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  Ana Sofia Duarte  
  Phone: 234370970 | 234370774 (ext: 22710 | 22734)  
  e-mail: asduarte@ua.pt  
  Category: Invited Assistant Professor  
  Department: Department of Biology  
  Research group: Stress Biology
 
ISI Web of KnowledgeSM search factor: ResearcherID=A-5212-2009  
  Direct URL for this page: http://www.cesam.ua.pt/asduarte  
     
Molecular Microbiology Lab
Current activity

Post-doc researcher, financed by Fundação para a Ciência e a Tecnologia (SFRH/BPD/46290/2008), since 1 August 2009 and for 6 years (3+3). Invited Auxiliar Professor (58%) at the Health Sciences Department, Portuguese Catholic University.

Academic degrees

2006 – PhD in Biochemistry, (supervisors: Prof. Marlene Barros and Prof. Euclides Pires), University of Aveiro, Portugal.


2001 - MSc in Molecular Microbiology, (supervisors: Prof. Marlene Barros and Prof. Euclides Pires), University of Aveiro, Portugal.


1997 - Graduated in Biology, University of Aveiro, Portugal.

Professional activity

2012- presente- Invited Assistant Professor, Department of Biology, niversity of Aveiro.


2009-2012: Invited Assistant Professor (partial time, 58%), Department of Health Sciences, Portuguese Catholic University.


2009-2015: Pos-doc researcher, CESAM (FCT: SFRH/BPD/46290/2008).


2006-2009: Pos-doc researcher, CESAM (FCT: SFRH/BPD/25162/2005).


2001-2005: PhD scholarship, Department of Biology, University of Aveiro (FCT: SFRH/BD/2998/2000).


1998-2000: MSc scholarship, Department of Biology, University of Aveiro  (University of Aveiro and FCT, SFRH/BM/20661/99)


1998-1999- Natural Science High-school Teacher in "Escola Secundária de Vagos", 8th and 12th degrees.


1997-1998- Natural Science High-school Teacher in "Escola Secundária da Mealhada", for 7th, 8th and 10th degrees


1996-1997: PRODEP scholarship, Department of Biology, University of Aveiro (PRODEPII)


 

Research Interests

- Isolation and characterization of enzymes


- Cloning and protein expression/activity.


- Cytotoxicity and microbial virulence.


- Evaluation of genetic markers associated to antibiotic-resistance in bacteria


 

Research Projects

2010-2013: "SEAGULL: identification of nonpoint sources of faecal pollution in a natural environment: contributing data for risk assessment." (PTDC/AAC-AMB/109155/2008), (team member). PI: Dra. Anabela Pereira, University of Aveiro.

Post-graduated research supervision

PhD thesis


Osório, Nádia (2010-...): "Genome-wide Identification of Genetic Determinants of Antimicrobial Drug Resistance in Aeromonas hydrophila" (SFRH/BD/64343/2009), Department of Biology, University of Aveiro.


Cavaleiro, Eliana (2010-...): "A new approach towards the attenuation of virulence factor and biofilm formation by Aeromonas hydrophila and Aeromonas salmonicida" (SFRH/BD/47502/2008). Department of Biology, University of Aveiro and University of Cranfield (UK).


MSc Thesis


Custódio, Rafael (2008/2009): "Virulence characteristics of Aeromonas spp. in portuguese waters". Department of Biology, University of Aveiro.


Cavaleiro, Eliana (2007/2008): "Cloning and expression of a putative collagenase from Aeromonas hydrophila. Department of Biology, University of Aveiro.

Patents

2015. Patente “Método para produção de colagenase recombinante para digestão de colagénios”. Universidade de Aveiro. Inventores: Ana Sofia Duarte, Ana Cristina Esteves e António Correia. PCT/IB2014/065877. WO 2015068134 A1.

Course organization

2016. Advanced course on Scientific Writing and Communication. 4th edition. 27 Jun-1 Jul. Aveiro, Portugal.


2015. Advanced course on Scientific Writing and Communication. 3rd edition. 22-26 Jun. Aveiro, Portugal.


2014. Advanced course on Scientific Writing and Communication. 2nd edition. 28 Apr-02 May. Aveiro, Portugal.


2013. Advanced course on Scientific Writing and Communication. 11-14 Jun. Aveiro, Portugal.


2012. Advanced course on Protein Profiling of Microorganisms. 9-13 Jul. Aveiro, Portugal.

Scientific Supervision
PhD (Co-supervisor): Nádia Osório
(ex-CESAM member)
PhD (Co-supervisor): António C M Correia
(ex-CESAM member)
PhD (Co-supervisor): António C M Correia
(ex-CESAM member)
PhD (Co-supervisor): António C M Correia
(ex-CESAM member)
PhD (Co-supervisor): Nádia Osório
(ex-CESAM member)

