HOME  »  Members  »  Integrated  »  Post-Docs
title Highly conserved d-loop sequences in woolly mouse opossums Marmosa (Micoureus)
authors Rocha, RG; Leite, YLR; Ferreira, E; Justino, J; Costa, LP
author full name Rocha, Rita Gomes; Leite, Yuri Luiz Reis; Ferreira, Eduardo; Justino, Juliana; Costa, Leonora Pires
title Highly conserved d-loop sequences in woolly mouse opossums Marmosa (Micoureus)
nationality internacional
language English
document type Article
author keywords Didelphidae; control region; marsupials; mitochondrial DNA
abstract This study reports the occurrence of highly conserved d-loop sequences in the mitochondrial genome of the woolly mouse opossum genus Marmosa subgenus Micoureus (Mammalia, Didelphimorphia, Didelphidae). Sixty-six sequences of Marmosa (Micoureus) demerarae, Marmosa (Micoureus) constantiae, and Marmosa (Micoureus) paraguayanus were amplified using universal d-loop primers and virtually no genetic differences were detected within and among species. These sequences matched the control region of the mitochondrial marsupial genome. Analyses of qualitative aspects of these sequences revealed that their structural composition is very similar to the d-loop region of other didelphid species. However, the total lack of variability has not been reported from other closely related species. The data analyzed here support the occurrence of highly conserved d-loop sequences, and we found no support for the hypothesis that these sequences are d-loop-like nuclear pseudogenes. Furthermore, the control and flanking regions obtained with different primers corroborate the lack of variability of the d-loop sequences in the mitochondrial genome of Marmosa (Micoureus).
author address [Rocha, Rita Gomes; Ferreira, Eduardo] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Rocha, Rita Gomes; Ferreira, Eduardo] Univ Aveiro, CESAM, P-3810193 Aveiro, Portugal; [Rocha, Rita Gomes; Leite, Yuri Luiz Reis; Ferreira, Eduardo; Costa, Leonora Pires] Univ Fed Espirito Santo, Dept Ciencias Biol, Lab Mastozool & Biogeog, Vitoria 29043900, Espirito Santo, Spain; [Justino, Juliana] Univ Fed Espirito Santo, Nucleo Genet Aplicada Conservacao Biodiversidade, Lab Mastozool & Biogeog, Dept Ciencias Biol, Vitoria 29043900, Espirito Santo, Spain
reprint address Rocha, RG (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address rgrocha@ua.pt
researcherid number Leite, Yuri/D-4667-2009; Rocha, Rita/F-9845-2012; Costa, Leonora/G-4259-2011; Ferreira, Eduardo/G-2291-2010; CESAM, UA/M-3762-2015
orcid number Leite, Yuri/0000-0002-0248-8738; Rocha, Rita/0000-0002-4410-3648; Ferreira, Eduardo/0000-0003-0497-6118;
funding agency and grant number Critical Ecosystem Partnership Fund (CEPF); Fundacao Ciencia e Tecnologia [SFRH/BD/24767/2005, SFRH/BD/23191/2005]; Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
funding text We are grateful to Ana C. Loss for laboratory assistance. The molecular facility at UFES was established with funds from the Critical Ecosystem Partnership Fund (CEPF). This work was partially supported by two PhD grants from Fundacao Ciencia e Tecnologia (Program POPH-QREN) to Rita G. Rocha (ref: SFRH/BD/24767/2005) and Eduardo Ferreira (ref: SFRH/BD/23191/2005). Leonora P. Costa and Yuri L. R. Leite received research grant and fellowship support from Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil). We also thank an anonymous reviewer and the editor Sergios-Orestis Kolokotronis for valuable comments and suggestions on a previous version of this manuscript.
