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Ana Sofia Duarte

Ana Sofia Duarte

Invited Assistant Professor

  • Department: Department of Biology
  • Research Group: Stress Biology
  • ISI Web of KnowledgeSM search factor : ResearcherID=A-5212-2009


Course organization

2016. Advanced course on Scientific Writing and Communication. 4th edition. 27 Jun-1 Jul. Aveiro, Portugal.


2015. Advanced course on Scientific Writing and Communication. 3rd edition. 22-26 Jun. Aveiro, Portugal.


2014. Advanced course on Scientific Writing and Communication. 2nd edition. 28 Apr-02 May. Aveiro, Portugal.


2013. Advanced course on Scientific Writing and Communication. 11-14 Jun. Aveiro, Portugal.


2012. Advanced course on Protein Profiling of Microorganisms. 9-13 Jul. Aveiro, Portugal.

Patents

2015. Patente “Método para produção de colagenase recombinante para digestão de colagénios”. Universidade de Aveiro. Inventores: Ana Sofia Duarte, Ana Cristina Esteves e António Correia. PCT/IB2014/065877. WO 2015068134 A1.

Post-graduated research supervision

PhD thesis


Osório, Nádia (2010-...): "Genome-wide Identification of Genetic Determinants of Antimicrobial Drug Resistance in Aeromonas hydrophila" (SFRH/BD/64343/2009), Department of Biology, University of Aveiro.


Cavaleiro, Eliana (2010-...): "A new approach towards the attenuation of virulence factor and biofilm formation by Aeromonas hydrophila and Aeromonas salmonicida" (SFRH/BD/47502/2008). Department of Biology, University of Aveiro and University of Cranfield (UK).


MSc Thesis


Custódio, Rafael (2008/2009): "Virulence characteristics of Aeromonas spp. in portuguese waters". Department of Biology, University of Aveiro.


Cavaleiro, Eliana (2007/2008): "Cloning and expression of a putative collagenase from Aeromonas hydrophila. Department of Biology, University of Aveiro.

Research Projects

2010-2013: "SEAGULL: identification of nonpoint sources of faecal pollution in a natural environment: contributing data for risk assessment." (PTDC/AAC-AMB/109155/2008), (team member). PI: Dra. Anabela Pereira, University of Aveiro.

Research Interests

- Isolation and characterization of enzymes


- Cloning and protein expression/activity.


- Cytotoxicity and microbial virulence.


- Evaluation of genetic markers associated to antibiotic-resistance in bacteria


 

Professional activity

2012- presente- Invited Assistant Professor, Department of Biology, niversity of Aveiro.


2009-2012: Invited Assistant Professor (partial time, 58%), Department of Health Sciences, Portuguese Catholic University.


2009-2015: Pos-doc researcher, CESAM (FCT: SFRH/BPD/46290/2008).


2006-2009: Pos-doc researcher, CESAM (FCT: SFRH/BPD/25162/2005).


2001-2005: PhD scholarship, Department of Biology, University of Aveiro (FCT: SFRH/BD/2998/2000).


1998-2000: MSc scholarship, Department of Biology, University of Aveiro  (University of Aveiro and FCT, SFRH/BM/20661/99)


1998-1999- Natural Science High-school Teacher in "Escola Secundária de Vagos", 8th and 12th degrees.


1997-1998- Natural Science High-school Teacher in "Escola Secundária da Mealhada", for 7th, 8th and 10th degrees


1996-1997: PRODEP scholarship, Department of Biology, University of Aveiro (PRODEPII)


 

Academic degrees

2006 – PhD in Biochemistry, (supervisors: Prof. Marlene Barros and Prof. Euclides Pires), University of Aveiro, Portugal.


2001 - MSc in Molecular Microbiology, (supervisors: Prof. Marlene Barros and Prof. Euclides Pires), University of Aveiro, Portugal.


1997 - Graduated in Biology, University of Aveiro, Portugal.

Current activity

Post-doc researcher, financed by Fundação para a Ciência e a Tecnologia (SFRH/BPD/46290/2008), since 1 August 2009 and for 6 years (3+3). Invited Auxiliar Professor (58%) at the Health Sciences Department, Portuguese Catholic University.