Master (Main Supervisor): Rafael José Silva Custódio
(not a CESAM member)
Master (Main Supervisor): Sara Abreu
(not a CESAM member)
Master (Co-supervisor): Eliana Marisa dos Santos Cavaleiro
(ex-CESAM member)
Master (Co-supervisor): Inês Carolina Figueiredo Pereira
(not a CESAM member)

Pesquisa (Main Supervisor): Rodrigo Menezes
(not a CESAM member)
Pesquisa (Main Supervisor): Mafalda Tabuada
(not a CESAM member)
Pesquisa (Main Supervisor): Vanessa Ferreira (75679)
(not a CESAM member)
Pesquisa (Main Supervisor): Joana Magalhães (72279)
(not a CESAM member)
Pesquisa (Main Supervisor): Patrícia Oliveira (75661)
(not a CESAM member)
Pesquisa (Co-supervisor): João Baptista
(not a CESAM member)
Pesquisa (Co-supervisor): Bruna Pedrosa (75683)
(not a CESAM member)
Projeto de Licenciatura (Co-supervisor): Lúcia Isabel Paisano Sabala
(not a CESAM member)



Projects
Advanced Course on Protein Profiling of Microorganisms [see details]

Prometheus - PROteoME of Diplodia corticola, the cork oak paTHogEn fungUS [see details]

SEAGULL- Identification of nonpoint sources of faecal pollution in a natural environment: contributing data for risk assessment [see details]

