HOME  »  Members  »  Integrated  »  Post-Docs
title Investigating heritability of cadmium tolerance in Chironomus riparius natural populations: A physiological approach
authors Pedrosa, J; Gravato, C; Campos, D; Cardoso, P; Figueira, E; Nowak, C; Soares, AMVM; Barata, C; Pestana, JLT
author full name Pedrosa, Joao; Gravato, Carlos; Campos, Diana; Cardoso, Paulo; Figueira, Etelvina; Nowak, Carsten; Soares, Amadeu M. V. M.; Barata, Carlos; Pestana, Joao L. T.
title Investigating heritability of cadmium tolerance in Chironomus riparius natural populations: A physiological approach
nationality internacional
language English
document type Article
author keywords Metal tolerance; Metallothioneins; Glutathione; Oxidative damage; Energy allocation; Protein profile
abstract Physiological responses allow populations to cope with metal contamination and can be involved in the evolution of tolerance under historical metal contamination scenarios. Here we investigate physiological aspects that might be underlying the heritable high tolerance to cadmium (Cd) in two Chironomus riparius populations collected from historically metal contaminated sites in comparison to two populations from reference sites. To evaluate differences in the physiological response to short-term Cd exposure, protein expression profiles, metallothioneins [MTs] and several antioxidant defences such as total glutathione (GSH(t)), catalase (CAT) and glutathione-S-transferases [GSTs], were measured in all four populations reared for at least 8 generations under laboratory clean conditions. Cd-induced oxidative damage in lipids and energy related parameters (energy consumption and energy reserves) were also assessed. Results showed two major gradients of protein profiles according to Cd concentration and population tolerance. Furthermore, Cd-tolerant populations showed higher baseline levels of MTs and GSHt while Cd-sensitive populations, collected from reference sites, showed significant induction of GSHt levels with Cd exposure that were nonetheless insufficient to avoid increased oxidative damage to lipids. Cd exposure had no clear effects on the antioxidant enzymes or energy reserves but triggered a general increase in energy consumption. Finally, energy consumption was higher in Cd-tolerant populations across experimental conditions. Altogether, results demonstrate that inherited Cd-tolerance in these midge populations is related, at least in part, with different constitutive levels and plasticity of different defence mechanisms confirming the validity of using multiple physiological traits when studying evolution of tolerance. (C) 2016 Elsevier Ltd. All rights reserved.
author address [Pedrosa, Joao; Gravato, Carlos; Campos, Diana; Cardoso, Paulo; Figueira, Etelvina; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Pedrosa, Joao; Gravato, Carlos; Campos, Diana; Cardoso, Paulo; Figueira, Etelvina; Soares, Amadeu M. V. M.; Pestana, Joao L. T.] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Pedrosa, Joao; Nowak, Carsten] Conservat Genet Grp, Senckenberg Res Inst, Clamecystr 12, D-63571 Gelnhausen, Germany; [Pedrosa, Joao; Nowak, Carsten] Nat Hist Museum Frankfurt, Clamecystr 12, D-63571 Gelnhausen, Germany; [Barata, Carlos] IDAEA CSIC, Dept Environm Chem, Jordi Girona 18-26, Barcelona 08034, Spain
reprint address Pedrosa, J; Pestana, JLT (reprint author), Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal.; Pedrosa, J; Pestana, JLT (reprint author), Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal.
e-mail address jpedrosa@ua.pt; jpestana@ua.pt
orcid number Cardoso, Paulo/0000-0002-1234-5861; Figueira, Etelvina/0000-0002-6763-2665
funding agency and grant number Portuguese Science Foundation (FCT) through CESAM [UID/AMB/50017/2013]; COMPETE program (Programa Operacional Fatores de Competitividade, FEDER component); National funding through FCT-Fundacao para a Ciencia e Tecnologia within the research project MIDGE Microevolutionary Dynamics and Genetic Erosion in pollution-affected Chironomus [PTDC/BIA-BEC/104125/2008]; FCT; POPH/FSE (Programa Operacional Potential Humano/Fundo Social Europeu) [SFRH/BPD/103897/2014]; Joao Pedrosa PhD grant [SFRH/BD/75606/2010]; Diana Campos PhD grant [SFRH/BD/87370/2012]; Paulo Cardoso PhD grant [SFRH/BD/101140/2014]; [IF/01401/2014]
funding text This work was supported by the Portuguese Science Foundation (FCT) through CESAM: UID/AMB/50017/2013. We are thankful for the financial support of COMPETE program (Programa Operacional Fatores de Competitividade, FEDER component) and by National funding through FCT-Fundacao para a Ciencia e Tecnologia within the research project MIDGE Microevolutionary Dynamics and Genetic Erosion in pollution-affected Chironomus (Ref: PTDC/BIA-BEC/104125/2008). We are also thankful to FCT and POPH/FSE (Programa Operacional Potential Humano/Fundo Social Europeu) for the post-doctoral fellowships of Joao L.T. Pestana (SFRH/BPD/103897/2014), Joao Pedrosa PhD grant (SFRH/BD/75606/2010), Diana Campos PhD grant (SFRH/BD/87370/2012), Paulo Cardoso PhD grant (SFRH/BD/101140/2014) and for the contract of Carlos Gravato (IF/01401/2014).
