HOME  »  Members  »  Integrated  »  Post-Docs
 
title System-wide analysis of short-term response to high temperature in Pinus radiata
authors Escandon, M; Valledor, L; Pascual, J; Pinto, G; Canal, MJ; Meijon, M
author full name Escandon, Monica; Valledor, Luis; Pascual, Jesus; Pinto, Gloria; Jesus Canal, Maria; Meijon, Monica
title System-wide analysis of short-term response to high temperature in Pinus radiata
nationality internacional
source JOURNAL OF EXPERIMENTAL BOTANY
language English
document type Article
author keywords Biomarkers; heat acclimation; metabolomics; multivariate integrative analyses; pine; proteomics
keywords plus HEAT-STRESS; ABIOTIC STRESS; MEMBRANE-FLUIDITY; OXIDATIVE DAMAGE; PLANT TOLERANCE; GENE-EXPRESSION; CLIMATE-CHANGE; ABSCISIC-ACID; ARABIDOPSIS; THERMOTOLERANCE
abstract Pinus radiata seedlings, the most widely planted pine species in the world, were exposed to temperatures within a range mimicking future scenarios based on current models of heat increase. The short-term heat response in P. radiata was studied in detail by exploring the metabolome, proteome and targeted transcriptome. The use of complementary mass spectrometry techniques, GC-MS and LC-Orbitrap-MS, together with novel bioinformatics tools allowed the reliable quantification of 2,075 metabolites and 901 protein groups. Integrative analyses of different functional levels and plant physiological status revealed a complex molecular interaction network of positive and negative correlations between proteins and metabolites involved in short-term heat response, including three main physiological functions as: 1) A hormone subnetwork, where fatty acids, flavonoids and hormones presented a key role; 2) An oxidoreductase subnetwork, including several dehydrogenase and peroxidase proteins; and 3) A heat shock protein subnetwork, with numerous proteins that contain a HSP20 domain, all of which were overexpressed at the transcriptional level. Integrated analysis pinpointed the basic mechanisms underlying the short-term physiological reaction of P. radiata during heat response. This approach was feasible in forest species and unmasked two novel candidate biomarkers of heat resistance, PHO1 and TRANSCRIPTION FACTOR APFI, and a MITOCHONDRIAL SMALL HEAT SHOCK PROTEIN, for use in future breeding programs.
author address [Escandon, Monica; Valledor, Luis; Pascual, Jesus; Jesus Canal, Maria; Meijon, Monica] Univ Oviedo, Fac Biol, Dept BOS, Plant Physiol, Oviedo, Asturias, Spain; [Pinto, Gloria] Univ Aveiro, Dept Biol, Aveiro, Portugal; [Pinto, Gloria] Univ Aveiro, Ctr Environm & Marine Studies CESAM, Aveiro, Portugal
reprint address Canal, MJ; Meijon, M (reprint author), Univ Oviedo, Fac Biol, Dept BOS, Plant Physiol, Oviedo, Asturias, Spain.
e-mail address mjcanal@uniovi.es; meijonmonica@uniovi.es
researcherid number Canal, Maria Jesus/L-1005-2014; Pinto, GP/B-1271-2011; Escandon Martinez, Monica/V-2371-2017
orcid number Canal, Maria Jesus/0000-0002-1639-9672; Pinto, GP/0000-0001-7735-5131; Escandon Martinez, Monica/0000-0001-5686-106X; Meijon, Monica/0000-0003-1563-5554
funding agency and grant number Spanish Ministry of Economy and Competitiveness [AGL2011-27904, AGL2014-54995-P]; Government of the Principality of Asturias [FC-15-GRUPIN14-005]; FEDER through COMPETE [UID/AMB/50017/2013]; National Funds through the Portuguese Foundation for Science and Technology (FCT) [PTDC/AGR-FOR/2768/2014]; Severo Ochoa Program (Government of Principado de Asturias, Spain) [BP11117]; FPU (Ministry of Education, Spain) [AP2010-5857]; FCT (Fundacao para a Ciencia e a Tecnologia, Portugal) [SFRH/BPD/101669/2014]; Spanish Ministry of Economy and Competitiveness; Ramon y Cajal Programme [RYC-2014-14981, RYC-2015-17871]; FCT/MEC (Portugal) - FEDER, within the PT2020 Partnership Agreement and Compete [CESAM-UID/AMB/50017]
funding text We are grateful for assistance in the LC-Orbitrap-MS protein analysis from Dr Carlos Fuentes and SCAI services (University of Cordoba). This publication is an output of the Projects financed by the Spanish Ministry of Economy and Competitiveness (AGL2011-27904, AGL2014-54995-P), Government of the Principality of Asturias (FC-15-GRUPIN14-005), FEDER funding through COMPETE (project: UID/AMB/50017/2013) and by National Funds through the Portuguese Foundation for Science and Technology (FCT) within the Project PTDC/AGR-FOR/2768/2014. ME was supported by a fellowship from the Severo Ochoa Program (BP11117) (Government of Principado de Asturias, Spain). JP was supported by a fellowship from the FPU (AP2010-5857) (Ministry of Education, Spain). FCT (Fundacao para a Ciencia e a Tecnologia, Portugal) supported fellowships of GP (SFRH/BPD/101669/2014). The Spanish Ministry of Economy and Competitiveness supported MM and LV by Ramon y Cajal Programme (RYC-2014-14981 and RYC-2015-17871, respectively). FCT/MEC (Portugal) co-funding by the FEDER, within the PT2020 Partnership Agreement and Compete 2020 provide financial support to Centre for Environmental and Marine Studies (CESAM-UID/AMB/50017). No conflicts of interest declare.