Publications
  1. Duarte A.S., Correia A., Esteves A.C. (2016) Bacterial collagenases - A review. Critical Reviews In Microbiology. 42, 1, 106-126. (see details) ISI paper
    Link: http://informahealthcare.com/doi/abs/10.3109/1040841X.2014.904270
  2. Felix C., Duarte A.S., Vitorino R., Guerreiro A.C.L., Domingues P., Correia A.C.M., Alves A., Esteves A.C. (2016) Temperature Modulates the Secretome of the Phytopathogenic Fungus Lasiodiplodia theobromae. Frontiers In Plant Science. 7, (see details) ISI paper
    Link: http://dx.doi.org/10.3389/fpls.2016.01096
  3. Duarte A.S., Cavaleiro E., Pereira C., Merino S., Esteves A.C., Duarte E.P., Tomas J.M., Correia A.C. (2015) Aeromonas piscicola AH-3 expresses an extracellular collagenase with cytotoxic properties. Letters In Applied Microbiology. 60, 3, 288-297. (see details) ISI paper
    Link: http://onlinelibrary.wiley.com/doi/10.1111/lam.12373/abstract;jsessionid...
  4. Cavaleiro E., Duarte A.S., Esteves A.C., Correia A., Whitcombe M.J., Piletska E.V., Piletsky S.A., Chianella I. (2015) Novel Linear Polymers Able to Inhibit Bacterial Quorum Sensing. Macromolecular Bioscience. 15, 5, 647-656. (see details) ISI paper
    Link: http://dx.doi.org/10.1002/mabi.201400447
  5. Cruz A., Areias D., Duarte A., Correia A., Suzuki A., Mendo S. (2013) Aeromonas molluscorum Av27 is a potential tributyltin (TBT) bioremediator: phenotypic and genotypic characterization indicates its safe application. Antonie Van Leeuwenhoek. 104, 385-396. (see details) ISI paper
  6. Duarte AS, Duarte EP, Correia A, Pires E, Barros MT (2009) Cardosins improve neuronal regeneration after cell disruption: a comparative expression study. Cell Biol Toxicol. 25, 2, 99-108. (see details) ISI paper
  7. Duarte A., Rosa N., Duarte E., Pires E., Barros M. (2007) Cardosins: a new and efficient plant enzymatic tool to dissociate neuronal cells for the establishment of cell cultures. Biotechnol Bioeng. 97 , 4, 991-6. (see details) ISI paper
  8. Sarmento AC, Oliveira CS, Duarte AS, Pires E, Barros MT (2006) Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments - complex substrates hydrolysis. Enzyme And Microbial Technology. 38, 3/4, 415-421. (see details) ISI paper
  1. M.N. Capela, D.M. Tobaldi, C. Oliveira, A. Pereira, A.S. Duarte, M.P. Seabra, M.H.V. Fernandes (in press) Bioactivity and antibacterial activity against E. coli of calcium-phosphatebased glasses: Effect of silver content and crystallinity. Ceramics International. (see details)
  2. Duarte A.S., Pereira A., Cabrita A.S., Moir A., Pires E., Barros M. (2005) The Characterisation of the Collagenolytic Activity of Cardosin A Demonstrates its Potential Application for Extracellular Matrix Degradative Processes. Current Drug Discovery Technologies. 1, 2, 37-44. (see details)
  3. Pereira AO, Cartucho D, Duarte AS, Gil MH, Cabrita AS, Patrício JA, Barros MT (2005) Immobilisation of cardosin A in chitosan sponges as a novel Implant for drug delivery. Current Drug Discovery Technologies . 2, 4, 231-238. (see details)
  4. M.N. Capela, D.M. Tobaldi, C. Oliveira, A. Pereira, A.S. Duarte, M.P. Seabra, M.H.V. Fernandes Bioactivity and antibacterial activity against E. coli of calcium-phosphate based glasses: Effect of silver content and crystallinity. Ceramics International. (see details)
  1. Osório N (2015) Genome-wide Identification of Genetic Determinants of Antimicrobial Drug Resistance in Aeromonas hydrophila. PhD Thesis. (see details)
  2. Abreu,Sara Priscila Lopes de (2015) Collagenase for biotechnology applications. MSc Thesis. [supervisor] (see details)
  3. Cavaleiro E. (2014) A new approach towards the attenuation of virulence factor and biofilm formation by Aeromonas hydrophila and Aeromonas salmonicida. PhD Thesis. (see details)
  4. Filipe Honório (2012) Opportunistic pathogens: the case study of Aeromonas. MSc Thesis. [co-supervisor] (see details)
  5. Custódio R. (2009) Virulence characteristics of Aeromonas strains isolated from fish aquacultures. MSc Thesis. (see details)
    Link: http://ria.ua.pt/handle/10773/920
  6. Cavaleiro E. (2008) Cloning and expression of a putative collagenase from Aeromonas hydrophila. MSc Thesis. (see details)
    Link: http://ria.ua.pt/handle/10773/787
  7. Duarte A.S. (2006) Cardosinas no estabelecimento de culturas primárias de neurónios. PhD Thesis. (see details)
    Link: http://hdl.handle.net/10773/4616
  8. Duarte A.S. (2001) Utilização de uma enzima vegetal na prevenção de fibroses e aderências. MSc Thesis. (see details)
  1. Cavaleiro E., Duarte AS, Esteves AC, Correia A, Whitcombe MJ, Piletska EV, Piletsky SA, Chianella I (2015) Methacrylate-based Polymers to Inhibit Bacterial Quorum Sensing. (see details)
  2. Duarte, A.S., Vieira, S., da Cruz e Silva, O., Correia, A., Esteves, A. C. (2014) Production of a novel collagenase and its applications. (see details)
  3. Cavaleiro E., Guerreiro A., Whitcombe M. J., Piletsky A., Esteves A.C., Chianella I., Correia A., Duarte A.S. (2013) MIP nanoparticles: a strategy to block quorum sensing?. (see details)
  4. Osório N, Fernandes I, Devreese B, Esteves AC, Henriques I, Duarte AS, Correia A. (2013) Proteomic analysis of Aeromonas salmonicida CECT894T with experimentally induced resistance to ampicillin. (see details)
  5. Almeida R., Félix C., Duarte A.S., Alves A., Vitorino R., Correia A., Esteves A.C. (2013) Extracellular proteome of Diplodia corticola strains. (see details)
