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title Trichoderma harzianum T1A constitutively secretes proteins involved in the biological control of Guignardia citricarpa
authors de Lima, FB; Félix, C; Osório, N; Alves, A; Vitorino, R; Domingues, P; Ribeiro, RTD; Esteves, AC
author full name de Lima, Fernanda Blauth; Felix, Carina; Osorio, Nadia; Alves, Artur; Vitorino, Rui; Domingues, Pedro; da Silva Ribeiro, Rute. T.; Esteves, Ana Cristina
title Trichoderma harzianum T1A constitutively secretes proteins involved in the biological control of Guignardia citricarpa
nationality internacional
source BIOLOGICAL CONTROL
language English
document type Article
author keywords Extracellular proteome analysis; Biological control; Plant resistance; Citrus Black Spot disease
keywords plus AMINO-ACID OXIDASE; FUNGAL PLANT-PATHOGENS; CITRUS BLACK SPOT; CELL-WALL; RHIZOCTONIA-SOLANI; BOTRYTIS-CINEREA; ENCODING GENE; SKIN MUCUS; BIOCONTROL; RESISTANCE
abstract Citrus Black Spot caused by the fungus Guignardia citricarpa has an enormous economic impact. However, studies addressing the reduction or replacement of conventional fungicides for its control are scarce. Trichoderma harzianum is the most widely used species as a biological control agent. Nevertheless, only scarce reports describe the antagonism between T. harzianum and G. citricarpa. To investigate the mechanisms involved in the control of G. citricarpa, the proteins present in the secretome of T. harzianum TIA grown in medium containing deactivated mycelium of G. citricarpa were characterized and compared to a control condition. From the 178 significantly differentially expressed spots, 65 proteins were identified by mass spectrometry. Glycoside hydrolases, an L-amino acid oxidase, a serine protease and Epl 1 protein, represent 54% of the total proteins and are related not only to mycoparasitism, but also to plant defense response stimulation and biological control. When challenged by the pathogen, T. harzianum TIA reduces the total amount of secreted proteins, especially related to primary metabolism, increasing the secretion of proteins related to fungal interaction. Our results show, for the first time, that T. harzianum T1A secretes proteins related to the control of G. citricarpa, and induction of plant resistance, even in the absence of pathogen challenge. (C) 2017 Elsevier Inc. All rights reserved.
author address [de Lima, Fernanda Blauth; da Silva Ribeiro, Rute. T.] Univ Caxias do Sul, Dept Biotechnol, St Francisco Getulio Vargas 1130, BR-95070560 Caxias Do Sul, RS, Brazil; [Felix, Carina; Osorio, Nadia; Alves, Artur; Esteves, Ana Cristina] Univ Aveiro, Dept Biol, CESAM, P-3810193 Aveiro, Portugal; [Vitorino, Rui] Univ Aveiro, Inst Biomed iBiMED, Dept Med Sci, Aveiro, Portugal; [Vitorino, Rui] Univ Porto, Fac Med, Dept Physiol & Cardiothorac Surg, Oporto, Portugal; [Domingues, Pedro] Univ Aveiro, Dept Chem, QOPNA, P-3810193 Aveiro, Portugal
reprint address de Lima, FB (reprint author), Univ Caxias do Sul, Dept Biotechnol, St Francisco Getulio Vargas 1130, BR-95070560 Caxias Do Sul, RS, Brazil.
e-mail address nandalima85@gmail.com
orcid number Alves, Artur/0000-0003-0117-2958
funding agency and grant number FEDER; RNEM (Portuguese Mass Spectrometry Network); FCT [IF/00835/2013, BPD/102572/2014, BD/97613/2013, PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788, UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638, UID/BIM/04501/2013, UID/IC/00051/2013]; CAPES [PDSE 99999.011686/2013-08]; FAPERGS
funding text This study was partially supported by FEDER funding through COMPETE program and by national funding through FCT within the research project ALIEN (PTDC/AGR-PRO/2183/2014 - POCI-01-0145-FEDER-016788) and to CESAM (UID/AMB/50017/2013 - POCI-01-0145-FEDER-007638), UID/BIM/04501/2013, UID/IC/00051/2013 and RNEM (Portuguese Mass Spectrometry Network). A Alves (IF/00835/2013), AC Esteves (BPD/102572/2014) and C Felix (BD/97613/2013) acknowledge funding from FCT. F Lima acknowledges CAPES (PDSE 99999.011686/2013-08) for the PhD scholarship to develop part of the research project: Proteomic analysis of Trichoderma atroviride and Trichoderma harzianum against Guignardia citricarpa, pathogenic agent of Citrus Black Spot at the University of Aveiro and FAPERGS for the PhD scholarship in Biotechnology at the University of Caxias do Sul (Brazil).
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cited reference count 84
publisher ACADEMIC PRESS INC ELSEVIER SCIENCE
publisher city SAN DIEGO
publisher address 525 B ST, STE 1900, SAN DIEGO, CA 92101-4495 USA
issn 1049-9644
29-character source abbreviation BIOL CONTROL
iso source abbreviation Biol. Control
publication date MAR
year published 2017
volume 106
beginning page 99
ending page 109
digital object identifier (doi) 10.1016/j.biocontrol.2017.01.003
page count 11
web of science category Biotechnology & Applied Microbiology; Entomology
subject category Biotechnology & Applied Microbiology; Entomology
document delivery number EK2CK
unique article identifier WOS:000393734800013
link https://authors.elsevier.com/a/1UPHK3kk-a7hP6