Molecular Microbiology Lab

Scientific Supervision

In progress
Concluded

Projects

In progress
Concluded
  • Advanced Course on Protein Profiling of Microorganisms See more
  • Prometheus - PROteoME of Diplodia corticola, the cork oak paTHogEn fungUS See more
  • SEAGULL- Identification of nonpoint sources of faecal pollution in a natural environment: contributing data for risk assessment See more

Publications

WoS Papers (12)
Sarmento AC, Oliveira CS, Duarte AS, Pires E, Barros MT (2006) Evaluation of cardosin A as a probe for limited proteolysis in non-aqueous environments - complex substrates hydrolysis. In: Molecular Microbiology Lab. Enzyme And Microbial Technology. 38, 3/4, 415-421.
Duarte A., Rosa N., Duarte E., Pires E., Barros M. (2007) Cardosins: a new and efficient plant enzymatic tool to dissociate neuronal cells for the establishment of cell cultures. In: Molecular Microbiology Lab. Biotechnol Bioeng. 97 , 4, 991-6.
Duarte AS, Duarte EP, Correia A, Pires E, Barros MT (2009) Cardosins improve neuronal regeneration after cell disruption: a comparative expression study. In: Molecular Microbiology Lab. Cell Biol Toxicol. 25, 2, 99-108.
Cruz A., Areias D., Duarte A., Correia A., Suzuki A., Mendo S. (2013) Aeromonas molluscorum Av27 is a potential tributyltin (TBT) bioremediator: phenotypic and genotypic characterization indicates its safe application. In: Molecular Microbiology Lab. Antonie Van Leeuwenhoek. 104, 385-396.
Duarte A.S., Correia A., Esteves A.C. (2016) Bacterial collagenases - A review. In: Molecular Microbiology Lab. Critical Reviews In Microbiology. 42, 1, 106-126.
Duarte A.S., Cavaleiro E., Pereira C., Merino S., Esteves A.C., Duarte E.P., Tomas J.M., Correia A.C. (2015) Aeromonas piscicola AH-3 expresses an extracellular collagenase with cytotoxic properties. In: Molecular Microbiology Lab. Letters In Applied Microbiology. 60, 3, 288-297.
Cavaleiro E., Duarte A.S., Esteves A.C., Correia A., Whitcombe M.J., Piletska E.V., Piletsky S.A., Chianella I. (2015) Novel Linear Polymers Able to Inhibit Bacterial Quorum Sensing. In: Molecular Microbiology Lab. Macromolecular Bioscience. 15, 5, 647-656.
Felix C., Duarte A.S., Vitorino R., Guerreiro A.C.L., Domingues P., Correia A.C.M., Alves A., Esteves A.C. (2016) Temperature Modulates the Secretome of the Phytopathogenic Fungus Lasiodiplodia theobromae. In: Molecular Microbiology Lab. Frontiers In Plant Science. 7,
Capela M.N., Tobaldi D.M., Oliveira C., Pereira A., Duarte A.S., Seabra M.P., Fernandes M.H.V. (2017) Bioactivity and antibacterial activity against E-coli of calcium-phosphate-based glasses: Effect of silver content and crystallinity. In: Molecular Microbiology Lab. Ceramics International. 43, 16, 13800-13809.
Capela M.N., Tobaldi D.M., Oliveira C., Pereira A., Duarte A.S., Seabra M.P., Fernandes M.H.V. (2017) Bioactivity and antibacterial activity against E. coli of calcium-phosphatebased glasses: Effect of silver content and crystallinity. In: Molecular Microbiology Lab. Ceramics International. 43, 16, 13800-13809.
Felix C., Liborio S., Nunes M., Felix R., Duarte A.S., Alves A., Esteves A.C. (2018) Lasiodiplodia theobromae as a Producer of Biotechnologically Relevant Enzymes. In: Molecular Microbiology Lab. International Journal Of Molecular Sciences. 19, 2,
Félix C., Salvatore M.M., DellaGreca M., Meneses R., Duarte A.S., Salvatore F., Naviglio D., Gallo M., Jorrín-Novo J.V., Alves A., Andolfi A., Esteves A.C. (in press) Production of toxic metabolites by two strains of Lasiodiplodia theobromae, isolated from a coconut tree and a human patient. In: Molecular Microbiology Lab. Mycologia.
Other Papers (2)
Duarte A.S., Pereira A., Cabrita A.S., Moir A., Pires E., Barros M. (2005) The Characterisation of the Collagenolytic Activity of Cardosin A Demonstrates its Potential Application for Extracellular Matrix Degradative Processes. In: Molecular Microbiology Lab. Current Drug Discovery Technologies. 1, 2, 37-44.
