medium containing G. citricarpa GC3 inactivated mycelium. We identified 59 of the 116 spots differentially expressed (50.86%) by LC-MS/MS. Of these, we highlight the presence of glycoside hydrolases (CAZy families 3, 43, 54, 76 and 93), chitinase, mutanase, alpha-1,3-glucanase, alpha-1,2-mannosidase, carboxylic hydrolase ester, carbohydrate-binding module family 13, glucan 1,3-beta-glucosidase, alpha-galactosidase and Neutral protease 2. These proteins are related to mycoparasitism processes, stimuli and therefore to the biological control of pathogens. The results obtained are in agreement with reports describing an increase in the secretion of proteins related to mycoparasitism and biological control and a reduction in the secretion of proteins related to the metabolism of Trichoderma species grown in the presence of the pathogen. Moreover, these results are pioneer in understanding T. atroviride interaction with G. citricarpa. For the first time, we identified potential candidate proteins that may have a role in the antagonism mechanism of G. citricarpa by T. atroviride T17. Thus our results shed a light into the molecular mechanisms that T. atroviride use to control G. citricarpa. (C) 2016 Elsevier Inc. All rights reserved.
author address [de Lima, Fernanda Blauth; da Silva Ribeiro, Rute T.] Univ Caxias Sul, Dept Biotechnol, Vila Seca, RS, Brazil; [Felix, Carina; Osorio, Nadia; Alves, Artur; Correia, Antonio; Esteves, Ana Cristina] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Felix, Carina; Osorio, Nadia; Alves, Artur; Correia, Antonio; Esteves, Ana Cristina] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Vitorino, Rui] Univ Aveiro, Inst Biomed iBiMED, Dept Med Sci, P-3810193 Aveiro, Portugal; [Vitorino, Rui] Univ Porto, Dept Physiol & Cardiothorac Surg, Fac Med, Rua Campo Alegre 823, P-4100 Oporto, Portugal; [Domingues, Pedro] Univ Aveiro, Dept Chem, P-3810193 Aveiro, Portugal; [Domingues, Pedro] Univ Aveiro, QOPNA, P-3810193 Aveiro, Portugal
reprint address de Lima, FB (reprint author), Univ Caxias Sul, Dept Biotechnol, St Francisco Getulio Vargas 1130, BR-95070560 Caxias Do Sul, RS, Brazil.
e-mail address nandalima85@gmail.com
funding agency and grant number FEDER funding through COMPETE program; national funding through FCT within research project ALIEN [PTDC/AGR-PRO/2183/2014, UID/AMB/50017/2013, UID/BIM/04501/2013, UID/IC/00051/2013, PEst-C/QUI/UI0062/2013]; RNEM (Portuguess Mass Spectrometry Network); FCT [IF/00835/2013, BPD/102572/2014, BD/97613/2013]; CAPES; FAPERGS
funding text This study was partially supported by FEDER funding through COMPETE program and by national funding through FCT within the research project ALIEN (PTDC/AGR-PRO/2183/2014) and to CESAM (UID/AMB/50017/2013), ibiMED (UID/BIM/04501/2013), UniC (UID/IC/00051/2013), QOPNA (PEst-C/QUI/UI0062/2013) and RNEM (Portuguess Mass Spectrometry Network). The authors acknowledge FCT financing to A Alves (IF/00835/2013), AC Esteves (BPD/102572/2014) and C Felix (BD/97613/2013). F Lima acknowledges CAPES for the PhD scholarship to develop part of the research project: Proteomic analysis of T. atroviride and Trichoderma harzianum against Guignardia citricarpa, pathogenic agent of Black spot of Citrus at the University of Aveiro and FAPERGS for the PhD scholarship in Biotechnology at the University of Caxias do Sul (Brazil).
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cited reference count 96
publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
publisher city SAN DIEGO
publisher address 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
issn 1049-9644
29-character source abbreviation BIOL CONTROL
iso source abbreviation Biol. Control
publication date AUG
year published 2016
volume 99
beginning page 38
ending page 46
digital object identifier (doi) 10.1016/j.biocontrol.2016.04.009
page count 9
web of science category Biotechnology & Applied Microbiology; Entomology
subject category Biotechnology & Applied Microbiology; Entomology
document delivery number DO2IC
unique article identifier WOS:000377602200006
link http://authors.elsevier.com/sd/article/S1049964416300597