cited references Agra AR, 2011, ECOTOXICOLOGY, V20, P552, DOI 10.1007/s10646-011-0621-5; Amiard JC, 2006, AQUAT TOXICOL, V76, P160, DOI 10.1016/j.aquatox.2005.08.015; Anderson MJ, 2008, PERMANOVA PRIMER GUI; ASTM, 1980, MAT; BAKER MA, 1990, ANAL BIOCHEM, V190, P360, DOI 10.1016/0003-2697(90)90208-Q; Barata C, 2000, ENVIRON TOXICOL CHEM, V19, P2314, DOI 10.1897/1551-5028(2000)019<2314:DGRACF>2.3.CO;2; Barata C, 2005, COMP BIOCHEM PHYS C, V140, P175, DOI 10.1016/j.cca.2005.01.013; Bednarska AJ, 2013, ECOTOXICOLOGY, V22, P118, DOI 10.1007/s10646-012-1008-y; Bertin G, 2006, BIOCHIMIE, V88, P1549, DOI 10.1016/j.biochi.2006.10.001; BIRD RP, 1984, METHOD ENZYMOL, V105, P299; Bonduriansky R, 2008, ANNU REV ECOL EVOL S, V40, P103; BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1006/abio.1976.9999; Brandao F, 2015, METALLOMICS, V7, P1352, DOI [10.1039/c5mt00090d, 10.1039/C5MT00090D]; Campos Diana, 2016, Chemosphere, V149, P177, DOI 10.1016/j.chemosphere.2016.01.098; Canesi L, 1999, AQUAT TOXICOL, V46, P67, DOI 10.1016/S0166-445X(98)00116-7; Chandurvelan R, 2013, AQUAT TOXICOL, V140, P303, DOI 10.1016/j.aquatox.2013.06.015; Boudet LC, 2013, ECOTOX ENVIRON SAFE, V89, P52, DOI 10.1016/j.ecoenv.2012.11.008; Choi JH, 2001, COMP BIOCHEM PHYS C, V130, P11, DOI 10.1016/S1532-0456(01)00206-X; Claiborne A., 1985, CRC HDB METHODS OXYG, V1, P283; Connon R, 2008, ENVIRON SCI TECHNOL, V42, P2181, DOI 10.1021/es702469b; Corticeiro S, 2013, BIOMETALS, V26, P879, DOI 10.1007/s10534-013-9664-9; Costa D, 2012, ECOTOXICOLOGY, V21, P2078, DOI 10.1007/s10646-012-0961-9; Dallinger R, 2013, ECOTOXICOLOGY, V22, P767, DOI 10.1007/s10646-013-1071-z; Day T, 2011, AM NAT, V178, pE18, DOI 10.1086/660911; De Coen W. M., 1997, Journal of Aquatic Ecosystem Stress and Recovery, V6, P43, DOI 10.1023/A:1008228517955; De Coen WM, 2003, ENVIRON TOXICOL CHEM, V22, P1632, DOI 10.1897/1551-5028(2003)22<1632:TMBLRB>2.0.CO;2; De Haas EM, 2006, J ANIM ECOL, V75, P148, DOI 10.1111/j.1365-2656.2005.01030.x; Fabrik I, 2008, SENSORS-BASEL, V8, P4081, DOI 10.3390/s8074081; Fisker KV, 2013, COMP BIOCHEM PHYS C, V157, P220, DOI 10.1016/j.cbpc.2012.11.007; Geret F, 2002, BIOMARKERS, V7, P242, DOI 10.1080/13547500210125040; Ghalambor CK, 2007, FUNCT ECOL, V21, P394, DOI 10.1111/j.1365-2435.2007.01283.x; Gienapp P, 2008, MOL ECOL, V17, P167, DOI 10.1111/j.1365-294X.2007.03413.x; Gillis PL, 2014, ECOTOX ENVIRON SAFE, V102, P62, DOI 10.1016/j.ecoenv.2013.12.026; Goncalves SF, 2016, SCI TOTAL ENVIRON, V568, P1054, DOI 10.1016/j.scitotenv.2016.06.149; GRANT A, 1989, MAR POLLUT BULL, V20, P235, DOI 10.1016/0025-326X(89)90438-4; Gravato C, 2006, CHEMOSPHERE, V65, P1821, DOI 10.1016/jh.chemosphere.2006.04.005; Guilherme S, 2008, MAR POLLUT BULL, V56, P845, DOI 10.1016/j.marpolbul.2008.02.003; Haap T, 