cited references Acharya B, 2009, PLANT MOL BIOL, V69, P451, DOI 10.1007/s11103-008-9427-0; Ahuja I, 2010, TRENDS PLANT SCI, V15, P664, DOI 10.1016/j.tplants.2010.08.002; Alegre Sara, 2016, Data Brief, V7, P1477, DOI 10.1016/j.dib.2016.03.074; Alfonso M, 2001, PLANTA, V212, P573, DOI 10.1007/s004250000421; Allen CD, 2010, FOREST ECOL MANAG, V259, P660, DOI 10.1016/j.foreco.2009.09.001; Almeselmani M, 2006, PLANT SCI, V171, P382, DOI 10.1016/j.plantsci.2006.04.009; Bita CE, 2013, FRONT PLANT SCI, V4, DOI 10.3389/fpls.2013.00273; BRISSON LF, 1994, PLANT CELL, V6, P1703; Rossouw D, 2008, STAT APPL GENET MOL, V7; Gonzalez I, 2009, BIOINFORMATICS, V25, P2855; Cerny M, 2013, J EXP BOT, V64, P4193, DOI 10.1093/jxb/ert227; Chen H, 2011, BMC BIOINFORMATICS, V12, DOI 10.1186/1471-2105-12-35; Cline MS, 2007, NAT PROTOC, V2, P2366, DOI 10.1038/nprot.2007.324; Correia B, 2016, METABOLOMICS, V12, DOI 10.1007/s11306-016-1088-4; Correia B, 2014, PLANT PHYSIOL BIOCH, V82, P151, DOI 10.1016/j.plaphy.2014.05.016; Coumou D, 2013, ENVIRON RES LETT, V8, DOI 10.1088/1748-9326/8/3/034018; Cramer GR, 2011, BMC PLANT BIOL, V11, DOI 10.1186/1471-2229-11-163; Romero-Rodriguez MC, 2014, J PROTEOMICS, V105, P85, DOI 10.1016/j.jprot.2014.01.027; Dafny-Yelin M, 2008, PLANT MOL BIOL, V67, P363, DOI 10.1007/s11103-008-9326-4; Das A, 2016, ADV PLANT BREEDING S, P513; Duliere V, 2013, J CLIMATE, V26, P8556, DOI 10.1175/JCLI-D-12-00818.1; Escandon M, 2016, TREE PHYSIOL, V36, P63, DOI 10.1093/treephys/tpv127; Furuhashi T, 2012, J PLANT INTERACT, V7, P84, DOI 10.1080/17429145.2011.603059; Hall AE., 2000, CROP RESPONSES ENV; Hamburger D, 2002, PLANT CELL, V14, P889, DOI 10.1105/tpc.000745; Harrison MA, 2012, PHYTOHORMONES ABIOTI, p49 ; Hellemans J, 2007, GENOME BIOL, V8, DOI 10.1186/gb-2007-8-2-r19; Hemme D, 2014, PLANT CELL, V26, P4270, DOI 10.1105/tpc.114.130997; Hummel J, 2007, BMC BIOINFORMATICS, V8, DOI 10.1186/1471-2105-8-216; Jia J, 2017, NATURE, P1; Ketchen DJ, 1996, STRATEGIC MANAGE J, V17, P441, DOI 10.1002/(SICI)1097-0266(199606)17:6<441::AID-SMJ819>3.0.CO;2-G; Kolde R., 2015, PHEATMAP PRETTY HEAT; Kosova K, 2011, J PROTEOMICS, V74, P1301, DOI 10.1016/j.jprot.2011.02.006; Larkindale J, 2005, PLANT PHYSIOL, V138, P882, DOI 10.1104/pp.105.062257; Larkindale J, 2002, PLANT PHYSIOL, V128, P682, DOI 10.1104/pp.010320; Larkindale J, 2008, PLANT PHYSIOL, V146, P748, DOI 10.1104/pp.107.112060; Lopez-Ibanez J, 2016, NUCLEIC ACIDS RES, V44, pW201, DOI 10.1093/nar/gkw253; Los DA, 2004, BBA-BIOMEMBRANES, V1666, P142, DOI 10.1016/j.bbamem.2004.08.002; Martinez V, 2016, FRONT PLANT SCI, V7, DOI 10.3389/fpls.2016.00838; Meijon