  6. Félix C., Duarte A.C., Alves A., Vitorino R., Correia A., Esteves A.C. (2013) The effect of temperature on the secretome of Lasiodiplodia theobromae. (see details)
  7. F Honório, I Fernandes, N Osório, B Devreese, AS Duarte, AC Esteves, A Correia (2013) Effect of thermal stress on the proteome of Aeromonas. (see details)
  8. Cavaleiro E., Cavaleiro E., , Whitcombe M.J.,, Whitcombe M.J., Piletska E., , Piletska E., Esteves A.C., , Esteves A.C., Piletsky S., Piletsky S., , Chianella I., Chianella I., , Correia A., , Correia A.C.M., Duarte A.S., Duarte A.S. (2012) New anti-microbial compounds: Aeromonas quorum sensing inhibition by methacrylate-based copolymers. (see details)
  9. Cavaleiro E., Guerreiro A., Poma A., Whitcombe M.J., Piletsky S., Correia A., Duarte A.S., Chianella I. (2012) MIP Nanoparticles (NPs) as new approach to fight bacterial diseases in aquaculture. (see details)
  10. Osório N, Eira D, Duarte AS, Correia A. (2012) New insights into the Aeromonas resistome. (see details)
  11. Cavaleiro E, Chianella I, Whitcombe MJ, Piletsky SA, Correia A, Duarte AS (2011) Synthetic polymers capable of suppressing virulence of bacterial fish pathogens. (see details)
  12. Duarte A.S., Cavaleiro E., Esteves A.C., Correia A.C.M. (2011) A novel collagenase from Aeromonas sp. (see details)
  13. Sabala L., Duarte A.S., Esteves A.C., Correia A.C.M. (2011) Purification and characterization of a recombinant histidine-tagged microbial collagenase. (see details)
  14. Felix C, Duarte AS, Alves A, Correia A, Esteves AC (2011) Why phytopathogenic fungi infect humans? The case of Lasiodiplodia theobromae. . (see details)
  15. Duarte, A.S., Amaro, S., Esteves, A.C., Correia, A.C. (2010) Diversity of extracellular proteases among Aeromonas. (see details)
  16. Duarte, A.S., Custódio, R., Amaro, S., Esteves, A.C., Correia, A.C. (2010) Aeromonas strains in aquacultures display diversified profiles of proteolytic activities. (see details)
  17. Custódio R, Correia A, Sarmento AC, Duarte AS (2009) Virulence traits presented by Aeromonas spp. isolated from fish farms and drinking waters. . (see details)
  18. Cavaleiro E. , Correia A., Duarte A.S. (2008) Effect of several environmental parameters on the expression of Aeromonas hydrophila gelatinases. (see details)
    Link: http://www.onlinebildiri.com/iums/includes/ongorunum.php?anum=1321
  19. Cavaleiro E. , Fonseca F., Correia A., Duarte A.S. (2008) Cloning and expression of a putative collagenase of Aeromonas hydrophila in Escherichia coli. (see details)
  20. Duarte A.S., Cavaleiro E., Correia A. (2007) Extracellular Gelatinases of Aeromonas sp - The Influence of Physicochemical Factors on Proteases Expression/Activity. (see details)
  21. Duarte AS, Duarte E, Pires E, Barros M (2006) Cardosins neuroprotection during brain dissagregation supports their potential application in cell based therapies. Poster session: New developments on polymers for tissue engineering - replacement and regeneration. (see details)
  22. Duarte AS, Duarte E, Pires E, Barros M (2005) Cardosins applied in neuronal embryonic cell culture: an efficient disaggregation tool. (see details)
  23. Duarte AS, Rosa N, Duarte E, Pires E, Barros (2004) Characterization of cardosins effects on neuronal embryonic cell culture - comparison with trypsin. (see details)
  24. Duarte AS, Rosa N, Duarte E, Pires E, Barros M (2002) ECM degradation by cardosins – Plant enzymes in prevention of fibrosis and in cells isolation. . (see details)
  25. Oliveira C. (2001) Conformational Study of cardosine A in organic solvents by limited proteolysis and FTIR spectroscopy. (see details)
  1. Cavaleiro E , Chianella I, Whitcombe MJ, Piletsky SA, Correia A, Duarte AS (2011) Synthetic polymers capable of suppressing virulence of bacterial fish pathogens. (see details)
  2. Duarte AS, Duarte E, Pires E, Rosa N, Barros M (2005) Cardosins applied in neuronal embryonic cell culture: an efficient desegregation tool. EUROPEAN JOURNAL OF NEUROLOGY Vol. 12, 292-292. (see details)
  3. Pereira A. , Duarte A.S. , Gil H. , Pires E. , Barros M. (2001) Cardosin A immobilised on chitosan. Arquivos de Patologia e Anatomia Patológica da Universidade de Coimbra,. (see details)
  4. Duarte A.S., Pereira A., Gil H. , Pires E., Cabrita S., Barros M . (2001) Potential Mechanisms of cardosin A action as a new anti-fibrosis agent. Arquivos de Patologia e Anatomia Patológica da Universidade de Coimbra. Vol. 33, 2001-supp, C8. (see details)
  5. Duarte A.S. , Pereira A., Cabrita S. , Moir A. , Pires E., Barros M. (2001) Utilisation of a plant enzyme in prevention of fibrosis and adhesion. Arquivos de Patologia e Anatomia Patológica da Universidade de Coimbra, Vol. 33, 2001-supp, C3. (see details)
  6. Duarte A.S. , Cabrita S., Barros M., Pires E. (1997) Comparison of Hydrolytic Activity of Cardosins A and B on Collagen I. XVII International Congress of Biochemistry and Molecular Biology, FASEB JOURNAL 11 (9): A1395. (see details)
  7. Duarte A.S. , Cabrita S., Barros M., Pires E. (1997) Comparison of Hydrolytic Activity of Cardosins A and B on Collagen I. XVII International Congress of Biochemistry and Molecular Biology, FASEB JOURNAL 11 (9): A1395. (see details)
  8. Calvinho P., Duarte A.S., Barros M., Cabrita S., Pires E. (1997) Evaluation of the Hydrolytic Action of Cardosins on Interstitial Collagens. Journal of Molecular Medicine, 1997-May (Abstracts): Volume-75, nº 5, B131-132. (see details)