2016, AQUAT TOXICOL, V170, P112, DOI 10.1016/j.aquatox.2015.11.008; HABIG WH, 1974, J BIOL CHEM, V249, P7130; Harper FA, 1997, FUNCT ECOL, V11, P764, DOI 10.1046/j.1365-2435.1997.00155.x; Hochachka PW, 2001, COMP BIOCHEM PHYS B, V130, P435, DOI 10.1016/S1096-4959(01)00408-0; Isani Gloria, 2014, Biomolecules, V4, P435, DOI 10.3390/biom4020435; Janssens TKS, 2009, INSECT SCI, V16, P3, DOI 10.1111/j.1744-7917.2009.00249.x; Khan FR, 2011, AQUAT TOXICOL, V102, P95, DOI 10.1016/j.aquatox.2011.01.001; Klaassen CD, 1999, ANNU REV PHARMACOL, V39, P267, DOI 10.1146/annurev.pharmtox.39.1.267; Kumari MVR, 1998, FREE RADICAL RES, V29, P93, DOI 10.1080/10715769800300111; LAEMMLI UK, 1970, NATURE, V227, P680, DOI 10.1038/227680a0; Lagisz M, 2005, ARCH ENVIRON CON TOX, V48, P484, DOI 10.1007/s00244-004-0023-2; Latta LC, 2007, BMC EVOL BIOL, V7, DOI 10.1186/1471-2148-7-21; Lee SE, 2006, PROTEOMICS, V6, P945, DOI 10.1002/pmic.200401349; Lee SM, 2006, CHEMOSPHERE, V65, P1074, DOI 10.1016/j.chemosphere.2006.02.042; Leinio S, 2005, COMP BIOCHEM PHYS C, V140, P408, DOI 10.1016/j.cca.2005.04.005; Leitao J, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0068702; Li RJ, 2014, ENVIRON TOXICOL, V29, P298, DOI 10.1002/tox.21758; Livingstone DR, 2003, REV MED VET-TOULOUSE, V154, P427; Livingstone DR, 2001, MAR POLLUT BULL, V42, P656, DOI 10.1016/S0025-326X(01)00060-1; Lopes ML, 2014, MAR POLLUT BULL, V87, P117, DOI 10.1016/j.marpolbul.2014.08.004; Lushchak VI, 2011, AQUAT TOXICOL, V101, P13, DOI 10.1016/j.aquatox.2010.10.006; Machado AAD, 2014, ECOTOXICOLOGY, V23, P1345, DOI 10.1007/s10646-014-1277-8; Marie V, 2006, BIOMETALS, V19, P399, DOI 10.1007/s10534-005-4064-4; Matranga V, 2012, MAR ENVIRON RES, V76, P122, DOI 10.1016/j.marenvres.2011.09.008; Merila J, 2014, EVOL APPL, V7, P1, DOI 10.1111/eva.12137; Meyer JN, 2003, AQUAT TOXICOL, V65, P377, DOI 10.1016/j.aquatox.2003.06.001; MORON MS, 1979, BIOCHIM BIOPHYS ACTA, V582, P67, DOI 10.1016/0304-4165(79)90289-7; Novais SC, 2011, ENVIRON POLLUT, V159, P1836, DOI 10.1016/j.envpol.2011.03.031; OECD, 2011, AC IMM TEST; Oexle S, 2016, J EVOLUTION BIOL, V29, P1328, DOI 10.1111/jeb.12873; Pain-Devin S, 2014, AQUAT TOXICOL, V155, P52, DOI 10.1016/j.aquatox.2014.06.008; Pedrosa J., EVOLUTIONARY C UNPUB; Pereira ME, 1998, WATER SCI TECHNOL, V37, P125, DOI 10.1016/S0273-1223(98)00191-7; Pestana JLT, 2016, ENVIRON RES, V151, P663, DOI 10.1016/j.envres.2016.08.037; Pigliucci M, 2005, TRENDS ECOL EVOL, V20, P481, DOI 10.1016/j.tree.2005.06.001; Planello R, 2010, SCI TOTAL ENVIRON, V408, P1598, DOI 10.1016/j.scitotenv.2010.01.004; Planello R, 2015, ENVIRON TOXICOL, V30, P383, DOI 10.1002/tox.21893; Pook C, 2009, MAR POLLUT BULL, V58, P1063, DOI 10.1016/j.marpolbul.2009.02.003; Postma JF, 1996, ENVIRON TOXICOL CHEM, V15, P332, DOI 