M, 2016, MOL ECOL, V25, P959, DOI 10.1111/mec.13525; Meiri D, 2009, PLANT J, V59, P387, DOI 10.1111/j.1365-313X.2009.03878.x; Mironov KS, 2012, BBA-BIOENERGETICS, V1817, P1352, DOI 10.1016/j.bbabio.2011.12.011; Mishkind M, 2009, PLANT J, V60, P10, DOI 10.1111/j.1365-313X.2009.03933.x; Murakami Y, 2000, SCIENCE, V287, P476, DOI 10.1126/science.287.5452.476; Pascual J, 2017, MOL CELL PROTEOMICS, V16, P485, DOI 10.1074/mcp.M116.059436; Pascual J, 2016, J PROTEOMICS, V143, P390, DOI 10.1016/j.jprot.2016.03.003; Pluskal T, 2010, BMC BIOINFORMATICS, V11, DOI 10.1186/1471-2105-11-395; Pulido P, 2012, MOL PLANT, V5, P964, DOI 10.1093/mp/sss088; Puranik S, 2012, TRENDS PLANT SCI, V17, P369, DOI 10.1016/j.tplants.2012.02.004; R Development Core Team R, 2015, LANG ENV STAT COMP; Reddy PP, 2014, PLOS ONE, V9, DOI 10.1371/journal.pone.0103186; Ribot C, 2008, PLANT PHYSIOL, V147, P696, DOI 10.1104/pp.108.119321; RStudio Team, 2016, RSTUDIO INT DEV ENV; Sanmiya K, 2004, FEBS LETT, V557, P265, DOI 10.1016/S0014-5793(03)01494-7; Sarkar NK, 2009, BMC GENOMICS, V10, DOI 10.1186/1471-2164-10-393; SMITH PK, 1985, ANAL BIOCHEM, V150, P76, DOI 10.1016/0003-2697(85)90442-7; Suzuki N, 2016, PLOS ONE, V11, DOI 10.1371/journal.pone.0147625; Thimm O, 2004, PLANT J, V37, P914, DOI 10.1111/j.1365-313X.2004.02016.x; Treutter D, 2005, PLANT BIOLOGY, V7, P581, DOI 10.1055/s-2005-873009; Valledor L, 2014, BIOTECHNOLOGY BIOFUE, V7, P171; Valledor L, 2014, PLANT J, V79, P173, DOI 10.1111/tpj.12546; Valledor L, 2014, METHODS MOL BIOL, V1072, P51, DOI 10.1007/978-1-62703-631-3_5; Valledor L, 2014, METHODS MOL BIOL, V1072, P347, DOI 10.1007/978-1-62703-631-3_25; Valledor L, 2011, J PROTEOMICS, V74, P1, DOI 10.1016/j.jprot.2010.07.007; Wahid A, 2006, J PLANT PHYSIOL, V163, P723, DOI 10.1016/j.jplph.2005.07.007; Wang WX, 2004, TRENDS PLANT SCI, V9, P244, DOI 10.1016/j.tplants.2004.03.006; Wanichthanarak W, 2015, BIOMARK INSIGHTS, V10, P1, DOI 10.4137/BMI.S29511; Wickham H, 2009, USE R, P1, DOI 10.1007/978-0-387-98141-3_1; Zimmerli C, 2012, PLANT J, V72, P199, DOI 10.1111/j.1365-313X.2012.05058.x
cited reference count 69
publisher OXFORD UNIV PRESS
publisher city OXFORD
publisher address GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND
issn 0022-0957
29-character source abbreviation J EXP BOT
iso source abbreviation J. Exp. Bot.
publication date JUN 15
year published 2017
volume 68
issue 13
beginning page 3629
ending page 3641
digital object identifier (doi) 10.1093/jxb/erx198
subject category 13
document delivery number Plant Sciences
unique article identifier Plant Sciences
CESAM authors