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  • Authors Viegas, I; Araujo, PM; Rocha, AD; Villegas, A; Jones, JG; Ramos, JA; Masero, JA; Alves, JA
  • Author Full Name Viegas, Ivan; Araujo, Pedro M.; Rocha, Afonso D.; Villegas, Auxiliadora; Jones, John G.; Ramos, Jaime A.; Masero, Jose A.; Alves, Jose A.
  • Title Metabolic plasticity for subcutaneous fat accumulation in a longdistance migratory bird traced by (H2O)-H-2
  • Nationality internacional
  • Source JOURNAL OF EXPERIMENTAL BIOLOGY
  • Language English
  • Document Type Article
  • Author Keywords NMR; Deuterated water; Lipogenesis; Migration; Lipid accumulation; Godwits
  • Keywords Plus TAILED GODWITS LIMOSA; DISTANCE MIGRANT SHOREBIRD; DE-NOVO LIPOGENESIS; ACID-COMPOSITION; ADIPOSE-TISSUE; SELECTIVE MOBILIZATION; ZONOTRICHIA-ALBICOLLIS; EXERCISE PERFORMANCE; WESTERN SANDPIPERS; DIET PREFERENCES
  • Abstract The migrant black- tailed godwit (Limosa limosa) traditionally used natural wetlands in the Iberian Peninsula to prepare for migratory flights by feeding mainly in estuaries. In recent decades, this species has become increasingly dependent on rice fields, thereby relying on a plant-based diet for fuelling. Dietary fatty acids (FA) seem to be determinant to the composition of accumulated subcutaneous fat in migratory birds. It is still unclear whether metabolic plasticity allows for modification and/ or synthesis of FA, contributing to a lipid profile that enables a successful migratory performance. Deuterated water was administered to captive black-tailed godwits submitted to two diets (fly larvae versus rice) and the incorporation of deuterium (H-2) into subcutaneous triglycerides was analyzed by NMR. A recently developed localized biopsy method for sampling subcutaneous fat was employed with later successful release of all birds into the wild. The average chemical structure reflected mostly a mixture of saturated and monounsaturated 16- and 18- carbon FA, a profile frequently found in migrant birds. Significantly higher levels of polyunsaturated FA, as well as detectable levels of n-3 FA, were observed in fly-larvae-fed birds. Excess H-2-enrichments in FA revealed significantly higher rates of fractional de novo lipogenesis and FA desaturation capacity in rice-fed birds. This novel and nonlethal tracer method revealed the capacity of this species to alter its lipid metabolism to compensate for a poorer dietary lipid contribution. Because of its versatility, adapting this method to other scenarios and/ or other migratory species is considered feasible and costeffective.
  • Author Address [Viegas, Ivan; Jones, John G.] Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal; [Viegas, Ivan] Univ Coimbra, Dept Life Sci, CFE, P-3000456 Coimbra, Portugal; [Araujo, Pedro M.; Rocha, Afonso D.; Ramos, Jaime A.] Univ Coimbra, Dept Life Sci, MARE Marine & Environm Sci Ctr, P-3000456 Coimbra, Portugal; [Villegas, Auxiliadora; Masero, Jose A.] Univ Extremadura, Conservat Biol Res Grp, Area Zool, Ave Elvas S-N, E-06071 Badajoz, Spain; [Alves, Jose A.] Univ Aveiro, Dept Biol, CESAM, Campus Univ Santiago, P-3180193 Aveiro, Portugal; [Alves, Jose A.] Univ Iceland, South Iceland Res Ctr, IS-800 Bankavegur, Selfoss, Iceland
  • Reprint Address Viegas, I (reprint author), Univ Coimbra, Ctr Neurosci & Cell Biol, P-3004517 Coimbra, Portugal.; Viegas, I (reprint author), Univ Coimbra, Dept Life Sci, CFE, P-3000456 Coimbra, Portugal.
  • E-mail Address iviegas@ci.uc.pt
  • ResearcherID Number Jones, John/A-8684-2010; Viegas, Ivan/F-4527-2011; Masero, Jose A./A-9478-2008; Ramos, Jaime/B-6616-2018
  • ORCID Number Jones, John/0000-0002-3745-3885; Viegas, Ivan/0000-0003-2589-2212; Masero, Jose A./0000-0001-5318-4833; Ramos, Jaime/0000-0002-9533-987X; Alves, Jose/0000-0001-7182-0936
  • Funding Agency and Grant Number Fundacao para a Ciencia e Tecnologia (FCT) [SFRH/BPD/90032/2012, SFRH/BD/69238/2010, SFRH/BD/74228/2010, SFRH/BPD/91527/2012, EXCL/DTP-PIC/0069/2012]; FEDER; FCT [RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012]; Rede Nacional de Ressonancia Magnetica Nuclear (RNRMN)
  • Funding Text The authors acknowledge financial support from Fundacao para a Ciencia e Tecnologia (FCT) in the form of fellowships to I.V. (SFRH/BPD/90032/2012), P.M.A. (SFRH/BD/69238/2010), A.D.R. (SFRH/BD/74228/2010) and J.A.A. (SFRH/BPD/91527/2012) and research project EXCL/DTP-PIC/0069/2012. NMR data were collected at the UC-NMR facility that is supported by FEDER and FCT (RECI/QEQ-QFI/0168/2012, CENTRO-07-CT62-FEDER-002012) and Rede Nacional de Ressonancia Magnetica Nuclear (RNRMN).
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  • Cited Reference Count 50
  • Publisher COMPANY OF BIOLOGISTS LTD
  • Publisher City CAMBRIDGE
  • Publisher Address BIDDER BUILDING CAMBRIDGE COMMERCIAL PARK COWLEY RD, CAMBRIDGE CB4 4DL, CAMBS, ENGLAND
  • ISSN 0022-0949
  • Character Source Abbreviation J EXP BIOL
  • ISO Source Abbreviation J. Exp. Biol.
  • Publication Date MAR 15
  • Year Published 2017
  • Volume 220
  • Issue 6
  • Beginning Page 1072
  • Ending Page 1078
  • Digital Object Identifier (DOI) 10.1242/jeb.150490
  • Subject Category 7
  • Document Delivery Number Biology
  • Unique Article Identifier Life Sciences & Biomedicine - Other Topics
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