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title Phosphite shifts physiological and hormonal profile of Monterey pine and delays Fusarium circinatum progression
authors Cerqueira, A; Alves, A; Berenguer, H; Correia, B; Gomez-Cadenas, A; Diez, JJ; Monteiro, P; Pinto, G
author full name Cerqueira, Andreia; Alves, Artur; Berenguer, Helder; Correia, Barbara; Gomez-Cadenas, Aurelio; Javier Diez, Julio; Monteiro, Pedro; Pinto, Gloria
title Phosphite shifts physiological and hormonal profile of Monterey pine and delays Fusarium circinatum progression
nationality internacional
source PLANT PHYSIOLOGY AND BIOCHEMISTRY
language English
document type Article
author keywords Forestry disease management; Pine pitch canker disease; Pinus radiata; Elicitors; Hormones; Plant performance
keywords plus PHYTOPHTHORA-CINNAMOMI; EUCALYPTUS-MARGINATA; WATER-STRESS; RESISTANCE; RADIATA; RESPONSES; CONIFERS; PROLINE; DISEASE; ACID
abstract Fusarium circinatum is the causal agent of pitch canker disease affecting Pinus spp. and Pseudotsuga menziesii worldwide. Under strict quarantine measures, alternative approaches for disease control are necessary. Phosphite (Phi) salts are known for their fungicidal activity and as plant resistance elicitors; however, its potential is yet to be acknowledged in the Pinus-F. circinatum model. The main aim of this study was to assess whether the application of a Phi-based commercial formulation would delay the progression of the pitch canker on Pinus radiata plants, and on the in vitro fungal growth. In vitro assays were performed using different Phi concentrations (1% and 4%) and a non-treated control (0%), and repeated in vivo using inoculated and non-inoculated plants. Plant physiological parameters and hormonal content were evaluated. Phi was effective at inhibiting in vitro mycelial growth in a dose dependent manner. Regardless of fungal inoculation, Phi application induced positive effects on plant performance, despite phytotoxic effects found at 4%. Fusarium circinatum infection led to a reduction in gas exchange and chlorophyll fluorescence (Fv/Fm and phi PSII), while proline and hormone (JA, ABA and SA) levels increased. Phi was effective in delaying disease symptom development in a dose dependent manner, concurrent with in vitro observations: gas exchange and chlorophyll fluorescence (Fv/Fm) were unaffected; proline, MDA and ABA decreased; electrolyte leakage and total soluble sugars increased. This suggests a direct (pathogen growth inhibition) and indirect (host defense priming) action of Phi, showing that Phi represents a potential strategy to control F. circinatum infection. (C) 2017 Elsevier Masson SAS. All rights reserved.
author address [Cerqueira, Andreia; Alves, Artur; Berenguer, Helder; Correia, Barbara; Monteiro, Pedro; Pinto, Gloria] Univ Aveiro, CESAM Ctr Environm & Marine Studies, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal; [Gomez-Cadenas, Aurelio] Univ Jaume 1, Dept Ciencias Agrarias & Medio Nat, Castello La Plana 12071, Spain; [Javier Diez, Julio] Univ Valladolid, Sustainable Forest Management Res Inst, IN1A, Ave Madrid 44, Palencia, Spain; [Javier Diez, Julio] Univ Valladolid, Dept Plant Prod & Forest Resources, Ave Madrid 44, Palencia, Spain
reprint address Pinto, G (reprint author), Univ Aveiro, CESAM Ctr Environm & Marine Studies, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address gpinto@ua.pt
funding agency and grant number FEDER through COMPETE (Programa Operacional Fatores de Competitividade); National Funds through the Portuguese Foundation for Science and Technology (FCT) within the project URGENTpine [PTDC/AGR-FOR/2768/2014]; COST Action PINESTRENGTH - COST (European Cooperation in Science and Technology) [FP1406]; FEDER [POCI-01-0145-FEDER-016785, UID/AMB/50017]; FCT [IF/00835/2013, SFRH/BD/86448/2012, SFRH/BPD/101669/2014]
funding text This research was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade), by National Funds through the Portuguese Foundation for Science and Technology (FCT) within the project URGENTpine (PTDC/AGR-FOR/2768/2014), and by the COST Action PINESTRENGTH (FP1406), supported by COST (European Cooperation in Science and Technology). FCT/MEC, through national funds, and co-funding by the FEDER (POCI-01-0145-FEDER-016785), within the PT2020 Partnership Agreement and Compete 2020 provide financial support to CESAM (UID/AMB/50017). FCT also supported A. Alves (FCT Investigator Programme - IF/00835/2013), B Correia (PhD grant - SFRH/BD/86448/2012) and G Pinto (Post doc grant - SFRH/BPD/101669/2014).
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cited reference count 45
publisher ELSEVIER FRANCE-EDITIONS SCIENTIFIQUES MEDICALES ELSEVIER
publisher city PARIS
publisher address 23 RUE LINOIS, 75724 PARIS, FRANCE
issn 0981-9428
29-character source abbreviation PLANT PHYSIOL BIOCH
iso source abbreviation Plant Physiol. Biochem.
publication date MAY
year published 2017
volume 114
beginning page 88
ending page 99
digital object identifier (doi) 10.1016/j.plaphy.2017.02.020
page count 12
web of science category Plant Sciences
subject category Plant Sciences
document delivery number ES4LH
unique article identifier WOS:000399506300009
link http://dx.doi.org/10.1016/j.plaphy.2017.02.020