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title In vitro effect of some fungicides on growth and aflatoxins production by Aspergillus flavus isolated from Capsicum powder
authors Santos, L; Marin, S; Sanchis, V; Ramos, AJ
author full name Santos, L.; Marin, S.; Sanchis, V.; Ramos, A. J.
title In vitro effect of some fungicides on growth and aflatoxins production by Aspergillus flavus isolated from Capsicum powder
nationality nacional
source FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
language English
document type Article
author keywords Aspergillus flavus; growth; aflatoxins; fungicides
keywords plus OCHRATOXIN-A; WATER ACTIVITY; CONTAMINATION; TEMPERATURE; PAPRIKA; PARASITICUS; PEPPER; RED; FUSARIUM; STRAINS
abstract The aim of this study was to determine the effect of some pre-harvest fungicides on growth and aflatoxin (AF) production of three Aspergillus flavus strains found in Capsicum powder. Each isolate, previously isolated from paprika, chilli and smoked paprika, was inoculated on yeast extract sucrose agar and on a 3% paprika extract agar medium supplemented with different fungicides and incubated at 20 and 30 degrees C during 7 days. Growth measurements were obtained on days 3, 5 and 7, and the AF production was determined on day 7. The significance of the effects of the factors (strain, medium, temperature, time and fungicides) and their interaction over colony diameter and AF production was determined. Temperature constrained the effectiveness of fungicides in reducing growth, the fungicides being most effective at 20 degrees C. The efficacy of the fungicides over AF production depended on the medium used and temperature. The most effective fungicides in inhibiting growth and AF production, regardless of the strain tested or applied conditions, were tebuconazole 25% and mancozeb 80% applied at a concentration of 0.75 and 3.5 g l(-1), respectively. Care should thus be taken in the choice of a suitable fungicide because their effectiveness may depend on intra-specific variation and temperature. Moreover, it is necessary to take into account that the most efficient fungicide in reducing growth is not always the best choice for pre-harvest treatments because it may promote AF production. Thus, the best fungicide is the one that can simultaneous prevent growth and AF production.
author address [Santos, L.; Marin, S.; Sanchis, V.; Ramos, A. J.] Univ Lleida, Dept Food Technol, XaRTA UTPV, E-25198 Lleida, Spain
reprint address Ramos, AJ (reprint author), Univ Lleida, Dept Food Technol, XaRTA UTPV, Av Alcalde Rovira Roure 191, E-25198 Lleida, Spain.
e-mail address ajramos@tecal.udl.es
researcherid number sanchis, vicente/B-3752-2011; Ramos, Antonio J./B-3934-2011; Marin, Sonia/B-8295-2011; Santos, Liliana/G-6478-2013; Marin, Sonia/G-1013-2012
orcid number sanchis, vicente/0000-0001-9889-8098; Ramos, Antonio J./0000-0002-2830-8299; Marin, Sonia/0000-0002-0714-6155; Santos, Liliana/0000-0002-4453-5766; Marin, Sonia/0000-0002-0714-6155
funding agency and grant number Spanish government [AGL2007-66416-C05-03]; Catalonian government (XaRTA - Reference Network on Food Technology)
funding text The authors are grateful to the Spanish (Project Number AGL2007-66416-C05-03) and Catalonian (XaRTA - Reference Network on Food Technology) governments for financial support. The authors would like to thank X. Ochoa de Eribe (research and development technician of SIPCAM INAGRA) and Bayer CropScience S. L. for supplying some of the fungicide samples, and also R. Miranda for the grammatical review.
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cited reference count 43
times cited 3
total times cited count (wos, bci, and cscd) 3
publisher TAYLOR & FRANCIS LTD
publisher city ABINGDON
publisher address 4 PARK SQUARE, MILTON PARK, ABINGDON OX14 4RN, OXON, ENGLAND
issn 1944-0049
29-character source abbreviation FOOD ADDIT CONTAM A
iso source abbreviation Food Addit. Contam. Part A-Chem.
year published 2011
volume 28
issue 1
beginning page 98
ending page 106
digital object identifier (doi) 10.1080/19440049.2010.529622
page count 9
web of science category Chemistry, Applied; Food Science & Technology; Toxicology
subject category Chemistry; Food Science & Technology; Toxicology
document delivery number 712JD
unique article identifier WOS:000286661900011