cited references Avise JC, 2009, J BIOGEOGR, V36, P3, DOI 10.1111/j.1365-2699.2008.02032.x; Bensasson D, 2001, TRENDS ECOL EVOL, V16, P314, DOI 10.1016/S0169-5347(01)02151-6; Bravi CM, 2006, HUMAN MITOCHONDRIAL, P31; Bruford Michael W., 1992, P225; Cracraft J, 1998, ANIM CONSERV, V1, P139, DOI 10.1111/j.1469-1795.1998.tb00021.x; DeWoody JA, 1999, J MOL EVOL, V48, P380, DOI 10.1007/PL00013154; Dias IMG, 2010, CONSERV GENET, V11, P1579, DOI 10.1007/s10592-009-9962-5; Douzery E, 1997, MOL BIOL EVOL, V14, P1154; Fumagalli L, 1997, MOL ECOL, V6, P1199, DOI 10.1046/j.1365-294X.1997.00298.x; Gaziev AI, 2010, MOL BIOL+, V44, P358, DOI 10.1134/S0026893310030027; Huchon D, 1999, MOL ECOL, V8, P1743, DOI 10.1046/j.1365-294x.1999.00768.x; KIMURA M, 1980, J MOL EVOL, V16, P111, DOI 10.1007/BF01731581; Lemos B, 1999, J HERED, V90, P543, DOI 10.1093/jhered/90.5.543; Librado P, 2009, BIOINFORMATICS, V25, P1451, DOI 10.1093/bioinformatics/btp187; LOPEZ JV, 1994, J MOL EVOL, V39, P174; Moritz C, 1997, MOL ECOL, V6, P925, DOI 10.1046/j.1365-294X.1997.00268.x; Cattony Neto PQ, 2009, THESIS U FEDERAL SAO; Nilsson MA, 2006, ZOOL SCR, V35, P387, DOI 10.1111/j.1463-6409.2006.00237.x; Nilsson MA, 2009, MITOCHONDR DNA, V20, P126, DOI 10.3109/19401730903180112; Patton James L., 2003, P63; Patton JL, 2000, B AM MUS NAT HIST, P1, DOI 10.1206/0003-0090(2000)244<0001:MOTRJA>2.0.CO;2; Perna NT, 1996, CURR BIOL, V6, P128, DOI 10.1016/S0960-9822(02)00441-4; Pope LC, 2000, MOL ECOL, V9, P2041, DOI 10.1046/j.1365-294X.2000.01110.x; Sbisa E, 1997, GENE, V205, P125, DOI 10.1016/S0378-1119(97)00404-6; Smit A, 1996, REPEATMASKER OPEN 3; SMITH MF, 1992, MOL BIOL EVOL, V9, P204; Steiner C, 2003, J MAMMAL, V84, P822, DOI 10.1644/BME-019; Tamura K, 2007, MOL BIOL EVOL, V24, P1596, DOI 10.1093/molbev/msm092; Triant DA, 2008, GENETICA, V132, P21, DOI 10.1007/s10709-007-9145-6; Triant DA, 2007, J MAMMAL, V88, P908, DOI 10.1644/06-MAMM-A-204R1.1; Triant DA, 2009, J MAMMAL, V90, P561, DOI 10.1644/08-MAMM-A-149R1.1; Voss RS, 2009, B AM MUS NAT HIST, P1; Zhang DX, 1996, MOL ECOL, V5, P295; Zhang DX, 1996, TRENDS ECOL EVOL, V11, P247, DOI 10.1016/0169-5347(96)10031-8; Zhang WP, 2006, J GENET, V85, P107, DOI 10.1007/BF02729016; ZISCHLER H, 1995, NATURE, V378, P489, DOI 10.1038/378489a0
cited reference count 36
publisher city ABINGDON
issn 1940-1736
29-character source abbreviation MITOCHONDR DNA
iso source abbreviation Mitochondrial DNA
publication date APR
year published 2012
volume 23
issue 2
beginning page 77
ending page 83
digital object identifier (doi) 10.3109/19401736.2012.660922
page count 7
web of science category Genetics & Heredity
subject category Genetics & Heredity
document delivery number 919JB
unique article identifier WOS:000302319200005