Pereira AO, Cartucho D, Duarte AS, Gil MH, Cabrita AS, Patrício JA, Barros MT (2005) Immobilisation of cardosin A in chitosan sponges as a novel Implant for drug delivery. In: Molecular Microbiology Lab. Current Drug Discovery Technologies . 2, 4, 231-238.
Books (0)
Book Chapter (0)
Thesis (8)
Duarte A.S. (2001) Utilização de uma enzima vegetal na prevenção de fibroses e aderências. In: Molecular Microbiology Lab. MSc Thesis.
Duarte A.S. (2006) Cardosinas no estabelecimento de culturas primárias de neurónios. In: Molecular Microbiology Lab. PhD Thesis.
Cavaleiro E. (2014) A new approach towards the attenuation of virulence factor and biofilm formation by Aeromonas hydrophila and Aeromonas salmonicida. In: Molecular Microbiology Lab. PhD Thesis.
Osório N (2015) Genome-wide Identification of Genetic Determinants of Antimicrobial Drug Resistance in Aeromonas hydrophila. In: Molecular Microbiology Lab. PhD Thesis.
Custódio R. (2009) Virulence characteristics of Aeromonas strains isolated from fish aquacultures. In: Molecular Microbiology Lab. MSc Thesis.
Cavaleiro E. (2008) Cloning and expression of a putative collagenase from Aeromonas hydrophila. In: Molecular Microbiology Lab. MSc Thesis.
Filipe Honório (2012) Opportunistic pathogens: the case study of Aeromonas. In: Molecular Microbiology Lab. MSc Thesis.
Abreu,Sara Priscila Lopes de (2015) Collagenase for biotechnology applications. In: Molecular Microbiology Lab. MSc Thesis.
Communications (25)
Duarte A.S., Cavaleiro E., Correia A. (2007) Extracellular Gelatinases of Aeromonas sp - The Influence of Physicochemical Factors on Proteases Expression/Activity. In: Molecular Microbiology Lab.
Cavaleiro E. , Correia A., Duarte A.S. (2008) Effect of several environmental parameters on the expression of Aeromonas hydrophila gelatinases. In: Molecular Microbiology Lab.
Cavaleiro E. , Fonseca F., Correia A., Duarte A.S. (2008) Cloning and expression of a putative collagenase of Aeromonas hydrophila in Escherichia coli. In: Molecular Microbiology Lab.
Cavaleiro E., Guerreiro A., Whitcombe M. J., Piletsky A., Esteves A.C., Chianella I., Correia A., Duarte A.S. (2013) MIP nanoparticles: a strategy to block quorum sensing?. In: Molecular Microbiology Lab.
Osório N, Fernandes I, Devreese B, Esteves AC, Henriques I, Duarte AS, Correia A. (2013) Proteomic analysis of Aeromonas salmonicida CECT894T with experimentally induced resistance to ampicillin. In: Molecular Microbiology Lab.
Almeida R., Félix C., Duarte A.S., Alves A., Vitorino R., Correia A., Esteves A.C. (2013) Extracellular proteome of Diplodia corticola strains. In: Molecular Microbiology Lab.
Félix C., Duarte A.C., Alves A., Vitorino R., Correia A., Esteves A.C. (2013) The effect of temperature on the secretome of Lasiodiplodia theobromae. In: Molecular Microbiology Lab.
Cavaleiro E., Cavaleiro E., , Whitcombe M.J.,, Whitcombe M.J., Piletska E., , Piletska E., Esteves A.C., , Esteves A.C., Piletsky S., Piletsky S., , Chianella I., Chianella I., , Correia A., , Correia A.C.M., Duarte A.S., Duarte A.S. (2012) New anti-microbial compounds: Aeromonas quorum sensing inhibition by methacrylate-based copolymers. In: Molecular Microbiology Lab.
Cavaleiro E., Guerreiro A., Poma A., Whitcombe M.J., Piletsky S., Correia A., Duarte A.S., Chianella I. (2012) MIP Nanoparticles (NPs) as new approach to fight bacterial diseases in aquaculture. In: Molecular Microbiology Lab.
Cavaleiro E, Chianella I, Whitcombe MJ, Piletsky SA, Correia A, Duarte AS (2011) Synthetic polymers capable of suppressing virulence of bacterial fish pathogens. In: Molecular Microbiology Lab.
Duarte A.S., Cavaleiro E., Esteves A.C., Correia A.C.M. (2011) A novel collagenase from Aeromonas sp. In: Molecular Microbiology Lab.
Sabala L., Duarte A.S., Esteves A.C., Correia A.C.M. (2011) Purification and characterization of a recombinant histidine-tagged microbial collagenase. In: Molecular Microbiology Lab.
Duarte, A.S., Amaro, S., Esteves, A.C., Correia, A.C. (2010) Diversity of extracellular proteases among Aeromonas. In: Molecular Microbiology Lab.
Duarte, A.S., Custódio, R., Amaro, S., Esteves, A.C., Correia, A.C. (2010) Aeromonas strains in aquacultures display diversified profiles of proteolytic activities. In: Molecular Microbiology Lab.
