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title Phosphite shifts physiological and hormonal profile of Monterey pine and delays Fusarium circinatum progression
authors Cerqueira, A; Alves, A; Berenguer, H; Correia, B; Gomez-Cadenas, A; Diez, JJ; Monteiro, P; Pinto, G
author full name Cerqueira, Andreia; Alves, Artur; Berenguer, Helder; Correia, Barbara; Gomez-Cadenas, Aurelio; Javier Diez, Julio; Monteiro, Pedro; Pinto, Gloria
title Phosphite shifts physiological and hormonal profile of Monterey pine and delays Fusarium circinatum progression
nationality internacional
language English
document type Article
author keywords Forestry disease management; Pine pitch canker disease; Pinus radiata; Elicitors; Hormones; Plant performance
abstract Fusarium circinatum is the causal agent of pitch canker disease affecting Pinus spp. and Pseudotsuga menziesii worldwide. Under strict quarantine measures, alternative approaches for disease control are necessary. Phosphite (Phi) salts are known for their fungicidal activity and as plant resistance elicitors; however, its potential is yet to be acknowledged in the Pinus-F. circinatum model. The main aim of this study was to assess whether the application of a Phi-based commercial formulation would delay the progression of the pitch canker on Pinus radiata plants, and on the in vitro fungal growth. In vitro assays were performed using different Phi concentrations (1% and 4%) and a non-treated control (0%), and repeated in vivo using inoculated and non-inoculated plants. Plant physiological parameters and hormonal content were evaluated. Phi was effective at inhibiting in vitro mycelial growth in a dose dependent manner. Regardless of fungal inoculation, Phi application induced positive effects on plant performance, despite phytotoxic effects found at 4%. Fusarium circinatum infection led to a reduction in gas exchange and chlorophyll fluorescence (Fv/Fm and phi PSII), while proline and hormone (JA, ABA and SA) levels increased. Phi was effective in delaying disease symptom development in a dose dependent manner, concurrent with in vitro observations: gas exchange and chlorophyll fluorescence (Fv/Fm) were unaffected; proline, MDA and ABA decreased; electrolyte leakage and total soluble sugars increased. This suggests a direct (pathogen growth inhibition) and indirect (host defense priming) action of Phi, showing that Phi represents a potential strategy to control F. circinatum infection. (C) 2017 Elsevier Masson SAS. All rights reserved.
author address [Cerqueira, Andreia; Alves, Artur; Berenguer, Helder; Correia, Barbara; Monteiro, Pedro; Pinto, Gloria] Univ Aveiro, CESAM Ctr Environm & Marine Studies, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Gomez-Cadenas, Aurelio] Univ Jaume 1, Dept Ciencias Agrarias & Medio Nat, Castello La Plana 12071, Spain; [Javier Diez, Julio] Univ Valladolid, Sustainable Forest Management Res Inst, IN1A, Ave Madrid 44, Palencia, Spain; [Javier Diez, Julio] Univ Valladolid, Dept Plant Prod & Forest Resources, Ave Madrid 44, Palencia, Spain
reprint address Pinto, G (reprint author), Univ Aveiro, CESAM Ctr Environm & Marine Studies, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address gpinto@ua.pt
funding agency and grant number FEDER through COMPETE (Programa Operacional Fatores de Competitividade); National Funds through the Portuguese Foundation for Science and Technology (FCT) within the project URGENTpine [PTDC/AGR-FOR/2768/2014]; COST Action PINESTRENGTH - COST (European Cooperation in Science and Technology) [FP1406]; FEDER [POCI-01-0145-FEDER-016785, UID/AMB/50017]; FCT [IF/00835/2013, SFRH/BD/86448/2012, SFRH/BPD/101669/2014]
funding text This research was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade), by National Funds through the Portuguese Foundation for Science and Technology (FCT) within the project URGENTpine (PTDC/AGR-FOR/2768/2014), and by the COST Action PINESTRENGTH (FP1406), supported by COST (European Cooperation in Science and Technology). FCT/MEC, through national funds, and co-funding by the FEDER (POCI-01-0145-FEDER-016785), within the PT2020 Partnership Agreement and Compete 2020 provide financial support to CESAM (UID/AMB/50017). FCT also supported A. Alves (FCT Investigator Programme - IF/00835/2013), B Correia (PhD grant - SFRH/BD/86448/2012) and G Pinto (Post doc grant - SFRH/BPD/101669/2014).
