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title Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak
authors Correia, B.; Rodriguez, J.L.; Valledor, L.; Almeida, T.; Santos, C.; Canal, M.J.; Pinto, G.
author full name Correia, Barbara; Luis Rodriguez, Jose; Valledor, Luis; Almeida, Tania; Santos, Conceicao; Jesus Canal, Maria; Pinto, Gloria
title Analysis of the expression of putative heat-stress related genes in relation to thermotolerance of cork oak
nationality internacional
source Journal of Plant Physiology
language English
document type Article
author keywords High temperature; Plant performance; Quercus suber; Recovery
keywords plus HIGH-TEMPERATURE STRESS; QUERCUS-SUBER; LIPID-PEROXIDATION; CLIMATE-CHANGE; HIGHER-PLANTS; PHENOTYPIC PLASTICITY; CHALCONE SYNTHASE; DIURNAL CHANGES; SHOCK PROTEINS; WATER-STRESS
abstract Cork oak (Quercus suber L.) is a research priority in the Mediterranean area and because of cork oaks' distribution these stands are experiencing daily stress. Based on projections of intensifying climate change and considering the key role of exploring the recovery abilities, cork oak seedlings were subjected to a cumulative temperature increase from 25 degrees C to 55 degrees C and subsequent recovery. CO2 assimilation rate, chlorophyll fluorescence, anthocyanins, proline and lipid peroxidation were used to evaluate plant performance, while the relative abundance of seven genes encoding for proteins of cork oak with a putative role in thermal/stress regulation (POX1, POX2, HSP10.4, HSP17a.22, CHS, MTL and RBC) was analyzed by qPCR (quantitative Polymerase Chain Reaction). A temperature change to 35 degrees C showed abundance alterations in the tested genes; at 45 degrees C, the molecular changes were associated with an antioxidant response, possibly modulated by anthocyanins. At 55 degrees C, HSP17a.22, MTL and proline accumulation were evident. After recovery, physiological balance was restored, whereas POX1, HSP10.4 and MTL abundances were suggested to be involved in increased thermotolerance. The data presented here are expected to pinpoint some pathways changes occurring during such stress and further recovery in this particular Mediterranean species. (C) 2013 Elsevier GmbH. All rights reserved.
author address [Correia, Barbara; Santos, Conceicao; Pinto, Gloria] Univ Aveiro, Dept Biol, P-3810193 Aveiro, Portugal; [Correia, Barbara; Santos, Conceicao; Pinto, Gloria] Univ Aveiro, CESAM Ctr Environm & Marine Studies, P-3810193 Aveiro, Portugal; [Luis Rodriguez, Jose; Jesus Canal, Maria] Univ Oviedo, Univ Inst Biotechnol Asturias IUBA, BOS Dept, EPIPHYSAGE Res Grp, Oviedo, Spain; [Valledor, Luis] Acad Sci Czech Republic, Global Change Res Ctr, Brno 60300, Czech Republic; [Almeida, Tania] Ctr Biotecnol Agr & Agroalimentar Alentejo CEBAL, Inst Politecn Beja IPBeja, P-7801908 Beja, Portugal; [Almeida, Tania] Univ Aveiro, Ctr Res Ceram & Composite Mat CICECO, P-3810193 Aveiro, Portugal
reprint address Pinto, G (reprint author), Univ Aveiro, Dept Biol, Campus Univ Santiago, P-3810193 Aveiro, Portugal.
e-mail address gpinto@ua.pt
funding agency and grant number FEDER through COMPETE (Programa Operacional Fatores de Competitividade); FCT (Foundation for Science and Technology) [PTDC/AGR-CFL/112996/2009]; Human Potential Operational Programme (National Strategic Reference Framework); European Social Fund (EU); [SFRH/BD/86448/2012]; [SFRH/BD/44410/2008]
funding text This work was supported by FEDER through COMPETE (Programa Operacional Fatores de Competitividade) and by the FCT (Foundation for Science and Technology) project PTDC/AGR-CFL/112996/2009. G Pinto is hired under the programme Ciencia 2008 (FCT, Portugal), co-funded by the Human Potential Operational Programme (National Strategic Reference Framework 2007-2013) and European Social Fund (EU). FCT supported the fellowships of B Correia (SFRH/BD/86448/2012) and T Almeida (SFRH/BD/44410/2008). The authors thank Armando Costa for his technical assistance and Dr. Monica Meijon and Dr. Nuria de Diego for their critical reviews.
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cited reference count 68
publisher ELSEVIER GMBH, URBAN & FISCHER VERLAG
publisher city JENA
publisher address OFFICE JENA, P O BOX 100537, 07705 JENA, GERMANY
iso source abbreviation J PLANT PHYSIOL
publication date MAR 15
year published 2014
volume 171
issue 6
beginning page 399
ending page 406
digital object identifier (doi) 10.1016/j.jplph.2013.12.004
page count 8
web of science category Plant Sciences
subject category Plant Sciences
unique article identifier AD8FH
link http://www.sciencedirect.com/science/article/pii/S017616171300477X