10.1897/1551-5028(1996)015<0332:ICEIMA>2.3.CO;2; Ribeiro R, 2013, ECOTOXICOLOGY, V22, P889, DOI 10.1007/s10646-013-1070-0; Ribeiro R, 2012, ENVIRON TOXICOL CHEM, V31, P977, DOI 10.1002/etc.1802; Rodrigues ACM, 2016, AQUAT TOXICOL, V170, P371, DOI 10.1016/j.aquatox.2015.10.018; Rodrigues ACM, 2015, ENVIRON POLLUT, V207, P273, DOI 10.1016/j.envpol.2015.09.035; Rodrigues ACM, 2015, SCI TOTAL ENVIRON, V508, P506, DOI 10.1016/j.scitotenv.2014.12.021; Rowe CL, 2001, COMP BIOCHEM PHYS C, V129, P275, DOI 10.1016/S1532-0456(01)00204-6; Sandbichler AM, 2016, INT J MOL SCI, V17, DOI 10.3390/ijms17010139; Schlichting CD, 2014, EVOLUTION, V68, P656, DOI 10.1111/evo.12348; Shevchenko A, 2006, NAT PROTOC, V1, P2856, DOI 10.1038/nprot.2006.468; Shirley MDF, 1999, EVOLUTION, V53, P826, DOI 10.2307/2640722; SIBLY RM, 1989, BIOL J LINN SOC, V37, P101; Soares HMVM, 1999, ENVIRON POLLUT, V105, P311, DOI 10.1016/S0269-7491(99)00048-2; Sokolova IM, 2012, MAR ENVIRON RES, V79, P1, DOI 10.1016/j.marenvres.2012.04.003; Sornom P, 2012, PLOS ONE, V7, DOI 10.1371/journal.pone.0042435; Stoks R, 2014, EVOL APPL, V7, P42, DOI 10.1111/eva.12108; Sturmbauer C, 1999, MOL BIOL EVOL, V16, P967; Thompson EL, 2012, ENVIRON POLLUT, V170, P102, DOI 10.1016/j.envpol.2012.06.015; TIETZE F, 1969, ANAL BIOCHEM, V27, P502, DOI 10.1016/0003-2697(69)90064-5; Tousova Z, 2016, ENVIRON SCI POLLUT R, V23, P10504, DOI 10.1007/s11356-016-6362-5; Uusi-Heikkila S, 2015, EVOL APPL, V8, P597, DOI 10.1111/eva.12268; Valavanidis A, 2006, ECOTOX ENVIRON SAFE, V64, P178, DOI 10.1016/j.econenv.2005.03.013; Velez C, 2016, CHEMOSPHERE, V156, P128, DOI 10.1016/j.chemosphere.2016.04.067; Vellinger C, 2013, AQUAT TOXICOL, V140, P106, DOI 10.1016/j.aquatox.2013.05.010; Wang JM, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0052954; Wang L, 2011, FOOD CHEM TOXICOL, V49, P3407, DOI 10.1016/j.fct.2011.06.015; Wang MH, 2010, ECOTOXICOLOGY, V19, P273, DOI 10.1007/s10646-009-0410-6; Weng NY, 2014, AQUAT TOXICOL, V146, P61, DOI 10.1016/j.aquatox.2013.10.036; WILLIAMS KA, 1986, OECOLOGIA, V70, P362, DOI 10.1007/BF00379498; Wu H, 2013, ENVIRON SCI POLLUT R, V20, P4085, DOI 10.1007/s11356-012-1362-6; Wu JP, 2012, ARCH ENVIRON CON TOX, V63, P220, DOI 10.1007/s00244-012-9764-5; Xie L, 2009, ENVIRON SCI TECHNOL, V43, P934, DOI 10.1021/es802323r
cited reference count 107
publisher city OXFORD
issn 0045-6535
29-character source abbreviation CHEMOSPHERE
iso source abbreviation Chemosphere
publication date MAR
year published 2017
volume 170
beginning page 83
ending page 94
digital object identifier (doi) 10.1016/j.chemosphere.2016.12.008
page count 12
web of science category Environmental Sciences
subject category Environmental Sciences & Ecology
document delivery number EJ0KK
unique article identifier WOS:000392897100011