cited references Ali Z., 2000, AUSTRALAS PLANT PATH, V29, P59; Arbona V, 2016, CURR ISSUES MOL BIOL, V19, P13; BATES LS, 1973, PLANT SOIL, V39, P205, DOI 10.1007/BF00018060; Berbegal M, 2015, FOREST PATHOL, V45, P525, DOI 10.1111/efp.12204; Berger S, 2007, J EXP BOT, V58, P4019, DOI 10.1093/jxb/erm298; Bolton M. D., 2009, PRIMARY METABOLISM P; Burra DD, 2014, BMC PLANT BIOL, V14, DOI 10.1186/s12870-014-0254-y; Chow PS, 2004, TREE PHYSIOL, V24, P1129; Cinelli T., 2015, PLANT DIS, V100; Dalio RJD, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0087860; de Rigo D., 2016, EUROPEAN ATLAS FORES, P8; Donoso A, 2015, PHYSIOL MOL PLANT P, V92, P42, DOI 10.1016/j.pmpp.2015.08.009; Durgbanshi A, 2005, J AGR FOOD CHEM, V53, P8437, DOI 10.1021/jf050884b; Escandon M, 2016, TREE PHYSIOL, V36, P63, DOI 10.1093/treephys/tpv127; Eshraghi L, 2011, PLANT PATHOL, V60, P1086, DOI 10.1111/j.1365-3059.2011.02471.x; Machinandiarena MF, 2012, J PLANT PHYSIOL, V169, P1417, DOI 10.1016/j.jplph.2012.05.005; Ganley RJ, 2009, CAN J FOREST RES, V39, P2246, DOI 10.1139/X09-131; Gao T, 2016, MOLECULES, V21, DOI 10.3390/molecules21060770; Gordon TR, 2015, CROP PROT, V73, P28, DOI 10.1016/j.cropro.2015.02.018; GUEST D, 1991, BIOL REV, V66, P159, DOI 10.1111/j.1469-185X.1991.tb01139.x; Hardy GES, 2001, AUSTRALAS PLANT PATH, V30, P133, DOI 10.1071/AP01012; HEATH RL, 1968, ARCH BIOCHEM BIOPHYS, V125, P189, DOI 10.1016/0003-9861(68)90654-1; IRIGOYEN JJ, 1992, PHYSIOL PLANTARUM, V84, P55, DOI 10.1034/j.1399-3054.1992.840109.x; Iturritxa E, 2013, FOREST PATHOL, V43, P488, DOI 10.1111/efp.12061; Jackson TJ, 2000, PLANT PATHOL, V49, P147, DOI 10.1046/j.1365-3059.2000.00422.x; King M, 2010, MOL GENET GENOMICS, V284, P425, DOI 10.1007/s00438-010-0579-7; Liu PQ, 2016, FUNCT PLANT BIOL, V43, P563, DOI 10.1071/FP16006; Martin-Rodrigues N, 2013, NEW PHYTOL, V198, P1215, DOI 10.1111/nph.12222; Martinez-Alvarez P, 2016, BIOL CONTROL, V94, P1, DOI 10.1016/j.biocontrol.2015.11.011; Martinez-Medina A, 2010, PHYTOPATHOLOGY, V100, P682, DOI 10.1094/PHYTO-100-7-0682; Massoud K, 2012, PLANT PHYSIOL, V159, P286, DOI 10.1104/pp.112.194647; Mitchell RG, 2013, NEW FOREST, V44, P443, DOI 10.1007/s11056-012-9355-3; Percival Glynn C., 2015, Arboricultural Journal, V37, P7, DOI 10.1080/03071375.2015.1017388; Pilbeam RA, 2000, AUSTRALAS PLANT PATH, V29, P86, DOI 10.1071/AP00016; Pilbeam RA, 2011, TREES-STRUCT FUNCT, V25, P1121, DOI 10.1007/s00468-011-0587-1; Qi PF, 2016, PHYSIOL MOL PLANT P, V93, P39, DOI 10.1016/j.pmpp.2015.12.004; Quinn GGP, 2002, EXPT DESIGN DATA ANA; Richardson D. M., 2000, ECOLOGY BIOGEOGRAPHY, P3; Rolny N, 2011, PLANT PHYSIOL BIOCH, V49, P1220, DOI 10.1016/j.plaphy.2011.06.010; Sanchez-Zabala J, 2013, MYCORRHIZA, V23, P627, DOI 10.1007/s00572-013-0500-4; Scott P, 2016, PHYSIOL PLANTARUM, V158, P124, DOI 10.1111/ppl.12442; Sims DA, 2002, REMOTE SENS ENVIRON, V81, P337, DOI 10.1016/S0034-4257(02)00010-X; Szabados L, 2010, TRENDS PLANT SCI, V15, P89, DOI 10.1016/j.tplants.2009.11.009; Verma V, 2016, BMC PLANT BIOL, V16, DOI 10.1186/s12870-016-0771-y; Wingfield MJ, 2008, AUSTRALAS PLANT PATH, V37, P319, DOI 10.1071/AP08036
cited reference count 45
publisher city PARIS
publisher address 23 RUE LINOIS, 75724 PARIS, FRANCE
issn 0981-9428
29-character source abbreviation PLANT PHYSIOL BIOCH
iso source abbreviation Plant Physiol. Biochem.
publication date MAY
year published 2017
volume 114
beginning page 88
ending page 99
digital object identifier (doi) 10.1016/j.plaphy.2017.02.020
page count 12
web of science category Plant Sciences
subject category Plant Sciences
document delivery number ES4LH
unique article identifier WOS:000399506300009
link http://dx.doi.org/10.1016/j.plaphy.2017.02.020