Oliveira C. (2001) Conformational Study of cardosine A in organic solvents by limited proteolysis and FTIR spectroscopy. In: Molecular Microbiology Lab.
Osório N, Eira D, Duarte AS, Correia A. (2012) New insights into the Aeromonas resistome. In: Molecular Microbiology Lab.
Felix C, Duarte AS, Alves A, Correia A, Esteves AC (2011) Why phytopathogenic fungi infect humans? The case of Lasiodiplodia theobromae. . In: Molecular Microbiology Lab.
Custódio R, Correia A, Sarmento AC, Duarte AS (2009) Virulence traits presented by Aeromonas spp. isolated from fish farms and drinking waters. . In: Molecular Microbiology Lab.
Duarte AS, Duarte E, Pires E, Barros M (2006) Cardosins neuroprotection during brain dissagregation supports their potential application in cell based therapies. Poster session: New developments on polymers for tissue engineering - replacement and regeneration. In: Molecular Microbiology Lab.
Duarte AS, Duarte E, Pires E, Barros M (2005) Cardosins applied in neuronal embryonic cell culture: an efficient disaggregation tool. In: Molecular Microbiology Lab.
Duarte AS, Rosa N, Duarte E, Pires E, Barros (2004) Characterization of cardosins effects on neuronal embryonic cell culture - comparison with trypsin. In: Molecular Microbiology Lab.
Duarte AS, Rosa N, Duarte E, Pires E, Barros M (2002) ECM degradation by cardosins – Plant enzymes in prevention of fibrosis and in cells isolation. . In: Molecular Microbiology Lab.
Cavaleiro E., Duarte AS, Esteves AC, Correia A, Whitcombe MJ, Piletska EV, Piletsky SA, Chianella I (2015) Methacrylate-based Polymers to Inhibit Bacterial Quorum Sensing. In: Molecular Microbiology Lab.
Duarte, A.S., Vieira, S., da Cruz e Silva, O., Correia, A., Esteves, A. C. (2014) Production of a novel collagenase and its applications. In: Molecular Microbiology Lab.
F Honório, I Fernandes, N Osório, B Devreese, AS Duarte, AC Esteves, A Correia (2013) Effect of thermal stress on the proteome of Aeromonas. In: Molecular Microbiology Lab.
Other Publications (8)
Pereira A. , Duarte A.S. , Gil H. , Pires E. , Barros M. (2001) Cardosin A immobilised on chitosan. Arquivos de Patologia e Anatomia Patológica da Universidade de Coimbra,. In: Molecular Microbiology Lab.
Duarte A.S., Pereira A., Gil H. , Pires E., Cabrita S., Barros M . (2001) Potential Mechanisms of cardosin A action as a new anti-fibrosis agent. Arquivos de Patologia e Anatomia Patológica da Universidade de Coimbra. Vol. 33, 2001-supp, C8. In: Molecular Microbiology Lab.
Duarte A.S. , Pereira A., Cabrita S. , Moir A. , Pires E., Barros M. (2001) Utilisation of a plant enzyme in prevention of fibrosis and adhesion. Arquivos de Patologia e Anatomia Patológica da Universidade de Coimbra, Vol. 33, 2001-supp, C3. In: Molecular Microbiology Lab.
Duarte A.S. , Cabrita S., Barros M., Pires E. (1997) Comparison of Hydrolytic Activity of Cardosins A and B on Collagen I. XVII International Congress of Biochemistry and Molecular Biology, FASEB JOURNAL 11 (9): A1395. In: Molecular Microbiology Lab.
Duarte A.S. , Cabrita S., Barros M., Pires E. (1997) Comparison of Hydrolytic Activity of Cardosins A and B on Collagen I. XVII International Congress of Biochemistry and Molecular Biology, FASEB JOURNAL 11 (9): A1395. In: Molecular Microbiology Lab.
Calvinho P., Duarte A.S., Barros M., Cabrita S., Pires E. (1997) Evaluation of the Hydrolytic Action of Cardosins on Interstitial Collagens. Journal of Molecular Medicine, 1997-May (Abstracts): Volume-75, nº 5, B131-132. In: Molecular Microbiology Lab.
Cavaleiro E , Chianella I, Whitcombe MJ, Piletsky SA, Correia A, Duarte AS (2011) Synthetic polymers capable of suppressing virulence of bacterial fish pathogens. In: Molecular Microbiology Lab.
Duarte AS, Duarte E, Pires E, Rosa N, Barros M (2005) Cardosins applied in neuronal embryonic cell culture: an efficient desegregation tool. EUROPEAN JOURNAL OF NEUROLOGY Vol. 12, 292-292. In: Molecular Microbiology Lab.

CESAM - Centro de Estudos do Ambiente e do Mar - Universidade de Aveiro

CESAM Funding: