HOME  »  Members  »  Integrated  »  Post-Docs
title Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak
authors Correia, B.; Rodriguez, J.L.; Valledor, L.; Almeida, T.; Santos, C.; Canal, M.J.; Pinto, G.
author full name Correia, Barbara; Luis Rodriguez, Jose; Valledor, Luis; Almeida, Tania; Santos, Conceicao; Jesus Canal, Maria; Pinto, Gloria
title Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak
nationality internacional
source Journal of Plant Physiology
language English
document type Article
author keywords High temperature; Plant performance; Quercus suber; Recovery
abstract Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks' distribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 degrees C to 55 degrees C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant performance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 degrees C showed abundance alterations in the tested genes; at 45 degrees C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 degrees C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. (C) 2013 Elsevier GmbH. All rights reserved.
author address [Correia, Barbara; Santos, Conceicao; Pinto, Gloria] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Correia, Barbara; Santos, Conceicao; Pinto, Gloria] Univ Aveiro, CESAM Ctr Environm & Marine Studies, P-3810193 Aveiro, Portugal; [Luis Rodriguez, Jose; Jesus Canal, Maria] Univ Oviedo, Univ Inst Biotechnol Asturias IUBA, BOS Dept, EPIPHYSAGE Res Grp, Oviedo, Spain; [Valledor, Luis] Acad Sci Czech Republic, Global Change Res Ctr, Brno 60300, Czech Republic; [Almeida, Tania] Ctr Biotecnol Agr & Agroalimentar Alentejo CEBAL, Inst Politecn Beja IPBeja, P-7801908 Beja, Portugal; [Almeida, Tania] Univ Aveiro, Ctr Res Ceram & Composite Mat CICECO, P-3810193 Aveiro, Portugal
reprint address Pinto, G (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address gpinto@ua.pt
funding agency and grant number FEDER through COMPETE (Programa Operacional Fatores de Competitividade); FCT (Foundation for Science and Technology) [PTDC/AGR-CFL/112996/2009]; Human Potential Operational Programme (National Strategic Reference Framework); European Social Fund (EU); [SFRH/BD/86448/2012]; [SFRH/BD/44410/2008]
funding text This work was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade) and by the FCT (Foundation for Science and Technology) project PTDC/AGR-CFL/112996/2009. G Pinto is hired under the programme Ciencia 2008 (FCT, Portugal), co-funded by the Human Potential Operational Programme (National Strategic Reference Framework 2007-2013) and European Social Fund (EU). FCT supported the fellowships of B Correia (SFRH/BD/86448/2012) and T Almeida (SFRH/BD/44410/2008). The authors thank Armando Costa for his technical assistance and Dr. Monica Meijon and Dr. Nuria de Diego for their critical reviews.
cited references Ahuja I, 2010, TRENDS PLANT SCI, V15, P664, DOI 10.1016/j.tplants.2010.08.002; Allen CD, 2010, FOREST ECOL MANAG, V259, P660, DOI 10.1016/j.foreco.2009.09.001; Aragoncillo C, 2005, INVEST AGRAR-SIST R, V14, P307; Aranda I, 2007, TREE PHYSIOL, V27, P671; BATES LS, 1973, PLANT SOIL, V39, P205, DOI 10.1007/BF00018060; BERRY J, 1980, ANNU REV PLANT PHYS, V31, P491, DOI 10.1146/annurev.pp.31.060180.002423; Brosche M, 2005, GENOME BIOL, V6, DOI 10.1186/gb-2005-6-12-r101; Brosche M, 2010, ABIOTIC STRESS ADAPTATION IN PLANTS: PHYSIOLOGICAL, MOLECULAR AND GENOMIC FOUNDATION, P91, DOI 10.1007/978-90-481-3112-9_5; Chalker-Scott L, 1999, PHOTOCHEM PHOTOBIOL, V70, P1, DOI 10.1562/0031-8655(1999)070<0001:ESOAIP>2.3.CO;2; Chaves I, 2011, J PLANT PHYSIOL, V168, P1729, DOI 10.1016/j.jplph.2011.05.013; Chaves MM, 2009, ANN BOT-LONDON, V103, P551, DOI 10.1093/aob/mcn125; Correia B, 2013, PLOS ONE, V8, DOI 10.1371/journal.pone.0053543; Cosio C, 2009, J EXP BOT, V60, P391, DOI [10.1093/jxb/ern318, 10.1093/jxb/em318]; Daas C, 2008, ANN FOREST SCI, V65, DOI 10.1051/forest:2008002; Dao TTH, 2011, PHYTOCHEM REV, V10, P397, DOI 10.1007/s11101-011-9211-7; De Diego N, 2013, TREE PHYSIOL, V33, P69, DOI 10.1093/treephys/tps125; Delfine S, 2000, NEW PHYTOL, V146, P27, DOI 10.1046/j.1469-8137.2000.00612.x; Du J, 2012, PLANT OMICS, V5, P46; Elkahoui S, 2005, PLANT SCI, V168, P607, DOI 10.1016/j.plantsci.2004.09.006; Eryilmaz F., 2006, BIOTECHNOL BIOTECHNO, V20, P47; Faria T, 1996, TREE PHYSIOL, V16, P115; Gao C, 2009, PLANT MOL BIOL REP, V28, P77; Ghouil H, 2003, TREE PHYSIOL, V23, P1031; Girija C, 2002, ENVIRON EXP BOT, V47, P1, DOI 10.1016/S0098-8472(01)00096-X; Gould KS, 2002, PLANT CELL ENVIRON, V25, P1261, DOI 10.1046/j.1365-3040.2002.00905.x; Haldimann P, 2004, PLANT CELL ENVIRON, V27, P1169, DOI 10.1111/j.1365-3040.2004.01222.x; Hassinen VH, 2011, PLANT BIOLOGY, V13, P225, DOI 10.1111/j.1438-8677.2010.00398.x; Hidalgo PJ, 2008, FOREST ECOL MANAG, V255, P25, DOI 10.1016/j.foreco.2007.07.012; Hiraga S, 2001, PLANT CELL PHYSIOL, V42, P462, DOI 10.1093/pcp/pce061; Howell SH, 2013, ANNU REV PLANT BIOL, V64, P477, DOI 10.1146/annurev-arplant-050312-120053; Huve K, 2011, PLANT CELL ENVIRON, V34, P113, DOI 10.1111/j.1365-3040.2010.02229.x; Intergovernmental Panel on Climate Change (IPCC), 2007, CLIMATE CHANGE 2007; Jofre A, 2003, PLANTA, V217, P813, DOI 10.1007/s00425-003-1048-x; Kumar S, 2011, PLANT PHYSIOL BIOCH, V49, P404, DOI 10.1016/j.plaphy.2011.01.012; Lindner M, 2010, FOREST ECOL MANAG, V259, P698, DOI 10.1016/j.foreco.2009.09.023; Liu XZ, 2000, CROP SCI, V40, P503; Mir G, 2004, J EXP BOT, V55, P2483, DOI 10.1093/jxb/erh254; Oliveira G, 2001, AUST J PLANT PHYSIOL, V28, P471, DOI 10.1071/PP00150; OSTERYOUNG KW, 1994, J BIOL CHEM, V269, P28676; Passardi F, 2005, PLANT CELL REP, V24, P255, DOI 10.1007/s00299-005-0972-6; Pedrol N, 2000, J PLANT PHYSIOL, V157, P383; Pena-Rojas K., 2004, TREE PHYSIOL, V24, P813; Penuelas J, 2005, PLANT CELL ENVIRON, V28, P278, DOI 10.1111/j.1365-3040.2004.01250.x; Penuelas J, 2002, NEW PHYTOL, V155, P227, DOI 10.1046/j.1469-8137.2002.00457.x; Puigderrajols P, 2002, J EXP BOT, V53, P1445, DOI 10.1093/jexbot/53.373.1445; Ramakers C, 2003, NEUROSCI LETT, V339, P62, DOI 10.1016/S0304-3940(02)01423-4; Ramirez-Valiente JA, 2010, TREE PHYSIOL, V30, P618, DOI 10.1093/treephys/tpq013; Reddy AR, 2004, J PLANT PHYSIOL, V161, P1189, DOI 10.1016/j.jplph.2004.01.013; Sakuta M, 2000, J PLANT RES, V113, P327, DOI 10.1007/PL00013943; Salvucci ME, 2004, PLANT PHYSIOL, V134, P1460, DOI 10.1104/pp.103.038323; Scarascia-Mugnozza G, FOREST ECOL MANAGE; Schreiber U., 1995, ECOL STU AN, V100, P49; Shao L, 2007, J INTEGR PLANT BIOL, V49, P1341, DOI 10.1111/j.1672-9072.2007.00527.x; Sharkey TD, 2005, PLANT CELL ENVIRON, V28, P269, DOI 10.1111/j.1365-3040.2005.01324.x; Sims DA, 2002, REMOTE SENS ENVIRON, V81, P337, DOI 10.1016/S0034-4257(02)00010-X; Soler M, 2008, TREE PHYSIOL, V28, P743; Szabados L, 2010, TRENDS PLANT SCI, V15, P89, DOI 10.1016/j.tplants.2009.11.009; Untergasser A, 2007, NUCLEIC ACIDS RES, V35, pW71, DOI 10.1093/nar/gkm306; Vandesompele J, 2002, GENOME BIOL, V3, DOI 10.1186/gb-2002-3-7-research0034; Vaz M, 2012, TREES-STRUCT FUNCT, V26, P1145, DOI 10.1007/s00468-012-0691-x; Verbruggen N, 2008, AMINO ACIDS, V35, P753, DOI 10.1007/s00726-008-0061-6; Verdaguer D, 2003, PLANT CELL ENVIRON, V26, P1407, DOI 10.1046/j.1365-3040.2003.01065.x; Wahid A, 2012, RESPONSES MANAGEMENT, P135; Wahid A, 2007, ENVIRON EXP BOT, V61, P199, DOI 10.1016/j.envexpbot.2007.05.011; Wang WX, 2003, PLANTA, V218, P1, DOI 10.1007/s00425-003-1105-5; Xu ZZ, 2006, PLANTA, V224, P1080, DOI 10.1007/s00425-006-0281-5; Zhang Q, 2012, BIOL PLANTARUM, V56, P97, DOI 10.1007/s10535-012-0022-5; Zhou GK, 2006, J BIOCHEM MOL BIOL, V39, P595
cited reference count 68
publisher city JENA
publisher address OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
iso source abbreviation J PLANT PHYSIOL
publication date MAR 15
year published 2014
volume 171
issue 6
beginning page 399
ending page 406
digital object identifier (doi) 10.1016/j.jplph.2013.12.004
page count 8
web of science category Plant Sciences
subject category Plant Sciences
unique article identifier AD8FH
link http://www.sciencedirect.com/science/article/pii/S017616171300477X