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title Investigating heritability of cadmium tolerance in Chironomus riparius natural populations: A physiological approach
authors Pedrosa, J; Gravato, C; Campos, D; Cardoso, P; Figueira, E; Nowak, C; Soares, AMVM; Barata, C; Pestana, JLT
author full name Pedrosa, Joao; Gravato, Carlos; Campos, Diana; Cardoso, Paulo; Figueira, Etelvina; Nowak, Carsten; Soares, Amadeu M. V. M.; Barata, Carlos; Pestana, Joao L. T.
title Investigating heritability of cadmium tolerance in Chironomus riparius natural populations: A physiological approach
nationality internacional
source CHEMOSPHERE
language English
document type Article
author keywords Metal tolerance; Metallothioneins; Glutathione; Oxidative damage; Energy allocation; Protein profile
keywords plus ANTIOXIDANT ENZYME-ACTIVITIES; GLUTATHIONE-S-TRANSFERASE; DRIVEN GENETIC EROSION; OXIDATIVE STRESS; DAPHNIA-MAGNA; PHENOTYPIC PLASTICITY; ENVIRONMENTAL-POLLUTANTS; LIPID-PEROXIDATION; AQUATIC ORGANISMS; CLIMATE-CHANGE
abstract Physiological responses allow populations to cope with metal contamination and can be involved in the evolution of tolerance under historical metal contamination scenarios. Here we investigate physiological aspects that might be underlying the heritable high tolerance to cadmium (Cd) in two Chironomus riparius populations collected from historically metal contaminated sites in comparison to two populations from reference sites. To evaluate differences in the physiological response to short-term Cd exposure, protein expression profiles, metallothioneins [MTs] and several antioxidant defences such as total glutathione (GSH(t)), catalase (CAT) and glutathione-S-transferases [GSTs], were measured in all four populations reared for at least 8 generations under laboratory clean conditions. Cd-induced oxidative damage in lipids and energy related parameters (energy consumption and energy reserves) were also assessed. Results showed two major gradients of protein profiles according to Cd concentration and population tolerance. Furthermore, Cd-tolerant populations showed higher baseline levels of MTs and GSHt while Cd-sensitive populations, collected from reference sites, showed significant induction of GSHt levels with Cd exposure that were nonetheless insufficient to avoid increased oxidative damage to lipids. Cd exposure had no clear effects on the antioxidant enzymes or energy reserves but triggered a general increase in energy consumption. Finally, energy consumption was higher in Cd-tolerant populations across experimental conditions. Altogether, results demonstrate that inherited Cd-tolerance in these midge populations is related, at least in part, with different constitutive levels and plasticity of different defence mechanisms confirming the validity of using multiple physiological traits when studying evolution of tolerance. (C) 2016 Elsevier Ltd. All rights reserved.
author address [Pedrosa, Joao; Gravato, Carlos; Campos, Diana; Cardoso, Paulo; Figueira, Etelvina; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Pedrosa, Joao; Gravato, Carlos; Campos, Diana; Cardoso, Paulo; Figueira, Etelvina; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Pedrosa, Joao; Nowak, Carsten] Conservat Genet Grp, Senckenberg Res Inst, Clamecystr 12, D-63571 Gelnhausen, Germany; [Pedrosa, Joao; Nowak, Carsten] Nat Hist Museum Frankfurt, Clamecystr 12, D-63571 Gelnhausen, Germany; [Barata, Carlos] IDAEA CSIC, Dept Environm Chem, Jordi Girona 18-26, Barcelona 08034, Spain
reprint address Pedrosa, J; Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal.; Pedrosa, J; Pestana, JLT (reprint author), Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal.
e-mail address jpedrosa@ua.pt; jpestana@ua.pt
orcid number Cardoso, Paulo/0000-0002-1234-5861; Figueira, Etelvina/0000-0002-6763-2665
funding agency and grant number Portuguese Science Foundation (FCT) through CESAM [UID/AMB/50017/2013]; COMPETE program (Programa Operacional Fatores de Competitividade, FEDER component); National funding through FCT-Fundacao para a Ciencia e Tecnologia within the research project MIDGE Microevolutionary Dynamics and Genetic Erosion in pollution-affected Chironomus [PTDC/BIA-BEC/104125/2008]; FCT; POPH/FSE (Programa Operacional Potential Humano/Fundo Social Europeu) [SFRH/BPD/103897/2014]; Joao Pedrosa PhD grant [SFRH/BD/75606/2010]; Diana Campos PhD grant [SFRH/BD/87370/2012]; Paulo Cardoso PhD grant [SFRH/BD/101140/2014]; [IF/01401/2014]
funding text This work was supported by the Portuguese Science Foundation (FCT) through CESAM: UID/AMB/50017/2013. We are thankful for the financial support of COMPETE program (Programa Operacional Fatores de Competitividade, FEDER component) and by National funding through FCT-Fundacao para a Ciencia e Tecnologia within the research project MIDGE Microevolutionary Dynamics and Genetic Erosion in pollution-affected Chironomus (Ref: PTDC/BIA-BEC/104125/2008). We are also thankful to FCT and POPH/FSE (Programa Operacional Potential Humano/Fundo Social Europeu) for the post-doctoral fellowships of Joao L.T. Pestana (SFRH/BPD/103897/2014), Joao Pedrosa PhD grant (SFRH/BD/75606/2010), Diana Campos PhD grant (SFRH/BD/87370/2012), Paulo Cardoso PhD grant (SFRH/BD/101140/2014) and for the contract of Carlos Gravato (IF/01401/2014).
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cited reference count 107
publisher PERGAMON-ELSEVIER SCIENCE LTD
publisher city OXFORD
publisher address THE BOULEVARD, LANGFORD LANE, KIDLINGTON, OXFORD OX5 1GB, ENGLAND
issn 0045-6535
29-character source abbreviation CHEMOSPHERE
iso source abbreviation Chemosphere
publication date MAR
year published 2017
volume 170
beginning page 83
ending page 94
digital object identifier (doi) 10.1016/j.chemosphere.2016.12.008
page count 12
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number EJ0KK
unique article identifier WOS:000392897100011